Restriction Fragments for CHS3/YBR023C



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The currently selected gene/sequence is :

CHS3/YBR023C on chromosome II from coordinates 287925 to 284428.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30049.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + Cfr10I RCCGGY 4 8 . . 5 8 + HpaII CCGG 5 7 . . 7 10 + CviJI RGCY 8 8 . . 22 25 + DpnI GATC 23 23 . . 22 25 + BstKTI GATC 24 22 . . 22 25 + MboI GATC 21 25 . . 24 29 + Hpy178III* TCNNGA 25 27 . . 22 26 - BinI* GGATC 16 17 . . 39 43 + Hpy188I TCNGA 41 40 . . 44 47 + SetI ASST 47 43 . . 47 50 + MseI TTAA 47 49 . . 51 56 + Hpy178III* TCNNGA 52 54 . . 55 65 + Hin4I GAYNNNNNVTC 46 41 78 73 56 60 + TspDTI ATGAA 71 69 . . 58 62 + MboII GAAGA 70 69 . . 58 70 + BarI GAAGNNNNNNTAC 50 45 82 77 61 65 + PleI GAGTC 69 70 . . 61 65 + MlyI GAGTC 70 70 . . 61 65 + HinfI GANTC 61 64 . . 58 63 - Ksp632I* CTCTTC 53 56 . . 63 67 + BsmAI GTCTC 68 72 . . 55 65 - Hin4I GAYNNNNNVTC 78 73 46 41 70 74 + DdeI CTNAG 70 73 . . 73 76 + SetI ASST 76 72 . . 76 81 + Hpy178III* TCNNGA 77 79 . . 80 90 + Hin4I GAYNNNNNVTC 71 66 103 98 80 90 + Eam1105I GACNNNNNGTC 85 84 . . 83 87 + Tsp4CI* ACNGT 85 84 . . 84 90 + TspRI CASTGNN 90 81 . . 80 90 - Hin4I GAYNNNNNVTC 103 98 71 66 91 94 + CviJI RGCY 92 92 . . 93 97 + DdeI CTNAG 93 96 . . 93 97 + BspCNI CTCAG 106 104 . . 93 97 + BseMII CTCAG 107 105 . . 94 99 + Hpy178III* TCNNGA 95 97 . . 98 103 + SduI GDGCHC 102 98 . . 92 98 - Bpu10I CCTNAGC 93 96 . . 98 103 + HgiAI* GWGCWC 102 98 . . 102 105 + SetI ASST 105 101 . . 103 106 + MnlI CCTC 113 112 . . 115 118 + CviJI RGCY 116 116 . . 126 130 + TauI GCSGC 129 126 . . 126 130 + BlsI GCNGC 128 127 . . 126 130 + BisI GCNGC 127 128 . . 128 131 + CviJI RGCY 129 129 . . 128 131 + HaeIII GGCC 129 129 . . 128 132 + BmgT120I GGNCC 129 130 . . 128 132 + AsuI* GGNCC 128 131 . . 126 129 - AciI CCGC 126 128 . . 139 142 + CviJI RGCY 140 140 . . 153 158 + Hpy178III* TCNNGA 154 156 . . 177 182 + MstI* TGCGCA 179 179 . . 178 181 + Hin6I GCGC 178 180 . . 178 181 + GlaI GCGC 179 179 . . 178 181 + HhaI GCGC 180 178 . . 180 190 + MwoI GCNNNNNNNGC 186 183 . . 199 203 + TspDTI ATGAA 214 212 . . 201 205 + TfiI GAWTC 201 204 . . 201 205 + HinfI GANTC 201 204 . . 205 214 + MslI CAYNNNNRTG 209 209 . . 206 209 + SetI ASST 209 205 . . 207 211 + BssKI CCNGG 206 211 . . 207 211 + BseBI CCWGG 208 209 . . 207 211 + ScrFI CCNGG 208 209 . . 207 211 + EcoRII CCWGG 206 211 . . 204 208 - HphI GGTGA 196 195 . . 210 214 + FokI GGATG 223 227 . . 210 214 + BseGI GGATG 216 214 . . 220 224 + BccI CCATC 228 229 . . 227 230 + Csp6I GTAC 227 229 . . 227 230 + RsaI GTAC 228 228 . . 229 234 + Cfr10I RCCGGY 229 233 . . 229 234 + BetI* WCCGGW 229 233 . . 229 234 + AgeI ACCGGT 229 233 . . 230 233 + HpaII CCGG 230 232 . . 235 240 + Hpy166II GTNNAC 237 237 . . 246 249 + CviRI* TGCA 247 247 . . 253 257 + Hpy99I CGWCG 257 252 . . 255 259 + Hpy188I TCNGA 257 256 . . 254 259 - MmeI TCCRAC 235 233 . . 262 265 + CviJI RGCY 263 263 . . 262 267 + NlaIV GGNNCC 264 264 . . 263 273 + MwoI GCNNNNNNNGC 269 266 . . 270 273 + Hin6I GCGC 270 272 . . 270 273 + GlaI GCGC 271 271 . . 270 273 + HhaI GCGC 272 270 . . 272 282 + MwoI GCNNNNNNNGC 278 275 . . 280 283 + CviJI RGCY 281 281 . . 282 286 + DdeI CTNAG 282 285 . . 282 286 + BspCNI CTCAG 295 293 . . 282 286 + BseMII CTCAG 296 294 . . 284 290 + TspRI CASTGNN 290 281 . . 294 305 + CspCI CAANNNNNGTGG 282 280 317 315 295 300 + ApoI RAATTY 295 299 . . 296 299 + TspEI AATT 295 299 . . 303 308 + CfrI YGGCCR 303 307 . . 304 307 + CviJI RGCY 305 305 . . 304 307 + HaeIII GGCC 305 305 . . 305 309 + TauI GCSGC 308 305 . . 305 309 + BlsI GCNGC 307 306 . . 305 309 + BisI GCNGC 306 307 . . 306 316 + BsiYI* CCNNNNNNNGG 312 309 . . 306 309 + AciI CCGC 306 308 . . 307 310 + FnuDII* CGCG 308 308 . . 313 317 + TspGWI ACGGA 328 326 . . 316 325 + BsaBI GATNNNNATC 320 320 . . 319 322 + SetI ASST 322 318 . . 319 322 + CviJI RGCY 320 320 . . 319 322 + AluI AGCT 320 320 . . 337 341 + TspDTI ATGAA 352 350 . . 357 360 + CviJI RGCY 358 358 . . 354 358 - Hin4II* CCTTC 348 347 . . 361 365 - TspGWI ACGGA 351 349 . . 365 368 + MseI TTAA 365 367 . . 377 381 + TspDTI ATGAA 392 390 . . 381 384 + SetI ASST 384 380 . . 379 383 - Hin4II* CCTTC 373 372 . . 391 395 + MboII GAAGA 403 402 . . 391 396 + BbvII* GAAGAC 398 402 . . 388 391 - AciI CCGC 388 390 . . 397 402 + ApoI RAATTY 397 401 . . 398 401 + TspEI AATT 397 401 . . 412 416 + HphI GGTGA 424 423 . . 409 413 - BccI CCATC 403 404 . . 418 423 + Hpy166II GTNNAC 420 420 . . 424 427 + TspEI AATT 423 427 . . 427 432 + AsuII TTCGAA 428 430 . . 428 431 + TaqI TCGA 428 430 . . 430 434 + MboII GAAGA 442 441 . . 436 439 + SetI ASST 439 435 . . 436 439 + CviJI RGCY 437 437 . . 436 439 + AluI AGCT 437 437 . . 444 448 + BlsI GCNGC 446 445 . . 444 448 + BisI GCNGC 445 446 . . 444 448 + BbvI GCAGC 456 460 . . 444 448 + TseI GCWGC 444 447 . . 446 449 + CviJI RGCY 447 447 . . 472 475 + MseI TTAA 472 474 . . 490 500 + Hin4I GAYNNNNNVTC 481 476 513 508 490 500 - Hin4I GAYNNNNNVTC 513 508 481 476 512 517 + TatI WGTACW 512 516 . . 513 516 + Csp6I GTAC 513 515 . . 513 516 + RsaI GTAC 514 514 . . 515 519 + Tsp4CI* ACNGT 517 516 . . 523 527 - TspDTI ATGAA 513 511 . . 529 532 + TaiI ACGT 532 528 . . 529 532 + SetI ASST 532 528 . . 529 532 + MaeII ACGT 529 531 . . 536 541 + SduI GDGCHC 540 536 . . 536 541 + BseSI GKGCMC 540 536 . . 537 542 + NlaIV GGNNCC 539 539 . . 537 542 + HgiCI* GGYRCC 537 541 . . 539 545 + AarI CACCTGC 549 553 . . 540 543 + SetI ASST 543 539 . . 540 545 + BspMI ACCTGC 549 553 . . 549 552 + TspEI AATT 548 552 . . 566 570 + FokI GGATG 579 583 . . 566 570 + BseGI GGATG 572 570 . . 563 566 - AciI CCGC 563 565 . . 563 567 - FauI CCCGC 556 558 . . 567 571 - SfaNI GCATC 557 561 . . 576 580 - Hin4II* CCTTC 570 569 . . 586 597 + CspCI CAANNNNNGTGG 574 572 609 607 605 608 + SetI ASST 608 604 . . 613 616 + MseI TTAA 613 615 . . 615 618 + TspEI AATT 614 618 . . 629 634 + TatI WGTACW 629 633 . . 629 634 + Bsp1407I TGTACA 629 633 . . 630 633 + Csp6I GTAC 630 632 . . 630 633 + RsaI GTAC 631 631 . . 640 650 + MwoI GCNNNNNNNGC 646 643 . . 648 651 + CviJI RGCY 649 649 . . 653 658 + Hpy178III* TCNNGA 654 656 . . 676 680 + Tsp4CI* ACNGT 678 677 . . 690 695 + AsuII TTCGAA 691 693 . . 691 694 + TaqI TCGA 691 693 . . 699 702 + TaiI ACGT 702 698 . . 699 702 + SetI ASST 702 698 . . 699 702 + MaeII ACGT 699 701 . . 727 732 + ApoI RAATTY 727 731 . . 728 731 + TspEI AATT 727 731 . . 738 741 + TspEI AATT 737 741 . . 740 743 + MseI TTAA 740 742 . . 743 747 + Tsp4CI* ACNGT 745 744 . . 750 753 + CviJI RGCY 751 751 . . 755 759 + TspDTI ATGAA 770 768 . . 758 761 + TspEI AATT 757 761 . . 763 773 + Hin4I GAYNNNNNVTC 754 749 786 781 762 767 - BciVI GTATCC 756 755 . . 770 773 + MnlI CCTC 780 779 . . 773 776 + FnuDII* CGCG 774 774 . . 778 783 + BetI* WCCGGW 778 782 . . 779 782 + HpaII CCGG 779 781 . . 782 787 + Hpy166II GTNNAC 784 784 . . 782 787 + BssNAI GTATAC 784 784 . . 782 787 + AccI GTMKAC 783 785 . . 791 794 + TaiI ACGT 794 790 . . 791 794 + SetI ASST 794 790 . . 791 794 + MaeII ACGT 791 793 . . 802 806 + TfiI GAWTC 802 805 . . 802 806 + HinfI GANTC 802 805 . . 805 809 + Hpy188I TCNGA 807 806 . . 812 822 + BsiYI* CCNNNNNNNGG 818 815 . . 820 826 + DraII RGGNCCY 821 824 . . 820 825 + SduI GDGCHC 824 820 . . 820 825 + Bsp120I GGGCCC 820 824 . . 820 825 + NlaIV GGNNCC 822 822 . . 820 824 + BmgT120I GGNCC 821 822 . . 820 825 + HgiJII* GRGCYC 824 820 . . 820 825 + BseSI GKGCMC 824 820 . . 820 824 + AsuI* GGNCC 820 823 . . 820 825 + ApaI GGGCCC 824 820 . . 821 824 + CviJI RGCY 822 822 . . 821 824 + HaeIII GGCC 822 822 . . 821 825 + BmgT120I GGNCC 822 823 . . 821 825 + AsuI* GGNCC 821 824 . . 823 828 + SecI* CCNNGG 823 827 . . 824 828 + BssKI CCNGG 823 828 . . 824 828 + BseBI CCWGG 825 826 . . 824 828 + ScrFI CCNGG 825 826 . . 824 828 + EcoRII CCWGG 823 828 . . 829 833 + Hpy188I TCNGA 831 830 . . 830 838 + AlwNI CAGNNNCTG 835 832 . . 832 836 - SfaNI GCATC 822 826 . . 844 848 - SfaNI GCATC 834 838 . . 839 851 - BarI GAAGNNNNNNTAC 863 858 831 826 875 879 + MaeIII GTNAC 874 879 . . 878 882 + Tsp4CI* ACNGT 880 879 . . 887 890 + SetI ASST 890 886 . . 890 895 + PsiI TTATAA 892 892 . . 907 910 + TspEI AATT 906 910 . . 898 911 - AjuI GAANNNNNNNTTGG 923 918 891 886 910 914 - MboII GAAGA 902 901 . . 922 931 + MslI CAYNNNNRTG 926 926 . . 922 925 + FatI CATG 921 925 . . 922 925 + NlaIII CATG 925 921 . . 922 925 + CviAII CATG 922 924 . . 937 940 + TspEI AATT 936 940 . . 944 947 + FatI CATG 943 947 . . 944 947 + NlaIII CATG 947 943 . . 944 947 + CviAII CATG 944 946 . . 964 967 + MseI TTAA 964 966 . . 969 973 + TfiI GAWTC 969 972 . . 969 973 + HinfI GANTC 969 972 . . 972 977 + Hpy178III* TCNNGA 973 975 . . 979 982 + CviJI RGCY 980 980 . . 980 986 + SapI GCTCTTC 987 990 . . 976 980 - BccI CCATC 970 971 . . 981 986 + Ksp632I* CTCTTC 987 990 . . 984 989 + AsuII TTCGAA 985 987 . . 985 988 + TaqI TCGA 985 987 . . 982 986 - MboII GAAGA 974 973 . . 988 998 + FalI AAGNNNNNCTT 979 974 1011 1006 989 992 + CviJI RGCY 990 990 . . 988 998 - FalI AAGNNNNNCTT 1011 1006 979 974 1000 1004 + Tsp4CI* ACNGT 1002 1001 . . 1009 1012 + TspEI AATT 1008 1012 . . 1018 1022 + FokI GGATG 1031 1035 . . 1018 1022 + BseGI GGATG 1024 1022 . . 1019 1023 - BccI CCATC 1013 1014 . . 1025 1029 + Tsp4CI* ACNGT 1027 1026 . . 1033 1036 + TaiI ACGT 1036 1032 . . 1033 1036 + SetI ASST 1036 1032 . . 1033 1036 + MaeII ACGT 1033 1035 . . 1042 1046 + MboII GAAGA 1054 1053 . . 1049 1053 + BslFI GGGAC 1063 1067 . . 1051 1055 + HgaI GACGC 1060 1065 . . 1061 1065 + Tsp4CI* ACNGT 1063 1062 . . 1065 1070 + AhaIII* TTTAAA 1067 1067 . . 1066 1069 + MseI TTAA 1066 1068 . . 1072 1075 + TaqI TCGA 1072 1074 . . 1077 1080 + SetI ASST 1080 1076 . . 1077 1080 + CviJI RGCY 1078 1078 . . 1077 1080 + AluI AGCT 1078 1078 . . 1084 1089 + Hpy166II GTNNAC 1086 1086 . . 1098 1102 + FokI GGATG 1111 1115 . . 1098 1102 + BseGI GGATG 1104 1102 . . 1099 1103 - BccI CCATC 1093 1094 . . 1113 1118 + Ksp632I* CTCTTC 1119 1122 . . 1117 1122 + XbaI TCTAGA 1117 1121 . . 1117 1122 + Hpy178III* TCNNGA 1118 1120 . . 1114 1118 - MboII GAAGA 1106 1105 . . 1118 1121 + MaeI CTAG 1118 1120 . . 1134 1139 + PsiI TTATAA 1136 1136 . . 1143 1147 + Hpy99I CGWCG 1147 1142 . . 1145 1148 + TaiI ACGT 1148 1144 . . 1145 1148 + SetI ASST 1148 1144 . . 1145 1148 + MaeII ACGT 1145 1147 . . 1153 1162 + BsaBI GATNNNNATC 1157 1157 . . 1158 1163 + XhoII RGATCY 1158 1162 . . 1158 1163 + BglII AGATCT 1158 1162 . . 1159 1162 + DpnI GATC 1160 1160 . . 1159 1162 + BstKTI GATC 1161 1159 . . 1159 1162 + MboI GATC 1158 1162 . . 1164 1169 + Hpy178III* TCNNGA 1165 1167 . . 1161 1165 - MboII GAAGA 1153 1152 . . 1173 1178 - MmeI TCCRAC 1154 1152 . . 1182 1186 + FokI GGATG 1195 1199 . . 1182 1186 + BseGI GGATG 1188 1186 . . 1187 1190 + TaiI ACGT 1190 1186 . . 1187 1190 + SetI ASST 1190 1186 . . 1187 1190 + MaeII ACGT 1187 1189 . . 1189 1194 + Hpy166II GTNNAC 1191 1191 . . 1189 1194 + HindII GTYRAC 1191 1191 . . 1208 1211 + TaqI TCGA 1208 1210 . . 1218 1222 + MboII GAAGA 1230 1229 . . 1218 1223 + BbvII* GAAGAC 1225 1229 . . 1227 1232 + ApoI RAATTY 1227 1231 . . 1228 1231 + TspEI AATT 1227 1231 . . 1236 1239 + SetI ASST 1239 1235 . . 1243 1246 + DpnI GATC 1244 1244 . . 1243 1246 + BstKTI GATC 1245 1243 . . 1243 1246 + MboI GATC 1242 1246 . . 1270 1273 + FatI CATG 1269 1273 . . 1270 1273 + NlaIII CATG 1273 1269 . . 1270 1273 + CviAII CATG 1270 1272 . . 1271 1275 + TspDTI ATGAA 1286 1284 . . 1281 1284 + TspEI AATT 1280 1284 . . 1302 1305 + TspEI AATT 1301 1305 . . 1307 1310 + MseI TTAA 1307 1309 . . 1315 1319 + HphI GGTGA 1327 1326 . . 1321 1326 + Hpy166II GTNNAC 1323 1323 . . 1321 1326 + AccI GTMKAC 1322 1324 . . 1324 1328 + HinfI GANTC 1324 1327 . . 1324 1328 - PleI GAGTC 1318 1319 . . 1324 1328 - MlyI GAGTC 1318 1318 . . 1333 1337 + Tsp4CI* ACNGT 1335 1334 . . 1335 1339 + Hpy99I CGWCG 1339 1334 . . 1349 1352 + MnlI CCTC 1359 1358 . . 1351 1355 + Hpy188I TCNGA 1353 1352 . . 1391 1397 + TspRI CASTGNN 1397 1388 . . 1395 1399 + HphI GGTGA 1407 1406 . . 1401 1404 + TspEI AATT 1400 1404 . . 1403 1406 + MseI TTAA 1403 1405 . . 1405 1410 + ApoI RAATTY 1405 1409 . . 1406 1409 + TspEI AATT 1405 1409 . . 1408 1412 - TspDTI ATGAA 1398 1396 . . 1413 1416 + TspEI AATT 1412 1416 . . 1417 1422 + Cac8I GCNNGC 1419 1419 . . 1427 1431 - TspGWI ACGGA 1417 1415 . . 1430 1435 - MmeI TCCRAC 1411 1409 . . 1435 1439 + Tsp4CI* ACNGT 1437 1436 . . 1436 1441 + SfeI* CTRYAG 1436 1440 . . 1440 1443 + SetI ASST 1443 1439 . . 1440 1443 + CviJI RGCY 1441 1441 . . 1440 1443 + AluI AGCT 1441 1441 . . 1442 1445 + MaeI CTAG 1442 1444 . . 1452 1455 + SetI ASST 1455 1451 . . 1455 1458 + CviRI* TGCA 1456 1456 . . 1445 1456 - TstI CACNNNNNNTCC 1469 1464 1437 1432 1465 1470 + Hpy166II GTNNAC 1467 1467 . . 1498 1503 + EcoRV GATATC 1500 1500 . . 1502 1505 + TaqI TCGA 1502 1504 . . 1504 1507 - MnlI CCTC 1497 1496 . . 1504 1514 - Hin4I GAYNNNNNVTC 1527 1522 1495 1490 1519 1524 + SspI AATATT 1521 1521 . . 1528 1538 + BsaXI ACNNNNNCTCC 1518 1515 1548 1545 1533 1536 + SetI ASST 1536 1532 . . 1533 1536 + CviJI RGCY 1534 1534 . . 1533 1536 + AluI AGCT 1534 1534 . . 1537 1540 + MnlI CCTC 1547 1546 . . 1535 1540 - BseRI GAGGAG 1526 1524 . . 1551 1556 + XhoII RGATCY 1551 1555 . . 1552 1555 + DpnI GATC 1553 1553 . . 1552 1555 + BstKTI GATC 1554 1552 . . 1552 1555 + MboI GATC 1551 1555 . . 1555 1558 + MnlI CCTC 1565 1564 . . 1552 1556 - BinI* GGATC 1546 1547 . . 1565 1568 + CviJI RGCY 1566 1566 . . 1565 1568 + HaeIII GGCC 1566 1566 . . 1563 1566 - MnlI CCTC 1556 1555 . . 1593 1597 + BslFI GGGAC 1607 1611 . . 1597 1606 + MslI CAYNNNNRTG 1601 1601 . . 1597 1606 + OliI CACNNNNGTG 1601 1601 . . 1602 1607 + Cac8I GCNNGC 1604 1604 . . 1612 1615 + SetI ASST 1615 1611 . . 1636 1639 + SetI ASST 1639 1635 . . 1636 1639 + CviJI RGCY 1637 1637 . . 1636 1639 + AluI AGCT 1637 1637 . . 1652 1655 + TspEI AATT 1651 1655 . . 1655 1658 + MseI TTAA 1655 1657 . . 1660 1664 + TfiI GAWTC 1660 1663 . . 1660 1664 + HinfI GANTC 1660 1663 . . 1671 1676 + XhoII RGATCY 1671 1675 . . 1671 1676 + BglII AGATCT 1671 1675 . . 1672 1675 + DpnI GATC 1673 1673 . . 1672 1675 + BstKTI GATC 1674 1672 . . 1672 1675 + MboI GATC 1671 1675 . . 1674 1679 + XbaI TCTAGA 1674 1678 . . 1674 1679 + Hpy178III* TCNNGA 1675 1677 . . 1675 1678 + MaeI CTAG 1675 1677 . . 1680 1689 + MslI CAYNNNNRTG 1684 1684 . . 1681 1684 + SetI ASST 1684 1680 . . 1682 1685 + MnlI CCTC 1692 1691 . . 1686 1689 + FatI CATG 1685 1689 . . 1686 1689 + NlaIII CATG 1689 1685 . . 1686 1689 + CviAII CATG 1686 1688 . . 1694 1699 + Hpy166II GTNNAC 1696 1696 . . 1705 1709 - MboII GAAGA 1697 1696 . . 1713 1717 - TspDTI ATGAA 1703 1701 . . 1721 1724 - MnlI CCTC 1714 1713 . . 1760 1765 + Hpy178III* TCNNGA 1761 1763 . . 1762 1765 + TaqI TCGA 1762 1764 . . 1766 1770 + TspDTI ATGAA 1781 1779 . . 1776 1779 + SetI ASST 1779 1775 . . 1776 1779 + CviJI RGCY 1777 1777 . . 1776 1779 + AluI AGCT 1777 1777 . . 1779 1784 + TatI WGTACW 1779 1783 . . 1779 1784 + Bsp1407I TGTACA 1779 1783 . . 1780 1783 + Csp6I GTAC 1780 1782 . . 1780 1783 + RsaI GTAC 1781 1781 . . 1795 1799 + BccI CCATC 1803 1804 . . 1817 1820 + MnlI CCTC 1827 1826 . . 1814 1818 - FokI GGATG 1800 1804 . . 1814 1818 - BseGI GGATG 1813 1811 . . 1819 1822 + TaqI TCGA 1819 1821 . . 1839 1849 + Hin4I GAYNNNNNVTC 1830 1825 1862 1857 1840 1850 + BsaXI ACNNNNNCTCC 1830 1827 1860 1857 1844 1847 + MnlI CCTC 1854 1853 . . 1849 1859 + EcoNI CCTNNNNNAGG 1853 1854 . . 1849 1859 + BsiYI* CCNNNNNNNGG 1855 1852 . . 1852 1855 + Csp6I GTAC 1852 1854 . . 1852 1855 + RsaI GTAC 1853 1853 . . 1855 1859 + BssKI CCNGG 1854 1859 . . 1855 1859 + BseBI CCWGG 1856 1857 . . 1855 1859 + ScrFI CCNGG 1856 1857 . . 1855 1859 + EcoRII CCWGG 1854 1859 . . 1858 1862 + BinI* GGATC 1866 1867 . . 1859 1862 + DpnI GATC 1860 1860 . . 1859 1862 + BstKTI GATC 1861 1859 . . 1859 1862 + MboI GATC 1858 1862 . . 1862 1867 - BtgZI GCGATG 1847 1851 . . 1869 1873 + TfiI GAWTC 1869 1872 . . 1869 1873 + HinfI GANTC 1869 1872 . . 1869 1879 + Hin4I GAYNNNNNVTC 1860 1855 1892 1887 1872 1876 + Hpy188I TCNGA 1874 1873 . . 1875 1879 + PleI GAGTC 1883 1884 . . 1875 1879 + MlyI GAGTC 1884 1884 . . 1875 1879 + HinfI GANTC 1875 1878 . . 1878 1883 + Hpy178III* TCNNGA 1879 1881 . . 1869 1879 - Hin4I GAYNNNNNVTC 1892 1887 1860 1855 1882 1886 + TfiI GAWTC 1882 1885 . . 1882 1886 + HinfI GANTC 1882 1885 . . 1885 1888 + TaqI TCGA 1885 1887 . . 1886 1890 + Hpy99I CGWCG 1890 1885 . . 1890 1900 + Hin4I GAYNNNNNVTC 1881 1876 1913 1908 1899 1904 + Hpy178III* TCNNGA 1900 1902 . . 1890 1900 - Hin4I GAYNNNNNVTC 1913 1908 1881 1876 1897 1901 - FokI GGATG 1883 1887 . . 1897 1901 - BseGI GGATG 1896 1894 . . 1903 1908 + EcoRV GATATC 1905 1905 . . 1922 1928 + TspRI CASTGNN 1928 1919 . . 1923 1927 + BsrI ACTGG 1928 1926 . . 1943 1948 + NlaIV GGNNCC 1945 1945 . . 1953 1957 + TfiI GAWTC 1953 1956 . . 1953 1957 + HinfI GANTC 1953 1956 . . 1955 1959 - TspDTI ATGAA 1945 1943 . . 1972 1976 + MaeIII GTNAC 1971 1976 . . 1984 1988 + Hpy188I TCNGA 1986 1985 . . 1985 1989 + DdeI CTNAG 1985 1988 . . 1989 1993 + FokI GGATG 2002 2006 . . 1985 1989 - BspCNI CTCAG 1977 1975 . . 1985 1989 - BseMII CTCAG 1976 1974 . . 1989 1993 + BseGI GGATG 1995 1993 . . 1987 1990 - MnlI CCTC 1980 1979 . . 1991 1995 + TspDTI ATGAA 2006 2004 . . 1993 1997 + MboII GAAGA 2005 2004 . . 1993 1998 - Ksp632I* CTCTTC 1988 1991 . . 1996 1999 - MnlI CCTC 1989 1988 . . 2002 2005 + MseI TTAA 2002 2004 . . 2004 2014 + FalI AAGNNNNNCTT 1995 1990 2027 2022 2004 2014 - FalI AAGNNNNNCTT 2027 2022 1995 1990 2017 2021 + HinfI GANTC 2017 2020 . . 2017 2021 - PleI GAGTC 2011 2012 . . 2017 2021 - MlyI GAGTC 2011 2011 . . 2031 2042 + TstI CACNNNNNNTCC 2022 2017 2054 2049 2043 2048 + ApoI RAATTY 2043 2047 . . 2044 2047 + TspEI AATT 2043 2047 . . 2061 2065 - BccI CCATC 2055 2056 . . 2074 2078 - BccI CCATC 2068 2069 . . 2080 2087 + PacI TTAATTAA 2084 2082 . . 2080 2083 + MseI TTAA 2080 2082 . . 2082 2085 + TspEI AATT 2081 2085 . . 2084 2087 + MseI TTAA 2084 2086 . . 2088 2093 + SduI GDGCHC 2092 2088 . . 2088 2093 + HgiJII* GRGCYC 2092 2088 . . 2089 2092 + CviJI RGCY 2090 2090 . . 2091 2096 + BmeT110I CYCGRG 2092 2094 . . 2091 2096 + AvaI CYCGRG 2091 2095 . . 2112 2117 + Hpy178III* TCNNGA 2113 2115 . . 2111 2116 - Eco57MI CTGRAG 2096 2094 . . 2111 2116 - GsuI CTGGAG 2096 2094 . . 2133 2137 - BccI CCATC 2127 2128 . . 2137 2148 + DrdI GACNNNNNNGTC 2143 2141 . . 2140 2148 + Tth111I GACNNNGTC 2143 2144 . . 2146 2150 + Tsp45I GTSAC 2145 2150 . . 2146 2150 + MaeIII GTNAC 2145 2150 . . 2147 2151 - HphI GGTGA 2139 2138 . . 2154 2157 + SetI ASST 2157 2153 . . 2159 2163 + TspDTI ATGAA 2174 2172 . . 2165 2168 + MseI TTAA 2165 2167 . . 2169 2172 + SetI ASST 2172 2168 . . 2185 2190 + BtsI GCAGTG 2192 2190 . . 2186 2192 + TspRI CASTGNN 2192 2183 . . 2191 2195 + SfaNI GCATC 2200 2204 . . 2197 2200 + CviJI RGCY 2198 2198 . . 2216 2221 + BalI TGGCCA 2218 2218 . . 2216 2221 + CfrI YGGCCR 2216 2220 . . 2217 2220 + CviJI RGCY 2218 2218 . . 2217 2220 + HaeIII GGCC 2218 2218 . . 2230 2233 - AciI CCGC 2230 2232 . . 2230 2234 - FauI CCCGC 2223 2225 . . 2251 2255 + TfiI GAWTC 2251 2254 . . 2251 2255 + HinfI GANTC 2251 2254 . . 2259 2262 + TspEI AATT 2258 2262 . . 2280 2283 + TaiI ACGT 2283 2279 . . 2280 2283 + SetI ASST 2283 2279 . . 2280 2283 + MaeII ACGT 2280 2282 . . 2280 2285 + AflIII ACRYGT 2280 2284 . . 2284 2288 - BslFI GGGAC 2269 2273 . . 2297 2302 + MfeI CAATTG 2297 2301 . . 2298 2301 + TspEI AATT 2297 2301 . . 2312 2315 + Csp6I GTAC 2312 2314 . . 2312 2315 + RsaI GTAC 2313 2313 . . 2309 2312 - AciI CCGC 2309 2311 . . 2318 2323 + PstI CTGCAG 2322 2318 . . 2318 2323 + SfeI* CTRYAG 2318 2322 . . 2319 2322 + CviRI* TGCA 2320 2320 . . 2329 2334 + NlaIV GGNNCC 2331 2331 . . 2330 2334 + BmgT120I GGNCC 2331 2332 . . 2330 2334 + AsuI* GGNCC 2330 2333 . . 2331 2334 + CviJI RGCY 2332 2332 . . 2331 2334 + HaeIII GGCC 2332 2332 . . 2332 2336 + TauI GCSGC 2335 2332 . . 2332 2336 + BlsI GCNGC 2334 2333 . . 2332 2336 + BisI GCNGC 2333 2334 . . 2333 2336 + AciI CCGC 2333 2335 . . 2341 2345 + BssKI CCNGG 2340 2345 . . 2341 2345 + ScrFI CCNGG 2342 2343 . . 2341 2345 + CauII* CCSGG 2342 2343 . . 2342 2345 + HpaII CCGG 2342 2344 . . 2345 2349 + MaeIII GTNAC 2344 2349 . . 2352 2355 + SetI ASST 2355 2351 . . 2351 2354 - MnlI CCTC 2344 2343 . . 2362 2366 + TfiI GAWTC 2362 2365 . . 2362 2366 + HinfI GANTC 2362 2365 . . 2370 2373 + TspEI AATT 2369 2373 . . 2376 2380 + Hpy188I TCNGA 2378 2377 . . 2408 2412 + TspDTI ATGAA 2423 2421 . . 2418 2422 + HinfI GANTC 2418 2421 . . 2418 2422 - PleI GAGTC 2412 2413 . . 2418 2422 - MlyI GAGTC 2412 2412 . . 2422 2427 + MfeI CAATTG 2422 2426 . . 2423 2426 + TspEI AATT 2422 2426 . . 2428 2433 + ApoI RAATTY 2428 2432 . . 2429 2432 + TspEI AATT 2428 2432 . . 2435 2438 + SetI ASST 2438 2434 . . 2435 2438 + CviJI RGCY 2436 2436 . . 2435 2438 + AluI AGCT 2436 2436 . . 2439 2444 + AhaIII* TTTAAA 2441 2441 . . 2440 2443 + MseI TTAA 2440 2442 . . 2446 2451 + SspI AATATT 2448 2448 . . 2465 2468 + CviJI RGCY 2466 2466 . . 2488 2492 + Tsp4CI* ACNGT 2490 2489 . . 2491 2494 + Csp6I GTAC 2491 2493 . . 2491 2494 + RsaI GTAC 2492 2492 . . 2503 2507 - SfaNI GCATC 2493 2497 . . 2509 2519 - BaeI ACNNNNGTAYC 2534 2529 2501 2496 2523 2528 + Hpy178III* TCNNGA 2524 2526 . . 2527 2531 - SfaNI GCATC 2517 2521 . . 2533 2536 + MseI TTAA 2533 2535 . . 2539 2544 + NdeI CATATG 2540 2542 . . 2558 2561 + MseI TTAA 2558 2560 . . 2555 2560 - TsoI TARCCA 2545 2543 . . 2562 2567 + XhoII RGATCY 2562 2566 . . 2563 2566 + DpnI GATC 2564 2564 . . 2563 2566 + BstKTI GATC 2565 2563 . . 2563 2566 + MboI GATC 2562 2566 . . 2563 2567 - BinI* GGATC 2557 2558 . . 2571 2581 + Hin4I GAYNNNNNVTC 2562 2557 2594 2589 2571 2581 - Hin4I GAYNNNNNVTC 2594 2589 2562 2557 2587 2591 + HphI GGTGA 2599 2598 . . 2590 2594 - BsmAI GTCTC 2584 2588 . . 2590 2595 - Eco31I GGTCTC 2584 2588 . . 2598 2601 + DpnI GATC 2599 2599 . . 2598 2601 + BstKTI GATC 2600 2598 . . 2598 2601 + MboI GATC 2597 2601 . . 2602 2612 + MwoI GCNNNNNNNGC 2608 2605 . . 2623 2627 + MaeIII GTNAC 2622 2627 . . 2628 2631 + CviRI* TGCA 2629 2629 . . 2631 2634 + TspEI AATT 2630 2634 . . 2645 2650 + TatI WGTACW 2645 2649 . . 2645 2650 + ScaI AGTACT 2647 2647 . . 2646 2649 + Csp6I GTAC 2646 2648 . . 2646 2649 + RsaI GTAC 2647 2647 . . 2658 2662 + SfaNI GCATC 2667 2671 . . 2667 2670 + CviJI RGCY 2668 2668 . . 2672 2677 + HindIII AAGCTT 2672 2676 . . 2673 2676 + SetI ASST 2676 2672 . . 2673 2676 + CviJI RGCY 2674 2674 . . 2673 2676 + AluI AGCT 2674 2674 . . 2680 2684 + TfiI GAWTC 2680 2683 . . 2680 2684 + HinfI GANTC 2680 2683 . . 2687 2691 - MboII GAAGA 2679 2678 . . 2686 2691 - BbvII* GAAGAC 2679 2683 . . 2698 2702 + MaeIII GTNAC 2697 2702 . . 2710 2714 + BssKI CCNGG 2709 2714 . . 2710 2713 + HpaII CCGG 2710 2712 . . 2710 2714 + ScrFI CCNGG 2711 2712 . . 2710 2714 + CauII* CCSGG 2711 2712 . . 2713 2717 + FokI GGATG 2726 2730 . . 2713 2717 + BseGI GGATG 2719 2717 . . 2748 2751 + SetI ASST 2751 2747 . . 2752 2756 + Hpy188I TCNGA 2754 2753 . . 2755 2759 - BccI CCATC 2749 2750 . . 2757 2762 - TsoI TARCCA 2747 2745 . . 2766 2771 + KpnI GGTACC 2770 2766 . . 2766 2771 + NlaIV GGNNCC 2768 2768 . . 2766 2771 + HgiCI* GGYRCC 2766 2770 . . 2766 2771 + Acc65I GGTACC 2766 2770 . . 2767 2770 + Csp6I GTAC 2767 2769 . . 2767 2770 + RsaI GTAC 2768 2768 . . 2769 2772 + SetI ASST 2772 2768 . . 2784 2789 + Hpy178III* TCNNGA 2785 2787 . . 2806 2810 + Hpy188I TCNGA 2808 2807 . . 2815 2819 + MaeIII GTNAC 2814 2819 . . 2828 2831 + CviRI* TGCA 2829 2829 . . 2833 2843 + FalI AAGNNNNNCTT 2824 2819 2856 2851 2835 2839 + MboII GAAGA 2847 2846 . . 2833 2843 - FalI AAGNNNNNCTT 2856 2851 2824 2819 2854 2858 + HphI GGTGA 2866 2865 . . 2857 2861 + MboII GAAGA 2869 2868 . . 2864 2873 + BsaBI GATNNNNATC 2868 2868 . . 2872 2876 - MboII GAAGA 2864 2863 . . 2877 2882 + VspI ATTAAT 2878 2880 . . 2874 2878 - TspDTI ATGAA 2864 2862 . . 2878 2881 + MseI TTAA 2878 2880 . . 2884 2887 + MseI TTAA 2884 2886 . . 2897 2901 + DdeI CTNAG 2897 2900 . . 2925 2928 + SetI ASST 2928 2924 . . 2925 2928 + CviJI RGCY 2926 2926 . . 2925 2928 + AluI AGCT 2926 2926 . . 2926 2930 + BlsI GCNGC 2928 2927 . . 2926 2930 + BisI GCNGC 2927 2928 . . 2926 2930 + TseI GCWGC 2926 2929 . . 2926 2930 - BbvI GCAGC 2913 2917 . . 2953 2958 + ApoI RAATTY 2953 2957 . . 2954 2957 + TspEI AATT 2953 2957 . . 2960 2970 + FalI AAGNNNNNCTT 2951 2946 2983 2978 2960 2970 - FalI AAGNNNNNCTT 2983 2978 2951 2946 2972 2977 + GsaI CCCAGC 2976 2972 . . 2972 2977 + BseYI CCCAGC 2972 2976 . . 2977 2981 + Hpy99I CGWCG 2981 2976 . . 2979 2982 + TaqI TCGA 2979 2981 . . 2976 2980 - HgaI GACGC 2965 2970 . . 2981 2985 + MboII GAAGA 2993 2992 . . 2984 2988 - BccI CCATC 2978 2979 . . 2989 2994 + VspI ATTAAT 2990 2992 . . 2990 2993 + MseI TTAA 2990 2992 . . 2992 2995 + TspEI AATT 2991 2995 . . 2998 3002 + Tsp4CI* ACNGT 3000 2999 . . 3001 3004 + Csp6I GTAC 3001 3003 . . 3001 3004 + RsaI GTAC 3002 3002 . . 3008 3011 + SetI ASST 3011 3007 . . 3016 3025 + XmnI GAANNNNTTC 3020 3020 . . 3017 3020 + TspEI AATT 3016 3020 . . 3029 3033 + Hpy188I TCNGA 3031 3030 . . 3032 3036 - BsmAI GTCTC 3026 3030 . . 3060 3063 + FatI CATG 3059 3063 . . 3060 3063 + NlaIII CATG 3063 3059 . . 3060 3063 + CviAII CATG 3060 3062 . . 3062 3065 + CviRI* TGCA 3063 3063 . . 3065 3068 + TspEI AATT 3064 3068 . . 3083 3086 + TspEI AATT 3082 3086 . . 3092 3095 + Csp6I GTAC 3092 3094 . . 3092 3095 + RsaI GTAC 3093 3093 . . 3100 3103 + Csp6I GTAC 3100 3102 . . 3100 3103 + RsaI GTAC 3101 3101 . . 3091 3101 - BaeI ACNNNNGTAYC 3116 3111 3083 3078 3105 3109 - BceAI ACGGC 3090 3092 . . 3099 3109 - BaeI ACNNNNGTAYC 3124 3119 3091 3086 3110 3115 + TsoI TARCCA 3126 3124 . . 3111 3114 + CviJI RGCY 3112 3112 . . 3159 3162 + SetI ASST 3162 3158 . . 3186 3190 + BsrI ACTGG 3191 3189 . . 3192 3195 + TspEI AATT 3191 3195 . . 3208 3212 + BssKI CCNGG 3207 3212 . . 3208 3212 + ScrFI CCNGG 3209 3210 . . 3208 3212 + CauII* CCSGG 3209 3210 . . 3209 3212 + HpaII CCGG 3209 3211 . . 3211 3214 + CviJI RGCY 3212 3212 . . 3214 3217 + MseI TTAA 3214 3216 . . 3216 3219 + TspEI AATT 3215 3219 . . 3224 3229 + PsiI TTATAA 3226 3226 . . 3239 3243 - BccI CCATC 3233 3234 . . 3246 3249 + Csp6I GTAC 3246 3248 . . 3246 3249 + RsaI GTAC 3247 3247 . . 3248 3251 + SetI ASST 3251 3247 . . 3257 3261 + FokI GGATG 3270 3274 . . 3257 3261 + BseGI GGATG 3263 3261 . . 3291 3296 + ApoI RAATTY 3291 3295 . . 3292 3295 + TspEI AATT 3291 3295 . . 3298 3301 + Csp6I GTAC 3298 3300 . . 3298 3301 + RsaI GTAC 3299 3299 . . 3303 3306 + SetI ASST 3306 3302 . . 3304 3308 + Hin4II* CCTTC 3314 3313 . . 3308 3313 + NdeI CATATG 3309 3311 . . 3306 3310 - TspDTI ATGAA 3296 3294 . . 3315 3318 + Csp6I GTAC 3315 3317 . . 3315 3318 + RsaI GTAC 3316 3316 . . 3317 3321 + BsrI ACTGG 3322 3320 . . 3322 3327 + ApoI RAATTY 3322 3326 . . 3323 3326 + TspEI AATT 3322 3326 . . 3328 3338 + Hin4I GAYNNNNNVTC 3319 3314 3351 3346 3338 3341 + FatI CATG 3337 3341 . . 3338 3341 + NlaIII CATG 3341 3337 . . 3338 3341 + CviAII CATG 3338 3340 . . 3343 3347 + HphI GGTGA 3355 3354 . . 3361 3364 - AciI CCGC 3361 3363 . . 3361 3365 - FauI CCCGC 3354 3356 . . 3366 3369 + SetI ASST 3369 3365 . . 3365 3368 - MnlI CCTC 3358 3357 . . 3392 3396 + TspDTI ATGAA 3407 3405 . . 3396 3399 + SetI ASST 3399 3395 . . 3397 3401 + HphI GGTGA 3409 3408 . . 3394 3398 - Hin4II* CCTTC 3388 3387 . . 3399 3410 + BdaI TGANNNNNNTCA 3388 3386 3422 3420 3400 3405 + ApoI RAATTY 3400 3404 . . 3401 3404 + TspEI AATT 3400 3404 . . 3405 3410 + BclI TGATCA 3405 3409 . . 3406 3409 + DpnI GATC 3407 3407 . . 3406 3409 + BstKTI GATC 3408 3406 . . 3406 3409 + MboI GATC 3405 3409 . . 3399 3410 - BdaI TGANNNNNNTCA 3422 3420 3388 3386 3419 3422 + MseI TTAA 3419 3421 . . 3426 3429 - MnlI CCTC 3419 3418 . . 3431 3434 + FatI CATG 3430 3434 . . 3431 3434 + NlaIII CATG 3434 3430 . . 3431 3434 + CviAII CATG 3431 3433 . . 3435 3438 - MnlI CCTC 3428 3427 . . 3439 3444 + ApoI RAATTY 3439 3443 . . 3440 3443 + TspEI AATT 3439 3443 . . 3451 3455 + MboII GAAGA 3463 3462 . . 3451 3460 + XmnI GAANNNNTTC 3455 3455 . . 3465 3469 + Hpy188I TCNGA 3467 3466 . . 3468 3472 - Hin4II* CCTTC 3462 3461 . . 3472 3475 - MnlI CCTC 3465 3464 . . 3479 3483 + Tsp45I GTSAC 3478 3483 . . 3479 3483 + MaeIII GTNAC 3478 3483 . . 3481 3485 + HinfI GANTC 3481 3484 . . 3481 3485 - PleI GAGTC 3475 3476 . . 3481 3485 - MlyI GAGTC 3475 3475 . . 3485 3489 + Hin4II* CCTTC 3495 3494 . . 3492 3495 + CviRI* TGCA 3493 3493 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 549 2949 # # Acc65I: # [GGTACC] # 732 2766 # # AccI: # [GTMKAC] # 539 783 2176 # # AciI: # [CCGC] # 24 79 82 126 137 175 # 180 1028 1667 # # AflIII: # [ACRYGT] # 1218 2280 # # AgeI: # [ACCGGT] # 229 3269 # # AhaIII*: # [TTTAAA] # 1057 1067 1374 # # AjuI: # [GAANNNNNNNTTGG] # 32 891 2575 # # AluI: # [AGCT] # 93 103 117 140 238 252 # 320 363 572 641 659 # # AlwNI: # [CAGNNNCTG] # 835 2663 # # ApaI: # [GGGCCC] # 824 2674 # # ApoI: # [RAATTY] # 31 39 59 78 102 178 # 295 330 338 385 500 525 # 638 # # AsuI*: # [GGNCC] # 1 128 692 1168 1509 # # AsuII: # [TTCGAA] # 263 294 428 2513 # # AvaI: # [CYCGRG] # 1407 2091 # # BaeI: # [ACNNNNGTAYC] # 8 8 25 33 374 549 # 2501 # # BalI: # [TGGCCA] # 1280 2218 # # BarI: # [GAAGNNNNNNTAC] # 32 32 50 749 2635 # # BbvI: # [GCAGC] # 456 585 2457 # # BbvII*: # [GAAGAC] # 398 819 827 1454 # # BccI: # [CCATC] # 13 43 59 80 175 228 # 229 252 255 265 567 622 # 710 # # BceAI: # [ACGGC] # 408 3090 # # BciVI: # [GTATCC] # 756 2742 # # BclI: # [TGATCA] # 93 3405 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 76 3388 # # BetI*: # [WCCGGW] # 229 549 2720 # # BglII: # [AGATCT] # 513 1158 1827 # # BinI*: # [GGATC] # 16 320 691 941 1530 # # BisI: # [GCNGC] # 127 139 179 571 594 1888 # # BlsI: # [GCNGC] # 128 139 179 570 594 1888 # # BmeT110I: # [CYCGRG] # 1406 2092 # # BmgT120I: # [GGNCC] # 1 129 692 1167 1509 # # Bpu10I: # [CCTNAGC] # 93 3405 # # BsaBI: # [GATNNNNATC] # 320 630 837 1711 # # BsaXI: # [ACNNNNNCTCC] # 30 30 282 1518 1638 # # BseBI: # [CCWGG] # 208 617 1031 1642 # # BseGI: # [GGATG] # 80 83 84 99 216 235 # 356 452 544 625 724 # # BseMII: # [CTCAG] # 107 189 1522 1680 # # BseRI: # [GAGGAG] # 1526 1972 # # BseSI: # [GKGCMC] # 284 540 2674 # # BseYI: # [CCCAGC] # 526 2972 # # BsiYI*: # [CCNNNNNNNGG] # 312 506 1037 1643 # # BslFI: # [GGGAC] # 544 662 1063 1229 # # BsmAI: # [GTCTC] # 68 442 472 2516 # # Bsp120I: # [GGGCCC] # 820 2678 # # Bsp1407I: # [TGTACA] # 629 1150 1719 # # BspCNI: # [CTCAG] # 106 189 1521 1682 # # BspMI: # [ACCTGC] # 549 2949 # # BsrI: # [ACTGG] # 131 176 1263 1928 # # BssKI: # [CCNGG] # 206 291 369 486 498 617 # 1031 # # BssNAI: # [GTATAC] # 784 2714 # # BstKTI: # [GATC] # 24 35 84 90 120 187 # 309 704 808 1137 # # BtgZI: # [GCGATG] # 1651 1847 # # BtsI: # [GCAGTG] # 1306 2192 # # Cac8I: # [GCNNGC] # 185 1419 1894 # # CauII*: # [CCSGG] # 289 369 498 2342 # # Cfr10I: # [RCCGGY] # 4 225 3269 # # CfrI: # [YGGCCR] # 303 1282 1913 # # Csp6I: # [GTAC] # 8 17 52 72 91 117 # 121 146 155 179 183 227 # 234 286 460 1150 # # CspCI: # [CAANNNNNGTGG] # 35 35 257 282 2889 # # CviAII: # [CATG] # 22 67 93 278 326 416 # 922 1374 # # CviJI: # [RGCY] # 6 8 10 10 11 13 # 15 18 20 24 24 30 # 32 38 71 71 79 84 # 88 93 100 102 104 108 # 114 123 140 158 186 202 # 202 252 286 313 363 # # CviRI*: # [TGCA] # 5 200 234 247 309 430 # 864 1209 # # DdeI: # [CTNAG] # 23 70 189 601 912 1703 # # DpnI: # [GATC] # 23 35 84 91 120 187 # 309 704 808 1137 # # DraII: # [RGGNCCY] # 821 2677 # # DrdI: # [GACNNNNNNGTC] # 1355 2143 # # Eam1105I: # [GACNNNNNGTC] # 85 3413 # # Eco31I: # [GGTCTC] # 914 2584 # # Eco57MI: # [CTGRAG] # 1402 2096 # # EcoNI: # [CCTNNNNNAGG] # 1645 1853 # # EcoRII: # [CCWGG] # 206 617 1031 1644 # # EcoRV: # [GATATC] # 405 1500 1593 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 32 32 32 32 # 95 515 797 979 984 # # FatI: # [CATG] # 22 68 93 278 326 416 # 921 1374 # # FauI: # [CCCGC] # 144 556 1131 1667 # # FnuDII*: # [CGCG] # 308 466 2724 # # FokI: # [GGATG] # 80 83 84 119 223 228 # 356 452 544 605 724 # # GlaI: # [GCGC] # 92 179 3227 # # GsaI: # [CCCAGC] # 522 2976 # # GsuI: # [CTGGAG] # 1402 2096 # # HaeIII: # [GGCC] # 114 129 176 517 652 744 # 1166 # # HgaI: # [GACGC] # 533 1060 1905 # # HgiAI*: # [GWGCWC] # 102 3396 # # HgiCI*: # [GGYRCC] # 537 732 2229 # # HgiJII*: # [GRGCYC] # 824 1268 1406 # # HhaI: # [GCGC] # 92 180 3226 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 2 7 11 19 21 25 # 25 30 32 32 32 32 # 32 46 147 241 303 378 # 649 709 725 # # Hin4II*: # [CCTTC] # 3 25 33 74 74 197 # 348 2744 # # Hin6I: # [GCGC] # 92 178 3228 # # HindII: # [GTYRAC] # 1191 2307 # # HindIII: # [AAGCTT] # 826 2672 # # HinfI: # [GANTC] # 6 7 17 56 61 64 # 71 111 140 167 209 234 # 262 336 355 601 801 # # HpaII: # [CCGG] # 5 225 289 368 499 549 # 1563 # # HphI: # [GGTGA] # 54 80 89 196 228 267 # 460 489 732 903 # # Hpy166II: # [GTNNAC] # 105 132 144 183 229 237 # 302 364 1802 # # Hpy178III*: # [TCNNGA] # 18 21 25 25 27 47 # 59 86 118 145 213 261 # 319 411 500 510 713 # # Hpy188I: # [TCNGA] # 24 31 41 54 112 216 # 223 376 392 436 521 522 # 550 # # Hpy99I: # [CGWCG] # 192 257 517 551 890 1091 # # KpnI: # [GGTACC] # 728 2770 # # Ksp632I*: # [CTCTTC] # 53 132 869 934 1510 # # MaeI: # [CTAG] # 233 324 1118 1823 # # MaeII: # [ACGT] # 42 92 112 170 242 529 # 1093 1218 # # MaeIII: # [GTNAC] # 20 75 117 174 199 278 # 664 874 1097 # # MboI: # [GATC] # 21 35 84 93 120 187 # 309 704 808 1137 # # MboII: # [GAAGA] # 5 17 35 39 47 52 # 70 72 77 80 124 168 # 308 333 460 467 470 674 # # MfeI: # [CAATTG] # 125 1076 2297 # # MlyI: # [GAGTC] # 23 70 127 401 566 1063 # 1248 # # MmeI: # [TCCRAC] # 235 257 919 2087 # # MnlI: # [CCTC] # 9 9 9 9 22 27 # 33 37 50 61 113 113 # 126 127 138 355 579 667 # 1014 # # MseI: # [TTAA] # 4 6 25 47 78 79 # 81 93 96 102 106 107 # 127 141 205 224 224 241 # 252 275 318 320 347 # # MslI: # [CAYNNNNRTG] # 83 209 675 717 1814 # # MstI*: # [TGCGCA] # 179 3319 # # MwoI: # [GCNNNNNNNGC] # 9 83 186 368 890 1962 # # NdeI: # [CATATG] # 189 769 2540 # # NlaIII: # [CATG] # 22 64 93 278 326 416 # 925 1374 # # NlaIV: # [GGNNCC] # 264 275 283 386 437 730 # 1123 # # OliI: # [CACNNNNGTG] # 1601 1897 # # PacI: # [TTAATTAA] # 1414 2084 # # PleI: # [GAGTC] # 23 69 128 401 565 1063 # 1249 # # PsiI: # [TTATAA] # 244 272 892 2090 # # PstI: # [CTGCAG] # 1176 2322 # # RsaI: # [GTAC] # 8 17 52 72 91 117 # 121 146 155 179 182 228 # 234 286 460 1150 # # SapI: # [GCTCTTC] # 987 2511 # # ScaI: # [AGTACT] # 851 2647 # # ScrFI: # [CCNGG] # 208 289 369 486 498 617 # 1031 # # SduI: # [GDGCHC] # 102 284 438 1268 1406 # # SecI*: # [CCNNGG] # 823 2675 # # SetI: # [ASST] # 11 12 15 21 24 29 # 29 30 42 44 45 47 # 49 55 55 62 63 65 # 68 72 75 79 81 83 # 83 89 92 93 94 95 # 96 99 104 111 113 146 # 151 156 204 238 378 # # SfaNI: # [GCATC] # 12 24 150 265 293 557 # 831 1366 # # SfeI*: # [CTRYAG] # 882 1180 1436 # # SspI: # [AATATT] # 927 1050 1521 # # TaiI: # [ACGT] # 42 92 112 170 242 532 # 1093 1215 # # TaqI: # [TCGA] # 57 66 87 136 260 263 # 294 294 428 519 1094 # # TatI: # [WGTACW] # 117 512 853 866 1150 # # TauI: # [GCSGC] # 129 179 1163 2027 # # TfiI: # [GAWTC] # 13 71 111 167 201 209 # 298 318 601 691 818 # # TseI: # [GCWGC] # 444 572 2482 # # TsoI: # [TARCCA] # 202 372 379 2545 # # Tsp45I: # [GTSAC] # 20 1333 2145 # # Tsp4CI*: # [ACNGT] # 25 36 67 85 102 122 # 135 161 272 432 498 510 # 1053 # # TspDTI: # [ATGAA] # 40 61 71 78 91 111 # 112 121 138 143 164 168 # 249 257 305 432 441 516 # # TspEI: # [AATT] # 5 6 7 10 18 20 # 21 24 25 26 30 31 # 38 38 39 39 48 53 # 53 59 66 72 72 76 # 78 99 102 109 113 125 # 149 177 202 219 239 295 # 323 392 # # TspGWI: # [ACGGA] # 23 328 1066 2081 # # TspRI: # [CASTGNN] # 90 200 264 531 1107 1306 # # TstI: # [CACNNNNNNTCC] # 32 32 553 1437 1444 # # Tth111I: # [GACNNNGTC] # 1355 2143 # # VspI: # [ATTAAT] # 112 508 2878 # # XbaI: # [TCTAGA] # 557 1117 1824 # # XhoII: # [RGATCY] # 120 393 891 936 1158 # # XmnI: # [GAANNNNTTC] # 43 435 3020 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3498 # Reported_sequences: 1 # Reported_hitcount: 754 #---------------------------------------