Restriction Fragments for GRX7/YBR014C



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The currently selected gene/sequence is :

GRX7/YBR014C on chromosome II from coordinates 267336 to 266725.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28094.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + CviJI RGCY 4 4 . . 11 16 + PsiI TTATAA 13 13 . . 26 29 + TaiI ACGT 29 25 . . 26 29 + SetI ASST 29 25 . . 26 29 + MaeII ACGT 26 28 . . 32 36 + PleI GAGTC 40 41 . . 32 36 + MlyI GAGTC 41 41 . . 32 36 + HinfI GANTC 32 35 . . 49 52 + TspEI AATT 48 52 . . 60 69 + MslI CAYNNNNRTG 64 64 . . 79 82 + MseI TTAA 79 81 . . 84 89 + EcoRI GAATTC 84 88 . . 84 89 + ApoI RAATTY 84 88 . . 85 88 + TspEI AATT 84 88 . . 87 92 + AsuII TTCGAA 88 90 . . 88 91 + TaqI TCGA 88 90 . . 90 95 + BsmI GAATGC 96 94 . . 94 99 + Cac8I GCNNGC 96 96 . . 94 99 + BmtI GCTAGC 98 94 . . 94 99 + NheI GCTAGC 94 98 . . 95 98 + MaeI CTAG 95 97 . . 100 105 + Hpy166II GTNNAC 102 102 . . 100 105 + HindII GTYRAC 102 102 . . 98 102 - HgaI GACGC 87 92 . . 123 127 + BccI CCATC 131 132 . . 120 124 - HphI GGTGA 112 111 . . 126 131 + Hpy178III* TCNNGA 127 129 . . 124 128 - FokI GGATG 110 114 . . 124 128 - BseGI GGATG 123 121 . . 130 134 + TfiI GAWTC 130 133 . . 130 134 + HinfI GANTC 130 133 . . 132 136 - TspDTI ATGAA 122 120 . . 138 142 + HphI GGTGA 150 149 . . 139 143 + Tsp45I GTSAC 138 143 . . 139 143 + MaeIII GTNAC 138 143 . . 142 145 + TaiI ACGT 145 141 . . 142 145 + SetI ASST 145 141 . . 142 145 + MaeII ACGT 142 144 . . 151 154 + TspEI AATT 150 154 . . 154 159 + Hpy178III* TCNNGA 155 157 . . 162 165 + CviRI* TGCA 163 163 . . 165 168 + SetI ASST 168 164 . . 166 170 + BssKI CCNGG 165 170 . . 166 170 + BseBI CCWGG 167 168 . . 166 170 + ScrFI CCNGG 167 168 . . 166 170 + EcoRII CCWGG 165 170 . . 174 178 - HphI GGTGA 166 165 . . 187 190 + FatI CATG 186 190 . . 187 190 + NlaIII CATG 190 186 . . 187 190 + CviAII CATG 187 189 . . 193 197 + Tsp4CI* ACNGT 195 194 . . 216 219 + CviJI RGCY 217 217 . . 220 224 + MboII GAAGA 232 231 . . 242 246 + BslFI GGGAC 256 260 . . 244 248 + HgaI GACGC 253 258 . . 250 255 + ApoI RAATTY 250 254 . . 251 254 + TspEI AATT 250 254 . . 259 263 + TauI GCSGC 262 259 . . 259 263 + BlsI GCNGC 261 260 . . 259 263 + BisI GCNGC 260 261 . . 260 265 + NspBII* CMGCKG 262 262 . . 256 260 - SfaNI GCATC 246 250 . . 260 263 + AciI CCGC 260 262 . . 262 266 + BlsI GCNGC 264 263 . . 262 266 + BisI GCNGC 263 264 . . 262 266 + TseI GCWGC 262 265 . . 263 268 + PstI CTGCAG 267 263 . . 263 268 + SfeI* CTRYAG 263 267 . . 264 267 + CviRI* TGCA 265 265 . . 262 266 - BbvI GCAGC 249 253 . . 288 292 + Tsp4CI* ACNGT 290 289 . . 291 295 + Tsp45I GTSAC 290 295 . . 291 295 + MaeIII GTNAC 290 295 . . 293 298 + TaqII CACCCA 309 307 . . 292 296 - HphI GGTGA 284 283 . . 297 300 + FatI CATG 296 300 . . 297 300 + NlaIII CATG 300 296 . . 297 300 + CviAII CATG 297 299 . . 311 321 + MwoI GCNNNNNNNGC 317 314 . . 316 320 + BsrI ACTGG 321 319 . . 319 322 + CviJI RGCY 320 320 . . 320 324 + BlsI GCNGC 322 321 . . 320 324 + BisI GCNGC 321 322 . . 320 324 + TseI GCWGC 320 323 . . 323 333 + MwoI GCNNNNNNNGC 329 326 . . 320 324 - BbvI GCAGC 307 311 . . 344 349 + HindIII AAGCTT 344 348 . . 345 348 + SetI ASST 348 344 . . 345 348 + CviJI RGCY 346 346 . . 345 348 + AluI AGCT 346 346 . . 351 356 + Hpy166II GTNNAC 353 353 . . 351 356 + HindII GTYRAC 353 353 . . 358 361 + TspEI AATT 357 361 . . 363 368 + Hpy166II GTNNAC 365 365 . . 363 366 + Csp6I GTAC 363 365 . . 363 366 + RsaI GTAC 364 364 . . 365 370 + AflIII ACRYGT 365 369 . . 366 377 + TstI CACNNNNNNTCC 357 352 389 384 367 370 + TaiI ACGT 370 366 . . 367 370 + SetI ASST 370 366 . . 367 370 + MaeII ACGT 367 369 . . 367 377 + BsaXI ACNNNNNCTCC 357 354 387 384 376 380 + BccI CCATC 384 385 . . 386 389 + TaiI ACGT 389 385 . . 386 389 + SetI ASST 389 385 . . 386 389 + MaeII ACGT 386 388 . . 395 398 + TspEI AATT 394 398 . . 445 449 + Tsp45I GTSAC 444 449 . . 445 449 + MaeIII GTNAC 444 449 . . 447 453 + TspRI CASTGNN 453 444 . . 448 452 + BsrI ACTGG 453 451 . . 460 464 + Tsp4CI* ACNGT 462 461 . . 463 467 - BslFI GGGAC 448 452 . . 469 474 + AclI AACGTT 470 472 . . 470 473 + TaiI ACGT 473 469 . . 470 473 + SetI ASST 473 469 . . 470 473 + MaeII ACGT 470 472 . . 485 488 + Csp6I GTAC 485 487 . . 485 488 + RsaI GTAC 486 486 . . 490 495 + Hpy178III* TCNNGA 491 493 . . 495 498 + SetI ASST 498 494 . . 494 497 - MnlI CCTC 487 486 . . 498 503 - TsoI TARCCA 488 486 . . 506 510 + DdeI CTNAG 506 509 . . 506 510 - BspCNI CTCAG 498 496 . . 506 510 - BseMII CTCAG 497 495 . . 519 523 + MaeIII GTNAC 518 523 . . 523 532 + MslI CAYNNNNRTG 527 527 . . 533 537 + TspDTI ATGAA 548 546 . . 540 545 + XbaI TCTAGA 540 544 . . 540 545 + Hpy178III* TCNNGA 541 543 . . 541 544 + MaeI CTAG 541 543 . . 544 548 + HinfI GANTC 544 547 . . 544 548 - PleI GAGTC 538 539 . . 544 548 - MlyI GAGTC 538 538 . . 563 568 + BtgZI GCGATG 578 582 . . 565 569 - BccI CCATC 559 560 . . 573 578 + Hpy166II GTNNAC 575 575 . . 577 581 + Tsp4CI* ACNGT 579 578 . . 585 588 + SetI ASST 588 584 . . 585 588 + CviJI RGCY 586 586 . . 585 588 + AluI AGCT 586 586 . . 589 592 + TspEI AATT 588 592 . . 598 602 + Hpy188I TCNGA 600 599 . . 599 603 + DdeI CTNAG 599 602 . . 599 603 - BspCNI CTCAG 591 589 . . 599 603 - BseMII CTCAG 590 588 . . 609 612 + MaeI CTAG 609 611 . . #--------------------------------------- # # # AciI: # [CCGC] # 260 352 # # AclI: # [AACGTT] # 142 470 # # AflIII: # [ACRYGT] # 247 365 # # AluI: # [AGCT] # 26 240 346 # # ApoI: # [RAATTY] # 84 166 362 # # AsuII: # [TTCGAA] # 88 524 # # BbvI: # [GCAGC] # 58 249 305 # # BccI: # [CCATC] # 53 131 175 253 # # BisI: # [GCNGC] # 3 58 260 291 # # BlsI: # [GCNGC] # 3 58 261 290 # # BmtI: # [GCTAGC] # 98 514 # # BsaXI: # [ACNNNNNCTCC] # 30 225 357 # # BseBI: # [CCWGG] # 167 445 # # BseGI: # [GGATG] # 123 489 # # BseMII: # [CTCAG] # 22 93 497 # # BslFI: # [GGGAC] # 164 192 256 # # BsmI: # [GAATGC] # 96 516 # # BspCNI: # [CTCAG] # 21 93 498 # # BsrI: # [ACTGG] # 132 159 321 # # BssKI: # [CCNGG] # 165 447 # # BtgZI: # [GCGATG] # 34 578 # # Cac8I: # [GCNNGC] # 96 516 # # Csp6I: # [GTAC] # 122 127 363 # # CviAII: # [CATG] # 110 187 315 # # CviJI: # [RGCY] # 4 26 26 103 213 240 # # CviRI*: # [TGCA] # 102 163 347 # # DdeI: # [CTNAG] # 13 93 506 # # EcoRI: # [GAATTC] # 84 528 # # EcoRII: # [CCWGG] # 165 447 # # FatI: # [CATG] # 110 186 316 # # FokI: # [GGATG] # 110 502 # # HgaI: # [GACGC] # 87 166 359 # # HindII: # [GTYRAC] # 102 251 259 # # HindIII: # [AAGCTT] # 268 344 # # HinfI: # [GANTC] # 32 68 98 414 # # HphI: # [GGTGA] # 16 38 112 118 328 # # Hpy166II: # [GTNNAC] # 12 37 102 210 251 # # Hpy178III*: # [TCNNGA] # 28 50 71 127 336 # # Hpy188I: # [TCNGA] # 12 600 # # MaeI: # [CTAG] # 3 68 95 446 # # MaeII: # [ACGT] # 19 26 84 116 142 225 # # MaeIII: # [GTNAC] # 74 94 138 152 154 # # MboII: # [GAAGA] # 232 380 # # MlyI: # [GAGTC] # 41 74 497 # # MnlI: # [CCTC] # 125 487 # # MseI: # [TTAA] # 79 533 # # MslI: # [CAYNNNNRTG] # 64 85 463 # # MwoI: # [GCNNNNNNNGC] # 12 283 317 # # NheI: # [GCTAGC] # 94 518 # # NlaIII: # [CATG] # 110 190 312 # # NspBII*: # [CMGCKG] # 262 350 # # PleI: # [GAGTC] # 40 74 498 # # PsiI: # [TTATAA] # 13 599 # # PstI: # [CTGCAG] # 267 345 # # RsaI: # [GTAC] # 122 126 364 # # ScrFI: # [CCNGG] # 167 445 # # SetI: # [ASST] # 19 22 23 24 25 29 # 84 90 116 180 # # SfaNI: # [GCATC] # 246 366 # # SfeI*: # [CTRYAG] # 263 349 # # TaiI: # [ACGT] # 19 29 84 116 139 225 # # TaqI: # [TCGA] # 88 524 # # TaqII: # [CACCCA] # 303 309 # # TauI: # [GCSGC] # 262 350 # # TfiI: # [GAWTC] # 130 482 # # TseI: # [GCWGC] # 58 262 292 # # TsoI: # [TARCCA] # 124 488 # # Tsp45I: # [GTSAC] # 138 152 154 168 # # Tsp4CI*: # [ACNGT] # 33 95 117 172 195 # # TspDTI: # [ATGAA] # 64 122 426 # # TspEI: # [AATT] # 24 36 37 48 66 100 # 107 194 # # TspRI: # [CASTGNN] # 159 453 # # TstI: # [CACNNNNNNTCC] # 32 223 357 # # XbaI: # [TCTAGA] # 72 540 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 612 # Reported_sequences: 1 # Reported_hitcount: 148 #---------------------------------------