Restriction Fragments for YBL113W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YBL113W-A on chromosome II from coordinates 646 to 1128.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.31876.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + Cac8I GCNNGC 8 8 . . 16 21 + PstI CTGCAG 20 16 . . 16 21 + SfeI* CTRYAG 16 20 . . 17 20 + CviRI* TGCA 18 18 . . 18 22 + BlsI GCNGC 20 19 . . 18 22 + BisI GCNGC 19 20 . . 18 22 + BbvI GCAGC 30 34 . . 18 22 + TseI GCWGC 18 21 . . 20 23 + SetI ASST 23 19 . . 20 23 + CviJI RGCY 21 21 . . 20 23 + AluI AGCT 21 21 . . 26 29 + TaiI ACGT 29 25 . . 26 29 + SetI ASST 29 25 . . 26 29 + MaeII ACGT 26 28 . . 28 31 + Csp6I GTAC 28 30 . . 28 31 + RsaI GTAC 29 29 . . 33 43 + MwoI GCNNNNNNNGC 39 36 . . 35 40 + NlaIV GGNNCC 37 37 . . 35 39 + BmgT120I GGNCC 36 37 . . 35 39 + AvaII GGWCC 35 38 . . 35 39 + AsuI* GGNCC 35 38 . . 33 36 - AciI CCGC 33 35 . . 36 47 + DrdI GACNNNNNNGTC 42 40 . . 40 45 + PstI CTGCAG 44 40 . . 40 45 + SfeI* CTRYAG 40 44 . . 41 44 + CviRI* TGCA 42 42 . . 49 54 + HaeII RGCGCY 53 49 . . 49 54 + Eco47III AGCGCT 51 51 . . 50 53 + Hin6I GCGC 50 52 . . 50 53 + GlaI GCGC 51 51 . . 50 53 + HhaI GCGC 52 50 . . 58 61 + FatI CATG 57 61 . . 58 61 + NlaIII CATG 61 57 . . 58 61 + CviAII CATG 58 60 . . 82 87 + MmeI TCCRAC 107 105 . . 90 95 + Hpy178III* TCNNGA 91 93 . . 91 96 + SmlI CTYRAG 91 95 . . 91 96 + XhoI CTCGAG 91 95 . . 91 96 + BmeT110I CYCGRG 92 94 . . 91 96 + AvaI CYCGRG 91 95 . . 92 95 + TaqI TCGA 92 94 . . 95 100 + HaeII RGCGCY 99 95 . . 95 100 + Eco47III AGCGCT 97 97 . . 96 99 + Hin6I GCGC 96 98 . . 96 99 + GlaI GCGC 97 97 . . 96 99 + HhaI GCGC 98 96 . . 109 120 + DrdI GACNNNNNNGTC 115 113 . . 110 114 + Tsp4CI* ACNGT 112 111 . . 126 129 + MnlI CCTC 136 135 . . 134 138 - MboII GAAGA 126 125 . . 138 142 + BssKI CCNGG 137 142 . . 138 142 + ScrFI CCNGG 139 140 . . 138 142 + CauII* CCSGG 139 140 . . 139 142 + HpaII CCGG 139 141 . . 154 158 - BslFI GGGAC 139 143 . . 157 161 - BsrI ACTGG 157 155 . . 156 161 - BfiI ACTGGG 151 150 . . 168 173 + TatI WGTACW 168 172 . . 168 173 + ScaI AGTACT 170 170 . . 169 172 + Csp6I GTAC 169 171 . . 169 172 + RsaI GTAC 170 170 . . 172 177 + PstI CTGCAG 176 172 . . 172 177 + SfeI* CTRYAG 172 176 . . 173 176 + CviRI* TGCA 174 174 . . 174 178 + BlsI GCNGC 176 175 . . 174 178 + BisI GCNGC 175 176 . . 174 178 + BbvI GCAGC 186 190 . . 174 178 + TseI GCWGC 174 177 . . 179 182 + SetI ASST 182 178 . . 180 183 + MnlI CCTC 190 189 . . 189 192 + TaqI TCGA 189 191 . . 186 190 - MboII GAAGA 178 177 . . 185 190 - BbvII* GAAGAC 178 182 . . 191 195 + TfiI GAWTC 191 194 . . 191 195 + HinfI GANTC 191 194 . . 215 220 + PsiI TTATAA 217 217 . . 232 235 + DpnI GATC 233 233 . . 232 235 + BstKTI GATC 234 232 . . 232 235 + MboI GATC 231 235 . . 238 242 + Hin4II* CCTTC 248 247 . . 241 246 + Hpy178III* TCNNGA 242 244 . . 246 251 + NspI RCATGY 250 246 . . 247 250 + FatI CATG 246 250 . . 247 250 + NlaIII CATG 250 246 . . 247 250 + CviAII CATG 247 249 . . 250 253 - AciI CCGC 250 252 . . 261 264 + CviJI RGCY 262 262 . . 261 266 + NlaIV GGNNCC 263 263 . . 270 275 + Cac8I GCNNGC 272 272 . . 275 278 + MnlI CCTC 285 284 . . 282 285 + TaqI TCGA 282 284 . . 284 288 + TfiI GAWTC 284 287 . . 284 288 + HinfI GANTC 284 287 . . 298 301 + TspEI AATT 297 301 . . 306 311 + Ksp632I* CTCTTC 312 315 . . 307 311 - MboII GAAGA 299 298 . . 310 314 - MboII GAAGA 302 301 . . 314 317 + MaeI CTAG 314 316 . . 321 326 + BalI TGGCCA 323 323 . . 321 326 + CfrI YGGCCR 321 325 . . 322 325 + CviJI RGCY 323 323 . . 322 325 + HaeIII GGCC 323 323 . . 324 328 + BssKI CCNGG 323 328 . . 324 328 + BseBI CCWGG 325 326 . . 324 328 + ScrFI CCNGG 325 326 . . 324 328 + EcoRII CCWGG 323 328 . . 326 329 + SetI ASST 329 325 . . 327 332 + KpnI GGTACC 331 327 . . 327 332 + NlaIV GGNNCC 329 329 . . 327 332 + HgiCI* GGYRCC 327 331 . . 327 332 + Acc65I GGTACC 327 331 . . 328 331 + Csp6I GTAC 328 330 . . 328 331 + RsaI GTAC 329 329 . . 339 342 + TspEI AATT 338 342 . . 331 344 - AjuI GAANNNNNNNTTGG 356 351 324 319 351 355 + Hpy188I TCNGA 353 352 . . 352 356 + DdeI CTNAG 352 355 . . 352 356 - BspCNI CTCAG 344 342 . . 352 356 - BseMII CTCAG 343 341 . . 356 366 + BplI GAGNNNNNCTC 347 342 379 374 360 365 + XhoII RGATCY 360 364 . . 360 365 + BglII AGATCT 360 364 . . 361 364 + DpnI GATC 362 362 . . 361 364 + BstKTI GATC 363 361 . . 361 364 + MboI GATC 360 364 . . 354 364 - Hin4I GAYNNNNNVTC 377 372 345 340 356 366 - BplI GAGNNNNNCTC 379 374 347 342 384 389 + SspI AATATT 386 386 . . 399 404 + SfeI* CTRYAG 399 403 . . 404 410 + SanDI GGGWCCC 405 408 . . 404 410 + PpuMI RGGWCCY 405 408 . . 404 410 + DraII RGGNCCY 405 408 . . 404 409 + NlaIV GGNNCC 406 406 . . 405 410 + NlaIV GGNNCC 407 407 . . 405 409 + BmgT120I GGNCC 406 407 . . 405 409 + AvaII GGWCC 405 408 . . 405 409 + AsuI* GGNCC 405 408 . . 406 410 - BslFI GGGAC 391 395 . . 410 420 + BsiYI* CCNNNNNNNGG 416 413 . . 410 420 + PflMI CCANNNNNTGG 416 413 . . 411 414 + FatI CATG 410 414 . . 411 414 + NlaIII CATG 414 410 . . 411 414 + CviAII CATG 411 413 . . 419 422 + CviJI RGCY 420 420 . . 422 425 + TaqI TCGA 422 424 . . 428 432 - MboII GAAGA 420 419 . . 427 432 - BbvII* GAAGAC 420 424 . . 450 453 + MnlI CCTC 460 459 . . 462 466 + BlsI GCNGC 464 463 . . 462 466 + BisI GCNGC 463 464 . . 462 466 + BbvI GCAGC 474 478 . . 462 466 + TseI GCWGC 462 465 . . 465 470 - BsmI GAATGC 465 463 . . 478 481 + SetI ASST 481 477 . . 478 481 + CviJI RGCY 479 479 . . 478 481 + AluI AGCT 479 479 . . 480 483 + MaeI CTAG 480 482 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 156 327 # # AciI: # [CCGC] # 33 217 233 # # AjuI: # [GAANNNNNNNTTGG] # 32 127 324 # # AluI: # [AGCT] # 4 21 458 # # AsuI*: # [GGNCC] # 35 78 370 # # AvaI: # [CYCGRG] # 91 392 # # AvaII: # [GGWCC] # 35 78 370 # # BalI: # [TGGCCA] # 160 323 # # BbvI: # [GCAGC] # 9 30 156 288 # # BbvII*: # [GAAGAC] # 63 178 242 # # BfiI: # [ACTGGG] # 151 332 # # BglII: # [AGATCT] # 123 360 # # BisI: # [GCNGC] # 19 20 156 288 # # BlsI: # [GCNGC] # 19 20 156 288 # # BmeT110I: # [CYCGRG] # 92 391 # # BmgT120I: # [GGNCC] # 36 77 370 # # BplI: # [GAGNNNNNCTC] # 0 0 32 104 347 # # BseBI: # [CCWGG] # 158 325 # # BseMII: # [CTCAG] # 140 343 # # BsiYI*: # [CCNNNNNNNGG] # 67 416 # # BslFI: # [GGGAC] # 92 139 252 # # BsmI: # [GAATGC] # 18 465 # # BspCNI: # [CTCAG] # 139 344 # # BsrI: # [ACTGG] # 157 326 # # BssKI: # [CCNGG] # 137 160 186 # # BstKTI: # [GATC] # 120 129 234 # # Cac8I: # [GCNNGC] # 8 211 264 # # CauII*: # [CCSGG] # 139 344 # # CfrI: # [YGGCCR] # 162 321 # # Csp6I: # [GTAC] # 28 141 155 159 # # CviAII: # [CATG] # 58 72 164 189 # # CviJI: # [RGCY] # 4 21 59 61 97 241 # # CviRI*: # [TGCA] # 18 24 132 309 # # DdeI: # [CTNAG] # 131 352 # # DpnI: # [GATC] # 121 129 233 # # DraII: # [RGGNCCY] # 78 405 # # DrdI: # [GACNNNNNNGTC] # 42 73 368 # # Eco47III: # [AGCGCT] # 46 51 386 # # EcoRII: # [CCWGG] # 160 323 # # FatI: # [CATG] # 57 73 164 189 # # GlaI: # [GCGC] # 46 51 386 # # HaeII: # [RGCGCY] # 46 53 384 # # HaeIII: # [GGCC] # 160 323 # # HgiCI*: # [GGYRCC] # 156 327 # # HhaI: # [GCGC] # 46 52 385 # # Hin4I: # [GAYNNNNNVTC] # 32 106 345 # # Hin4II*: # [CCTTC] # 235 248 # # Hin6I: # [GCGC] # 46 50 387 # # HinfI: # [GANTC] # 93 191 199 # # HpaII: # [CCGG] # 139 344 # # Hpy178III*: # [TCNNGA] # 91 151 241 # # Hpy188I: # [TCNGA] # 130 353 # # KpnI: # [GGTACC] # 152 331 # # Ksp632I*: # [CTCTTC] # 171 312 # # MaeI: # [CTAG] # 3 166 314 # # MaeII: # [ACGT] # 26 457 # # MboI: # [GATC] # 123 129 231 # # MboII: # [GAAGA] # 3 52 63 118 121 126 # # MmeI: # [TCCRAC] # 107 376 # # MnlI: # [CCTC] # 23 54 95 136 175 # # MwoI: # [GCNNNNNNNGC] # 39 444 # # NlaIII: # [CATG] # 61 69 164 189 # # NlaIV: # [GGNNCC] # 1 37 66 76 77 226 # # NspI: # [RCATGY] # 233 250 # # PflMI: # [CCANNNNNTGG] # 67 416 # # PpuMI: # [RGGWCCY] # 78 405 # # PsiI: # [TTATAA] # 217 266 # # PstI: # [CTGCAG] # 20 24 132 307 # # RsaI: # [GTAC] # 29 141 154 159 # # SanDI: # [GGGWCCC] # 78 405 # # ScaI: # [AGTACT] # 170 313 # # ScrFI: # [CCNGG] # 139 158 186 # # SetI: # [ASST] # 2 6 23 147 152 153 # # SfeI*: # [CTRYAG] # 16 24 84 132 227 # # SmlI: # [CTYRAG] # 91 392 # # SspI: # [AATATT] # 97 386 # # TaiI: # [ACGT] # 29 454 # # TaqI: # [TCGA] # 61 92 93 97 140 # # TatI: # [WGTACW] # 168 315 # # TfiI: # [GAWTC] # 93 191 199 # # TseI: # [GCWGC] # 18 21 156 288 # # Tsp4CI*: # [ACNGT] # 112 371 # # TspEI: # [AATT] # 41 145 297 # # XhoI: # [CTCGAG] # 91 392 # # XhoII: # [RGATCY] # 123 360 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 483 # Reported_sequences: 1 # Reported_hitcount: 157 #---------------------------------------