Restriction Fragments for YBL100W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YBL100W-A on chromosome II from coordinates 29935 to 31251.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.11200.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TfiI GAWTC 4 7 . . 4 8 + HinfI GANTC 4 7 . . 14 17 + TspEI AATT 13 17 . . 40 43 + FatI CATG 39 43 . . 40 43 + NlaIII CATG 43 39 . . 40 43 + CviAII CATG 40 42 . . 46 51 + HaeII RGCGCY 50 46 . . 47 50 + Hin6I GCGC 47 49 . . 47 50 + GlaI GCGC 48 48 . . 47 50 + HhaI GCGC 49 47 . . 61 65 + MaeIII GTNAC 60 65 . . 68 72 + DdeI CTNAG 68 71 . . 76 80 + BsmAI GTCTC 81 85 . . 87 92 + Hpy178III* TCNNGA 88 90 . . 90 95 + XhoII RGATCY 90 94 . . 91 94 + DpnI GATC 92 92 . . 91 94 + BstKTI GATC 93 91 . . 91 94 + MboI GATC 90 94 . . 91 95 - BinI* GGATC 85 86 . . 93 97 - TspGWI ACGGA 83 81 . . 99 102 + CviJI RGCY 100 100 . . 100 110 + MwoI GCNNNNNNNGC 106 103 . . 100 104 - BceAI ACGGC 85 87 . . 108 111 + SetI ASST 111 107 . . 108 111 + CviJI RGCY 109 109 . . 108 111 + AluI AGCT 109 109 . . 115 118 + TspEI AATT 114 118 . . 120 125 + BetI* WCCGGW 120 124 . . 121 124 + HpaII CCGG 121 123 . . 124 129 + ApoI RAATTY 124 128 . . 125 128 + TspEI AATT 124 128 . . 136 140 + TfiI GAWTC 136 139 . . 136 140 + HinfI GANTC 136 139 . . 143 147 + DdeI CTNAG 143 146 . . 146 149 + SetI ASST 149 145 . . 149 152 + MseI TTAA 149 151 . . 151 154 + TspEI AATT 150 154 . . 163 167 - BsmAI GTCTC 157 161 . . 171 174 + SetI ASST 174 170 . . 172 176 + BssKI CCNGG 171 176 . . 172 176 + BseBI CCWGG 173 174 . . 172 177 + SecI* CCNNGG 172 176 . . 172 176 + ScrFI CCNGG 173 174 . . 172 176 + EcoRII CCWGG 171 176 . . 175 179 + BslFI GGGAC 189 193 . . 182 187 + PvuII CAGCTG 184 184 . . 182 187 + NspBII* CMGCKG 184 184 . . 183 186 + SetI ASST 186 182 . . 183 186 + CviJI RGCY 184 184 . . 183 186 + AluI AGCT 184 184 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 198 202 + BccI CCATC 206 207 . . 199 208 + MslI CAYNNNNRTG 203 203 . . 205 208 + FatI CATG 204 208 . . 205 208 + NlaIII CATG 208 204 . . 205 208 + CviAII CATG 205 207 . . 208 212 + BsmAI GTCTC 213 217 . . 214 217 + MnlI CCTC 224 223 . . 212 217 - BseRI GAGGAG 203 201 . . 219 222 + SetI ASST 222 218 . . 219 224 + BspMI ACCTGC 228 232 . . 224 229 - Eco57MI CTGRAG 209 207 . . 229 232 + Csp6I GTAC 229 231 . . 229 232 + RsaI GTAC 230 230 . . 224 229 - Eco57I CTGAAG 209 207 . . 234 237 + SetI ASST 237 233 . . 235 238 + MnlI CCTC 245 244 . . 238 248 + BsiYI* CCNNNNNNNGG 244 241 . . 238 248 + PflMI CCANNNNNTGG 244 241 . . 249 253 + Tsp4CI* ACNGT 251 250 . . 252 255 + Csp6I GTAC 252 254 . . 252 255 + RsaI GTAC 253 253 . . 263 267 + BceAI ACGGC 279 281 . . 267 270 + FatI CATG 266 270 . . 267 270 + NlaIII CATG 270 266 . . 267 270 + CviAII CATG 267 269 . . 273 277 + HinfI GANTC 273 276 . . 273 277 - PleI GAGTC 267 268 . . 273 277 - MlyI GAGTC 267 267 . . 285 288 + SetI ASST 288 284 . . 285 288 + CviJI RGCY 286 286 . . 285 288 + AluI AGCT 286 286 . . 291 294 + CviJI RGCY 292 292 . . 299 303 + BsrI ACTGG 304 302 . . 299 304 + BfiI ACTGGG 309 308 . . 302 307 + SduI GDGCHC 306 302 . . 302 307 + BseSI GKGCMC 306 302 . . 319 323 + BccI CCATC 327 328 . . 331 334 + TaiI ACGT 334 330 . . 331 334 + SetI ASST 334 330 . . 331 334 + MaeII ACGT 331 333 . . 331 336 + AflIII ACRYGT 331 335 . . 335 340 + Hpy166II GTNNAC 337 337 . . 330 335 - BtrI CACGTC 332 332 . . 349 352 + TaiI ACGT 352 348 . . 349 352 + SetI ASST 352 348 . . 349 352 + MaeII ACGT 349 351 . . 351 355 + Tsp45I GTSAC 350 355 . . 351 355 + MaeIII GTNAC 350 355 . . 353 359 + AarI CACCTGC 363 367 . . 354 357 + SetI ASST 357 353 . . 354 359 + BspMI ACCTGC 363 367 . . 352 356 - HphI GGTGA 344 343 . . 369 374 + BetI* WCCGGW 369 373 . . 370 373 + HpaII CCGG 370 372 . . 372 377 + NlaIV GGNNCC 374 374 . . 372 376 + BmgT120I GGNCC 373 374 . . 372 376 + AvaII GGWCC 372 375 . . 372 376 + AsuI* GGNCC 372 375 . . 385 390 + NspBII* CMGCKG 387 387 . . 385 388 + AciI CCGC 385 387 . . 387 391 + BlsI GCNGC 389 388 . . 387 391 + BisI GCNGC 388 389 . . 387 391 + TseI GCWGC 387 390 . . 387 391 - BbvI GCAGC 374 378 . . 392 403 + TstI CACNNNNNNTCC 383 378 415 410 393 397 + Tsp4CI* ACNGT 395 394 . . 406 411 + NspBII* CMGCKG 408 408 . . 406 409 + AciI CCGC 406 408 . . 409 413 + DdeI CTNAG 409 412 . . 412 417 + TatI WGTACW 412 416 . . 412 417 + ScaI AGTACT 414 414 . . 413 416 + Csp6I GTAC 413 415 . . 409 413 - BspCNI CTCAG 401 399 . . 409 413 - BseMII CTCAG 400 398 . . 413 416 + RsaI GTAC 414 414 . . 419 422 + MnlI CCTC 429 428 . . 423 426 + SetI ASST 426 422 . . 421 425 - HphI GGTGA 413 412 . . 427 430 + DpnI GATC 428 428 . . 427 430 + BstKTI GATC 429 427 . . 427 430 + MboI GATC 426 430 . . 427 431 - BinI* GGATC 421 422 . . 433 438 + ClaI ATCGAT 434 436 . . 434 437 + TaqI TCGA 434 436 . . 436 440 + TfiI GAWTC 436 439 . . 436 440 + HinfI GANTC 436 439 . . 444 448 + BinI* GGATC 452 453 . . 445 455 + Hin4I GAYNNNNNVTC 436 431 468 463 445 448 + DpnI GATC 446 446 . . 445 448 + BstKTI GATC 447 445 . . 445 448 + MboI GATC 444 448 . . 454 458 + Hpy188I TCNGA 456 455 . . 455 460 + Eco57MI CTGRAG 476 474 . . 455 460 + Eco57I CTGAAG 476 474 . . 456 467 + BdaI TGANNNNNNTCA 445 443 479 477 460 463 + Csp6I GTAC 460 462 . . 460 463 + RsaI GTAC 461 461 . . 462 465 + SetI ASST 465 461 . . 463 466 + MnlI CCTC 473 472 . . 464 469 + SmlI CTYRAG 464 468 . . 456 467 - BdaI TGANNNNNNTCA 479 477 445 443 468 471 + SetI ASST 471 467 . . 468 471 + CviJI RGCY 469 469 . . 468 471 + AluI AGCT 469 469 . . 464 469 - Bce83I* CTTGAG 449 447 . . 470 474 + DdeI CTNAG 470 473 . . 479 482 + SetI ASST 482 478 . . 480 484 + HphI GGTGA 492 491 . . 511 514 + MseI TTAA 511 513 . . 517 521 + Hpy188I TCNGA 519 518 . . 520 524 + MboII GAAGA 532 531 . . 536 539 + FatI CATG 535 539 . . 536 539 + NlaIII CATG 539 535 . . 536 539 + CviAII CATG 536 538 . . 542 545 + MseI TTAA 542 544 . . 544 549 + ApoI RAATTY 544 548 . . 545 548 + TspEI AATT 544 548 . . 554 558 + Hpy188I TCNGA 556 555 . . 564 568 + MboII GAAGA 576 575 . . 580 584 + HphI GGTGA 592 591 . . 581 585 + Tsp45I GTSAC 580 585 . . 581 585 + MaeIII GTNAC 580 585 . . 597 602 + BclI TGATCA 597 601 . . 598 601 + DpnI GATC 599 599 . . 598 601 + BstKTI GATC 600 598 . . 598 601 + MboI GATC 597 601 . . 604 608 + HphI GGTGA 616 615 . . 629 633 + TspDTI ATGAA 644 642 . . 631 635 + MboII GAAGA 643 642 . . 636 641 + NspI RCATGY 640 636 . . 637 640 + FatI CATG 636 640 . . 637 640 + NlaIII CATG 640 636 . . 637 640 + CviAII CATG 637 639 . . 655 658 + SetI ASST 658 654 . . 656 660 + Hin4II* CCTTC 666 665 . . 677 681 - TspDTI ATGAA 667 665 . . 692 695 + FatI CATG 691 695 . . 692 695 + NlaIII CATG 695 691 . . 692 695 + CviAII CATG 692 694 . . 704 709 + ApoI RAATTY 704 708 . . 705 708 + TspEI AATT 704 708 . . 714 717 + TspEI AATT 713 717 . . 715 720 + VspI ATTAAT 716 718 . . 716 719 + MseI TTAA 716 718 . . 718 721 + TspEI AATT 717 721 . . 729 733 - FokI GGATG 715 719 . . 729 733 - BseGI GGATG 728 726 . . 736 740 + Tsp4CI* ACNGT 738 737 . . 764 767 + CviRI* TGCA 765 765 . . 777 782 + Hpy178III* TCNNGA 778 780 . . 782 785 + TspEI AATT 781 785 . . 784 787 + MseI TTAA 784 786 . . 798 801 + SetI ASST 801 797 . . 798 801 + CviJI RGCY 799 799 . . 798 801 + AluI AGCT 799 799 . . 809 812 + SetI ASST 812 808 . . 811 816 + SmlI CTYRAG 811 815 . . 811 816 + Bce83I* CTTGAG 832 830 . . 816 819 + Csp6I GTAC 816 818 . . 816 819 + RsaI GTAC 817 817 . . 819 823 + Hpy99I CGWCG 823 818 . . 821 825 + TspGWI ACGGA 836 834 . . 826 831 + TatI WGTACW 826 830 . . 827 830 + Csp6I GTAC 827 829 . . 827 830 + RsaI GTAC 828 828 . . 833 838 - EcoP15I CAGCAG 805 807 . . 849 852 + TspEI AATT 848 852 . . 853 857 + Tsp4CI* ACNGT 855 854 . . 864 867 + DpnI GATC 865 865 . . 864 867 + BstKTI GATC 866 864 . . 864 867 + MboI GATC 863 867 . . 875 878 + CviJI RGCY 876 876 . . 912 915 + CviJI RGCY 913 913 . . 912 915 + HaeIII GGCC 913 913 . . 917 922 + Hpy166II GTNNAC 919 919 . . 917 922 + HindII GTYRAC 919 919 . . 929 932 + MseI TTAA 929 931 . . 933 936 + SetI ASST 936 932 . . 940 945 + BetI* WCCGGW 940 944 . . 941 944 + HpaII CCGG 941 943 . . 943 947 + HphI GGTGA 955 954 . . 944 948 + Tsp45I GTSAC 943 948 . . 944 948 + MaeIII GTNAC 943 948 . . 956 959 + SetI ASST 959 955 . . 959 964 + SnaBI TACGTA 961 961 . . 959 964 + BsaAI YACGTR 961 961 . . 960 963 + TaiI ACGT 963 959 . . 960 963 + SetI ASST 963 959 . . 960 963 + MaeII ACGT 960 962 . . 974 977 + Csp6I GTAC 974 976 . . 974 977 + RsaI GTAC 975 975 . . 987 990 + FatI CATG 986 990 . . 987 990 + NlaIII CATG 990 986 . . 987 990 + CviAII CATG 987 989 . . 988 992 + TspDTI ATGAA 1003 1001 . . 1001 1004 + TspEI AATT 1000 1004 . . 1013 1018 + ApoI RAATTY 1013 1017 . . 1014 1017 + TspEI AATT 1013 1017 . . 1019 1022 + TspEI AATT 1018 1022 . . 1020 1025 + VspI ATTAAT 1021 1023 . . 1021 1024 + MseI TTAA 1021 1023 . . 1043 1048 + Hpy178III* TCNNGA 1044 1046 . . 1043 1048 + BspHI TCATGA 1043 1047 . . 1044 1047 + FatI CATG 1043 1047 . . 1044 1047 + NlaIII CATG 1047 1043 . . 1044 1047 + CviAII CATG 1044 1046 . . 1045 1049 + TspDTI ATGAA 1060 1058 . . 1047 1051 + TfiI GAWTC 1047 1050 . . 1047 1051 + HinfI GANTC 1047 1050 . . 1059 1063 + Tsp4CI* ACNGT 1061 1060 . . 1062 1066 - BslFI GGGAC 1047 1051 . . 1130 1133 + SetI ASST 1133 1129 . . 1132 1136 + MaeIII GTNAC 1131 1136 . . 1144 1147 + TspEI AATT 1143 1147 . . 1177 1187 + MwoI GCNNNNNNNGC 1183 1180 . . 1177 1181 + BlsI GCNGC 1179 1178 . . 1177 1181 + BisI GCNGC 1178 1179 . . 1177 1181 + BbvI GCAGC 1189 1193 . . 1177 1181 + TseI GCWGC 1177 1180 . . 1185 1188 + SetI ASST 1188 1184 . . 1185 1188 + CviJI RGCY 1186 1186 . . 1185 1188 + AluI AGCT 1186 1186 . . 1192 1197 + SspI AATATT 1194 1194 . . 1205 1208 + MaeI CTAG 1205 1207 . . 1210 1215 + ApoI RAATTY 1210 1214 . . 1211 1214 + TspEI AATT 1210 1214 . . 1215 1220 + SmlI CTYRAG 1215 1219 . . 1215 1220 - Bce83I* CTTGAG 1200 1198 . . 1221 1225 + HphI GGTGA 1233 1232 . . 1222 1227 + Hpy166II GTNNAC 1224 1224 . . 1230 1235 + BclI TGATCA 1230 1234 . . 1231 1234 + DpnI GATC 1232 1232 . . 1231 1234 + BstKTI GATC 1233 1231 . . 1231 1234 + MboI GATC 1230 1234 . . 1234 1243 + MslI CAYNNNNRTG 1238 1238 . . 1237 1242 + VspI ATTAAT 1238 1240 . . 1238 1241 + MseI TTAA 1238 1240 . . 1241 1245 + TspDTI ATGAA 1256 1254 . . 1243 1247 + TfiI GAWTC 1243 1246 . . 1243 1247 + HinfI GANTC 1243 1246 . . 1249 1253 + Tsp4CI* ACNGT 1251 1250 . . 1254 1258 - TspDTI ATGAA 1244 1242 . . 1266 1271 + SmlI CTYRAG 1266 1270 . . 1266 1271 + AflII CTTAAG 1266 1270 . . 1267 1270 + MseI TTAA 1267 1269 . . 1271 1276 + BtgZI GCGATG 1286 1290 . . 1285 1289 + DdeI CTNAG 1285 1288 . . 1291 1295 + DdeI CTNAG 1291 1294 . . 1295 1298 + CviJI RGCY 1296 1296 . . 1295 1298 + HaeIII GGCC 1296 1296 . . 1296 1301 + Cac8I GCNNGC 1298 1298 . . 1310 1314 + TfiI GAWTC 1310 1313 . . 1310 1314 + HinfI GANTC 1310 1313 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 363 954 # # AciI: # [CCGC] # 21 385 911 # # AflII: # [CTTAAG] # 51 1266 # # AflIII: # [ACRYGT] # 331 986 # # AluI: # [AGCT] # 75 102 109 131 183 330 # 387 # # ApoI: # [RAATTY] # 107 124 160 197 309 420 # # AsuI*: # [GGNCC] # 372 945 # # AvaII: # [GGWCC] # 372 945 # # BbvI: # [GCAGC] # 128 374 815 # # BccI: # [CCATC] # 121 206 990 # # Bce83I*: # [CTTGAG] # 117 368 383 449 # # BceAI: # [ACGGC] # 85 194 1038 # # BclI: # [TGATCA] # 87 597 633 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 445 838 # # BetI*: # [WCCGGW] # 120 249 377 571 # # BfiI: # [ACTGGG] # 309 1008 # # BinI*: # [GGATC] # 31 85 336 865 # # BisI: # [GCNGC] # 139 388 790 # # BlsI: # [GCNGC] # 138 389 790 # # BmgT120I: # [GGNCC] # 373 944 # # BsaAI: # [YACGTR] # 356 961 # # BseBI: # [CCWGG] # 173 1144 # # BseGI: # [GGATG] # 589 728 # # BseMII: # [CTCAG] # 400 917 # # BseRI: # [GAGGAG] # 203 1114 # # BseSI: # [GKGCMC] # 306 1011 # # BsiYI*: # [CCNNNNNNNGG] # 244 1073 # # BslFI: # [GGGAC] # 189 270 858 # # BsmAI: # [GTCTC] # 56 76 81 1104 # # BspCNI: # [CTCAG] # 401 916 # # BspHI: # [TCATGA] # 274 1043 # # BspMI: # [ACCTGC] # 135 228 954 # # BsrI: # [ACTGG] # 304 1013 # # BssKI: # [CCNGG] # 171 1146 # # BstKTI: # [GATC] # 18 84 93 153 266 336 # 367 # # BtgZI: # [GCGATG] # 31 1286 # # BtrI: # [CACGTC] # 332 985 # # Cac8I: # [GCNNGC] # 19 1298 # # ClaI: # [ATCGAT] # 434 883 # # Csp6I: # [GTAC] # 11 23 47 147 161 229 # 343 356 # # CviAII: # [CATG] # 40 55 57 62 101 165 # 269 273 295 # # CviJI: # [RGCY] # 6 9 21 37 75 77 # 100 102 110 177 273 330 # # CviRI*: # [TGCA] # 552 765 # # DdeI: # [CTNAG] # 6 26 61 68 75 266 # 815 # # DpnI: # [GATC] # 18 85 92 153 266 336 # 367 # # Eco57I: # [CTGAAG] # 209 267 841 # # Eco57MI: # [CTGRAG] # 209 267 841 # # EcoP15I: # [CAGCAG] # 512 805 # # EcoRII: # [CCWGG] # 171 1146 # # FatI: # [CATG] # 39 55 57 62 101 165 # 269 274 295 # # FokI: # [GGATG] # 602 715 # # GlaI: # [GCGC] # 48 1269 # # HaeII: # [RGCGCY] # 50 1267 # # HaeIII: # [GGCC] # 21 383 913 # # HhaI: # [GCGC] # 49 1268 # # Hin4I: # [GAYNNNNNVTC] # 32 436 849 # # Hin4II*: # [CCTTC] # 651 666 # # Hin6I: # [GCGC] # 47 1270 # # HindII: # [GTYRAC] # 398 919 # # HinfI: # [GANTC] # 4 7 67 132 137 163 # 196 611 # # HpaII: # [CCGG] # 121 249 376 571 # # HphI: # [GGTGA] # 24 69 79 84 100 278 # 339 344 # # Hpy166II: # [GTNNAC] # 93 305 337 582 # # Hpy178III*: # [TCNNGA] # 88 102 266 273 588 # # Hpy188I: # [TCNGA] # 37 63 456 761 # # Hpy99I: # [CGWCG] # 494 823 # # MaeI: # [CTAG] # 112 1205 # # MaeII: # [ACGT] # 18 331 357 611 # # MaeIII: # [GTNAC] # 60 186 188 230 290 363 # # MboI: # [GATC] # 18 87 90 153 266 336 # 367 # # MboII: # [GAAGA] # 44 67 532 674 # # MlyI: # [GAGTC] # 267 1050 # # MnlI: # [CCTC] # 21 44 184 224 844 # # MseI: # [TTAA] # 29 31 50 68 92 145 # 149 174 217 362 # # MslI: # [CAYNNNNRTG] # 79 203 1035 # # MwoI: # [GCNNNNNNNGC] # 106 134 1077 # # NlaIII: # [CATG] # 43 55 57 62 101 165 # 269 270 295 # # NlaIV: # [GGNNCC] # 374 943 # # NspBII*: # [CMGCKG] # 21 184 203 909 # # NspI: # [RCATGY] # 640 677 # # PflMI: # [CCANNNNNTGG] # 244 1073 # # PleI: # [GAGTC] # 267 1050 # # PvuII: # [CAGCTG] # 184 1133 # # RsaI: # [GTAC] # 11 23 47 147 161 230 # 342 356 # # ScaI: # [AGTACT] # 414 903 # # ScrFI: # [CCNGG] # 173 1144 # # SduI: # [GDGCHC] # 306 1011 # # SecI*: # [CCNNGG] # 172 1145 # # SetI: # [ASST] # 4 5 6 11 11 12 # 15 18 23 25 36 38 # 39 46 51 55 69 111 # 124 129 143 170 176 # # SmlI: # [CTYRAG] # 51 51 347 404 464 # # SnaBI: # [TACGTA] # 356 961 # # SspI: # [AATATT] # 123 1194 # # TaiI: # [ACGT] # 18 334 354 611 # # TaqI: # [TCGA] # 434 883 # # TatI: # [WGTACW] # 412 414 491 # # TfiI: # [GAWTC] # 4 7 67 132 196 300 # 611 # # TseI: # [GCWGC] # 140 387 790 # # Tsp45I: # [GTSAC] # 230 350 363 374 # # Tsp4CI*: # [ACNGT] # 66 117 144 190 206 251 # 343 # # TspDTI: # [ATGAA] # 12 23 57 61 184 336 # 644 # # TspEI: # [AATT] # 4 5 9 10 13 13 # 26 64 67 67 101 107 # 125 152 160 394 # # TspGWI: # [ACGGA] # 83 481 753 # # TstI: # [CACNNNNNNTCC] # 32 383 902 # # VspI: # [ATTAAT] # 79 217 305 716 # # XhoII: # [RGATCY] # 90 1227 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1317 # Reported_sequences: 1 # Reported_hitcount: 304 #---------------------------------------