Restriction Fragments for YBL055C



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The currently selected gene/sequence is :

YBL055C on chromosome II from coordinates 116829 to 115573.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.27440.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 12 + SfaNI GCATC 17 21 . . 23 26 + MnlI CCTC 33 32 . . 25 28 + TaqI TCGA 25 27 . . 41 44 + MaeI CTAG 41 43 . . 44 47 + CviJI RGCY 45 45 . . 47 52 + Hpy178III* TCNNGA 48 50 . . 57 60 + TspEI AATT 56 60 . . 68 73 + SspI AATATT 70 70 . . 78 81 + FatI CATG 77 81 . . 78 81 + NlaIII CATG 81 77 . . 78 81 + CviAII CATG 78 80 . . 92 97 + DsaI* CCRYGG 92 96 . . 92 97 + SecI* CCNNGG 92 96 . . 93 104 + TstI CACNNNNNNTCC 84 79 116 111 94 98 + TspGWI ACGGA 109 107 . . 94 104 + BsaXI ACNNNNNCTCC 84 81 114 111 97 101 + TfiI GAWTC 97 100 . . 97 101 + HinfI GANTC 97 100 . . 103 108 + NspBII* CMGCKG 105 105 . . 103 106 + AciI CCGC 103 105 . . 129 133 + TfiI GAWTC 129 132 . . 129 133 + HinfI GANTC 129 132 . . 132 136 + Hpy188I TCNGA 134 133 . . 138 143 + XhoII RGATCY 138 142 . . 139 142 + DpnI GATC 140 140 . . 139 142 + BstKTI GATC 141 139 . . 139 142 + MboI GATC 138 142 . . 139 143 - BinI* GGATC 133 134 . . 152 156 + Tsp4CI* ACNGT 154 153 . . 174 177 + Csp6I GTAC 174 176 . . 174 177 + RsaI GTAC 175 175 . . 177 181 + BccI CCATC 185 186 . . 178 182 - FokI GGATG 164 168 . . 182 187 + EcoP15I CAGCAG 212 214 . . 178 182 - BseGI GGATG 177 175 . . 197 200 + TspEI AATT 196 200 . . 207 212 + Cac8I GCNNGC 209 209 . . 211 215 + BlsI GCNGC 213 212 . . 211 215 + BisI GCNGC 212 213 . . 211 215 + TseI GCWGC 211 214 . . 215 219 + DdeI CTNAG 215 218 . . 215 219 + BspCNI CTCAG 228 226 . . 215 219 + BseMII CTCAG 229 227 . . 211 215 - BbvI GCAGC 198 202 . . 216 220 + Hpy188I TCNGA 218 217 . . 219 222 - MnlI CCTC 212 211 . . 223 228 + BsaAI YACGTR 225 225 . . 223 228 + PmaCI CACGTG 225 225 . . 224 227 + TaiI ACGT 227 223 . . 224 227 + SetI ASST 227 223 . . 224 227 + MaeII ACGT 224 226 . . 241 245 + MaeIII GTNAC 240 245 . . 247 251 + BinI* GGATC 255 256 . . 248 251 + DpnI GATC 249 249 . . 248 251 + BstKTI GATC 250 248 . . 248 251 + MboI GATC 247 251 . . 251 255 - FokI GGATG 237 241 . . 251 255 - BseGI GGATG 250 248 . . 262 266 + PleI GAGTC 270 271 . . 262 266 + MlyI GAGTC 271 271 . . 262 266 + HinfI GANTC 262 265 . . 259 262 - AciI CCGC 259 261 . . 264 268 + BsmAI GTCTC 269 273 . . 275 286 + BstXI CCANNNNNNTGG 282 278 . . 290 294 + BlsI GCNGC 292 291 . . 290 294 + BisI GCNGC 291 292 . . 290 294 + BbvI GCAGC 302 306 . . 290 294 + TseI GCWGC 290 293 . . 293 297 - HgaI GACGC 282 287 . . 300 305 + XhoII RGATCY 300 304 . . 300 305 + BglII AGATCT 300 304 . . 301 304 + DpnI GATC 302 302 . . 301 304 + BstKTI GATC 303 301 . . 301 304 + MboI GATC 300 304 . . 304 308 + DdeI CTNAG 304 307 . . 307 310 + CviJI RGCY 308 308 . . 313 316 + MseI TTAA 313 315 . . 317 320 + SetI ASST 320 316 . . 317 320 + CviJI RGCY 318 318 . . 317 320 + AluI AGCT 318 318 . . 333 336 + SetI ASST 336 332 . . 337 342 + Hpy166II GTNNAC 339 339 . . 340 345 + TaqII CACCCA 356 354 . . 339 343 - HphI GGTGA 331 330 . . 344 347 + FatI CATG 343 347 . . 344 347 + NlaIII CATG 347 343 . . 344 347 + CviAII CATG 344 346 . . 352 357 + Hpy166II GTNNAC 354 354 . . 352 357 + HpaI GTTAAC 354 354 . . 352 357 + HindII GTYRAC 354 354 . . 353 356 + MseI TTAA 353 355 . . 366 370 + FokI GGATG 379 383 . . 366 370 + BseGI GGATG 372 370 . . 364 367 - AciI CCGC 364 366 . . 367 371 - SfaNI GCATC 357 361 . . 371 374 + MaeI CTAG 371 373 . . 380 384 + BslFI GGGAC 394 398 . . 387 390 + CviJI RGCY 388 388 . . 388 393 + Cac8I GCNNGC 390 390 . . 388 393 + BmtI GCTAGC 392 388 . . 388 393 + NheI GCTAGC 388 392 . . 389 392 + MaeI CTAG 389 391 . . 391 396 + HaeII RGCGCY 395 391 . . 391 396 + Eco47III AGCGCT 393 393 . . 392 395 + Hin6I GCGC 392 394 . . 392 395 + GlaI GCGC 393 393 . . 392 395 + HhaI GCGC 394 392 . . 408 418 + BsiYI* CCNNNNNNNGG 414 411 . . 409 413 + Hin4II* CCTTC 419 418 . . 413 418 + DsaI* CCRYGG 413 417 . . 413 418 + StyI CCWWGG 413 417 . . 413 418 + NcoI CCATGG 413 417 . . 413 418 + SecI* CCNNGG 413 417 . . 414 417 + FatI CATG 413 417 . . 414 417 + NlaIII CATG 417 413 . . 414 417 + CviAII CATG 414 416 . . 417 421 + FokI GGATG 430 434 . . 417 421 + BseGI GGATG 423 421 . . 419 423 + TspDTI ATGAA 434 432 . . 433 437 + PleI GAGTC 441 442 . . 433 437 + MlyI GAGTC 442 442 . . 433 437 + HinfI GANTC 433 436 . . 435 439 + BsmAI GTCTC 440 444 . . 463 467 + BccI CCATC 471 472 . . 471 474 + CviRI* TGCA 472 472 . . 479 484 + Hpy166II GTNNAC 481 481 . . 491 494 + SetI ASST 494 490 . . 491 494 + CviJI RGCY 492 492 . . 491 494 + AluI AGCT 492 492 . . 505 509 + TspDTI ATGAA 520 518 . . 507 511 + TfiI GAWTC 507 510 . . 507 511 + HinfI GANTC 507 510 . . 513 518 + Cac8I GCNNGC 515 515 . . 522 525 + SetI ASST 525 521 . . 523 526 + MnlI CCTC 533 532 . . 525 530 + Hpy178III* TCNNGA 526 528 . . 525 530 + BspHI TCATGA 525 529 . . 526 529 + FatI CATG 525 529 . . 526 529 + NlaIII CATG 529 525 . . 526 529 + CviAII CATG 526 528 . . 541 544 + SetI ASST 544 540 . . 545 550 + MfeI CAATTG 545 549 . . 546 549 + TspEI AATT 545 549 . . 550 554 + HphI GGTGA 562 561 . . 555 558 + DpnI GATC 556 556 . . 555 558 + BstKTI GATC 557 555 . . 555 558 + MboI GATC 554 558 . . 561 566 - MmeI TCCRAC 542 540 . . 582 587 + SfeI* CTRYAG 582 586 . . 599 602 + CviRI* TGCA 600 600 . . 597 601 - SfaNI GCATC 587 591 . . 605 608 + SetI ASST 608 604 . . 616 620 + MboII GAAGA 628 627 . . 616 621 - Ksp632I* CTCTTC 611 614 . . 616 622 - SapI GCTCTTC 611 614 . . 652 656 + DdeI CTNAG 652 655 . . 652 656 + BspCNI CTCAG 665 663 . . 652 656 + BseMII CTCAG 666 664 . . 654 659 + EcoP15I CAGCAG 684 686 . . 656 660 + BlsI GCNGC 658 657 . . 656 660 + BisI GCNGC 657 658 . . 656 660 + BbvI GCAGC 668 672 . . 656 660 + TseI GCWGC 656 659 . . 658 661 + SetI ASST 661 657 . . 658 661 + CviJI RGCY 659 659 . . 658 661 + AluI AGCT 659 659 . . 674 677 + CviRI* TGCA 675 675 . . 676 681 + NdeI CATATG 677 679 . . 686 689 + Hin6I GCGC 686 688 . . 686 689 + GlaI GCGC 687 687 . . 686 689 + HhaI GCGC 688 686 . . 688 693 + NspI RCATGY 692 688 . . 689 692 + FatI CATG 688 692 . . 689 692 + NlaIII CATG 692 688 . . 689 692 + CviAII CATG 689 691 . . 692 696 + Tsp45I GTSAC 691 696 . . 692 696 + MaeIII GTNAC 691 696 . . 708 713 + SspI AATATT 710 710 . . 728 732 + BssKI CCNGG 727 732 . . 728 731 + HpaII CCGG 728 730 . . 728 732 + ScrFI CCNGG 729 730 . . 728 732 + CauII* CCSGG 729 730 . . 732 737 + Hpy166II GTNNAC 734 734 . . 735 741 + TspRI CASTGNN 741 732 . . 744 747 - MnlI CCTC 737 736 . . 751 754 + SetI ASST 754 750 . . 747 752 - BciVI GTATCC 741 740 . . 758 761 + SetI ASST 761 757 . . 758 761 + CviJI RGCY 759 759 . . 758 761 + AluI AGCT 759 759 . . 771 774 + SetI ASST 774 770 . . 774 777 + CviRI* TGCA 775 775 . . 775 779 + SfaNI GCATC 784 788 . . 779 783 + Hpy99I CGWCG 783 778 . . 785 788 + MaeI CTAG 785 787 . . 788 791 - AciI CCGC 788 790 . . 788 792 - FauI CCCGC 781 783 . . 799 804 + ApoI RAATTY 799 803 . . 800 803 + TspEI AATT 799 803 . . 803 807 - TspDTI ATGAA 793 791 . . 807 812 + Hpy178III* TCNNGA 808 810 . . 805 809 - FokI GGATG 791 795 . . 805 809 - BseGI GGATG 804 802 . . 819 824 + SpeI ACTAGT 819 823 . . 820 823 + MaeI CTAG 820 822 . . 825 829 + BmgT120I GGNCC 826 827 . . 825 829 + AvaII GGWCC 825 828 . . 825 829 + AsuI* GGNCC 825 828 . . 828 842 + XcmI CCANNNNNNNNNTGG 835 834 . . 831 835 - TspDTI ATGAA 821 819 . . 837 843 + TspRI CASTGNN 843 834 . . 838 842 + BsrI ACTGG 843 841 . . 848 859 + BcgI CGANNNNNNTGC 837 835 871 869 857 860 + CviRI* TGCA 858 858 . . 863 866 + TspEI AATT 862 866 . . 878 883 + TaqII CACCCA 894 892 . . 877 881 - HphI GGTGA 869 868 . . 887 891 - MboII GAAGA 879 878 . . 889 893 - TspDTI ATGAA 879 877 . . 898 903 + Hpy166II GTNNAC 900 900 . . 902 906 + Tsp4CI* ACNGT 904 903 . . 920 925 + SecI* CCNNGG 920 924 . . 922 925 - MnlI CCTC 915 914 . . 931 934 + MaeI CTAG 931 933 . . 933 936 + SetI ASST 936 932 . . 933 936 + CviJI RGCY 934 934 . . 933 936 + AluI AGCT 934 934 . . 961 964 + SetI ASST 964 960 . . 975 985 + Hin4I GAYNNNNNVTC 966 961 998 993 976 986 + BsaXI ACNNNNNCTCC 966 963 996 993 973 977 - BsmAI GTCTC 967 971 . . 979 983 - SfaNI GCATC 969 973 . . 985 990 + DsaI* CCRYGG 985 989 . . 985 990 + SecI* CCNNGG 985 989 . . 984 988 - TspGWI ACGGA 974 972 . . 998 1001 + MseI TTAA 998 1000 . . 1008 1013 + NspI RCATGY 1012 1008 . . 1008 1013 + Cac8I GCNNGC 1010 1010 . . 1008 1013 + SphI GCATGC 1012 1008 . . 1009 1012 + FatI CATG 1008 1012 . . 1009 1012 + NlaIII CATG 1012 1008 . . 1009 1012 + CviAII CATG 1009 1011 . . 1010 1015 + EcoT22I ATGCAT 1014 1010 . . 1011 1014 + CviRI* TGCA 1012 1012 . . 1012 1016 + SfaNI GCATC 1021 1025 . . 1022 1027 + TatI WGTACW 1022 1026 . . 1022 1027 + ScaI AGTACT 1024 1024 . . 1023 1026 + Csp6I GTAC 1023 1025 . . 1023 1026 + RsaI GTAC 1024 1024 . . 1020 1024 - BsrI ACTGG 1020 1018 . . 1026 1030 + DdeI CTNAG 1026 1029 . . 1028 1033 + TsoI TARCCA 1044 1042 . . 1029 1032 + CviJI RGCY 1030 1030 . . 1038 1042 + BssKI CCNGG 1037 1042 . . 1038 1042 + BseBI CCWGG 1039 1040 . . 1038 1042 + ScrFI CCNGG 1039 1040 . . 1038 1042 + EcoRII CCWGG 1037 1042 . . 1043 1046 + SetI ASST 1046 1042 . . 1042 1045 - MnlI CCTC 1035 1034 . . 1054 1059 + AsuII TTCGAA 1055 1057 . . 1055 1058 + TaqI TCGA 1055 1057 . . 1056 1067 + BcgI CGANNNNNNTGC 1045 1043 1079 1077 1065 1068 + CviRI* TGCA 1066 1066 . . 1066 1071 - BsmI GAATGC 1066 1064 . . 1075 1079 - TspGWI ACGGA 1065 1063 . . 1079 1082 + MseI TTAA 1079 1081 . . 1091 1094 + SetI ASST 1094 1090 . . 1091 1094 + CviJI RGCY 1092 1092 . . 1091 1094 + AluI AGCT 1092 1092 . . 1092 1097 + Cac8I GCNNGC 1094 1094 . . 1107 1112 + BsmI GAATGC 1113 1111 . . 1110 1113 + CviRI* TGCA 1111 1111 . . 1114 1118 + MboII GAAGA 1126 1125 . . 1125 1128 + FatI CATG 1124 1128 . . 1125 1128 + NlaIII CATG 1128 1124 . . 1125 1128 + CviAII CATG 1125 1127 . . 1134 1138 + BmgT120I GGNCC 1135 1136 . . 1134 1138 + AsuI* GGNCC 1134 1137 . . 1135 1138 + CviJI RGCY 1136 1136 . . 1135 1138 + HaeIII GGCC 1136 1136 . . 1136 1140 - BceAI ACGGC 1121 1123 . . 1145 1148 + CviJI RGCY 1146 1146 . . 1169 1174 + MfeI CAATTG 1169 1173 . . 1170 1173 + TspEI AATT 1169 1173 . . 1180 1184 + Hpy188I TCNGA 1182 1181 . . 1193 1196 + TaiI ACGT 1196 1192 . . 1193 1196 + SetI ASST 1196 1192 . . 1193 1196 + MaeII ACGT 1193 1195 . . 1197 1202 + XhoII RGATCY 1197 1201 . . 1192 1197 - BtrI CACGTC 1194 1194 . . 1197 1201 + BinI* GGATC 1205 1206 . . 1198 1201 + DpnI GATC 1199 1199 . . 1198 1201 + BstKTI GATC 1200 1198 . . 1198 1201 + MboI GATC 1197 1201 . . 1203 1206 + CviJI RGCY 1204 1204 . . 1202 1207 - TsoI TARCCA 1192 1190 . . 1221 1226 + BsaAI YACGTR 1223 1223 . . 1221 1226 + PmaCI CACGTG 1223 1223 . . 1222 1225 + TaiI ACGT 1225 1221 . . 1222 1225 + SetI ASST 1225 1221 . . 1222 1225 + MaeII ACGT 1222 1224 . . 1227 1231 + MboII GAAGA 1239 1238 . . 1227 1232 + BbvII* GAAGAC 1234 1238 . . 1234 1237 + SetI ASST 1237 1233 . . #--------------------------------------- # # # AciI: # [CCGC] # 103 105 156 424 469 # # AluI: # [AGCT] # 100 158 165 167 174 175 # 318 # # ApoI: # [RAATTY] # 458 799 # # AsuI*: # [GGNCC] # 123 309 825 # # AsuII: # [TTCGAA] # 202 1055 # # AvaII: # [GGWCC] # 432 825 # # BbvI: # [GCAGC] # 104 198 366 589 # # BbvII*: # [GAAGAC] # 23 1234 # # BccI: # [CCATC] # 185 286 786 # # BceAI: # [ACGGC] # 136 1121 # # BcgI: # [CGANNNNNNTGC] # 34 34 174 178 837 # # BciVI: # [GTATCC] # 516 741 # # BglII: # [AGATCT] # 300 957 # # BinI*: # [GGATC] # 52 122 133 950 # # BisI: # [GCNGC] # 79 212 366 600 # # BlsI: # [GCNGC] # 79 213 366 599 # # BmgT120I: # [GGNCC] # 122 309 826 # # BmtI: # [GCTAGC] # 392 865 # # BsaAI: # [YACGTR] # 34 225 998 # # BsaXI: # [ACNNNNNCTCC] # 30 30 84 261 852 # # BseBI: # [CCWGG] # 218 1039 # # BseGI: # [GGATG] # 51 73 122 177 381 453 # # BseMII: # [CTCAG] # 229 437 591 # # BsiYI*: # [CCNNNNNNNGG] # 414 843 # # BslFI: # [GGGAC] # 394 863 # # BsmAI: # [GTCTC] # 171 269 290 527 # # BsmI: # [GAATGC] # 47 144 1066 # # BspCNI: # [CTCAG] # 228 437 592 # # BspHI: # [TCATGA] # 525 732 # # BsrI: # [ACTGG] # 177 237 843 # # BssKI: # [CCNGG] # 220 310 727 # # BstKTI: # [GATC] # 53 57 109 141 254 643 # # BstXI: # [CCANNNNNNTGG] # 282 975 # # BtrI: # [CACGTC] # 63 1194 # # Cac8I: # [GCNNGC] # 84 125 163 181 209 495 # # CauII*: # [CCSGG] # 528 729 # # Csp6I: # [GTAC] # 174 234 849 # # CviAII: # [CATG] # 70 78 112 116 132 163 # 266 320 # # CviJI: # [RGCY] # 10 10 44 45 53 58 # 62 70 96 100 104 167 # 175 263 # # CviRI*: # [TGCA] # 45 54 75 83 100 128 # 146 154 472 # # DdeI: # [CTNAG] # 89 215 231 348 374 # # DpnI: # [GATC] # 53 58 109 140 254 643 # # DsaI*: # [CCRYGG] # 92 272 321 572 # # Eco47III: # [AGCGCT] # 393 864 # # EcoP15I: # [CAGCAG] # 212 472 573 # # EcoRII: # [CCWGG] # 220 1037 # # EcoT22I: # [ATGCAT] # 243 1014 # # FatI: # [CATG] # 70 77 112 116 133 163 # 266 320 # # FauI: # [CCCGC] # 476 781 # # FokI: # [GGATG] # 51 73 142 164 361 466 # # GlaI: # [GCGC] # 294 393 570 # # HaeII: # [RGCGCY] # 395 862 # # HaeIII: # [GGCC] # 121 1136 # # HgaI: # [GACGC] # 282 975 # # HhaI: # [GCGC] # 294 394 569 # # Hin4I: # [GAYNNNNNVTC] # 32 259 966 # # Hin4II*: # [CCTTC] # 419 838 # # Hin6I: # [GCGC] # 294 392 571 # # HindII: # [GTYRAC] # 354 903 # # HinfI: # [GANTC] # 32 74 97 133 171 750 # # HpaI: # [GTTAAC] # 354 903 # # HpaII: # [CCGG] # 529 728 # # HphI: # [GGTGA] # 231 307 331 388 # # Hpy166II: # [GTNNAC] # 15 127 166 253 339 357 # # Hpy178III*: # [TCNNGA] # 48 282 449 478 # # Hpy188I: # [TCNGA] # 75 84 134 964 # # Hpy99I: # [CGWCG] # 474 783 # # Ksp632I*: # [CTCTTC] # 611 646 # # MaeI: # [CTAG] # 18 35 41 111 326 330 # 396 # # MaeII: # [ACGT] # 29 35 224 969 # # MaeIII: # [GTNAC] # 240 451 566 # # MboI: # [GATC] # 53 60 109 138 254 643 # # MboII: # [GAAGA] # 18 113 247 251 628 # # MfeI: # [CAATTG] # 88 545 624 # # MlyI: # [GAGTC] # 171 271 815 # # MmeI: # [TCCRAC] # 542 715 # # MnlI: # [CCTC] # 33 120 178 179 204 222 # 321 # # MseI: # [TTAA] # 40 81 178 313 645 # # NcoI: # [CCATGG] # 413 844 # # NdeI: # [CATATG] # 580 677 # # NheI: # [GCTAGC] # 388 869 # # NlaIII: # [CATG] # 70 81 112 116 129 163 # 266 320 # # NspBII*: # [CMGCKG] # 105 1152 # # NspI: # [RCATGY] # 245 320 692 # # PleI: # [GAGTC] # 171 270 816 # # PmaCI: # [CACGTG] # 34 225 998 # # RsaI: # [GTAC] # 175 233 849 # # SapI: # [GCTCTTC] # 611 646 # # ScaI: # [AGTACT] # 233 1024 # # ScrFI: # [CCNGG] # 218 310 729 # # SecI*: # [CCNNGG] # 65 92 272 321 507 # # SetI: # [ASST] # 7 12 13 16 19 20 # 28 29 31 48 53 64 # 82 93 93 102 158 162 # 227 # # SfaNI: # [GCATC] # 17 52 185 197 230 236 # 340 # # SfeI*: # [CTRYAG] # 582 675 # # SpeI: # [ACTAGT] # 438 819 # # SphI: # [GCATGC] # 245 1012 # # SspI: # [AATATT] # 70 547 640 # # StyI: # [CCWWGG] # 413 844 # # TaiI: # [ACGT] # 29 32 227 969 # # TaqI: # [TCGA] # 25 202 1030 # # TaqII: # [CACCCA] # 356 363 538 # # TatI: # [WGTACW] # 235 1022 # # TfiI: # [GAWTC] # 32 97 378 750 # # TseI: # [GCWGC] # 79 211 366 601 # # TsoI: # [TARCCA] # 65 148 1044 # # Tsp45I: # [GTSAC] # 566 691 # # Tsp4CI*: # [ACNGT] # 154 353 750 # # TspDTI: # [ATGAA] # 28 58 86 273 378 434 # # TspEI: # [AATT] # 56 63 88 140 254 307 # 349 # # TspGWI: # [ACGGA] # 91 109 192 865 # # TspRI: # [CASTGNN] # 102 414 741 # # TstI: # [CACNNNNNNTCC] # 32 84 1141 # # XcmI: # [CCANNNNNNNNNTGG] # 422 835 # # XhoII: # [RGATCY] # 60 138 162 897 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1257 # Reported_sequences: 1 # Reported_hitcount: 304 #---------------------------------------