Restriction Fragments for YARCdelta4



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YARCdelta4 on chromosome I from coordinates 160574 to 160238.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.24046.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 80 84 + HgaI GACGC 89 94 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 108 111 + TspEI AATT 107 111 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 148 152 + BinI* GGATC 156 157 . . 149 152 + DpnI GATC 150 150 . . 149 152 + BstKTI GATC 151 149 . . 149 152 + MboI GATC 148 152 . . 154 158 + TspDTI ATGAA 169 167 . . 173 177 + TspGWI ACGGA 188 186 . . 180 183 - MnlI CCTC 173 172 . . 180 190 - Hin4I GAYNNNNNVTC 203 198 171 166 193 198 + SspI AATATT 195 195 . . 215 219 + TfiI GAWTC 215 218 . . 215 219 + HinfI GANTC 215 218 . . 219 229 + BsiYI* CCNNNNNNNGG 225 222 . . 229 233 + TfiI GAWTC 229 232 . . 226 229 - MnlI CCTC 219 218 . . 229 233 + HinfI GANTC 229 232 . . 240 247 + PspXI VCTCGAGB 241 245 . . 240 243 + MnlI CCTC 250 249 . . 240 247 + AbsI CCTCGAGG 241 245 . . 241 246 + SmlI CTYRAG 241 245 . . 241 246 + XhoI CTCGAG 241 245 . . 241 246 + BmeT110I CYCGRG 242 244 . . 241 246 + AvaI CYCGRG 241 245 . . 242 245 + TaqI TCGA 242 244 . . 244 249 + BseRI GAGGAG 259 257 . . 244 247 - MnlI CCTC 237 236 . . 255 258 + MaeI CTAG 255 257 . . 267 272 + Hpy166II GTNNAC 269 269 . . 267 272 + BssNAI GTATAC 269 269 . . 267 272 + AccI GTMKAC 268 270 . . 271 274 + SetI ASST 274 270 . . 275 280 + SspI AATATT 277 277 . . 283 286 + CviJI RGCY 284 284 . . 300 304 + TfiI GAWTC 300 303 . . 300 304 + HinfI GANTC 300 303 . . 310 313 + TspEI AATT 309 313 . . 324 328 - HphI GGTGA 316 315 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 241 # # AccI: # [GTMKAC] # 69 268 # # AluI: # [AGCT] # 119 218 # # AvaI: # [CYCGRG] # 96 241 # # BinI*: # [GGATC] # 156 181 # # BmeT110I: # [CYCGRG] # 95 242 # # BseRI: # [GAGGAG] # 78 259 # # BsiYI*: # [CCNNNNNNNGG] # 112 225 # # BssNAI: # [GTATAC] # 68 269 # # BstKTI: # [GATC] # 151 186 # # CviJI: # [RGCY] # 53 119 165 # # DpnI: # [GATC] # 150 187 # # HgaI: # [GACGC] # 89 248 # # Hin4I: # [GAYNNNNNVTC] # 32 32 56 83 134 # # HinfI: # [GANTC] # 14 37 71 215 # # HphI: # [GGTGA] # 21 316 # # Hpy166II: # [GTNNAC] # 68 269 # # MaeI: # [CTAG] # 15 33 82 207 # # MboI: # [GATC] # 148 189 # # MboII: # [GAAGA] # 86 251 # # MnlI: # [CCTC] # 13 18 46 87 173 # # PspXI: # [VCTCGAGB] # 96 241 # # SetI: # [ASST] # 63 121 153 # # SmlI: # [CTYRAG] # 96 241 # # SpeI: # [ACTAGT] # 15 32 290 # # SspI: # [AATATT] # 60 82 195 # # TaqI: # [TCGA] # 95 242 # # TfiI: # [GAWTC] # 14 37 71 215 # # Tsp4CI*: # [ACNGT] # 67 270 # # TspDTI: # [ATGAA] # 168 169 # # TspEI: # [AATT] # 28 107 202 # # TspGWI: # [ACGGA] # 149 188 # # XhoI: # [CTCGAG] # 96 241 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 337 # Reported_sequences: 1 # Reported_hitcount: 48 #---------------------------------------