Restriction Fragments for SEN34/YAR008W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SEN34/YAR008W on chromosome I from coordinates 158966 to 159793.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5471.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 + AciI CCGC 7 9 . . 10 13 + MaeI CTAG 10 12 . . 18 29 + BdaI TGANNNNNNTCA 7 5 41 39 25 28 + DpnI GATC 26 26 . . 25 28 + BstKTI GATC 27 25 . . 25 28 + MboI GATC 24 28 . . 18 29 - BdaI TGANNNNNNTCA 41 39 7 5 40 44 + DdeI CTNAG 40 43 . . 70 75 + Hpy178III* TCNNGA 71 73 . . 74 86 + PsrI GAACNNNNNNTAC 66 61 98 93 86 91 + PstI CTGCAG 90 86 . . 86 91 + SfeI* CTRYAG 86 90 . . 87 90 + CviRI* TGCA 88 88 . . 88 92 + BlsI GCNGC 90 89 . . 88 92 + BisI GCNGC 89 90 . . 88 92 + BbvI GCAGC 100 104 . . 88 92 + TseI GCWGC 88 91 . . 114 119 + KpnI GGTACC 118 114 . . 114 119 + NlaIV GGNNCC 116 116 . . 114 119 + HgiCI* GGYRCC 114 118 . . 114 119 + Acc65I GGTACC 114 118 . . 115 118 + Csp6I GTAC 115 117 . . 115 118 + RsaI GTAC 116 116 . . 117 120 + SetI ASST 120 116 . . 125 128 + CviJI RGCY 126 126 . . 123 126 - MnlI CCTC 116 115 . . 136 140 + MboII GAAGA 148 147 . . 144 154 + MwoI GCNNNNNNNGC 150 147 . . 149 152 + CviJI RGCY 150 150 . . 150 154 + BlsI GCNGC 152 151 . . 150 154 + BisI GCNGC 151 152 . . 150 154 + TseI GCWGC 150 153 . . 152 155 + CviRI* TGCA 153 153 . . 150 154 - BbvI GCAGC 137 141 . . 179 182 + TspEI AATT 178 182 . . 180 185 + VspI ATTAAT 181 183 . . 181 184 + MseI TTAA 181 183 . . 193 196 - MnlI CCTC 186 185 . . 211 214 - MnlI CCTC 204 203 . . 228 231 - AciI CCGC 228 230 . . 226 231 - BsrBI CCGCTC 228 228 . . 232 237 + NarI GGCGCC 233 235 . . 232 237 + KasI GGCGCC 232 236 . . 228 232 - FauI CCCGC 221 223 . . 232 237 + DinI GGCGCC 234 234 . . 232 237 + HaeII RGCGCY 236 232 . . 232 237 + NlaIV GGNNCC 234 234 . . 232 237 + AcyI GRCGYC 233 235 . . 232 237 + HgiCI* GGYRCC 232 236 . . 233 236 + Hin6I GCGC 233 235 . . 233 236 + GlaI GCGC 234 234 . . 233 236 + HhaI GCGC 235 233 . . 249 252 + TspEI AATT 248 252 . . 253 258 + Hpy166II GTNNAC 255 255 . . 253 258 + HindII GTYRAC 255 255 . . 258 263 + McrI* CGRYCG 261 259 . . 258 263 + PvuI CGATCG 261 259 . . 259 262 + DpnI GATC 260 260 . . 259 262 + BstKTI GATC 261 259 . . 259 262 + MboI GATC 258 262 . . 285 289 + Hpy188I TCNGA 287 286 . . 303 307 + FokI GGATG 316 320 . . 303 307 + BseGI GGATG 309 307 . . 305 309 + TspDTI ATGAA 320 318 . . 315 318 + CviRI* TGCA 316 316 . . 312 317 - BsrDI GCAATG 311 309 . . 320 323 + TspEI AATT 319 323 . . 322 325 + MseI TTAA 322 324 . . 338 342 + TfiI GAWTC 338 341 . . 338 342 + HinfI GANTC 338 341 . . 356 361 + PvuII CAGCTG 358 358 . . 356 361 + NspBII* CMGCKG 358 358 . . 357 360 + SetI ASST 360 356 . . 357 360 + CviJI RGCY 358 358 . . 357 360 + AluI AGCT 358 358 . . 359 364 + Eco57MI CTGRAG 380 378 . . 359 364 + Eco57I CTGAAG 380 378 . . 361 365 + MboII GAAGA 373 372 . . 365 368 + TspEI AATT 364 368 . . 368 371 + CviRI* TGCA 369 369 . . 372 376 + BceAI ACGGC 388 390 . . 374 377 + CviJI RGCY 375 375 . . 386 389 + MaeI CTAG 386 388 . . 401 404 + SetI ASST 404 400 . . 401 404 + CviJI RGCY 402 402 . . 401 404 + AluI AGCT 402 402 . . 405 408 + TspEI AATT 404 408 . . 409 413 + TfiI GAWTC 409 412 . . 409 413 + HinfI GANTC 409 412 . . 412 416 - MboII GAAGA 404 403 . . 421 425 - TspDTI ATGAA 411 409 . . 429 434 - BsrDI GCAATG 428 426 . . 439 442 + SetI ASST 442 438 . . 440 443 + MnlI CCTC 450 449 . . 451 454 + MseI TTAA 451 453 . . 455 459 - BsmAI GTCTC 449 453 . . 462 466 + TspGWI ACGGA 477 475 . . 469 473 + TfiI GAWTC 469 472 . . 469 473 + HinfI GANTC 469 472 . . 472 476 + Hpy188I TCNGA 474 473 . . 475 485 + Hin4I GAYNNNNNVTC 466 461 498 493 478 481 + SetI ASST 481 477 . . 478 481 + CviJI RGCY 479 479 . . 478 481 + AluI AGCT 479 479 . . 479 489 + MwoI GCNNNNNNNGC 485 482 . . 475 485 - Hin4I GAYNNNNNVTC 498 493 466 461 485 489 + FauI CCCGC 493 495 . . 486 489 + AciI CCGC 486 488 . . 487 490 + FnuDII* CGCG 488 488 . . 488 493 + BtgZI GCGATG 503 507 . . 493 498 + EcoRV GATATC 495 495 . . 498 504 + TspRI CASTGNN 504 495 . . 512 515 + MseI TTAA 512 514 . . 518 523 + TatI WGTACW 518 522 . . 519 522 + Csp6I GTAC 519 521 . . 519 522 + RsaI GTAC 520 520 . . 539 542 + CviRI* TGCA 540 540 . . 544 547 + SetI ASST 547 543 . . 571 574 + DpnI GATC 572 572 . . 571 574 + BstKTI GATC 573 571 . . 571 574 + MboI GATC 570 574 . . 579 582 + Csp6I GTAC 579 581 . . 579 582 + RsaI GTAC 580 580 . . 581 584 + TaiI ACGT 584 580 . . 581 584 + SetI ASST 584 580 . . 581 584 + MaeII ACGT 581 583 . . 591 596 + SecI* CCNNGG 591 595 . . 592 596 + BssKI CCNGG 591 596 . . 588 592 - BslFI GGGAC 573 577 . . 592 596 + BseBI CCWGG 593 594 . . 592 596 + ScrFI CCNGG 593 594 . . 592 596 + EcoRII CCWGG 591 596 . . 594 597 + SetI ASST 597 593 . . 596 601 + Hpy166II GTNNAC 598 598 . . 600 603 + TaiI ACGT 603 599 . . 600 603 + SetI ASST 603 599 . . 600 603 + MaeII ACGT 600 602 . . 627 632 + SecI* CCNNGG 627 631 . . 628 632 + BssKI CCNGG 627 632 . . 628 632 + BseBI CCWGG 629 630 . . 628 632 + ScrFI CCNGG 629 630 . . 628 632 + EcoRII CCWGG 627 632 . . 631 635 + HphI GGTGA 643 642 . . 634 637 + DpnI GATC 635 635 . . 634 637 + BstKTI GATC 636 634 . . 634 637 + MboI GATC 633 637 . . 637 640 + MnlI CCTC 647 646 . . 638 643 + Ksp632I* CTCTTC 644 647 . . 634 638 - BinI* GGATC 628 629 . . 639 643 - MboII GAAGA 631 630 . . 657 661 + Hpy188I TCNGA 659 658 . . 670 674 - SfaNI GCATC 660 664 . . 675 685 + Hin4I GAYNNNNNVTC 666 661 698 693 675 685 - Hin4I GAYNNNNNVTC 698 693 666 661 692 695 + CviJI RGCY 693 693 . . 700 710 + Hin4I GAYNNNNNVTC 691 686 723 718 701 704 + SetI ASST 704 700 . . 700 710 - Hin4I GAYNNNNNVTC 723 718 691 686 711 714 + FatI CATG 710 714 . . 711 714 + NlaIII CATG 714 710 . . 711 714 + CviAII CATG 711 713 . . 723 726 + CviRI* TGCA 724 724 . . 730 733 + MaeI CTAG 730 732 . . 739 743 + Tsp4CI* ACNGT 741 740 . . 733 744 - TstI CACNNNNNNTCC 757 752 725 720 764 767 - AciI CCGC 764 766 . . 775 779 + MboII GAAGA 787 786 . . 772 775 - AciI CCGC 772 774 . . 775 780 - Ksp632I* CTCTTC 770 773 . . 778 782 - BsmAI GTCTC 772 776 . . 797 801 - MboII GAAGA 789 788 . . 813 816 + CviJI RGCY 814 814 . . 825 828 + MseI TTAA 825 827 . . 822 827 - TsoI TARCCA 812 810 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 114 714 # # AciI: # [CCGC] # 7 8 56 221 258 278 # # AcyI: # [GRCGYC] # 233 595 # # AluI: # [AGCT] # 44 77 349 358 # # BbvI: # [GCAGC] # 37 100 691 # # BceAI: # [ACGGC] # 388 440 # # BdaI: # [TGANNNNNNTCA] # 0 0 7 34 787 # # BinI*: # [GGATC] # 200 628 # # BisI: # [GCNGC] # 62 89 677 # # BlsI: # [GCNGC] # 62 90 676 # # BseBI: # [CCWGG] # 36 199 593 # # BseGI: # [GGATG] # 309 519 # # BslFI: # [GGGAC] # 255 573 # # BsmAI: # [GTCTC] # 56 323 449 # # BsrBI: # [CCGCTC] # 228 600 # # BsrDI: # [GCAATG] # 117 311 400 # # BssKI: # [CCNGG] # 36 201 591 # # BstKTI: # [GATC] # 27 63 192 234 312 # # BtgZI: # [GCGATG] # 325 503 # # Csp6I: # [GTAC] # 60 115 249 404 # # CviAII: # [CATG] # 117 711 # # CviJI: # [RGCY] # 14 17 24 27 77 121 # 126 208 214 # # CviRI*: # [TGCA] # 53 65 88 104 163 171 # 184 # # DdeI: # [CTNAG] # 40 788 # # DinI: # [GGCGCC] # 234 594 # # DpnI: # [GATC] # 26 63 193 234 312 # # Eco57I: # [CTGAAG] # 380 448 # # Eco57MI: # [CTGRAG] # 380 448 # # EcoRII: # [CCWGG] # 36 201 591 # # EcoRV: # [GATATC] # 333 495 # # FatI: # [CATG] # 118 710 # # FauI: # [CCCGC] # 221 272 335 # # FnuDII*: # [CGCG] # 340 488 # # FokI: # [GGATG] # 316 512 # # GlaI: # [GCGC] # 234 594 # # HaeII: # [RGCGCY] # 236 592 # # HgiCI*: # [GGYRCC] # 114 118 596 # # HhaI: # [GCGC] # 235 593 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 7 25 25 32 105 168 # 466 # # Hin6I: # [GCGC] # 233 595 # # HindII: # [GTYRAC] # 255 573 # # HinfI: # [GANTC] # 60 71 338 359 # # HphI: # [GGTGA] # 185 643 # # Hpy166II: # [GTNNAC] # 230 255 343 # # Hpy178III*: # [TCNNGA] # 71 757 # # Hpy188I: # [TCNGA] # 169 185 187 287 # # KasI: # [GGCGCC] # 232 596 # # KpnI: # [GGTACC] # 118 710 # # Ksp632I*: # [CTCTTC] # 58 126 644 # # MaeI: # [CTAG] # 10 98 344 376 # # MaeII: # [ACGT] # 19 228 581 # # MboI: # [GATC] # 24 63 195 234 312 # # MboII: # [GAAGA] # 2 31 39 148 156 225 # 227 # # McrI*: # [CGRYCG] # 261 567 # # MnlI: # [CCTC] # 18 70 116 181 197 246 # # MseI: # [TTAA] # 3 61 129 141 181 313 # # MwoI: # [GCNNNNNNNGC] # 150 335 343 # # NarI: # [GGCGCC] # 233 595 # # NlaIII: # [CATG] # 114 714 # # NlaIV: # [GGNNCC] # 116 118 594 # # NspBII*: # [CMGCKG] # 358 470 # # PsrI: # [GAACNNNNNNTAC] # 32 66 730 # # PstI: # [CTGCAG] # 90 738 # # PvuI: # [CGATCG] # 261 567 # # PvuII: # [CAGCTG] # 358 470 # # RsaI: # [GTAC] # 60 116 248 404 # # ScrFI: # [CCNGG] # 36 199 593 # # SecI*: # [CCNNGG] # 36 201 591 # # SetI: # [ASST] # 6 13 37 38 39 44 # 66 101 120 124 240 # # SfaNI: # [GCATC] # 168 660 # # SfeI*: # [CTRYAG] # 86 742 # # TaiI: # [ACGT] # 19 225 584 # # TatI: # [WGTACW] # 310 518 # # TfiI: # [GAWTC] # 60 71 338 359 # # TseI: # [GCWGC] # 62 88 678 # # TsoI: # [TARCCA] # 16 812 # # Tsp4CI*: # [ACNGT] # 87 741 # # TspDTI: # [ATGAA] # 91 320 417 # # TspEI: # [AATT] # 40 45 70 71 178 424 # # TspGWI: # [ACGGA] # 351 477 # # TspRI: # [CASTGNN] # 324 504 # # TstI: # [CACNNNNNNTCC] # 32 71 725 # # VspI: # [ATTAAT] # 181 647 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 828 # Reported_sequences: 1 # Reported_hitcount: 174 #---------------------------------------