Restriction Fragments for RFA1/YAR007C



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The currently selected gene/sequence is :

RFA1/YAR007C on chromosome I from coordinates 158619 to 156754.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.16761.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + BtsI GCAGTG 12 10 . . 6 12 + TspRI CASTGNN 12 3 . . 19 22 + TaqI TCGA 19 21 . . 21 24 - MnlI CCTC 14 13 . . 32 36 - TspDTI ATGAA 22 20 . . 38 42 + SfaNI GCATC 47 51 . . 41 45 - MboII GAAGA 33 32 . . 44 48 - HphI GGTGA 36 35 . . 58 61 + SetI ASST 61 57 . . 60 63 + Csp6I GTAC 60 62 . . 60 63 + RsaI GTAC 61 61 . . 70 80 + BsiYI* CCNNNNNNNGG 76 73 . . 72 79 + SgrAI CRCCGGYG 73 77 . . 73 78 + Cfr10I RCCGGY 73 77 . . 73 78 + BetI* WCCGGW 73 77 . . 73 78 + AgeI ACCGGT 73 77 . . 74 77 + HpaII CCGG 74 76 . . 93 98 + PsiI TTATAA 95 95 . . 101 105 + BssKI CCNGG 100 105 . . 101 105 + BseBI CCWGG 102 103 . . 101 105 + ScrFI CCNGG 102 103 . . 101 105 + EcoRII CCWGG 100 105 . . 109 113 + Hpy188I TCNGA 111 110 . . 112 116 - BccI CCATC 106 107 . . 117 120 + CviJI RGCY 118 118 . . 135 140 + ApoI RAATTY 135 139 . . 136 139 + TspEI AATT 135 139 . . 140 145 + BclI TGATCA 140 144 . . 141 144 + DpnI GATC 142 142 . . 141 144 + BstKTI GATC 143 141 . . 141 144 + MboI GATC 140 144 . . 143 148 + Hpy178III* TCNNGA 144 146 . . 143 148 + BspHI TCATGA 143 147 . . 144 147 + FatI CATG 143 147 . . 144 147 + NlaIII CATG 147 143 . . 144 147 + CviAII CATG 144 146 . . 151 155 + Hpy188I TCNGA 153 152 . . 154 158 - BccI CCATC 148 149 . . 165 175 + BsiYI* CCNNNNNNNGG 171 168 . . 166 171 + NdeI CATATG 167 169 . . 169 173 + TspDTI ATGAA 184 182 . . 174 177 + CviJI RGCY 175 175 . . 171 175 - Hin4II* CCTTC 165 164 . . 192 195 + SetI ASST 195 191 . . 192 195 + CviJI RGCY 193 193 . . 192 195 + AluI AGCT 193 193 . . 193 197 + BlsI GCNGC 195 194 . . 193 197 + BisI GCNGC 194 195 . . 193 197 + TseI GCWGC 193 196 . . 195 198 + CviRI* TGCA 196 196 . . 196 200 + SfaNI GCATC 205 209 . . 193 197 - BbvI GCAGC 180 184 . . 197 201 - FokI GGATG 183 187 . . 197 201 - BseGI GGATG 196 194 . . 207 217 + BsiYI* CCNNNNNNNGG 213 210 . . 207 217 + PflMI CCANNNNNTGG 213 210 . . 207 211 - BsrI ACTGG 207 205 . . 229 233 + HphI GGTGA 241 240 . . 232 237 + EcoRV GATATC 234 234 . . 237 246 + MslI CAYNNNNRTG 241 241 . . 241 244 + FnuDII* CGCG 242 242 . . 249 252 + TspEI AATT 248 252 . . 252 255 + CviRI* TGCA 253 253 . . 253 263 + MwoI GCNNNNNNNGC 259 256 . . 253 263 + BstAPI GCANNNNNTGC 259 256 . . 254 262 + AlwNI CAGNNNCTG 259 256 . . 258 261 + SetI ASST 261 257 . . 258 263 + BspMI ACCTGC 267 271 . . 287 290 + TaiI ACGT 290 286 . . 287 290 + SetI ASST 290 286 . . 287 290 + MaeII ACGT 287 289 . . 315 319 + BmgT120I GGNCC 316 317 . . 315 319 + AvaII GGWCC 315 318 . . 315 319 + AsuI* GGNCC 315 318 . . 318 322 - BsrI ACTGG 318 316 . . 323 326 + FnuDII* CGCG 324 324 . . 324 329 + Cac8I GCNNGC 326 326 . . 337 342 + Hpy166II GTNNAC 339 339 . . 337 342 + HindII GTYRAC 339 339 . . 346 351 + SpeI ACTAGT 346 350 . . 347 350 + MaeI CTAG 347 349 . . 349 354 + TatI WGTACW 349 353 . . 349 354 + ScaI AGTACT 351 351 . . 350 353 + Csp6I GTAC 350 352 . . 350 353 + RsaI GTAC 351 351 . . 372 376 + DdeI CTNAG 372 375 . . 372 376 + BspCNI CTCAG 385 383 . . 372 376 + BseMII CTCAG 386 384 . . 373 377 + Hpy188I TCNGA 375 374 . . 378 382 + SfaNI GCATC 387 391 . . 379 388 + MslI CAYNNNNRTG 383 383 . . 379 383 - FokI GGATG 365 369 . . 379 383 - BseGI GGATG 378 376 . . 386 390 + TspDTI ATGAA 401 399 . . 391 394 + SetI ASST 394 390 . . 394 397 + MseI TTAA 394 396 . . 403 407 + MboII GAAGA 415 414 . . 416 420 + Tsp4CI* ACNGT 418 417 . . 417 423 + TspRI CASTGNN 423 414 . . 445 455 + MwoI GCNNNNNNNGC 451 448 . . 445 450 + Cac8I GCNNGC 447 447 . . 449 454 + BsrDI GCAATG 456 454 . . 460 464 - BslFI GGGAC 445 449 . . 471 475 + BlsI GCNGC 473 472 . . 471 475 + BisI GCNGC 472 473 . . 471 475 + TseI GCWGC 471 474 . . 473 476 + CviRI* TGCA 474 474 . . 471 475 - BbvI GCAGC 458 462 . . 474 479 - BsmI GAATGC 474 472 . . 492 497 + BsmI GAATGC 498 496 . . 495 498 + CviRI* TGCA 496 496 . . 508 513 + ApoI RAATTY 508 512 . . 509 512 + TspEI AATT 508 512 . . 518 522 + TspDTI ATGAA 533 531 . . 529 532 + TspEI AATT 528 532 . . 549 552 + TspEI AATT 548 552 . . 557 561 + BccI CCATC 565 566 . . 560 563 + TaqI TCGA 560 562 . . 567 571 + Tsp4CI* ACNGT 569 568 . . 570 574 + BsmAI GTCTC 575 579 . . 583 588 + AclI AACGTT 584 586 . . 584 587 + TaiI ACGT 587 583 . . 584 587 + SetI ASST 587 583 . . 584 587 + MaeII ACGT 584 586 . . 624 627 + TspEI AATT 623 627 . . 626 629 + MseI TTAA 626 628 . . 631 634 + TaiI ACGT 634 630 . . 631 634 + SetI ASST 634 630 . . 631 634 + MaeII ACGT 631 633 . . 648 651 + SetI ASST 651 647 . . 649 653 + HphI GGTGA 661 660 . . 647 650 - MnlI CCTC 640 639 . . 656 661 + Hpy166II GTNNAC 658 658 . . 652 656 - BccI CCATC 646 647 . . 670 675 + Hpy166II GTNNAC 672 672 . . 670 675 + HindII GTYRAC 672 672 . . 685 688 + SetI ASST 688 684 . . 686 689 + MnlI CCTC 696 695 . . 681 686 - BciVI GTATCC 675 674 . . 688 693 + Hpy178III* TCNNGA 689 691 . . 689 694 + Eco57MI CTGRAG 710 708 . . 689 694 + GsuI CTGGAG 710 708 . . 698 702 + Hpy188I TCNGA 700 699 . . 699 709 + BsiYI* CCNNNNNNNGG 705 702 . . 702 705 + CviJI RGCY 703 703 . . 704 709 + DsaI* CCRYGG 704 708 . . 704 709 + SecI* CCNNGG 704 708 . . 706 710 + BceAI ACGGC 722 724 . . 713 716 + MseI TTAA 713 715 . . 730 735 + ApoI RAATTY 730 734 . . 731 734 + TspEI AATT 730 734 . . 734 737 + MseI TTAA 734 736 . . 740 745 + ApoI RAATTY 740 744 . . 741 744 + TspEI AATT 740 744 . . 751 755 - Hin4II* CCTTC 745 744 . . 760 765 + Hpy166II GTNNAC 762 762 . . 760 765 + BssNAI GTATAC 762 762 . . 760 765 + AccI GTMKAC 761 763 . . 785 790 + MmeI TCCRAC 810 808 . . 792 795 + SetI ASST 795 791 . . 792 795 + CviJI RGCY 793 793 . . 792 795 + AluI AGCT 793 793 . . 793 799 + EspI* GCTNAGC 794 797 . . 794 798 + DdeI CTNAG 794 797 . . 797 800 + CviJI RGCY 798 798 . . 803 806 + TspEI AATT 802 806 . . 820 829 + MslI CAYNNNNRTG 824 824 . . 827 831 + TspDTI ATGAA 842 840 . . 834 839 + ApoI RAATTY 834 838 . . 835 838 + TspEI AATT 834 838 . . 849 855 + TspRI CASTGNN 855 846 . . 845 849 - BsmAI GTCTC 839 843 . . 850 854 + Tsp4CI* ACNGT 852 851 . . 859 863 + MboII GAAGA 871 870 . . 869 872 + TaqI TCGA 869 871 . . 872 876 + TspDTI ATGAA 887 885 . . 885 889 + Hpy188I TCNGA 887 886 . . 901 904 + TspEI AATT 900 904 . . 904 908 - TspDTI ATGAA 894 892 . . 913 916 + MaeI CTAG 913 915 . . 916 920 - SfaNI GCATC 906 910 . . 924 928 + Hpy188I TCNGA 926 925 . . 930 934 + BssKI CCNGG 929 934 . . 930 934 + BseBI CCWGG 931 932 . . 930 934 + ScrFI CCNGG 931 932 . . 930 934 + EcoRII CCWGG 929 934 . . 939 944 + ApoI RAATTY 939 943 . . 940 943 + TspEI AATT 939 943 . . 943 948 + MmeI TCCRAC 968 966 . . 947 950 + TaiI ACGT 950 946 . . 947 950 + SetI ASST 950 946 . . 947 950 + MaeII ACGT 947 949 . . 949 954 + Hpy166II GTNNAC 951 951 . . 949 954 + AccI GTMKAC 950 952 . . 952 957 + ZraI GACGTC 954 954 . . 952 957 + AcyI GRCGYC 953 955 . . 952 957 + AatII GACGTC 956 952 . . 953 956 + TaiI ACGT 956 952 . . 953 956 + SetI ASST 956 952 . . 953 956 + MaeII ACGT 953 955 . . 957 960 + MnlI CCTC 967 966 . . 957 962 + SecI* CCNNGG 957 961 . . 992 995 + SetI ASST 995 991 . . 992 995 + CviJI RGCY 993 993 . . 992 995 + AluI AGCT 993 993 . . 1005 1008 + CviJI RGCY 1006 1006 . . 1011 1015 + MboII GAAGA 1023 1022 . . 1006 1011 - GsaI CCCAGC 1010 1006 . . 1006 1011 - BseYI CCCAGC 1006 1010 . . 1011 1020 + XmnI GAANNNNTTC 1015 1015 . . 1015 1020 + ApoI RAATTY 1015 1019 . . 1016 1019 + TspEI AATT 1015 1019 . . 1019 1022 + TaqI TCGA 1019 1021 . . 1020 1025 + McrI* CGRYCG 1023 1021 . . 1020 1025 + PvuI CGATCG 1023 1021 . . 1021 1024 + DpnI GATC 1022 1022 . . 1021 1024 + BstKTI GATC 1023 1021 . . 1021 1024 + MboI GATC 1020 1024 . . 1024 1028 + Hpy99I CGWCG 1028 1023 . . 1026 1031 + Hpy178III* TCNNGA 1027 1029 . . 1028 1032 + Tsp45I GTSAC 1027 1032 . . 1028 1032 + MaeIII GTNAC 1027 1032 . . 1037 1042 + MfeI CAATTG 1037 1041 . . 1038 1041 + TspEI AATT 1037 1041 . . 1042 1047 + Hpy166II GTNNAC 1044 1044 . . 1042 1047 + HindII GTYRAC 1044 1044 . . 1048 1052 + HinfI GANTC 1048 1051 . . 1048 1052 - PleI GAGTC 1042 1043 . . 1048 1052 - MlyI GAGTC 1042 1042 . . 1072 1075 + CviJI RGCY 1073 1073 . . 1072 1075 + HaeIII GGCC 1073 1073 . . 1080 1084 + TfiI GAWTC 1080 1083 . . 1080 1084 + HinfI GANTC 1080 1083 . . 1086 1091 + Cac8I GCNNGC 1088 1088 . . 1089 1092 + CviJI RGCY 1090 1090 . . 1103 1106 + SetI ASST 1106 1102 . . 1104 1108 + Hin4II* CCTTC 1114 1113 . . 1107 1112 + Hpy178III* TCNNGA 1108 1110 . . 1109 1114 + Eco57MI CTGRAG 1130 1128 . . 1109 1114 + Eco57I CTGAAG 1130 1128 . . 1113 1116 + SetI ASST 1116 1112 . . 1111 1115 - Hin4II* CCTTC 1105 1104 . . 1123 1127 + BlsI GCNGC 1125 1124 . . 1123 1127 + BisI GCNGC 1124 1125 . . 1123 1127 + TseI GCWGC 1123 1126 . . 1123 1127 - BbvI GCAGC 1110 1114 . . 1130 1133 + MseI TTAA 1130 1132 . . 1134 1137 + SetI ASST 1137 1133 . . 1144 1148 + Tsp45I GTSAC 1143 1148 . . 1144 1148 + MaeIII GTNAC 1143 1148 . . 1147 1151 + TspGWI ACGGA 1162 1160 . . 1184 1187 + MaeI CTAG 1184 1186 . . 1187 1190 + Csp6I GTAC 1187 1189 . . 1187 1190 + RsaI GTAC 1188 1188 . . 1194 1198 + TfiI GAWTC 1194 1197 . . 1194 1198 + HinfI GANTC 1194 1197 . . 1194 1204 + Hin4I GAYNNNNNVTC 1185 1180 1217 1212 1197 1201 + Hpy188I TCNGA 1199 1198 . . 1200 1204 + TfiI GAWTC 1200 1203 . . 1200 1204 + HinfI GANTC 1200 1203 . . 1203 1208 + Hpy178III* TCNNGA 1204 1206 . . 1194 1204 - Hin4I GAYNNNNNVTC 1217 1212 1185 1180 1208 1213 + ApoI RAATTY 1208 1212 . . 1209 1212 + TspEI AATT 1208 1212 . . 1212 1217 + Hpy178III* TCNNGA 1213 1215 . . 1213 1219 + SauI* CCTNAGG 1214 1217 . . 1214 1218 + DdeI CTNAG 1214 1217 . . 1214 1218 - BspCNI CTCAG 1206 1204 . . 1214 1218 - BseMII CTCAG 1205 1203 . . 1216 1219 - MnlI CCTC 1209 1208 . . 1220 1225 + NdeI CATATG 1221 1223 . . 1228 1231 + MseI TTAA 1228 1230 . . 1243 1247 + TfiI GAWTC 1243 1246 . . 1243 1247 + HinfI GANTC 1243 1246 . . 1247 1252 + StyI CCWWGG 1247 1251 . . 1247 1252 + SecI* CCNNGG 1247 1251 . . 1251 1255 + BmgT120I GGNCC 1252 1253 . . 1251 1255 + AsuI* GGNCC 1251 1254 . . 1252 1255 + CviJI RGCY 1253 1253 . . 1252 1255 + HaeIII GGCC 1253 1253 . . 1253 1257 + TauI GCSGC 1256 1253 . . 1253 1257 + BlsI GCNGC 1255 1254 . . 1253 1257 + BisI GCNGC 1254 1255 . . 1254 1257 + AciI CCGC 1254 1256 . . 1267 1271 - TspDTI ATGAA 1257 1255 . . 1275 1280 + AhaIII* TTTAAA 1277 1277 . . 1276 1279 + MseI TTAA 1276 1278 . . 1288 1292 + BssKI CCNGG 1287 1292 . . 1288 1292 + ScrFI CCNGG 1289 1290 . . 1288 1292 + CauII* CCSGG 1289 1290 . . 1288 1298 + BsiYI* CCNNNNNNNGG 1294 1291 . . 1289 1292 + HpaII CCGG 1289 1291 . . 1295 1300 - TaqII CACCCA 1285 1283 . . 1308 1311 + CviJI RGCY 1309 1309 . . 1309 1313 + BlsI GCNGC 1311 1310 . . 1309 1313 + BisI GCNGC 1310 1311 . . 1309 1313 + TseI GCWGC 1309 1312 . . 1312 1317 + Cac8I GCNNGC 1314 1314 . . 1312 1317 + BmtI GCTAGC 1316 1312 . . 1312 1317 + NheI GCTAGC 1312 1316 . . 1313 1316 + MaeI CTAG 1313 1315 . . 1309 1313 - BbvI GCAGC 1296 1300 . . 1315 1318 + SetI ASST 1318 1314 . . 1315 1318 + CviJI RGCY 1316 1316 . . 1315 1318 + AluI AGCT 1316 1316 . . 1318 1321 + MseI TTAA 1318 1320 . . 1324 1329 + ApoI RAATTY 1324 1328 . . 1325 1328 + TspEI AATT 1324 1328 . . 1327 1331 - TspDTI ATGAA 1317 1315 . . 1333 1339 + EspI* GCTNAGC 1334 1337 . . 1334 1338 + DdeI CTNAG 1334 1337 . . 1334 1338 + BspCNI CTCAG 1347 1345 . . 1334 1338 + BseMII CTCAG 1348 1346 . . 1329 1334 - BsrDI GCAATG 1328 1326 . . 1352 1355 + MaeI CTAG 1352 1354 . . 1355 1360 + SacI GAGCTC 1359 1355 . . 1355 1360 + Ecl136II GAGCTC 1357 1357 . . 1355 1360 + SduI GDGCHC 1359 1355 . . 1355 1360 + HgiJII* GRGCYC 1359 1355 . . 1355 1360 + HgiAI* GWGCWC 1359 1355 . . 1356 1359 + SetI ASST 1359 1355 . . 1356 1359 + CviJI RGCY 1357 1357 . . 1356 1359 + AluI AGCT 1357 1357 . . 1358 1363 + SmlI CTYRAG 1358 1362 . . 1362 1365 + SetI ASST 1365 1361 . . 1362 1365 + CviJI RGCY 1363 1363 . . 1362 1365 + AluI AGCT 1363 1363 . . 1358 1363 - Bce83I* CTTGAG 1343 1341 . . 1372 1375 + MaeI CTAG 1372 1374 . . 1376 1380 + MboII GAAGA 1388 1387 . . 1389 1392 + SetI ASST 1392 1388 . . 1390 1394 + HphI GGTGA 1402 1401 . . 1391 1395 + Tsp45I GTSAC 1390 1395 . . 1391 1395 + MaeIII GTNAC 1390 1395 . . 1406 1409 + MseI TTAA 1406 1408 . . 1410 1413 + SetI ASST 1413 1409 . . 1410 1413 + CviJI RGCY 1411 1411 . . 1410 1413 + AluI AGCT 1411 1411 . . 1411 1415 + BlsI GCNGC 1413 1412 . . 1411 1415 + BisI GCNGC 1412 1413 . . 1411 1415 + TseI GCWGC 1411 1414 . . 1411 1415 - BbvI GCAGC 1398 1402 . . 1426 1429 + MseI TTAA 1426 1428 . . 1438 1441 + TspEI AATT 1437 1441 . . 1443 1446 + CviRI* TGCA 1444 1444 . . 1444 1454 + MwoI GCNNNNNNNGC 1450 1447 . . 1444 1454 + BstAPI GCANNNNNTGC 1450 1447 . . 1468 1471 + TspEI AATT 1467 1471 . . 1485 1490 + Hpy178III* TCNNGA 1486 1488 . . 1493 1496 + CviJI RGCY 1494 1494 . . 1502 1505 + Csp6I GTAC 1502 1504 . . 1498 1502 - BccI CCATC 1492 1493 . . 1502 1505 + RsaI GTAC 1503 1503 . . 1536 1539 + CviRI* TGCA 1537 1537 . . 1541 1544 + CviJI RGCY 1542 1542 . . 1541 1544 + HaeIII GGCC 1542 1542 . . 1546 1549 + TspEI AATT 1545 1549 . . 1559 1564 + Hpy178III* TCNNGA 1560 1562 . . 1572 1575 + TspEI AATT 1571 1575 . . 1598 1601 + CviJI RGCY 1599 1599 . . 1620 1623 + SetI ASST 1623 1619 . . 1620 1623 + CviJI RGCY 1621 1621 . . 1620 1623 + AluI AGCT 1621 1621 . . 1628 1631 + TspEI AATT 1627 1631 . . 1634 1639 - TaqII CACCCA 1624 1622 . . 1642 1646 - SfaNI GCATC 1632 1636 . . 1653 1658 + VspI ATTAAT 1654 1656 . . 1654 1657 + MseI TTAA 1654 1656 . . 1669 1673 + MboII GAAGA 1681 1680 . . 1665 1669 - Hin4II* CCTTC 1659 1658 . . 1672 1676 + MboII GAAGA 1684 1683 . . 1672 1677 + BbvII* GAAGAC 1679 1683 . . 1684 1689 + EcoRI GAATTC 1684 1688 . . 1684 1689 + ApoI RAATTY 1684 1688 . . 1685 1688 + TspEI AATT 1684 1688 . . 1695 1698 + TspEI AATT 1694 1698 . . 1706 1711 + BciVI GTATCC 1717 1716 . . 1714 1718 + TspDTI ATGAA 1729 1727 . . 1741 1744 + Hin6I GCGC 1741 1743 . . 1741 1744 + GlaI GCGC 1742 1742 . . 1741 1744 + HhaI GCGC 1743 1741 . . 1742 1745 + FnuDII* CGCG 1743 1743 . . 1747 1750 - MnlI CCTC 1740 1739 . . 1749 1754 - BciVI GTATCC 1743 1742 . . 1761 1766 + BclI TGATCA 1761 1765 . . 1762 1765 + DpnI GATC 1763 1763 . . 1762 1765 + BstKTI GATC 1764 1762 . . 1762 1765 + MboI GATC 1761 1765 . . 1773 1776 + TspEI AATT 1772 1776 . . 1783 1787 + Tsp4CI* ACNGT 1785 1784 . . 1793 1796 + SetI ASST 1796 1792 . . 1801 1804 + SetI ASST 1804 1800 . . 1801 1804 + CviJI RGCY 1802 1802 . . 1801 1804 + AluI AGCT 1802 1802 . . 1807 1810 + TspEI AATT 1806 1810 . . 1815 1818 + CviJI RGCY 1816 1816 . . 1817 1822 + Eco57MI CTGRAG 1838 1836 . . 1817 1822 + Eco57I CTGAAG 1838 1836 . . 1821 1824 + CviJI RGCY 1822 1822 . . 1824 1835 + BcgI CGANNNNNNTGC 1813 1811 1847 1845 1837 1847 + Hin4I GAYNNNNNVTC 1828 1823 1860 1855 1837 1847 - Hin4I GAYNNNNNVTC 1860 1855 1828 1823 1847 1852 + StyI CCWWGG 1847 1851 . . 1847 1852 + SecI* CCNNGG 1847 1851 . . 1851 1854 + CviJI RGCY 1852 1852 . . 1852 1862 + MwoI GCNNNNNNNGC 1858 1855 . . 1860 1863 + SetI ASST 1863 1859 . . 1860 1863 + CviJI RGCY 1861 1861 . . 1860 1863 + AluI AGCT 1861 1861 . . 1863 1866 + MseI TTAA 1863 1865 . . #--------------------------------------- # # # AatII: # [GACGTC] # 910 956 # # AccI: # [GTMKAC] # 189 761 916 # # AciI: # [CCGC] # 612 1254 # # AclI: # [AACGTT] # 584 1282 # # AcyI: # [GRCGYC] # 913 953 # # AgeI: # [ACCGGT] # 73 1793 # # AhaIII*: # [TTTAAA] # 589 1277 # # AluI: # [AGCT] # 5 6 41 48 59 181 # 193 200 210 323 600 # # AlwNI: # [CAGNNNCTG] # 259 1607 # # ApoI: # [RAATTY] # 10 76 94 105 116 135 # 182 193 222 360 373 # # AsuI*: # [GGNCC] # 315 615 936 # # AvaII: # [GGWCC] # 315 1551 # # BbvI: # [GCAGC] # 102 180 186 278 468 652 # # BbvII*: # [GAAGAC] # 187 1679 # # BccI: # [CCATC] # 42 81 106 374 417 846 # # Bce83I*: # [CTTGAG] # 523 1343 # # BceAI: # [ACGGC] # 722 1144 # # BcgI: # [CGANNNNNNTGC] # 19 34 1813 # # BciVI: # [GTATCC] # 26 123 675 1042 # # BclI: # [TGATCA] # 105 140 1621 # # BetI*: # [WCCGGW] # 73 1793 # # BisI: # [GCNGC] # 56 102 130 194 278 454 # 652 # # BlsI: # [GCNGC] # 56 102 130 195 278 453 # 652 # # BmgT120I: # [GGNCC] # 316 614 936 # # BmtI: # [GCTAGC] # 550 1316 # # BseBI: # [CCWGG] # 102 829 935 # # BseGI: # [GGATG] # 182 196 1488 # # BseMII: # [CTCAG] # 143 386 518 819 # # BseYI: # [CCCAGC] # 860 1006 # # BsiYI*: # [CCNNNNNNNGG] # 42 76 95 492 572 589 # # BslFI: # [GGGAC] # 445 1421 # # BsmAI: # [GTCTC] # 264 575 1027 # # BsmI: # [GAATGC] # 24 474 1368 # # BspCNI: # [CTCAG] # 141 385 519 821 # # BspHI: # [TCATGA] # 143 1723 # # BspMI: # [ACCTGC] # 267 1599 # # BsrDI: # [GCAATG] # 456 538 872 # # BsrI: # [ACTGG] # 111 207 1548 # # BssKI: # [CCNGG] # 100 358 579 829 # # BssNAI: # [GTATAC] # 762 1104 # # BstAPI: # [GCANNNNNTGC] # 259 416 1191 # # BstKTI: # [GATC] # 102 143 741 880 # # BtsI: # [GCAGTG] # 12 1854 # # Cac8I: # [GCNNGC] # 121 226 326 552 641 # # CauII*: # [CCSGG] # 577 1289 # # Cfr10I: # [RCCGGY] # 73 1793 # # Csp6I: # [GTAC] # 60 290 315 364 837 # # CviAII: # [CATG] # 144 1722 # # CviJI: # [RGCY] # 5 5 6 6 7 9 # 13 14 17 18 22 30 # 41 48 48 56 57 57 # 67 83 90 118 163 181 # 195 510 # # CviRI*: # [TGCA] # 22 57 93 196 221 329 # 948 # # DdeI: # [CTNAG] # 120 372 420 422 532 # # DpnI: # [GATC] # 103 142 741 880 # # DsaI*: # [CCRYGG] # 704 1162 # # Ecl136II: # [GAGCTC] # 509 1357 # # Eco57I: # [CTGAAG] # 28 708 1130 # # Eco57MI: # [CTGRAG] # 28 420 708 710 # # EcoRI: # [GAATTC] # 182 1684 # # EcoRII: # [CCWGG] # 100 829 937 # # EcoRV: # [GATATC] # 234 1632 # # EspI*: # [GCTNAGC] # 532 540 794 # # FatI: # [CATG] # 143 1723 # # FnuDII*: # [CGCG] # 82 123 242 1419 # # FokI: # [GGATG] # 182 183 1501 # # GlaI: # [GCGC] # 124 1742 # # GsaI: # [CCCAGC] # 856 1010 # # GsuI: # [CTGGAG] # 710 1156 # # HaeIII: # [GGCC] # 180 289 324 1073 # # HgiAI*: # [GWGCWC] # 507 1359 # # HgiJII*: # [GRGCYC] # 507 1359 # # HhaI: # [GCGC] # 123 1743 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 6 32 # 32 611 1185 # # Hin4II*: # [CCTTC] # 9 165 207 360 545 580 # # Hin6I: # [GCGC] # 125 1741 # # HindII: # [GTYRAC] # 333 339 372 822 # # HinfI: # [GANTC] # 6 32 43 114 623 1048 # # HpaII: # [CCGG] # 74 577 1215 # # HphI: # [GGTGA] # 36 205 420 464 741 # # Hpy166II: # [GTNNAC] # 14 90 93 189 319 339 # 822 # # Hpy178III*: # [TCNNGA] # 9 74 81 96 144 273 # 306 338 545 # # Hpy188I: # [TCNGA] # 39 42 111 187 222 273 # 325 667 # # Hpy99I: # [CGWCG] # 838 1028 # # MaeI: # [CTAG] # 20 39 129 271 347 494 # 566 # # MaeII: # [ACGT] # 6 47 287 297 316 913 # # MaeIII: # [GTNAC] # 116 247 476 1027 # # MboI: # [GATC] # 105 140 741 880 # # MboII: # [GAAGA] # 3 33 152 182 293 365 # 382 456 # # McrI*: # [CGRYCG] # 843 1023 # # MfeI: # [CAATTG] # 829 1037 # # MlyI: # [GAGTC] # 824 1042 # # MmeI: # [TCCRAC] # 158 810 898 # # MnlI: # [CCTC] # 14 56 126 242 271 531 # 626 # # MseI: # [TTAA] # 3 20 21 42 48 87 # 88 98 209 228 232 394 # 396 # # MslI: # [CAYNNNNRTG] # 142 241 441 1042 # # MwoI: # [GCNNNNNNNGC] # 8 192 259 408 999 # # NdeI: # [CATATG] # 167 645 1054 # # NheI: # [GCTAGC] # 554 1312 # # NlaIII: # [CATG] # 147 1719 # # PflMI: # [CCANNNNNTGG] # 213 1653 # # PleI: # [GAGTC] # 824 1042 # # PsiI: # [TTATAA] # 95 1771 # # PvuI: # [CGATCG] # 843 1023 # # RsaI: # [GTAC] # 61 290 315 363 837 # # SacI: # [GAGCTC] # 507 1359 # # SauI*: # [CCTNAGG] # 652 1214 # # ScaI: # [AGTACT] # 351 1515 # # ScrFI: # [CCNGG] # 102 358 577 829 # # SduI: # [GDGCHC] # 507 1359 # # SecI*: # [CCNNGG] # 19 253 290 600 704 # # SetI: # [ASST] # 3 6 6 8 10 17 # 21 21 27 29 37 39 # 41 47 59 61 66 104 # 107 111 134 155 173 181 # 193 210 # # SfaNI: # [GCATC] # 47 158 182 234 519 726 # # SgrAI: # [CRCCGGYG] # 73 1793 # # SmlI: # [CTYRAG] # 508 1358 # # SpeI: # [ACTAGT] # 346 1520 # # StyI: # [CCWWGG] # 19 600 1247 # # TaiI: # [ACGT] # 6 47 290 297 316 910 # # TaqI: # [TCGA] # 19 150 309 541 847 # # TaqII: # [CACCCA] # 242 339 1285 # # TatI: # [WGTACW] # 349 1517 # # TauI: # [GCSGC] # 610 1256 # # TfiI: # [GAWTC] # 6 43 114 623 1080 # # TseI: # [GCWGC] # 102 186 193 278 455 652 # # Tsp45I: # [GTSAC] # 116 247 476 1027 # # Tsp4CI*: # [ACNGT] # 81 151 283 418 933 # # TspDTI: # [ATGAA] # 7 22 45 60 132 137 # 162 217 309 363 412 # # TspEI: # [AATT] # 10 10 20 20 22 26 # 30 32 34 39 56 57 # 60 62 66 75 76 78 # 78 107 113 113 116 135 # 171 260 # # TspGWI: # [ACGGA] # 704 1162 # # TspRI: # [CASTGNN] # 12 411 432 1011 # # VspI: # [ATTAAT] # 212 1654 # # XmnI: # [GAANNNNTTC] # 851 1015 # # ZraI: # [GACGTC] # 912 954 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1866 # Reported_sequences: 1 # Reported_hitcount: 410 #---------------------------------------