Restriction Fragments for FLC2/YAL053W



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

FLC2/YAL053W on chromosome I from coordinates 45899 to 48250.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.12119.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + DpnI GATC 4 4 . . 3 6 + BstKTI GATC 5 3 . . 3 6 + MboI GATC 2 6 . . 16 19 + SetI ASST 19 15 . . 17 21 + Hin4II* CCTTC 27 26 . . 17 27 + EcoNI CCTNNNNNAGG 21 22 . . 17 27 + BsiYI* CCNNNNNNNGG 23 20 . . 25 28 + SetI ASST 28 24 . . 26 31 + NlaIV GGNNCC 28 28 . . 26 31 + HgiCI* GGYRCC 26 30 . . 34 37 + MseI TTAA 34 36 . . 37 40 + TaiI ACGT 40 36 . . 37 40 + SetI ASST 40 36 . . 37 40 + MaeII ACGT 37 39 . . 37 42 + AflIII ACRYGT 37 41 . . 46 49 + Csp6I GTAC 46 48 . . 46 49 + RsaI GTAC 47 47 . . 48 52 + Tsp4CI* ACNGT 50 49 . . 51 56 + BsgI GTGCAG 72 70 . . 52 55 + CviRI* TGCA 53 53 . . 53 57 + BlsI GCNGC 55 54 . . 53 57 + BisI GCNGC 54 55 . . 53 57 + BbvI GCAGC 65 69 . . 53 57 + TseI GCWGC 53 56 . . 54 59 + NspBII* CMGCKG 56 56 . . 59 62 + Csp6I GTAC 59 61 . . 59 62 + RsaI GTAC 60 60 . . 56 59 - AciI CCGC 56 58 . . 66 69 + TaiI ACGT 69 65 . . 66 69 + SetI ASST 69 65 . . 66 69 + MaeII ACGT 66 68 . . 59 71 - PsrI GAACNNNNNNTAC 83 78 51 46 71 74 + MnlI CCTC 81 80 . . 73 77 + Hpy188I TCNGA 75 74 . . 94 97 + HpaII CCGG 94 96 . . 96 101 + AcyI GRCGYC 97 99 . . 97 101 - HgaI GACGC 86 91 . . 102 105 + CviRI* TGCA 103 103 . . 113 116 + CviRI* TGCA 114 114 . . 133 136 + TaiI ACGT 136 132 . . 133 136 + SetI ASST 136 132 . . 133 136 + MaeII ACGT 133 135 . . 133 138 + AflIII ACRYGT 133 137 . . 132 137 - BtrI CACGTC 134 134 . . 145 148 + TspEI AATT 144 148 . . 152 155 + TspEI AATT 151 155 . . 154 157 + MseI TTAA 154 156 . . 157 161 + BceAI ACGGC 173 175 . . 160 164 + SfaNI GCATC 169 173 . . 178 183 + ApoI RAATTY 178 182 . . 179 182 + TspEI AATT 178 182 . . 199 203 + Tsp4CI* ACNGT 201 200 . . 217 221 + HgaI GACGC 226 231 . . 224 228 + Hpy99I CGWCG 228 223 . . 227 231 + Hpy99I CGWCG 231 226 . . 228 232 + HgaI GACGC 237 242 . . 233 236 + MseI TTAA 233 235 . . 242 245 + TaiI ACGT 245 241 . . 242 245 + SetI ASST 245 241 . . 242 245 + MaeII ACGT 242 244 . . 244 248 + Tsp45I GTSAC 243 248 . . 244 248 + MaeIII GTNAC 243 248 . . 246 252 + TspRI CASTGNN 252 243 . . 247 251 + Tsp4CI* ACNGT 249 248 . . 255 258 + CviJI RGCY 256 256 . . 252 256 - Hin4II* CCTTC 246 245 . . 256 262 + EspI* GCTNAGC 257 260 . . 257 261 + DdeI CTNAG 257 260 . . 260 263 + SetI ASST 263 259 . . 260 263 + CviJI RGCY 261 261 . . 260 263 + AluI AGCT 261 261 . . 257 261 - BspCNI CTCAG 249 247 . . 257 261 - BseMII CTCAG 248 246 . . 261 265 + BlsI GCNGC 263 262 . . 261 265 + BisI GCNGC 262 263 . . 261 265 + TseI GCWGC 261 264 . . 261 265 - BbvI GCAGC 248 252 . . 272 276 + TspGWI ACGGA 287 285 . . 275 285 + Hin4I GAYNNNNNVTC 266 261 298 293 275 285 + Eam1105I GACNNNNNGTC 280 279 . . 284 290 + PfoI TCCNGGA 284 289 . . 275 285 - Hin4I GAYNNNNNVTC 298 293 266 261 285 289 + BssKI CCNGG 284 289 . . 285 289 + BseBI CCWGG 286 287 . . 285 289 + ScrFI CCNGG 286 287 . . 285 289 + EcoRII CCWGG 284 289 . . 311 316 + StyI CCWWGG 311 315 . . 311 316 + SecI* CCNNGG 311 315 . . 315 319 + BmgT120I GGNCC 316 317 . . 315 319 + AsuI* GGNCC 315 318 . . 316 319 + CviJI RGCY 317 317 . . 316 319 + HaeIII GGCC 317 317 . . 320 330 + FalI AAGNNNNNCTT 311 306 343 338 320 330 - FalI AAGNNNNNCTT 343 338 311 306 337 341 + DdeI CTNAG 337 340 . . 343 348 - GsaI CCCAGC 347 343 . . 343 348 - BseYI CCCAGC 343 347 . . 355 365 + Hin4I GAYNNNNNVTC 346 341 378 373 360 363 + FatI CATG 359 363 . . 360 363 + NlaIII CATG 363 359 . . 360 363 + CviAII CATG 360 362 . . 363 368 + Hpy166II GTNNAC 365 365 . . 355 365 - Hin4I GAYNNNNNVTC 378 373 346 341 366 375 + MslI CAYNNNNRTG 370 370 . . 366 375 + OliI CACNNNNGTG 370 370 . . 371 374 + SetI ASST 374 370 . . 372 376 + HphI GGTGA 384 383 . . 375 378 + DpnI GATC 376 376 . . 375 378 + BstKTI GATC 377 375 . . 375 378 + MboI GATC 374 378 . . 377 380 + TaqI TCGA 377 379 . . 379 383 + TfiI GAWTC 379 382 . . 379 383 + HinfI GANTC 379 382 . . 383 387 - FokI GGATG 369 373 . . 383 387 - BseGI GGATG 382 380 . . 398 401 + TspEI AATT 397 401 . . 403 407 + BssKI CCNGG 402 407 . . 403 407 + ScrFI CCNGG 404 405 . . 403 407 + CauII* CCSGG 404 405 . . 404 407 + HpaII CCGG 404 406 . . 408 413 - BsrDI GCAATG 407 405 . . 419 433 + XcmI CCANNNNNNNNNTGG 426 425 . . 423 428 + Hpy178III* TCNNGA 424 426 . . 433 437 + HgaI GACGC 442 447 . . 442 445 + Csp6I GTAC 442 444 . . 442 445 + RsaI GTAC 443 443 . . 443 448 + BsaAI YACGTR 445 445 . . 444 447 + TaiI ACGT 447 443 . . 444 447 + SetI ASST 447 443 . . 444 447 + MaeII ACGT 444 446 . . 444 449 + AflIII ACRYGT 444 448 . . 461 465 + DdeI CTNAG 461 464 . . 461 465 + BspCNI CTCAG 474 472 . . 461 465 + BseMII CTCAG 475 473 . . 462 466 + Hpy188I TCNGA 464 463 . . 472 476 + TspGWI ACGGA 487 485 . . 475 480 + EcoRI GAATTC 475 479 . . 475 480 + ApoI RAATTY 475 479 . . 476 479 + TspEI AATT 475 479 . . 478 483 + AsuII TTCGAA 479 481 . . 479 482 + TaqI TCGA 479 481 . . 487 492 + NspBII* CMGCKG 489 489 . . 487 490 + AciI CCGC 487 489 . . 489 494 + Cac8I GCNNGC 491 491 . . 492 495 + CviJI RGCY 493 493 . . 501 505 + BssKI CCNGG 500 505 . . 501 505 + BseBI CCWGG 502 503 . . 501 505 + ScrFI CCNGG 502 503 . . 501 505 + EcoRII CCWGG 500 505 . . 504 507 + CviJI RGCY 505 505 . . 509 514 + Hpy178III* TCNNGA 510 512 . . 510 515 + SmlI CTYRAG 510 514 . . 510 515 + Bce83I* CTTGAG 531 529 . . 515 519 + MaeIII GTNAC 514 519 . . 522 526 + MboII GAAGA 534 533 . . 522 527 + BbvII* GAAGAC 529 533 . . 526 530 + Tsp4CI* ACNGT 528 527 . . 527 533 + TspRI CASTGNN 533 524 . . 530 533 + CviRI* TGCA 531 531 . . 544 548 + TauI GCSGC 547 544 . . 544 548 + BlsI GCNGC 546 545 . . 544 548 + BisI GCNGC 545 546 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + HaeIII GGCC 547 547 . . 544 547 - AciI CCGC 544 546 . . 548 552 + BssKI CCNGG 547 552 . . 548 552 + BseBI CCWGG 549 550 . . 548 552 + ScrFI CCNGG 549 550 . . 548 552 + EcoRII CCWGG 547 552 . . 551 554 + CviJI RGCY 552 552 . . 551 554 + HaeIII GGCC 552 552 . . 551 555 + BmgT120I GGNCC 552 553 . . 551 555 + AsuI* GGNCC 551 554 . . 559 563 + TauI GCSGC 562 559 . . 559 563 + BlsI GCNGC 561 560 . . 559 563 + BisI GCNGC 560 561 . . 555 560 - BsrDI GCAATG 554 552 . . 560 563 + AciI CCGC 560 562 . . 567 571 + DdeI CTNAG 567 570 . . 567 571 + BspCNI CTCAG 580 578 . . 567 571 + BseMII CTCAG 581 579 . . 570 573 + SetI ASST 573 569 . . 578 583 + Hpy166II GTNNAC 580 580 . . 579 584 + TatI WGTACW 579 583 . . 580 583 + Csp6I GTAC 580 582 . . 580 583 + RsaI GTAC 581 581 . . 586 589 + SetI ASST 589 585 . . 587 590 + MnlI CCTC 597 596 . . 587 593 + SauI* CCTNAGG 588 591 . . 588 592 + DdeI CTNAG 588 591 . . 588 592 + BspCNI CTCAG 601 599 . . 588 592 + BseMII CTCAG 602 600 . . 606 609 + DpnI GATC 607 607 . . 606 609 + BstKTI GATC 608 606 . . 606 609 + MboI GATC 605 609 . . 611 615 + MaeIII GTNAC 610 615 . . 615 619 + DdeI CTNAG 615 618 . . 615 619 + BspCNI CTCAG 628 626 . . 615 619 + BseMII CTCAG 629 627 . . 617 625 + AlwNI CAGNNNCTG 622 619 . . 618 621 + CviJI RGCY 619 619 . . 619 629 + MwoI GCNNNNNNNGC 625 622 . . 621 627 + TspRI CASTGNN 627 618 . . 625 629 + BlsI GCNGC 627 626 . . 625 629 + BisI GCNGC 626 627 . . 625 629 + TseI GCWGC 625 628 . . 621 626 - BtsI GCAGTG 620 618 . . 623 628 - EcoP15I CAGCAG 595 597 . . 628 638 + MwoI GCNNNNNNNGC 634 631 . . 625 629 - BbvI GCAGC 612 616 . . 631 642 + TstI CACNNNNNNTCC 622 617 654 649 633 638 - BsrDI GCAATG 632 630 . . 640 645 + MmeI TCCRAC 665 663 . . 637 641 - HgaI GACGC 626 631 . . 647 651 + BccI CCATC 655 656 . . 658 662 - TspDTI ATGAA 648 646 . . 676 679 + MaeI CTAG 676 678 . . 678 681 + SetI ASST 681 677 . . 678 681 + CviJI RGCY 679 679 . . 678 681 + AluI AGCT 679 679 . . 679 689 + MwoI GCNNNNNNNGC 685 682 . . 684 690 + TspRI CASTGNN 690 681 . . 684 693 + MslI CAYNNNNRTG 688 688 . . 687 690 + CviRI* TGCA 688 688 . . 688 693 + BsrDI GCAATG 695 693 . . 684 689 - BtsI GCAGTG 683 681 . . 693 697 - BccI CCATC 687 688 . . 695 700 - TaqII CACCCA 685 683 . . 700 704 + BsmAI GTCTC 705 709 . . 702 707 + SmlI CTYRAG 702 706 . . 702 707 - Bce83I* CTTGAG 687 685 . . 708 713 + NlaIV GGNNCC 710 710 . . 713 718 + TaqII CACCCA 729 727 . . 721 725 + BlsI GCNGC 723 722 . . 721 725 + BisI GCNGC 722 723 . . 721 725 + TseI GCWGC 721 724 . . 717 722 - BsrDI GCAATG 716 714 . . 724 728 + TauI GCSGC 727 724 . . 724 728 + BlsI GCNGC 726 725 . . 724 728 + BisI GCNGC 725 726 . . 721 725 - BbvI GCAGC 708 712 . . 725 728 + AciI CCGC 725 727 . . 726 729 + FnuDII* CGCG 727 727 . . 729 734 + Hpy166II GTNNAC 731 731 . . 732 736 + HgaI GACGC 741 746 . . 738 743 + ApoI RAATTY 738 742 . . 739 742 + TspEI AATT 738 742 . . 749 753 + BmgT120I GGNCC 750 751 . . 749 753 + AvaII GGWCC 749 752 . . 749 753 + AsuI* GGNCC 749 752 . . 752 757 + DsaI* CCRYGG 752 756 . . 752 757 + StyI CCWWGG 752 756 . . 752 757 + NcoI CCATGG 752 756 . . 752 757 + SecI* CCNNGG 752 756 . . 753 756 + FatI CATG 752 756 . . 753 756 + NlaIII CATG 756 752 . . 753 756 + CviAII CATG 753 755 . . 777 780 + FatI CATG 776 780 . . 777 780 + NlaIII CATG 780 776 . . 777 780 + CviAII CATG 777 779 . . 775 779 - TspDTI ATGAA 765 763 . . 779 782 + CviRI* TGCA 780 780 . . 798 801 + Csp6I GTAC 798 800 . . 798 801 + RsaI GTAC 799 799 . . 799 804 + SnaBI TACGTA 801 801 . . 799 804 + BsaAI YACGTR 801 801 . . 800 803 + TaiI ACGT 803 799 . . 800 803 + SetI ASST 803 799 . . 800 803 + MaeII ACGT 800 802 . . 802 805 + Csp6I GTAC 802 804 . . 802 805 + RsaI GTAC 803 803 . . 807 810 + CviJI RGCY 808 808 . . 807 810 + HaeIII GGCC 808 808 . . 820 824 + BsmAI GTCTC 825 829 . . 824 835 + CspCI CAANNNNNGTGG 812 810 847 845 837 840 + SetI ASST 840 836 . . 837 840 + CviJI RGCY 838 838 . . 837 840 + AluI AGCT 838 838 . . 844 854 + FalI AAGNNNNNCTT 835 830 867 862 847 852 + ZraI GACGTC 849 849 . . 847 857 + Hin4I GAYNNNNNVTC 838 833 870 865 847 857 + Eam1105I GACNNNNNGTC 852 851 . . 847 852 + AcyI GRCGYC 848 850 . . 847 852 + AatII GACGTC 851 847 . . 848 851 + TaiI ACGT 851 847 . . 848 851 + SetI ASST 851 847 . . 848 851 + MaeII ACGT 848 850 . . 844 854 - FalI AAGNNNNNCTT 867 862 835 830 847 857 - Hin4I GAYNNNNNVTC 870 865 838 833 858 867 + MslI CAYNNNNRTG 862 862 . . 866 869 + CviRI* TGCA 867 867 . . 867 878 + AlfI GCANNNNNNTGC 856 854 890 888 873 876 + TaiI ACGT 876 872 . . 873 876 + SetI ASST 876 872 . . 873 876 + MaeII ACGT 873 875 . . 867 878 - AlfI GCANNNNNNTGC 890 888 856 854 889 893 + SfaNI GCATC 898 902 . . 896 899 + MaeI CTAG 896 898 . . 907 910 + TspEI AATT 906 910 . . 928 932 + TfiI GAWTC 928 931 . . 928 932 + HinfI GANTC 928 931 . . 928 937 + BsaBI GATNNNNATC 932 932 . . 930 935 + AsuII TTCGAA 931 933 . . 931 934 + TaqI TCGA 931 933 . . 933 937 + TfiI GAWTC 933 936 . . 933 937 + HinfI GANTC 933 936 . . 938 943 + TatI WGTACW 938 942 . . 938 943 + Bsp1407I TGTACA 938 942 . . 939 944 + Hpy166II GTNNAC 941 941 . . 939 942 + Csp6I GTAC 939 941 . . 939 942 + RsaI GTAC 940 940 . . 949 953 + Hpy188I TCNGA 951 950 . . 950 954 + DdeI CTNAG 950 953 . . 950 954 - BspCNI CTCAG 942 940 . . 950 954 - BseMII CTCAG 941 939 . . 957 962 + XhoII RGATCY 957 961 . . 957 962 + NlaIV GGNNCC 959 959 . . 957 962 + BamHI GGATCC 957 961 . . 957 961 + BinI* GGATC 965 966 . . 954 958 - Hin4II* CCTTC 948 947 . . 958 961 + DpnI GATC 959 959 . . 958 961 + BstKTI GATC 960 958 . . 958 961 + MboI GATC 957 961 . . 958 962 - BinI* GGATC 952 953 . . 967 970 + TspEI AATT 966 970 . . 972 976 + DdeI CTNAG 972 975 . . 972 976 + BspCNI CTCAG 985 983 . . 972 976 + BseMII CTCAG 986 984 . . 975 978 + CviJI RGCY 976 976 . . 981 985 + TfiI GAWTC 981 984 . . 981 985 + HinfI GANTC 981 984 . . 985 990 - BsiI* CACGAG 985 989 . . 991 994 + MseI TTAA 991 993 . . 996 999 + SetI ASST 999 995 . . 995 998 - MnlI CCTC 988 987 . . 1005 1015 + FalI AAGNNNNNCTT 996 991 1028 1023 1012 1022 + MwoI GCNNNNNNNGC 1018 1015 . . 1005 1015 - FalI AAGNNNNNCTT 1028 1023 996 991 1020 1023 + CviJI RGCY 1021 1021 . . 1019 1024 - TsoI TARCCA 1009 1007 . . 1024 1029 + SspI AATATT 1026 1026 . . 1031 1034 + SetI ASST 1034 1030 . . 1031 1034 + CviJI RGCY 1032 1032 . . 1031 1034 + AluI AGCT 1032 1032 . . 1039 1042 + TspEI AATT 1038 1042 . . 1051 1056 + Cfr10I RCCGGY 1051 1055 . . 1051 1056 + BetI* WCCGGW 1051 1055 . . 1051 1056 + AgeI ACCGGT 1051 1055 . . 1052 1055 + HpaII CCGG 1052 1054 . . 1073 1077 - MboII GAAGA 1065 1064 . . 1092 1097 + Esp3I CGTCTC 1098 1102 . . 1093 1097 + BsmAI GTCTC 1098 1102 . . 1109 1112 + MseI TTAA 1109 1111 . . 1113 1118 + HaeII RGCGCY 1117 1113 . . 1114 1117 + Hin6I GCGC 1114 1116 . . 1114 1117 + GlaI GCGC 1115 1115 . . 1114 1117 + HhaI GCGC 1116 1114 . . 1143 1148 + SspI AATATT 1145 1145 . . 1153 1157 - BsmAI GTCTC 1147 1151 . . 1162 1165 + TspEI AATT 1161 1165 . . 1166 1170 - MboII GAAGA 1158 1157 . . 1179 1183 + MboII GAAGA 1191 1190 . . 1184 1188 + BsrI ACTGG 1189 1187 . . 1184 1189 + BfiI ACTGGG 1194 1193 . . 1202 1212 + FalI AAGNNNNNCTT 1193 1188 1225 1220 1204 1209 + NlaIV GGNNCC 1206 1206 . . 1204 1209 + HgiCI* GGYRCC 1204 1208 . . 1202 1212 - FalI AAGNNNNNCTT 1225 1220 1193 1188 1214 1218 + Hpy188I TCNGA 1216 1215 . . 1217 1227 + Hin4I GAYNNNNNVTC 1208 1203 1240 1235 1217 1227 - Hin4I GAYNNNNNVTC 1240 1235 1208 1203 1232 1236 + Hin4II* CCTTC 1242 1241 . . 1227 1232 - BtgZI GCGATG 1212 1216 . . 1237 1240 + MnlI CCTC 1247 1246 . . 1238 1243 + SmlI CTYRAG 1238 1242 . . 1241 1251 + FalI AAGNNNNNCTT 1232 1227 1264 1259 1238 1243 - Bce83I* CTTGAG 1223 1221 . . 1247 1252 + Ksp632I* CTCTTC 1253 1256 . . 1241 1251 - FalI AAGNNNNNCTT 1264 1259 1232 1227 1248 1252 - MboII GAAGA 1240 1239 . . 1264 1269 + ApoI RAATTY 1264 1268 . . 1265 1268 + TspEI AATT 1264 1268 . . 1271 1275 + DdeI CTNAG 1271 1274 . . 1271 1275 + BspCNI CTCAG 1284 1282 . . 1271 1275 + BseMII CTCAG 1285 1283 . . 1274 1277 + SetI ASST 1277 1273 . . 1276 1281 + Hpy166II GTNNAC 1278 1278 . . 1276 1281 + HindII GTYRAC 1278 1278 . . 1283 1288 - Eco57MI CTGRAG 1268 1266 . . 1283 1288 - GsuI CTGGAG 1268 1266 . . 1300 1304 - SfaNI GCATC 1290 1294 . . 1303 1306 - AciI CCGC 1303 1305 . . 1311 1316 + SspI AATATT 1313 1313 . . 1319 1324 + BclI TGATCA 1319 1323 . . 1320 1323 + DpnI GATC 1321 1321 . . 1320 1323 + BstKTI GATC 1322 1320 . . 1320 1323 + MboI GATC 1319 1323 . . 1329 1333 + BslFI GGGAC 1343 1347 . . 1360 1364 - TspDTI ATGAA 1350 1348 . . 1370 1374 + MboII GAAGA 1382 1381 . . 1373 1383 + BplI GAGNNNNNCTC 1364 1359 1396 1391 1375 1379 + PleI GAGTC 1383 1384 . . 1375 1379 + MlyI GAGTC 1384 1384 . . 1375 1379 + HinfI GANTC 1375 1378 . . 1370 1375 - Ksp632I* CTCTTC 1365 1368 . . 1377 1381 + BsmAI GTCTC 1382 1386 . . 1381 1385 + DdeI CTNAG 1381 1384 . . 1381 1385 + BspCNI CTCAG 1394 1392 . . 1381 1385 + BseMII CTCAG 1395 1393 . . 1382 1386 + Hpy188I TCNGA 1384 1383 . . 1373 1383 - BplI GAGNNNNNCTC 1396 1391 1364 1359 1395 1399 + TfiI GAWTC 1395 1398 . . 1395 1399 + HinfI GANTC 1395 1398 . . 1404 1407 + Csp6I GTAC 1404 1406 . . 1404 1407 + RsaI GTAC 1405 1405 . . 1406 1409 + SetI ASST 1409 1405 . . 1415 1419 + Tsp4CI* ACNGT 1417 1416 . . 1416 1422 + TspRI CASTGNN 1422 1413 . . 1423 1426 + SetI ASST 1426 1422 . . 1430 1435 + Hpy178III* TCNNGA 1431 1433 . . 1454 1457 + TaiI ACGT 1457 1453 . . 1454 1457 + SetI ASST 1457 1453 . . 1454 1457 + MaeII ACGT 1454 1456 . . 1460 1463 + TspEI AATT 1459 1463 . . 1484 1487 + CviJI RGCY 1485 1485 . . 1498 1501 + MseI TTAA 1498 1500 . . 1512 1517 + SmlI CTYRAG 1512 1516 . . 1512 1517 + AflII CTTAAG 1512 1516 . . 1513 1516 + MseI TTAA 1513 1515 . . 1516 1521 + XhoII RGATCY 1516 1520 . . 1517 1520 + DpnI GATC 1518 1518 . . 1517 1520 + BstKTI GATC 1519 1517 . . 1517 1520 + MboI GATC 1516 1520 . . 1517 1521 - BinI* GGATC 1511 1512 . . 1531 1535 - BceAI ACGGC 1516 1518 . . 1545 1550 + StyI CCWWGG 1545 1549 . . 1545 1550 + SecI* CCNNGG 1545 1549 . . 1545 1555 + BsiYI* CCNNNNNNNGG 1551 1548 . . 1548 1551 + SetI ASST 1551 1547 . . 1554 1557 + CviJI RGCY 1555 1555 . . 1556 1561 + SmlI CTYRAG 1556 1560 . . 1561 1566 + BsrDI GCAATG 1568 1566 . . 1556 1561 - Bce83I* CTTGAG 1541 1539 . . 1565 1570 + BclI TGATCA 1565 1569 . . 1566 1569 + DpnI GATC 1567 1567 . . 1566 1569 + BstKTI GATC 1568 1566 . . 1566 1569 + MboI GATC 1565 1569 . . 1585 1588 + MaeI CTAG 1585 1587 . . 1587 1590 + SetI ASST 1590 1586 . . 1587 1590 + CviJI RGCY 1588 1588 . . 1587 1590 + AluI AGCT 1588 1588 . . 1601 1605 + BsmAI GTCTC 1606 1610 . . 1607 1612 - MmeI TCCRAC 1588 1586 . . 1648 1653 + SspI AATATT 1650 1650 . . 1658 1662 - TspDTI ATGAA 1648 1646 . . 1665 1669 + HphI GGTGA 1677 1676 . . 1667 1678 + BdaI TGANNNNNNTCA 1656 1654 1690 1688 1668 1673 + ApoI RAATTY 1668 1672 . . 1669 1672 + TspEI AATT 1668 1672 . . 1673 1678 + BclI TGATCA 1673 1677 . . 1674 1677 + DpnI GATC 1675 1675 . . 1674 1677 + BstKTI GATC 1676 1674 . . 1674 1677 + MboI GATC 1673 1677 . . 1667 1678 - BdaI TGANNNNNNTCA 1690 1688 1656 1654 1679 1684 + EcoT22I ATGCAT 1683 1679 . . 1680 1683 + CviRI* TGCA 1681 1681 . . 1705 1708 + TspEI AATT 1704 1708 . . 1721 1726 + EcoP15I CAGCAG 1751 1753 . . 1723 1728 + BtsI GCAGTG 1730 1728 . . 1724 1730 + TspRI CASTGNN 1730 1721 . . 1723 1734 - BcgI CGANNNNNNTGC 1746 1744 1712 1710 1736 1742 + TspRI CASTGNN 1742 1733 . . 1740 1744 - BccI CCATC 1734 1735 . . 1744 1747 - AciI CCGC 1744 1746 . . 1767 1770 + FnuDII* CGCG 1768 1768 . . 1768 1778 + MwoI GCNNNNNNNGC 1774 1771 . . 1768 1771 - AciI CCGC 1768 1770 . . 1794 1799 + Hpy166II GTNNAC 1796 1796 . . 1804 1808 + Tsp45I GTSAC 1803 1808 . . 1804 1808 + MaeIII GTNAC 1803 1808 . . 1807 1810 + SetI ASST 1810 1806 . . 1805 1809 - HphI GGTGA 1797 1796 . . 1810 1815 + TatI WGTACW 1810 1814 . . 1810 1815 + Bsp1407I TGTACA 1810 1814 . . 1811 1816 + Hpy166II GTNNAC 1813 1813 . . 1811 1814 + Csp6I GTAC 1811 1813 . . 1811 1814 + RsaI GTAC 1812 1812 . . 1814 1820 + TspRI CASTGNN 1820 1811 . . 1815 1819 + BsrI ACTGG 1820 1818 . . 1847 1851 + MaeIII GTNAC 1846 1851 . . 1844 1848 - TspGWI ACGGA 1834 1832 . . 1858 1862 + TspDTI ATGAA 1873 1871 . . 1867 1877 + Hin4I GAYNNNNNVTC 1858 1853 1890 1885 1867 1877 + Eam1105I GACNNNNNGTC 1872 1871 . . 1868 1872 + Tsp4CI* ACNGT 1870 1869 . . 1867 1877 - Hin4I GAYNNNNNVTC 1890 1885 1858 1853 1879 1883 - TspDTI ATGAA 1869 1867 . . 1886 1890 + DdeI CTNAG 1886 1889 . . 1890 1894 + TfiI GAWTC 1890 1893 . . 1890 1894 + HinfI GANTC 1890 1893 . . 1904 1907 + TaqI TCGA 1904 1906 . . 1906 1910 - BccI CCATC 1900 1901 . . 1924 1928 + TfiI GAWTC 1924 1927 . . 1924 1928 + HinfI GANTC 1924 1927 . . 1927 1931 + Hpy188I TCNGA 1929 1928 . . 1932 1936 + Tsp4CI* ACNGT 1934 1933 . . 1940 1943 + TspEI AATT 1939 1943 . . 1950 1953 + SetI ASST 1953 1949 . . 1950 1953 + CviJI RGCY 1951 1951 . . 1950 1953 + AluI AGCT 1951 1951 . . 1967 1971 + TspDTI ATGAA 1982 1980 . . 1975 1978 - MnlI CCTC 1968 1967 . . 1978 1982 + MboII GAAGA 1990 1989 . . 1994 1998 + Tsp45I GTSAC 1993 1998 . . 1994 1998 + MaeIII GTNAC 1993 1998 . . 1991 1995 - TspGWI ACGGA 1981 1979 . . 2015 2018 + SetI ASST 2018 2014 . . 2019 2024 + BsmI GAATGC 2025 2023 . . 2022 2025 + CviRI* TGCA 2023 2023 . . 2042 2046 + HinfI GANTC 2042 2045 . . 2042 2046 - PleI GAGTC 2036 2037 . . 2042 2046 - MlyI GAGTC 2036 2036 . . 2059 2064 + SpeI ACTAGT 2059 2063 . . 2060 2063 + MaeI CTAG 2060 2062 . . 2056 2060 - BsmAI GTCTC 2050 2054 . . 2064 2068 - TspDTI ATGAA 2054 2052 . . 2069 2072 + MnlI CCTC 2079 2078 . . 2066 2070 - FokI GGATG 2052 2056 . . 2066 2070 - BseGI GGATG 2065 2063 . . 2075 2078 + MseI TTAA 2075 2077 . . 2082 2087 + ApoI RAATTY 2082 2086 . . 2083 2086 + TspEI AATT 2082 2086 . . 2087 2090 + MnlI CCTC 2097 2096 . . 2093 2097 + Hin4II* CCTTC 2103 2102 . . 2096 2099 + TaqI TCGA 2096 2098 . . 2102 2105 + MnlI CCTC 2112 2111 . . 2098 2102 - SfaNI GCATC 2088 2092 . . 2102 2107 + SecI* CCNNGG 2102 2106 . . 2104 2108 + Hpy188I TCNGA 2106 2105 . . 2110 2114 + MaeIII GTNAC 2109 2114 . . 2113 2117 + TspGWI ACGGA 2128 2126 . . 2118 2128 + MwoI GCNNNNNNNGC 2124 2121 . . 2118 2128 + BstAPI GCANNNNNTGC 2124 2121 . . 2121 2124 + SetI ASST 2124 2120 . . 2126 2129 + CviRI* TGCA 2127 2127 . . 2134 2137 + SetI ASST 2137 2133 . . 2135 2138 + MnlI CCTC 2145 2144 . . 2138 2143 + NspBII* CMGCKG 2140 2140 . . 2138 2141 + AciI CCGC 2138 2140 . . 2145 2148 + FatI CATG 2144 2148 . . 2145 2148 + NlaIII CATG 2148 2144 . . 2145 2148 + CviAII CATG 2145 2147 . . 2150 2153 + Csp6I GTAC 2150 2152 . . 2150 2153 + RsaI GTAC 2151 2151 . . 2159 2163 + BlsI GCNGC 2161 2160 . . 2159 2163 + BisI GCNGC 2160 2161 . . 2159 2163 + BbvI GCAGC 2171 2175 . . 2159 2163 + TseI GCWGC 2159 2162 . . 2161 2164 + SetI ASST 2164 2160 . . 2161 2164 + CviJI RGCY 2162 2162 . . 2161 2164 + AluI AGCT 2162 2162 . . 2169 2173 + BsmAI GTCTC 2174 2178 . . 2175 2179 + Tsp4CI* ACNGT 2177 2176 . . 2178 2181 + Csp6I GTAC 2178 2180 . . 2178 2181 + RsaI GTAC 2179 2179 . . 2184 2187 + TaiI ACGT 2187 2183 . . 2184 2187 + SetI ASST 2187 2183 . . 2184 2187 + MaeII ACGT 2184 2186 . . 2188 2198 + MwoI GCNNNNNNNGC 2194 2191 . . 2188 2192 - HgaI GACGC 2177 2182 . . 2204 2207 + Csp6I GTAC 2204 2206 . . 2204 2207 + RsaI GTAC 2205 2205 . . 2212 2215 + MaeI CTAG 2212 2214 . . 2214 2219 + HaeII RGCGCY 2218 2214 . . 2215 2218 + Hin6I GCGC 2215 2217 . . 2215 2218 + GlaI GCGC 2216 2216 . . 2215 2218 + HhaI GCGC 2217 2215 . . 2233 2236 + SetI ASST 2236 2232 . . 2234 2237 + MnlI CCTC 2244 2243 . . 2235 2240 - Eco57MI CTGRAG 2220 2218 . . 2235 2240 - GsuI CTGGAG 2220 2218 . . 2237 2241 - BsrI ACTGG 2237 2235 . . 2242 2245 + MseI TTAA 2242 2244 . . 2245 2249 + TspDTI ATGAA 2260 2258 . . 2249 2252 + CviJI RGCY 2250 2250 . . 2254 2258 + TfiI GAWTC 2254 2257 . . 2254 2258 + HinfI GANTC 2254 2257 . . 2261 2265 + BsmAI GTCTC 2266 2270 . . 2267 2270 + SetI ASST 2270 2266 . . 2268 2272 + BssKI CCNGG 2267 2272 . . 2268 2272 + BseBI CCWGG 2269 2270 . . 2268 2273 + SecI* CCNNGG 2268 2272 . . 2268 2272 + ScrFI CCNGG 2269 2270 . . 2268 2272 + EcoRII CCWGG 2267 2272 . . 2274 2279 + EcoRI GAATTC 2274 2278 . . 2274 2279 + ApoI RAATTY 2274 2278 . . 2275 2278 + TspEI AATT 2274 2278 . . 2300 2303 + MseI TTAA 2300 2302 . . 2309 2313 + BceAI ACGGC 2325 2327 . . 2312 2316 + BlsI GCNGC 2314 2313 . . 2312 2316 + BisI GCNGC 2313 2314 . . 2312 2316 + BbvI GCAGC 2324 2328 . . 2312 2316 + TseI GCWGC 2312 2315 . . 2326 2331 + Cac8I GCNNGC 2328 2328 . . 2327 2331 + FauI CCCGC 2335 2337 . . 2328 2331 + AciI CCGC 2328 2330 . . #--------------------------------------- # # # AatII: # [GACGTC] # 851 1501 # # AciI: # [CCGC] # 16 24 24 56 57 165 # 190 370 431 441 578 # # AcyI: # [GRCGYC] # 97 751 1504 # # AflII: # [CTTAAG] # 840 1512 # # AflIII: # [ACRYGT] # 37 96 311 1908 # # AgeI: # [ACCGGT] # 1051 1301 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 856 1462 # # AluI: # [AGCT] # 159 190 194 211 261 363 # 418 556 # # AlwNI: # [CAGNNNCTG] # 622 1730 # # ApoI: # [RAATTY] # 78 178 192 263 297 404 # 414 526 # # AsuI*: # [GGNCC] # 198 236 315 1603 # # AsuII: # [TTCGAA] # 452 479 1421 # # AvaII: # [GGWCC] # 749 1603 # # BamHI: # [GGATCC] # 957 1395 # # BbvI: # [GCAGC] # 28 65 96 153 183 364 # 1463 # # BbvII*: # [GAAGAC] # 529 1823 # # BccI: # [CCATC] # 32 166 452 655 1047 # # Bce83I*: # [CTTGAG] # 156 318 531 536 811 # # BceAI: # [ACGGC] # 27 173 809 1343 # # BcgI: # [CGANNNNNNTGC] # 34 606 1712 # # BclI: # [TGATCA] # 108 246 679 1319 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 662 1656 # # BetI*: # [WCCGGW] # 1051 1301 # # BfiI: # [ACTGGG] # 1158 1194 # # BinI*: # [GGATC] # 13 546 841 952 # # BisI: # [GCNGC] # 3 15 39 54 66 96 # 153 208 283 1435 # # BlsI: # [GCNGC] # 3 15 38 55 66 96 # 153 208 283 1435 # # BmgT120I: # [GGNCC] # 198 236 316 1602 # # BplI: # [GAGNNNNNCTC] # 0 0 32 956 1364 # # BsaAI: # [YACGTR] # 356 445 1551 # # BsaBI: # [GATNNNNATC] # 932 1420 # # BseBI: # [CCWGG] # 47 83 216 286 1720 # # BseGI: # [GGATG] # 287 382 1683 # # BseMII: # [CTCAG] # 21 27 45 106 110 227 # 248 299 312 957 # # BseYI: # [CCCAGC] # 343 2009 # # BsgI: # [GTGCAG] # 72 2280 # # BsiI*: # [CACGAG] # 985 1367 # # BsiYI*: # [CCNNNNNNNGG] # 23 801 1528 # # BslFI: # [GGGAC] # 1009 1343 # # BsmAI: # [GTCTC] # 49 86 92 120 124 224 # 235 273 444 705 # # BsmI: # [GAATGC] # 327 2025 # # Bsp1407I: # [TGTACA] # 542 872 938 # # BspCNI: # [CTCAG] # 21 27 43 106 110 225 # 249 299 314 958 # # BsrDI: # [GCAATG] # 21 63 78 147 407 784 # 852 # # BsrI: # [ACTGG] # 115 417 631 1189 # # BssKI: # [CCNGG] # 47 85 98 118 284 1720 # # BstAPI: # [GCANNNNNTGC] # 228 2124 # # BstKTI: # [GATC] # 5 49 108 197 231 352 # 362 372 676 # # BtgZI: # [GCGATG] # 1140 1212 # # BtrI: # [CACGTC] # 134 2218 # # BtsI: # [GCAGTG] # 63 620 622 1047 # # Cac8I: # [GCNNGC] # 24 491 1837 # # CauII*: # [CCSGG] # 404 1948 # # Cfr10I: # [RCCGGY] # 1051 1301 # # Csp6I: # [GTAC] # 4 13 26 28 46 137 # 138 148 218 339 383 407 # 465 # # CspCI: # [CAANNNNNGTGG] # 35 812 1505 # # CviAII: # [CATG] # 24 207 360 393 1368 # # CviJI: # [RGCY] # 5 5 11 12 30 33 # 42 45 56 60 67 70 # 88 102 129 138 176 211 # 256 363 453 # # CviRI*: # [TGCA] # 11 50 53 87 92 104 # 157 225 342 417 814 # # DdeI: # [CTNAG] # 21 22 27 80 106 110 # 124 257 299 335 466 505 # # DpnI: # [GATC] # 4 49 108 197 231 352 # 362 372 677 # # DsaI*: # [CCRYGG] # 752 1600 # # Eam1105I: # [GACNNNNNGTC] # 280 480 572 1020 # # Eco57MI: # [CTGRAG] # 132 952 1268 # # EcoNI: # [CCTNNNNNAGG] # 21 2331 # # EcoP15I: # [CAGCAG] # 595 601 1156 # # EcoRI: # [GAATTC] # 78 475 1799 # # EcoRII: # [CCWGG] # 47 85 216 284 1720 # # EcoT22I: # [ATGCAT] # 669 1683 # # Esp3I: # [CGTCTC] # 1098 1254 # # EspI*: # [GCTNAGC] # 257 2095 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 0 0 7 32 # 32 32 32 32 129 165 # 311 492 1088 # # FatI: # [CATG] # 24 208 359 393 1368 # # FauI: # [CCCGC] # 17 2335 # # FnuDII*: # [CGCG] # 584 727 1041 # # FokI: # [GGATG] # 300 369 1683 # # GlaI: # [GCGC] # 136 1101 1115 # # GsaI: # [CCCAGC] # 347 2005 # # GsuI: # [CTGGAG] # 132 952 1268 # # HaeII: # [RGCGCY] # 134 1101 1117 # # HaeIII: # [GGCC] # 5 230 256 317 1544 # # HgaI: # [GACGC] # 11 86 115 140 175 184 # 205 1436 # # HgiCI*: # [GGYRCC] # 26 1148 1178 # # HhaI: # [GCGC] # 135 1101 1116 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 32 32 # 32 32 32 48 266 338 # 460 462 618 # # Hin4II*: # [CCTTC] # 27 219 249 294 702 861 # # Hin6I: # [GCGC] # 137 1101 1114 # # HindII: # [GTYRAC] # 1074 1278 # # HinfI: # [GANTC] # 5 20 34 48 98 118 # 212 379 394 495 549 # # HpaII: # [CCGG] # 94 310 648 1300 # # HphI: # [GGTGA] # 120 384 555 1293 # # Hpy166II: # [GTNNAC] # 17 151 210 215 337 365 # 518 539 # # Hpy178III*: # [TCNNGA] # 86 424 921 921 # # Hpy188I: # [TCNGA] # 75 168 177 246 265 389 # 487 545 # # Hpy99I: # [CGWCG] # 3 228 2121 # # Ksp632I*: # [CTCTTC] # 112 987 1253 # # MaeI: # [CTAG] # 140 152 220 475 676 689 # # MaeII: # [ACGT] # 25 29 37 48 67 109 # 168 202 356 581 730 # # MaeIII: # [GTNAC] # 43 96 116 147 243 243 # 271 1193 # # MboI: # [GATC] # 2 49 108 197 231 352 # 362 372 679 # # MboII: # [GAAGA] # 33 49 93 142 362 531 # 534 608 # # MlyI: # [GAGTC] # 316 652 1384 # # MmeI: # [TCCRAC] # 665 764 923 # # MnlI: # [CCTC] # 15 18 33 81 99 108 # 111 259 391 516 721 # # MseI: # [TTAA] # 15 34 52 58 79 118 # 120 167 389 562 758 # # MslI: # [CAYNNNNRTG] # 174 318 370 1490 # # MwoI: # [GCNNNNNNNGC] # 9 51 70 158 333 350 # 625 756 # # NcoI: # [CCATGG] # 752 1600 # # NlaIII: # [CATG] # 24 204 363 393 1368 # # NlaIV: # [GGNNCC] # 28 247 249 682 1146 # # NspBII*: # [CMGCKG] # 56 212 433 1651 # # OliI: # [CACNNNNGTG] # 370 1982 # # PfoI: # [TCCNGGA] # 284 2068 # # PleI: # [GAGTC] # 316 653 1383 # # PsrI: # [GAACNNNNNNTAC] # 32 51 2269 # # RsaI: # [GTAC] # 4 13 26 28 47 137 # 138 147 218 339 383 407 # 465 # # SauI*: # [CCTNAGG] # 588 1764 # # ScrFI: # [CCNGG] # 47 83 98 118 286 1720 # # SecI*: # [CCNNGG] # 84 166 311 441 557 793 # # SetI: # [ASST] # 9 11 12 13 16 17 # 18 19 23 25 27 29 # 31 34 35 37 39 49 # 65 67 73 82 92 94 # 106 109 111 122 123 126 # 132 143 220 243 # # SfaNI: # [GCATC] # 169 264 392 729 798 # # SmlI: # [CTYRAG] # 44 192 274 510 536 796 # # SnaBI: # [TACGTA] # 801 1551 # # SpeI: # [ACTAGT] # 293 2059 # # SspI: # [AATATT] # 119 168 337 702 1026 # # StyI: # [CCWWGG] # 311 441 793 807 # # TaiI: # [ACGT] # 25 29 40 48 67 109 # 165 202 356 581 730 # # TaqI: # [TCGA] # 102 192 256 377 452 973 # # TaqII: # [CACCCA] # 44 685 1623 # # TatI: # [WGTACW] # 359 542 579 872 # # TauI: # [GCSGC] # 15 165 547 1625 # # TfiI: # [GAWTC] # 5 34 48 98 330 379 # 414 495 549 # # TseI: # [GCWGC] # 40 53 96 153 208 364 # 1438 # # TsoI: # [TARCCA] # 1009 1343 # # Tsp45I: # [GTSAC] # 190 243 359 1560 # # Tsp4CI*: # [ACNGT] # 48 50 64 151 175 243 # 279 453 889 # # TspDTI: # [ATGAA] # 4 72 92 109 117 206 # 221 298 585 648 # # TspEI: # [AATT] # 7 27 36 60 72 78 # 78 103 123 143 144 168 # 192 195 209 219 235 263 # # TspGWI: # [ACGGA] # 147 147 200 224 287 1347 # # TspRI: # [CASTGNN] # 12 63 78 94 252 281 # 308 532 732 # # TstI: # [CACNNNNNNTCC] # 32 622 1698 # # XcmI: # [CCANNNNNNNNNTGG] # 426 1926 # # XhoII: # [RGATCY] # 559 836 957 # # ZraI: # [GACGTC] # 849 1503 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2352 # Reported_sequences: 1 # Reported_hitcount: 606 #---------------------------------------