Restriction Fragments for CDC24/YAL041W



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The currently selected gene/sequence is :

CDC24/YAL041W on chromosome I from coordinates 62840 to 65404.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.11430.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 9 + DpnI GATC 7 7 . . 6 9 + BstKTI GATC 8 6 . . 6 9 + MboI GATC 5 9 . . 23 26 + MnlI CCTC 33 32 . . 23 28 + SecI* CCNNGG 23 27 . . 24 29 + BmeT110I CYCGRG 25 27 . . 24 29 + AvaI CYCGRG 24 28 . . 27 32 + SduI GDGCHC 31 27 . . 27 32 + BseSI GKGCMC 31 27 . . 40 44 + Hpy188I TCNGA 42 41 . . 60 70 + FalI AAGNNNNNCTT 51 46 83 78 63 66 + CviRI* TGCA 64 64 . . 60 70 - FalI AAGNNNNNCTT 83 78 51 46 71 75 + BccI CCATC 79 80 . . 58 71 - AjuI GAANNNNNNNTTGG 83 78 51 46 78 87 + MslI CAYNNNNRTG 82 82 . . 84 87 + FatI CATG 83 87 . . 84 87 + NlaIII CATG 87 83 . . 84 87 + CviAII CATG 84 86 . . 86 89 + CviRI* TGCA 87 87 . . 95 100 + Hpy178III* TCNNGA 96 98 . . 95 100 + BspHI TCATGA 95 99 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 97 101 + TspDTI ATGAA 112 110 . . 104 107 + CviJI RGCY 105 105 . . 109 113 + Tsp45I GTSAC 108 113 . . 109 113 + MaeIII GTNAC 108 113 . . 112 116 + TspGWI ACGGA 127 125 . . 121 125 + HinfI GANTC 121 124 . . 125 131 + TspRI CASTGNN 131 122 . . 121 125 - PleI GAGTC 115 116 . . 121 125 - MlyI GAGTC 115 115 . . 126 130 + Tsp4CI* ACNGT 128 127 . . 120 132 - AloI GAACNNNNNNTCC 144 139 112 107 139 144 + MstI* TGCGCA 141 141 . . 140 143 + Hin6I GCGC 140 142 . . 140 143 + GlaI GCGC 141 141 . . 140 143 + HhaI GCGC 142 140 . . 149 154 + Hpy178III* TCNNGA 150 152 . . 149 154 + BetI* WCCGGW 149 153 . . 149 154 + BspMII* TCCGGA 149 153 . . 150 153 + HpaII CCGG 150 152 . . 147 151 - FokI GGATG 133 137 . . 147 151 - BseGI GGATG 146 144 . . 159 163 + BsrI ACTGG 164 162 . . 160 165 + Eco57MI CTGRAG 181 179 . . 160 165 + GsuI CTGGAG 181 179 . . 164 167 + SetI ASST 167 163 . . 163 166 - MnlI CCTC 156 155 . . 168 172 + MaeIII GTNAC 167 172 . . 171 174 + SetI ASST 174 170 . . 172 175 + MnlI CCTC 182 181 . . 183 186 + SetI ASST 186 182 . . 193 198 + MfeI CAATTG 193 197 . . 194 197 + TspEI AATT 193 197 . . 198 201 + CviJI RGCY 199 199 . . 198 201 + HaeIII GGCC 199 199 . . 208 211 + TaqI TCGA 208 210 . . 215 218 + SetI ASST 218 214 . . 214 217 - MnlI CCTC 207 206 . . 255 259 + BlsI GCNGC 257 256 . . 255 259 + BisI GCNGC 256 257 . . 255 259 + BbvI GCAGC 267 271 . . 255 259 + TseI GCWGC 255 258 . . 258 262 + SfaNI GCATC 267 271 . . 261 266 + Hpy178III* TCNNGA 262 264 . . 262 270 + AlwNI CAGNNNCTG 267 264 . . 271 276 + SmlI CTYRAG 271 275 . . 271 276 - Bce83I* CTTGAG 256 254 . . 283 288 + HaeII RGCGCY 287 283 . . 284 287 + Hin6I GCGC 284 286 . . 284 287 + GlaI GCGC 285 285 . . 284 287 + HhaI GCGC 286 284 . . 291 295 + BssKI CCNGG 290 295 . . 291 294 + HpaII CCGG 291 293 . . 291 295 + ScrFI CCNGG 292 293 . . 291 295 + CauII* CCSGG 292 293 . . 293 297 + BslFI GGGAC 307 311 . . 296 300 + Tsp4CI* ACNGT 298 297 . . 309 314 + NlaIV GGNNCC 311 311 . . 309 314 + HgiCI* GGYRCC 309 313 . . 319 322 + MseI TTAA 319 321 . . 322 325 + SetI ASST 325 321 . . 326 329 + MaeI CTAG 326 328 . . 328 331 + SetI ASST 331 327 . . 328 331 + CviJI RGCY 329 329 . . 328 331 + AluI AGCT 329 329 . . 342 345 + SetI ASST 345 341 . . 342 345 + CviJI RGCY 343 343 . . 342 345 + AluI AGCT 343 343 . . 350 354 + BsmAI GTCTC 355 359 . . 347 351 - BsrI ACTGG 347 345 . . 354 363 + MslI CAYNNNNRTG 358 358 . . 354 357 + FatI CATG 353 357 . . 354 357 + NlaIII CATG 357 353 . . 354 357 + CviAII CATG 354 356 . . 358 361 + TaqI TCGA 358 360 . . 360 364 - BccI CCATC 354 355 . . 366 369 + SetI ASST 369 365 . . 364 368 - Hin4II* CCTTC 358 357 . . 381 386 + EcoRI GAATTC 381 385 . . 381 386 + ApoI RAATTY 381 385 . . 382 385 + TspEI AATT 381 385 . . 384 389 + AsuII TTCGAA 385 387 . . 385 388 + TaqI TCGA 385 387 . . 387 391 + TfiI GAWTC 387 390 . . 387 391 + HinfI GANTC 387 390 . . 392 395 + MnlI CCTC 402 401 . . 392 397 + SecI* CCNNGG 392 396 . . 395 400 + CfrI YGGCCR 395 399 . . 396 399 + CviJI RGCY 397 397 . . 396 399 + HaeIII GGCC 397 397 . . 398 412 + XcmI CCANNNNNNNNNTGG 405 404 . . 393 398 - NmeAIII GCCGAG 373 371 . . 402 412 + BsiYI* CCNNNNNNNGG 408 405 . . 412 415 - MnlI CCTC 405 404 . . 449 460 + BstXI CCANNNNNNTGG 456 452 . . 450 459 + MslI CAYNNNNRTG 454 454 . . 455 460 + DsaI* CCRYGG 455 459 . . 455 460 + StyI CCWWGG 455 459 . . 455 460 + NcoI CCATGG 455 459 . . 455 460 + SecI* CCNNGG 455 459 . . 456 459 + FatI CATG 455 459 . . 456 459 + NlaIII CATG 459 455 . . 456 459 + CviAII CATG 456 458 . . 472 476 + Tsp45I GTSAC 471 476 . . 472 476 + MaeIII GTNAC 471 476 . . 487 492 + PvuII CAGCTG 489 489 . . 487 492 + NspBII* CMGCKG 489 489 . . 488 491 + SetI ASST 491 487 . . 488 491 + CviJI RGCY 489 489 . . 488 491 + AluI AGCT 489 489 . . 501 504 + SetI ASST 504 500 . . 505 508 + Hin6I GCGC 505 507 . . 505 508 + GlaI GCGC 506 506 . . 505 508 + HhaI GCGC 507 505 . . 510 513 + MnlI CCTC 520 519 . . 515 520 + Hpy166II GTNNAC 517 517 . . 515 520 + BssNAI GTATAC 517 517 . . 515 520 + AccI GTMKAC 516 518 . . 536 539 + CviJI RGCY 537 537 . . 537 541 + TauI GCSGC 540 537 . . 537 541 + BlsI GCNGC 539 538 . . 537 541 + BisI GCNGC 538 539 . . 538 541 + AciI CCGC 538 540 . . 542 545 + TspEI AATT 541 545 . . 543 550 + SwaI ATTTAAAT 546 546 . . 544 549 + AhaIII* TTTAAA 546 546 . . 545 548 + MseI TTAA 545 547 . . 548 551 + TspEI AATT 547 551 . . 552 557 + Cfr10I RCCGGY 552 556 . . 552 557 + BetI* WCCGGW 552 556 . . 552 557 + AgeI ACCGGT 552 556 . . 553 556 + HpaII CCGG 553 555 . . 562 566 + SfaNI GCATC 571 575 . . 565 569 + Hpy188I TCNGA 567 566 . . 613 616 + CviJI RGCY 614 614 . . 614 618 + BlsI GCNGC 616 615 . . 614 618 + BisI GCNGC 615 616 . . 614 618 + TseI GCWGC 614 617 . . 616 619 + CviRI* TGCA 617 617 . . 614 618 - BbvI GCAGC 601 605 . . 619 629 + FalI AAGNNNNNCTT 610 605 642 637 619 629 - FalI AAGNNNNNCTT 642 637 610 605 630 633 + CviRI* TGCA 631 631 . . 635 638 + MseI TTAA 635 637 . . 643 648 + BseRI GAGGAG 658 656 . . 643 646 - MnlI CCTC 636 635 . . 647 650 + SetI ASST 650 646 . . 647 650 + CviJI RGCY 648 648 . . 647 650 + AluI AGCT 648 648 . . 661 666 + MmeI TCCRAC 686 684 . . 661 665 + Hpy188I TCNGA 663 662 . . 663 667 + Hpy99I CGWCG 667 662 . . 663 674 + BcgI CGANNNNNNTGC 652 650 686 684 665 668 + TaiI ACGT 668 664 . . 665 668 + SetI ASST 668 664 . . 665 668 + MaeII ACGT 665 667 . . 686 691 + GsaI CCCAGC 690 686 . . 686 691 + BseYI CCCAGC 686 690 . . 688 693 + PvuII CAGCTG 690 690 . . 688 693 + NspBII* CMGCKG 690 690 . . 689 692 + SetI ASST 692 688 . . 689 692 + CviJI RGCY 690 690 . . 689 692 + AluI AGCT 690 690 . . 703 706 + MaeI CTAG 703 705 . . 724 728 + TspDTI ATGAA 739 737 . . 726 731 + EcoRI GAATTC 726 730 . . 726 731 + ApoI RAATTY 726 730 . . 727 730 + TspEI AATT 726 730 . . 733 736 + CviJI RGCY 734 734 . . 749 752 + MnlI CCTC 759 758 . . 752 756 + DdeI CTNAG 752 755 . . 773 778 + Hpy178III* TCNNGA 774 776 . . 773 778 + BspHI TCATGA 773 777 . . 774 777 + FatI CATG 773 777 . . 774 777 + NlaIII CATG 777 773 . . 774 777 + CviAII CATG 774 776 . . 775 779 + TspDTI ATGAA 790 788 . . 803 806 + CviJI RGCY 804 804 . . 805 808 + MnlI CCTC 815 814 . . 805 811 + Bpu10I CCTNAGC 806 809 . . 805 811 + BbvCI CCTCAGC 806 809 . . 806 810 + DdeI CTNAG 806 809 . . 806 810 + BspCNI CTCAG 819 817 . . 806 810 + BseMII CTCAG 820 818 . . 808 813 + EcoP15I CAGCAG 838 840 . . 816 821 + AsuII TTCGAA 817 819 . . 817 820 + TaqI TCGA 817 819 . . 814 818 - MboII GAAGA 806 805 . . 813 818 - BbvII* GAAGAC 806 810 . . 819 823 + MboII GAAGA 831 830 . . 839 842 + MseI TTAA 839 841 . . 843 846 + TspEI AATT 842 846 . . 853 858 + EcoRI GAATTC 853 857 . . 853 858 + ApoI RAATTY 853 857 . . 854 857 + TspEI AATT 853 857 . . 861 864 + CviRI* TGCA 862 862 . . 865 869 + TspGWI ACGGA 880 878 . . 880 885 + Hpy166II GTNNAC 882 882 . . 882 887 + Hpy178III* TCNNGA 883 885 . . 893 898 + ApoI RAATTY 893 897 . . 894 897 + TspEI AATT 893 897 . . 913 918 + EcoP15I CAGCAG 943 945 . . 940 943 + TaiI ACGT 943 939 . . 940 943 + SetI ASST 943 939 . . 940 943 + MaeII ACGT 940 942 . . 943 947 + Hpy188I TCNGA 945 944 . . 944 949 + Eco57MI CTGRAG 965 963 . . 944 949 + Eco57I CTGAAG 965 963 . . 946 950 + MboII GAAGA 958 957 . . 946 951 - Ksp632I* CTCTTC 941 944 . . 953 958 + TatI WGTACW 953 957 . . 953 958 + Bsp1407I TGTACA 953 957 . . 954 957 + Csp6I GTAC 954 956 . . 954 957 + RsaI GTAC 955 955 . . 956 961 + BspLU11I* ACATGT 956 960 . . 956 961 + NspI RCATGY 960 956 . . 956 961 + AflIII ACRYGT 956 960 . . 957 960 + FatI CATG 956 960 . . 957 960 + NlaIII CATG 960 956 . . 957 960 + CviAII CATG 957 959 . . 954 966 - PsrI GAACNNNNNNTAC 978 973 946 941 966 976 + BsiYI* CCNNNNNNNGG 972 969 . . 967 977 + BsiYI* CCNNNNNNNGG 973 970 . . 970 973 + TspEI AATT 969 973 . . 976 980 + HphI GGTGA 988 987 . . 974 979 - TaqII CACCCA 964 962 . . 979 983 - SfaNI GCATC 969 973 . . 983 988 + SfeI* CTRYAG 983 987 . . 998 1002 + MboII GAAGA 1010 1009 . . 1013 1018 + StyI CCWWGG 1013 1017 . . 1013 1018 + SecI* CCNNGG 1013 1017 . . 1038 1041 + SetI ASST 1041 1037 . . 1039 1043 + Hin4II* CCTTC 1049 1048 . . 1052 1056 + TfiI GAWTC 1052 1055 . . 1052 1056 + HinfI GANTC 1052 1055 . . 1058 1063 + SduI GDGCHC 1062 1058 . . 1058 1063 + HgiJII* GRGCYC 1062 1058 . . 1059 1062 + CviJI RGCY 1060 1060 . . 1068 1071 + FatI CATG 1067 1071 . . 1068 1071 + NlaIII CATG 1071 1067 . . 1068 1071 + CviAII CATG 1068 1070 . . 1069 1074 + EcoT22I ATGCAT 1073 1069 . . 1066 1070 - TspDTI ATGAA 1056 1054 . . 1070 1073 + CviRI* TGCA 1071 1071 . . 1071 1076 - BsmI GAATGC 1071 1069 . . 1088 1091 + MseI TTAA 1088 1090 . . 1100 1103 + CviJI RGCY 1101 1101 . . 1102 1107 + StyI CCWWGG 1102 1106 . . 1102 1107 + SecI* CCNNGG 1102 1106 . . 1099 1109 - Hin4I GAYNNNNNVTC 1122 1117 1090 1085 1113 1118 + BalI TGGCCA 1115 1115 . . 1113 1118 + CfrI YGGCCR 1113 1117 . . 1114 1117 + CviJI RGCY 1115 1115 . . 1114 1117 + HaeIII GGCC 1115 1115 . . 1122 1125 + CviRI* TGCA 1123 1123 . . 1123 1127 + BlsI GCNGC 1125 1124 . . 1123 1127 + BisI GCNGC 1124 1125 . . 1123 1127 + BbvI GCAGC 1135 1139 . . 1123 1127 + TseI GCWGC 1123 1126 . . 1125 1128 + CviJI RGCY 1126 1126 . . 1127 1131 + BccI CCATC 1135 1136 . . 1130 1133 + TaqI TCGA 1130 1132 . . 1132 1137 + ApoI RAATTY 1132 1136 . . 1133 1136 + TspEI AATT 1132 1136 . . 1140 1145 + Ksp632I* CTCTTC 1146 1149 . . 1141 1145 - MboII GAAGA 1133 1132 . . 1151 1154 + CviRI* TGCA 1152 1152 . . 1161 1164 - MnlI CCTC 1154 1153 . . 1169 1173 + TspDTI ATGAA 1184 1182 . . 1171 1175 + TfiI GAWTC 1171 1174 . . 1171 1175 + HinfI GANTC 1171 1174 . . 1176 1180 + BlsI GCNGC 1178 1177 . . 1176 1180 + BisI GCNGC 1177 1178 . . 1176 1180 + BbvI GCAGC 1188 1192 . . 1176 1180 + TseI GCWGC 1176 1179 . . 1177 1182 + NspBII* CMGCKG 1179 1179 . . 1179 1182 - AciI CCGC 1179 1181 . . 1183 1187 - TspDTI ATGAA 1173 1171 . . 1188 1191 + TspEI AATT 1187 1191 . . 1190 1193 + MseI TTAA 1190 1192 . . 1200 1204 + BsrI ACTGG 1205 1203 . . 1205 1208 + TspEI AATT 1204 1208 . . 1208 1211 + CviRI* TGCA 1209 1209 . . 1214 1218 + Hin4II* CCTTC 1224 1223 . . 1221 1226 + PsiI TTATAA 1223 1223 . . 1229 1239 + BsiYI* CCNNNNNNNGG 1235 1232 . . 1232 1235 + CviRI* TGCA 1233 1233 . . 1238 1241 + CviJI RGCY 1239 1239 . . 1247 1252 + EcoRV GATATC 1249 1249 . . 1265 1275 + FalI AAGNNNNNCTT 1256 1251 1288 1283 1268 1271 + TspEI AATT 1267 1271 . . 1272 1277 + Cac8I GCNNGC 1274 1274 . . 1265 1275 - FalI AAGNNNNNCTT 1288 1283 1256 1251 1279 1283 + TfiI GAWTC 1279 1282 . . 1279 1283 + HinfI GANTC 1279 1282 . . 1282 1285 + TaqI TCGA 1282 1284 . . 1286 1290 + Tsp45I GTSAC 1285 1290 . . 1286 1290 + MaeIII GTNAC 1285 1290 . . 1314 1317 + SetI ASST 1317 1313 . . 1314 1317 + CviJI RGCY 1315 1315 . . 1314 1317 + AluI AGCT 1315 1315 . . 1315 1319 + BlsI GCNGC 1317 1316 . . 1315 1319 + BisI GCNGC 1316 1317 . . 1315 1319 + TseI GCWGC 1315 1318 . . 1315 1319 - BbvI GCAGC 1302 1306 . . 1336 1341 + SspI AATATT 1338 1338 . . 1367 1371 + MboII GAAGA 1379 1378 . . 1389 1393 + HphI GGTGA 1401 1400 . . 1392 1396 + MboII GAAGA 1404 1403 . . 1414 1419 + Hpy166II GTNNAC 1416 1416 . . 1414 1419 + HindII GTYRAC 1416 1416 . . 1418 1422 + BsrI ACTGG 1423 1421 . . 1422 1426 - Hin4II* CCTTC 1416 1415 . . 1433 1438 + ApoI RAATTY 1433 1437 . . 1434 1437 + TspEI AATT 1433 1437 . . 1447 1451 + HphI GGTGA 1459 1458 . . 1463 1466 + TaqI TCGA 1463 1465 . . 1474 1478 - TspDTI ATGAA 1464 1462 . . 1492 1495 + SetI ASST 1495 1491 . . 1492 1495 + CviJI RGCY 1493 1493 . . 1492 1495 + AluI AGCT 1493 1493 . . 1496 1499 + MnlI CCTC 1506 1505 . . 1496 1501 + SecI* CCNNGG 1496 1500 . . 1498 1502 + Hpy188I TCNGA 1500 1499 . . 1494 1499 - BseRI GAGGAG 1485 1483 . . 1500 1505 + NlaIV GGNNCC 1502 1502 . . 1503 1506 + SetI ASST 1506 1502 . . 1513 1518 + ApoI RAATTY 1513 1517 . . 1514 1517 + TspEI AATT 1513 1517 . . 1520 1523 + SetI ASST 1523 1519 . . 1519 1522 - MnlI CCTC 1512 1511 . . 1545 1549 - FokI GGATG 1531 1535 . . 1545 1549 - BseGI GGATG 1544 1542 . . 1555 1559 + Hpy188I TCNGA 1557 1556 . . 1559 1562 + SetI ASST 1562 1558 . . 1558 1561 - MnlI CCTC 1551 1550 . . 1564 1568 + Tsp45I GTSAC 1563 1568 . . 1564 1568 + MaeIII GTNAC 1563 1568 . . 1568 1572 + DdeI CTNAG 1568 1571 . . 1578 1581 + CviRI* TGCA 1579 1579 . . 1579 1583 + SfaNI GCATC 1588 1592 . . 1594 1599 + SmlI CTYRAG 1594 1598 . . 1594 1599 + AflII CTTAAG 1594 1598 . . 1595 1598 + MseI TTAA 1595 1597 . . 1599 1603 + MboII GAAGA 1611 1610 . . 1607 1610 + MnlI CCTC 1617 1616 . . 1612 1615 + SetI ASST 1615 1611 . . 1613 1616 + MnlI CCTC 1623 1622 . . 1613 1619 + Bpu10I CCTNAGC 1614 1617 . . 1613 1619 + BbvCI CCTCAGC 1614 1617 . . 1614 1618 + DdeI CTNAG 1614 1617 . . 1614 1618 + BspCNI CTCAG 1627 1625 . . 1614 1618 + BseMII CTCAG 1628 1626 . . 1618 1622 + SfaNI GCATC 1627 1631 . . 1628 1631 + AciI CCGC 1628 1630 . . 1631 1634 + MnlI CCTC 1641 1640 . . 1627 1632 - EciI GGCGGA 1617 1615 . . 1633 1636 + TaqI TCGA 1633 1635 . . 1642 1646 + TspGWI ACGGA 1657 1655 . . 1655 1659 + BlsI GCNGC 1657 1656 . . 1655 1659 + BisI GCNGC 1656 1657 . . 1655 1659 + BbvI GCAGC 1667 1671 . . 1655 1659 + TseI GCWGC 1655 1658 . . 1657 1660 + CviJI RGCY 1658 1658 . . 1660 1663 + MnlI CCTC 1670 1669 . . 1662 1666 - HphI GGTGA 1654 1653 . . 1667 1671 + Tsp4CI* ACNGT 1669 1668 . . 1670 1674 + MaeIII GTNAC 1669 1674 . . 1680 1683 - MnlI CCTC 1673 1672 . . 1720 1724 - MboII GAAGA 1712 1711 . . 1726 1730 - MboII GAAGA 1718 1717 . . 1730 1735 + EcoP15I CAGCAG 1760 1762 . . 1725 1730 - BbvII* GAAGAC 1718 1722 . . 1727 1732 - Eco57MI CTGRAG 1712 1710 . . 1732 1736 + BlsI GCNGC 1734 1733 . . 1732 1736 + BisI GCNGC 1733 1734 . . 1732 1736 + BbvI GCAGC 1744 1748 . . 1732 1736 + TseI GCWGC 1732 1735 . . 1727 1732 - Eco57I CTGAAG 1712 1710 . . 1733 1738 + NspBII* CMGCKG 1735 1735 . . 1735 1739 + TauI GCSGC 1738 1735 . . 1735 1739 + BlsI GCNGC 1737 1736 . . 1735 1739 + BisI GCNGC 1736 1737 . . 1735 1738 - AciI CCGC 1735 1737 . . 1754 1757 + Csp6I GTAC 1754 1756 . . 1754 1757 + RsaI GTAC 1755 1755 . . 1751 1755 - BsrI ACTGG 1751 1749 . . 1766 1770 + Tsp45I GTSAC 1765 1770 . . 1766 1770 + MaeIII GTNAC 1765 1770 . . 1774 1777 + TspEI AATT 1773 1777 . . 1777 1782 + MmeI TCCRAC 1802 1800 . . 1783 1786 + TspEI AATT 1782 1786 . . 1785 1789 - TspDTI ATGAA 1775 1773 . . 1796 1799 + MnlI CCTC 1806 1805 . . 1793 1797 - FokI GGATG 1779 1783 . . 1793 1797 - BseGI GGATG 1792 1790 . . 1808 1811 + SetI ASST 1811 1807 . . 1808 1811 + CviJI RGCY 1809 1809 . . 1808 1811 + AluI AGCT 1809 1809 . . 1814 1817 + AciI CCGC 1814 1816 . . 1824 1827 + SetI ASST 1827 1823 . . 1825 1831 + Bpu10I CCTNAGC 1826 1829 . . 1826 1830 + DdeI CTNAG 1826 1829 . . 1829 1832 + SetI ASST 1832 1828 . . 1829 1832 + CviJI RGCY 1830 1830 . . 1829 1832 + AluI AGCT 1830 1830 . . 1833 1838 + XhoII RGATCY 1833 1837 . . 1833 1837 + BinI* GGATC 1841 1842 . . 1834 1837 + DpnI GATC 1835 1835 . . 1834 1837 + BstKTI GATC 1836 1834 . . 1834 1837 + MboI GATC 1833 1837 . . 1837 1841 + DdeI CTNAG 1837 1840 . . 1842 1845 + SetI ASST 1845 1841 . . 1841 1844 - MnlI CCTC 1834 1833 . . 1845 1848 + TaqI TCGA 1845 1847 . . 1848 1851 + TspEI AATT 1847 1851 . . 1854 1864 + Hin4I GAYNNNNNVTC 1845 1840 1877 1872 1858 1862 + TspDTI ATGAA 1873 1871 . . 1860 1864 + TfiI GAWTC 1860 1863 . . 1860 1864 + HinfI GANTC 1860 1863 . . 1863 1867 + Hpy188I TCNGA 1865 1864 . . 1854 1864 - Hin4I GAYNNNNNVTC 1877 1872 1845 1840 1865 1876 + BdaI TGANNNNNNTCA 1854 1852 1888 1886 1866 1870 + TfiI GAWTC 1866 1869 . . 1866 1870 + HinfI GANTC 1866 1869 . . 1865 1876 - BdaI TGANNNNNNTCA 1888 1886 1854 1852 1879 1882 + AciI CCGC 1879 1881 . . 1889 1894 + Cfr10I RCCGGY 1889 1893 . . 1889 1894 + BetI* WCCGGW 1889 1893 . . 1889 1894 + AgeI ACCGGT 1889 1893 . . 1890 1893 + HpaII CCGG 1890 1892 . . 1897 1900 + MseI TTAA 1897 1899 . . 1907 1910 + FatI CATG 1906 1910 . . 1907 1910 + NlaIII CATG 1910 1906 . . 1907 1910 + CviAII CATG 1907 1909 . . 1912 1916 + TfiI GAWTC 1912 1915 . . 1912 1916 + HinfI GANTC 1912 1915 . . 1929 1932 + SetI ASST 1932 1928 . . 1933 1936 + TspEI AATT 1932 1936 . . 1945 1950 + ApoI RAATTY 1945 1949 . . 1946 1949 + TspEI AATT 1945 1949 . . 1957 1960 - MnlI CCTC 1950 1949 . . 1969 1979 + Hin4I GAYNNNNNVTC 1960 1955 1992 1987 1972 1975 + TspEI AATT 1971 1975 . . 1969 1979 - Hin4I GAYNNNNNVTC 1992 1987 1960 1955 1985 1990 + Hpy166II GTNNAC 1987 1987 . . 1992 1996 + Tsp4CI* ACNGT 1994 1993 . . 1998 2002 + TfiI GAWTC 1998 2001 . . 1998 2002 + HinfI GANTC 1998 2001 . . 1998 2008 + Hin4I GAYNNNNNVTC 1989 1984 2021 2016 2001 2006 + Hpy178III* TCNNGA 2002 2004 . . 2001 2006 + BspHI TCATGA 2001 2005 . . 2002 2005 + FatI CATG 2001 2005 . . 2002 2005 + NlaIII CATG 2005 2001 . . 2002 2005 + CviAII CATG 2002 2004 . . 2000 2004 - TspDTI ATGAA 1990 1988 . . 2005 2008 + DpnI GATC 2006 2006 . . 2005 2008 + BstKTI GATC 2007 2005 . . 2005 2008 + MboI GATC 2004 2008 . . 2007 2011 + Hpy188I TCNGA 2009 2008 . . 1998 2008 - Hin4I GAYNNNNNVTC 2021 2016 1989 1984 2024 2027 + MseI TTAA 2024 2026 . . 2040 2045 + Ksp632I* CTCTTC 2046 2049 . . 2041 2045 - MboII GAAGA 2033 2032 . . 2050 2053 + TaqI TCGA 2050 2052 . . 2051 2055 + Hpy99I CGWCG 2055 2050 . . 2058 2062 - TspDTI ATGAA 2048 2046 . . 2062 2065 + TaqI TCGA 2062 2064 . . 2067 2070 + CviJI RGCY 2068 2068 . . 2077 2081 - MboII GAAGA 2069 2068 . . 2088 2092 + Tsp45I GTSAC 2087 2092 . . 2088 2092 + MaeIII GTNAC 2087 2092 . . 2090 2095 + TaqII CACCCA 2106 2104 . . 2089 2093 - HphI GGTGA 2081 2080 . . 2097 2106 + MslI CAYNNNNRTG 2101 2101 . . 2104 2108 + TspDTI ATGAA 2119 2117 . . 2115 2119 + TfiI GAWTC 2115 2118 . . 2115 2119 + HinfI GANTC 2115 2118 . . 2128 2131 + CviJI RGCY 2129 2129 . . 2129 2133 + TauI GCSGC 2132 2129 . . 2129 2133 + BlsI GCNGC 2131 2130 . . 2129 2133 + BisI GCNGC 2130 2131 . . 2130 2133 + AciI CCGC 2130 2132 . . 2151 2154 + CviJI RGCY 2152 2152 . . 2160 2165 + SmlI CTYRAG 2160 2164 . . 2160 2165 - Bce83I* CTTGAG 2145 2143 . . 2170 2175 + NdeI CATATG 2171 2173 . . 2173 2177 + TspDTI ATGAA 2188 2186 . . 2185 2189 + Hpy188I TCNGA 2187 2186 . . 2187 2191 + FokI GGATG 2200 2204 . . 2187 2191 + BseGI GGATG 2193 2191 . . 2202 2206 + TspGWI ACGGA 2217 2215 . . 2207 2211 + BmgT120I GGNCC 2208 2209 . . 2207 2211 + AvaII GGWCC 2207 2210 . . 2207 2211 + AsuI* GGNCC 2207 2210 . . 2205 2208 - MnlI CCTC 2198 2197 . . 2214 2218 - TspDTI ATGAA 2204 2202 . . 2224 2229 + AsuII TTCGAA 2225 2227 . . 2225 2228 + TaqI TCGA 2225 2227 . . 2235 2238 + TspEI AATT 2234 2238 . . 2237 2240 + MseI TTAA 2237 2239 . . 2248 2252 + Hpy188I TCNGA 2250 2249 . . 2253 2258 + ApoI RAATTY 2253 2257 . . 2254 2257 + TspEI AATT 2253 2257 . . 2258 2263 + Hpy178III* TCNNGA 2259 2261 . . 2271 2276 + Hpy178III* TCNNGA 2272 2274 . . 2275 2279 + TfiI GAWTC 2275 2278 . . 2275 2279 + HinfI GANTC 2275 2278 . . 2278 2282 - MboII GAAGA 2270 2269 . . 2287 2292 + Ksp632I* CTCTTC 2293 2296 . . 2291 2296 + Hpy178III* TCNNGA 2292 2294 . . 2288 2292 - MboII GAAGA 2280 2279 . . 2289 2294 - Eco57MI CTGRAG 2274 2272 . . 2289 2294 - Eco57I CTGAAG 2274 2272 . . 2295 2300 + EcoRV GATATC 2297 2297 . . 2323 2326 + SetI ASST 2326 2322 . . 2324 2327 + MnlI CCTC 2334 2333 . . 2327 2330 + MaeI CTAG 2327 2329 . . 2336 2341 + XhoII RGATCY 2336 2340 . . 2336 2341 + BglII AGATCT 2336 2340 . . 2337 2340 + DpnI GATC 2338 2338 . . 2337 2340 + BstKTI GATC 2339 2337 . . 2337 2340 + MboI GATC 2336 2340 . . 2339 2343 - MboII GAAGA 2331 2330 . . 2367 2372 + ApoI RAATTY 2367 2371 . . 2368 2371 + TspEI AATT 2367 2371 . . 2391 2394 + DpnI GATC 2392 2392 . . 2391 2394 + BstKTI GATC 2393 2391 . . 2391 2394 + MboI GATC 2390 2394 . . 2395 2398 + TspEI AATT 2394 2398 . . 2402 2407 + ApoI RAATTY 2402 2406 . . 2403 2406 + TspEI AATT 2402 2406 . . 2406 2411 + AsuII TTCGAA 2407 2409 . . 2407 2410 + TaqI TCGA 2407 2409 . . 2431 2435 - HphI GGTGA 2423 2422 . . 2438 2442 - HphI GGTGA 2430 2429 . . 2445 2455 + Hin4I GAYNNNNNVTC 2436 2431 2468 2463 2445 2448 + DpnI GATC 2446 2446 . . 2445 2448 + BstKTI GATC 2447 2445 . . 2445 2448 + MboI GATC 2444 2448 . . 2454 2459 + Hpy178III* TCNNGA 2455 2457 . . 2445 2455 - Hin4I GAYNNNNNVTC 2468 2463 2436 2431 2461 2465 + MboII GAAGA 2473 2472 . . 2464 2468 - BccI CCATC 2458 2459 . . 2484 2487 + SetI ASST 2487 2483 . . 2489 2494 + BtgZI GCGATG 2504 2508 . . 2492 2496 + TspDTI ATGAA 2507 2505 . . 2494 2498 + MboII GAAGA 2506 2505 . . 2523 2528 + EciI GGCGGA 2539 2537 . . 2524 2527 - AciI CCGC 2524 2526 . . 2539 2544 + ApoI RAATTY 2539 2543 . . 2540 2543 + TspEI AATT 2539 2543 . . 2543 2548 + Hpy178III* TCNNGA 2544 2546 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 516 2049 # # AciI: # [CCGC] # 41 65 79 107 251 394 # 449 538 641 # # AflII: # [CTTAAG] # 971 1594 # # AflIII: # [ACRYGT] # 956 1609 # # AgeI: # [ACCGGT] # 552 676 1337 # # AhaIII*: # [TTTAAA] # 546 2019 # # AjuI: # [GAANNNNNNNTTGG] # 32 51 2482 # # AloI: # [GAACNNNNNNTCC] # 32 112 2421 # # AluI: # [AGCT] # 14 21 42 146 159 178 # 316 329 625 735 # # AlwNI: # [CAGNNNCTG] # 267 2298 # # ApoI: # [RAATTY] # 26 35 40 80 114 127 # 137 239 301 308 345 381 # 432 # # AsuI*: # [GGNCC] # 358 2207 # # AsuII: # [TTCGAA] # 158 182 385 432 1408 # # AvaI: # [CYCGRG] # 24 2541 # # AvaII: # [GGWCC] # 358 2207 # # BalI: # [TGGCCA] # 1115 1450 # # BbvCI: # [CCTCAGC] # 806 808 951 # # BbvI: # [GCAGC] # 53 77 114 267 334 365 # 534 821 # # BbvII*: # [GAAGAC] # 806 847 912 # # BccI: # [CCATC] # 79 107 275 781 1323 # # Bce83I*: # [CTTGAG] # 256 420 1889 # # BcgI: # [CGANNNNNNTGC] # 34 652 1879 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 677 1854 # # BetI*: # [WCCGGW] # 149 403 676 1337 # # BglII: # [AGATCT] # 229 2336 # # BinI*: # [GGATC] # 724 1841 # # BisI: # [GCNGC] # 3 53 77 77 139 256 # 282 340 394 435 509 # # BlsI: # [GCNGC] # 3 53 77 77 139 257 # 282 340 394 434 509 # # BmeT110I: # [CYCGRG] # 25 2540 # # BmgT120I: # [GGNCC] # 357 2208 # # Bpu10I: # [CCTNAGC] # 212 739 806 808 # # BseGI: # [GGATG] # 146 248 372 401 1398 # # BseMII: # [CTCAG] # 808 820 937 # # BseRI: # [GAGGAG] # 658 827 1080 # # BseSI: # [GKGCMC] # 31 2534 # # BseYI: # [CCCAGC] # 686 1879 # # BsiYI*: # [CCNNNNNNNGG] # 1 262 408 564 1330 # # BslFI: # [GGGAC] # 307 2258 # # BsmAI: # [GTCTC] # 355 2210 # # BsmI: # [GAATGC] # 1071 1494 # # Bsp1407I: # [TGTACA] # 953 1612 # # BspCNI: # [CTCAG] # 808 819 938 # # BspHI: # [TCATGA] # 95 564 678 1228 # # BspLU11I*: # [ACATGT] # 956 1609 # # BspMII*: # [TCCGGA] # 149 2416 # # BsrI: # [ACTGG] # 164 183 218 328 814 858 # # BssKI: # [CCNGG] # 290 2275 # # BssNAI: # [GTATAC] # 517 2048 # # BstKTI: # [GATC] # 8 54 54 118 171 332 # 1828 # # BstXI: # [CCANNNNNNTGG] # 456 2109 # # BtgZI: # [GCGATG] # 61 2504 # # Cac8I: # [GCNNGC] # 1274 1291 # # CauII*: # [CCSGG] # 292 2273 # # Cfr10I: # [RCCGGY] # 552 676 1337 # # CfrI: # [YGGCCR] # 395 718 1452 # # Csp6I: # [GTAC] # 800 811 954 # # CviAII: # [CATG] # 12 84 95 102 111 183 # 258 318 563 839 # # CviJI: # [RGCY] # 11 14 14 21 23 34 # 41 42 44 48 54 61 # 70 76 77 92 94 105 # 113 130 151 165 178 238 # 256 413 # # CviRI*: # [TGCA] # 14 23 24 29 52 57 # 64 209 231 346 530 986 # # DdeI: # [CTNAG] # 11 46 54 212 728 752 # 762 # # DpnI: # [GATC] # 7 54 54 119 171 332 # 1828 # # DsaI*: # [CCRYGG] # 455 2110 # # EciI: # [GGCGGA] # 26 922 1617 # # Eco57I: # [CTGAAG] # 291 562 747 965 # # Eco57MI: # [CTGRAG] # 181 291 562 747 784 # # EcoP15I: # [CAGCAG] # 105 805 817 838 # # EcoRI: # [GAATTC] # 127 345 381 1712 # # EcoRV: # [GATATC] # 268 1048 1249 # # EcoT22I: # [ATGCAT] # 1073 1492 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 51 527 614 # 1277 # # FatI: # [CATG] # 12 83 95 102 111 183 # 258 318 564 839 # # FokI: # [GGATG] # 133 248 365 421 1398 # # GlaI: # [GCGC] # 141 144 221 2059 # # GsaI: # [CCCAGC] # 690 1875 # # GsuI: # [CTGGAG] # 181 2384 # # HaeII: # [RGCGCY] # 287 2278 # # HaeIII: # [GGCC] # 198 199 718 1450 # # HgiCI*: # [GGYRCC] # 309 2256 # # HgiJII*: # [GRGCYC] # 1062 1503 # # HhaI: # [GCGC] # 142 144 221 2058 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 3 29 29 32 # 32 32 83 97 415 723 # 1090 # # Hin4II*: # [CCTTC] # 175 192 358 691 1149 # # Hin6I: # [GCGC] # 140 144 221 2060 # # HindII: # [GTYRAC] # 1149 1416 # # HinfI: # [GANTC] # 6 46 86 108 117 119 # 121 160 266 290 581 665 # # HpaII: # [CCGG] # 141 150 262 675 1337 # # HphI: # [GGTGA] # 7 58 135 195 342 413 # 427 988 # # Hpy166II: # [GTNNAC] # 365 517 534 571 578 # # Hpy178III*: # [TCNNGA] # 13 20 21 54 89 96 # 109 112 163 257 512 1119 # # Hpy188I: # [TCNGA] # 42 57 63 96 144 178 # 282 308 315 525 555 # # Hpy99I: # [CGWCG] # 510 667 1388 # # Ksp632I*: # [CTCTTC] # 205 247 272 900 941 # # MaeI: # [CTAG] # 238 326 377 1624 # # MaeII: # [ACGT] # 275 665 1625 # # MaeIII: # [GTNAC] # 59 96 106 108 278 304 # 322 478 814 # # MboI: # [GATC] # 5 54 54 121 171 332 # 1828 # # MboII: # [GAAGA] # 6 10 25 25 33 36 # 51 52 59 101 123 127 # 142 201 207 246 315 806 # # MfeI: # [CAATTG] # 193 2372 # # MlyI: # [GAGTC] # 115 2450 # # MmeI: # [TCCRAC] # 686 763 1116 # # MnlI: # [CCTC] # 3 3 6 6 18 25 # 26 28 29 33 39 56 # 66 115 116 116 123 123 # 133 136 195 231 248 339 # 352 # # MseI: # [TTAA] # 90 102 127 204 213 226 # 249 302 319 328 405 # # MslI: # [CAYNNNNRTG] # 82 96 276 464 1647 # # MstI*: # [TGCGCA] # 141 2424 # # NcoI: # [CCATGG] # 455 2110 # # NdeI: # [CATATG] # 394 2171 # # NlaIII: # [CATG] # 12 87 95 102 111 183 # 258 318 560 839 # # NlaIV: # [GGNNCC] # 311 1063 1191 # # NmeAIII: # [GCCGAG] # 373 2192 # # NspBII*: # [CMGCKG] # 201 489 489 556 830 # # NspI: # [RCATGY] # 960 1605 # # PleI: # [GAGTC] # 115 2450 # # PsiI: # [TTATAA] # 1223 1342 # # PsrI: # [GAACNNNNNNTAC] # 32 946 1587 # # PvuII: # [CAGCTG] # 201 489 1875 # # RsaI: # [GTAC] # 800 810 955 # # ScrFI: # [CCNGG] # 292 2273 # # SduI: # [GDGCHC] # 31 1031 1503 # # SecI*: # [CCNNGG] # 23 63 89 369 394 558 # 1069 # # SetI: # [ASST] # 5 6 7 11 12 13 # 13 14 16 17 18 24 # 24 32 39 53 78 87 # 98 107 122 146 161 167 # 178 196 251 276 394 # # SfaNI: # [GCATC] # 39 267 304 398 619 938 # # SfeI*: # [CTRYAG] # 983 1582 # # SmlI: # [CTYRAG] # 271 405 566 1323 # # SspI: # [AATATT] # 1227 1338 # # StyI: # [CCWWGG] # 89 455 558 1463 # # SwaI: # [ATTTAAAT] # 546 2019 # # TaiI: # [ACGT] # 275 668 1622 # # TaqI: # [TCGA] # 12 27 150 152 158 163 # 170 181 182 205 208 212 # 313 432 # # TaqII: # [CACCCA] # 459 964 1142 # # TatI: # [WGTACW] # 953 1612 # # TauI: # [GCSGC] # 394 433 540 1198 # # TfiI: # [GAWTC] # 6 46 86 108 117 119 # 160 290 387 581 665 # # TseI: # [GCWGC] # 53 77 139 255 340 359 # 509 833 # # Tsp45I: # [GTSAC] # 108 202 278 322 363 478 # 814 # # Tsp4CI*: # [ACNGT] # 128 170 325 571 1371 # # TspDTI: # [ATGAA] # 11 16 51 58 58 69 # 71 98 112 117 117 266 # 280 303 311 627 # # TspEI: # [AATT] # 6 8 9 11 13 17 # 19 26 26 27 40 55 # 63 65 76 80 85 114 # 116 137 160 163 166 179 # 188 193 260 263 # # TspGWI: # [ACGGA] # 127 348 560 753 777 # # TspRI: # [CASTGNN] # 131 2434 # # XcmI: # [CCANNNNNNNNNTGG] # 405 2160 # # XhoII: # [RGATCY] # 229 503 1833 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2565 # Reported_sequences: 1 # Reported_hitcount: 577 #---------------------------------------