Restriction Fragments for MYO4/YAL029C



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The currently selected gene/sequence is :

MYO4/YAL029C on chromosome I from coordinates 92270 to 87855.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30512.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 30 + AjuI GAANNNNNNNTTGG 9 4 41 36 20 24 + DdeI CTNAG 20 23 . . 29 34 + KpnI GGTACC 33 29 . . 29 34 + NlaIV GGNNCC 31 31 . . 29 34 + HgiCI* GGYRCC 29 33 . . 29 34 + Acc65I GGTACC 29 33 . . 30 33 + Csp6I GTAC 30 32 . . 30 33 + RsaI GTAC 31 31 . . 34 37 + MnlI CCTC 44 43 . . 49 52 + CviJI RGCY 50 50 . . 50 60 + MwoI GCNNNNNNNGC 56 53 . . 59 63 + TauI GCSGC 62 59 . . 59 63 + BlsI GCNGC 61 60 . . 59 63 + BisI GCNGC 60 61 . . 59 62 - AciI CCGC 59 61 . . 67 71 + Tsp45I GTSAC 66 71 . . 67 71 + MaeIII GTNAC 66 71 . . 68 72 - HphI GGTGA 60 59 . . 73 83 + FalI AAGNNNNNCTT 64 59 96 91 75 84 + XmnI GAANNNNTTC 79 79 . . 73 83 - FalI AAGNNNNNCTT 96 91 64 59 71 84 - AjuI GAANNNNNNNTTGG 96 91 64 59 88 92 - Hin4II* CCTTC 82 81 . . 93 98 + AclI AACGTT 94 96 . . 94 97 + TaiI ACGT 97 93 . . 94 97 + SetI ASST 97 93 . . 94 97 + MaeII ACGT 94 96 . . 102 106 + DdeI CTNAG 102 105 . . 113 116 + TspEI AATT 112 116 . . 120 124 + FokI GGATG 133 137 . . 120 124 + BseGI GGATG 126 124 . . 118 121 - MnlI CCTC 111 110 . . 121 125 - BccI CCATC 115 116 . . 130 134 + Tsp4CI* ACNGT 132 131 . . 133 138 + BciVI GTATCC 144 143 . . 124 134 - BsaXI ACNNNNNCTCC 146 143 116 113 151 154 + SetI ASST 154 150 . . 151 154 + CviJI RGCY 152 152 . . 151 154 + AluI AGCT 152 152 . . 154 159 + AsuII TTCGAA 155 157 . . 155 158 + TaqI TCGA 155 157 . . 162 173 + BdaI TGANNNNNNTCA 151 149 185 183 163 172 + BsaBI GATNNNNATC 167 167 . . 166 175 + BsaBI GATNNNNATC 170 170 . . 168 173 + BclI TGATCA 168 172 . . 169 172 + DpnI GATC 170 170 . . 169 172 + BstKTI GATC 171 169 . . 169 172 + MboI GATC 168 172 . . 162 173 - BdaI TGANNNNNNTCA 185 183 151 149 172 176 - FokI GGATG 158 162 . . 172 176 - BseGI GGATG 171 169 . . 183 186 + SetI ASST 186 182 . . 186 191 + TatI WGTACW 186 190 . . 187 190 + Csp6I GTAC 187 189 . . 187 190 + RsaI GTAC 188 188 . . 201 204 + SetI ASST 204 200 . . 211 215 + PleI GAGTC 219 220 . . 211 215 + MlyI GAGTC 220 220 . . 211 215 + HinfI GANTC 211 214 . . 213 218 + Hpy166II GTNNAC 215 215 . . 213 218 + AccI GTMKAC 214 216 . . 226 229 + MseI TTAA 226 228 . . 227 232 + TsoI TARCCA 243 241 . . 243 247 + Hpy188I TCNGA 245 244 . . 252 257 + Cfr10I RCCGGY 252 256 . . 253 256 + HpaII CCGG 253 255 . . 256 261 + BtsI GCAGTG 263 261 . . 257 263 + TspRI CASTGNN 263 254 . . 261 265 + BlsI GCNGC 263 262 . . 261 265 + BisI GCNGC 262 263 . . 261 265 + TseI GCWGC 261 264 . . 263 266 + CviRI* TGCA 264 264 . . 264 269 + NspI RCATGY 268 264 . . 264 269 + Cac8I GCNNGC 266 266 . . 264 269 + SphI GCATGC 268 264 . . 265 268 + FatI CATG 264 268 . . 265 268 + NlaIII CATG 268 264 . . 265 268 + CviAII CATG 265 267 . . 261 265 - BbvI GCAGC 248 252 . . 285 288 + FatI CATG 284 288 . . 285 288 + NlaIII CATG 288 284 . . 285 288 + CviAII CATG 285 287 . . 286 290 + TspDTI ATGAA 301 299 . . 328 332 + TauI GCSGC 331 328 . . 328 332 + BlsI GCNGC 330 329 . . 328 332 + BisI GCNGC 329 330 . . 329 332 + AciI CCGC 329 331 . . 349 354 + SalI GTCGAC 349 353 . . 349 354 + Hpy166II GTNNAC 351 351 . . 349 354 + HindII GTYRAC 351 351 . . 349 354 + AccI GTMKAC 350 352 . . 350 353 + TaqI TCGA 350 352 . . 359 364 + TatI WGTACW 359 363 . . 360 363 + Csp6I GTAC 360 362 . . 360 363 + RsaI GTAC 361 361 . . 366 371 + Hpy178III* TCNNGA 367 369 . . 366 371 + NruI TCGCGA 368 368 . . 367 370 + FnuDII* CGCG 368 368 . . 382 385 + TspEI AATT 381 385 . . 389 392 + MaeI CTAG 389 391 . . 407 410 + SetI ASST 410 406 . . 407 410 + CviJI RGCY 408 408 . . 407 410 + AluI AGCT 408 408 . . 408 418 + MwoI GCNNNNNNNGC 414 411 . . 409 414 + Eco57MI CTGRAG 430 428 . . 409 414 + GsuI CTGGAG 430 428 . . 411 416 + NlaIV GGNNCC 413 413 . . 413 416 + CviJI RGCY 414 414 . . 414 418 + TauI GCSGC 417 414 . . 414 418 + BlsI GCNGC 416 415 . . 414 418 + BisI GCNGC 415 416 . . 415 418 + AciI CCGC 415 417 . . 417 428 + AlfI GCANNNNNNTGC 406 404 440 438 419 422 + SetI ASST 422 418 . . 417 428 - AlfI GCANNNNNNTGC 440 438 406 404 433 443 + MwoI GCNNNNNNNGC 439 436 . . 436 441 + BseRI GAGGAG 451 449 . . 436 439 - MnlI CCTC 429 428 . . 439 442 - MnlI CCTC 432 431 . . 444 447 + Csp6I GTAC 444 446 . . 444 447 + RsaI GTAC 445 445 . . 448 451 + SetI ASST 451 447 . . 453 456 + FatI CATG 452 456 . . 453 456 + NlaIII CATG 456 452 . . 453 456 + CviAII CATG 453 455 . . 451 455 - TspDTI ATGAA 441 439 . . 456 460 + BmgT120I GGNCC 457 458 . . 456 460 + AvaII GGWCC 456 459 . . 456 460 + AsuI* GGNCC 456 459 . . 459 469 + BsiYI* CCNNNNNNNGG 465 462 . . 460 463 + FatI CATG 459 463 . . 460 463 + NlaIII CATG 463 459 . . 460 463 + CviAII CATG 460 462 . . 461 465 + TspDTI ATGAA 476 474 . . 468 471 + CviJI RGCY 469 469 . . 471 476 + TsoI TARCCA 487 485 . . 478 482 + Tsp4CI* ACNGT 480 479 . . 489 495 + TspRI CASTGNN 495 486 . . 493 497 + HphI GGTGA 505 504 . . 496 500 + TfiI GAWTC 496 499 . . 496 500 + HinfI GANTC 496 499 . . 499 504 + BetI* WCCGGW 499 503 . . 500 503 + HpaII CCGG 500 502 . . 514 518 + Tsp4CI* ACNGT 516 515 . . 516 521 + Esp3I CGTCTC 522 526 . . 517 521 + BsmAI GTCTC 522 526 . . 548 551 + MnlI CCTC 558 557 . . 549 553 + DdeI CTNAG 549 552 . . 549 553 + BspCNI CTCAG 562 560 . . 549 553 + BseMII CTCAG 563 561 . . 570 575 + Hpy178III* TCNNGA 571 573 . . 574 578 - Hin4II* CCTTC 568 567 . . 591 595 + BsmAI GTCTC 596 600 . . 599 602 + TaqI TCGA 599 601 . . 599 604 + Hpy178III* TCNNGA 600 602 . . 604 607 + CviJI RGCY 605 605 . . 608 613 + ApoI RAATTY 608 612 . . 609 612 + TspEI AATT 608 612 . . 615 618 + SetI ASST 618 614 . . 615 618 + CviJI RGCY 616 616 . . 615 618 + AluI AGCT 616 616 . . 624 634 + BsiYI* CCNNNNNNNGG 630 627 . . 627 630 + DpnI GATC 628 628 . . 627 630 + BstKTI GATC 629 627 . . 627 630 + MboI GATC 626 630 . . 630 633 + FatI CATG 629 633 . . 630 633 + NlaIII CATG 633 629 . . 630 633 + CviAII CATG 630 632 . . 636 639 + CviJI RGCY 637 637 . . 644 648 + MaeIII GTNAC 643 648 . . 667 677 + Hin4I GAYNNNNNVTC 658 653 690 685 670 673 + TspEI AATT 669 673 . . 674 677 + MnlI CCTC 684 683 . . 686 697 + AlfI GCANNNNNNTGC 675 673 709 707 695 698 + CviRI* TGCA 696 696 . . 686 697 - AlfI GCANNNNNNTGC 709 707 675 673 698 703 + ApoI RAATTY 698 702 . . 699 702 + TspEI AATT 698 702 . . 722 726 + BccI CCATC 730 731 . . 722 732 + BsiYI* CCNNNNNNNGG 728 725 . . 730 735 + NlaIV GGNNCC 732 732 . . 731 735 + BmgT120I GGNCC 732 733 . . 731 735 + AvaII GGWCC 731 734 . . 731 735 + AsuI* GGNCC 731 734 . . 738 741 + TspEI AATT 737 741 . . 745 748 + SetI ASST 748 744 . . 764 768 + BssKI CCNGG 763 768 . . 764 768 + BseBI CCWGG 765 766 . . 764 768 + ScrFI CCNGG 765 766 . . 764 768 + EcoRII CCWGG 763 768 . . 766 769 + SetI ASST 769 765 . . 779 782 + CviJI RGCY 780 780 . . 784 788 - BsmAI GTCTC 778 782 . . 796 799 + TspEI AATT 795 799 . . 813 823 + BsiYI* CCNNNNNNNGG 819 816 . . 813 823 + PflMI CCANNNNNTGG 819 816 . . 815 820 + ApoI RAATTY 815 819 . . 816 819 + TspEI AATT 815 819 . . 823 827 - Hin4II* CCTTC 817 816 . . 837 842 + Cfr10I RCCGGY 837 841 . . 837 842 + BetI* WCCGGW 837 841 . . 837 842 + AgeI ACCGGT 837 841 . . 838 841 + HpaII CCGG 838 840 . . 854 857 + CviRI* TGCA 855 855 . . 869 874 + StyI CCWWGG 869 873 . . 869 874 + SecI* CCNNGG 869 873 . . 888 893 + TsoI TARCCA 904 902 . . 891 896 + StyI CCWWGG 891 895 . . 891 896 + SecI* CCNNGG 891 895 . . 894 897 + SetI ASST 897 893 . . 896 901 + Hpy166II GTNNAC 898 898 . . 902 905 + CviJI RGCY 903 903 . . 912 915 + SetI ASST 915 911 . . 912 915 + CviJI RGCY 913 913 . . 912 915 + AluI AGCT 913 913 . . 923 927 + TspDTI ATGAA 938 936 . . 927 930 + SetI ASST 930 926 . . 927 930 + CviJI RGCY 928 928 . . 927 930 + AluI AGCT 928 928 . . 929 934 + SmlI CTYRAG 929 933 . . 929 934 + XhoI CTCGAG 929 933 . . 929 934 + BmeT110I CYCGRG 930 932 . . 929 934 + AvaI CYCGRG 929 933 . . 930 933 + TaqI TCGA 930 932 . . 930 935 + Hpy178III* TCNNGA 931 933 . . 944 949 + TsoI TARCCA 960 958 . . 948 954 + TspRI CASTGNN 954 945 . . 952 962 + Hin4I GAYNNNNNVTC 943 938 975 970 952 956 - SfaNI GCATC 942 946 . . 952 962 - Hin4I GAYNNNNNVTC 975 970 943 938 969 972 + SetI ASST 972 968 . . 979 984 + BsiI* CACGAG 979 983 . . 984 988 + HinfI GANTC 984 987 . . 986 990 + DdeI CTNAG 986 989 . . 986 990 + BspCNI CTCAG 999 997 . . 986 990 + BseMII CTCAG 1000 998 . . 982 986 - BsmAI GTCTC 976 980 . . 984 988 - PleI GAGTC 978 979 . . 984 988 - MlyI GAGTC 978 978 . . 989 992 + SetI ASST 992 988 . . 989 992 + CviJI RGCY 990 990 . . 989 992 + AluI AGCT 990 990 . . 995 1000 + ApoI RAATTY 995 999 . . 996 999 + TspEI AATT 995 999 . . 1004 1009 + ApoI RAATTY 1004 1008 . . 1005 1008 + TspEI AATT 1004 1008 . . 1011 1014 + CviRI* TGCA 1012 1012 . . 1012 1017 + Cac8I GCNNGC 1014 1014 . . 1015 1018 + CviJI RGCY 1016 1016 . . 1016 1021 + Cac8I GCNNGC 1018 1018 . . 1029 1032 + SetI ASST 1032 1028 . . 1037 1040 + TaqI TCGA 1037 1039 . . 1037 1042 + Hpy178III* TCNNGA 1038 1040 . . 1042 1046 + TspDTI ATGAA 1057 1055 . . 1052 1055 + MaeI CTAG 1052 1054 . . 1059 1063 + Hpy99I CGWCG 1063 1058 . . 1060 1064 + HgaI GACGC 1069 1074 . . 1063 1067 + SfaNI GCATC 1072 1076 . . 1075 1079 + Hpy188I TCNGA 1077 1076 . . 1078 1082 + MboII GAAGA 1090 1089 . . 1083 1093 + MwoI GCNNNNNNNGC 1089 1086 . . 1083 1093 + BstAPI GCANNNNNTGC 1089 1086 . . 1078 1083 - Ksp632I* CTCTTC 1073 1076 . . 1078 1084 - SapI GCTCTTC 1073 1076 . . 1088 1091 + SetI ASST 1091 1087 . . 1088 1093 + BspMI ACCTGC 1097 1101 . . 1091 1094 + CviRI* TGCA 1092 1092 . . 1099 1104 + NspI RCATGY 1103 1099 . . 1100 1103 + FatI CATG 1099 1103 . . 1100 1103 + NlaIII CATG 1103 1099 . . 1100 1103 + CviAII CATG 1100 1102 . . 1106 1109 + TspEI AATT 1105 1109 . . 1120 1123 + DpnI GATC 1121 1121 . . 1120 1123 + BstKTI GATC 1122 1120 . . 1120 1123 + MboI GATC 1119 1123 . . 1124 1128 + Hin4II* CCTTC 1134 1133 . . 1120 1124 - BinI* GGATC 1114 1115 . . 1129 1132 + TspEI AATT 1128 1132 . . 1134 1137 + CviRI* TGCA 1135 1135 . . 1148 1153 + Hpy178III* TCNNGA 1149 1151 . . 1162 1166 + Tsp45I GTSAC 1161 1166 . . 1162 1166 + MaeIII GTNAC 1161 1166 . . 1168 1171 + SetI ASST 1171 1167 . . 1171 1175 + Hpy188I TCNGA 1173 1172 . . 1179 1182 + TspEI AATT 1178 1182 . . 1183 1187 + Tsp45I GTSAC 1182 1187 . . 1183 1187 + MaeIII GTNAC 1182 1187 . . 1189 1192 + TspEI AATT 1188 1192 . . 1195 1198 + TspEI AATT 1194 1198 . . 1206 1211 + HaeII RGCGCY 1210 1206 . . 1206 1211 + Eco47III AGCGCT 1208 1208 . . 1207 1210 + Hin6I GCGC 1207 1209 . . 1207 1210 + GlaI GCGC 1208 1208 . . 1207 1217 + MwoI GCNNNNNNNGC 1213 1210 . . 1207 1210 + HhaI GCGC 1209 1207 . . 1212 1215 + TspEI AATT 1211 1215 . . 1217 1220 + MaeI CTAG 1217 1219 . . 1220 1224 + BslFI GGGAC 1234 1238 . . 1222 1226 + HinfI GANTC 1222 1225 . . 1222 1226 - PleI GAGTC 1216 1217 . . 1222 1226 - MlyI GAGTC 1216 1216 . . 1234 1239 + ApoI RAATTY 1234 1238 . . 1235 1238 + TspEI AATT 1234 1238 . . 1247 1258 - CspCI CAANNNNNGTGG 1271 1269 1236 1234 1262 1265 + CviJI RGCY 1263 1263 . . 1264 1267 + MaeI CTAG 1264 1266 . . 1261 1266 - TsoI TARCCA 1251 1249 . . 1267 1272 + Hpy166II GTNNAC 1269 1269 . . 1267 1272 + AccI GTMKAC 1268 1270 . . 1277 1280 + MseI TTAA 1277 1279 . . 1294 1297 + DpnI GATC 1295 1295 . . 1294 1297 + BstKTI GATC 1296 1294 . . 1294 1297 + MboI GATC 1293 1297 . . 1296 1301 + Hpy178III* TCNNGA 1297 1299 . . 1297 1303 + Bpu10I CCTNAGC 1298 1301 . . 1298 1302 + DdeI CTNAG 1298 1301 . . 1294 1298 - BinI* GGATC 1288 1289 . . 1299 1310 + BdaI TGANNNNNNTCA 1288 1286 1322 1320 1301 1304 + SetI ASST 1304 1300 . . 1301 1304 + CviJI RGCY 1302 1302 . . 1301 1304 + AluI AGCT 1302 1302 . . 1298 1302 - BspCNI CTCAG 1290 1288 . . 1298 1302 - BseMII CTCAG 1289 1287 . . 1305 1309 + BinI* GGATC 1313 1314 . . 1306 1309 + DpnI GATC 1307 1307 . . 1306 1309 + BstKTI GATC 1308 1306 . . 1306 1309 + MboI GATC 1305 1309 . . 1299 1310 - BdaI TGANNNNNNTCA 1322 1320 1288 1286 1315 1318 + DpnI GATC 1316 1316 . . 1315 1318 + BstKTI GATC 1317 1315 . . 1315 1318 + MboI GATC 1314 1318 . . 1318 1321 + FatI CATG 1317 1321 . . 1318 1321 + NlaIII CATG 1321 1317 . . 1318 1321 + CviAII CATG 1318 1320 . . 1345 1350 + EcoRV GATATC 1347 1347 . . 1369 1379 + BplI GAGNNNNNCTC 1360 1355 1392 1387 1369 1379 - BplI GAGNNNNNCTC 1392 1387 1360 1355 1381 1386 + AsuII TTCGAA 1382 1384 . . 1382 1385 + TaqI TCGA 1382 1384 . . 1388 1391 + TspEI AATT 1387 1391 . . 1409 1413 + TspDTI ATGAA 1424 1422 . . 1415 1418 + TspEI AATT 1414 1418 . . 1439 1442 + TaiI ACGT 1442 1438 . . 1439 1442 + SetI ASST 1442 1438 . . 1439 1442 + MaeII ACGT 1439 1441 . . 1448 1451 + TspEI AATT 1447 1451 . . 1459 1463 + MboII GAAGA 1471 1470 . . 1474 1477 - MnlI CCTC 1467 1466 . . 1479 1482 + TspEI AATT 1478 1482 . . 1476 1487 - TstI CACNNNNNNTCC 1500 1495 1468 1463 1492 1496 - TspDTI ATGAA 1482 1480 . . 1515 1518 + SetI ASST 1518 1514 . . 1519 1522 + CviRI* TGCA 1520 1520 . . 1547 1556 + XmnI GAANNNNTTC 1551 1551 . . 1565 1569 + TspDTI ATGAA 1580 1578 . . 1567 1571 + MboII GAAGA 1579 1578 . . 1577 1586 + BsaBI GATNNNNATC 1581 1581 . . 1582 1586 + BccI CCATC 1590 1591 . . 1588 1591 + CviJI RGCY 1589 1589 . . 1590 1594 + DdeI CTNAG 1590 1593 . . 1590 1594 + BspCNI CTCAG 1603 1601 . . 1590 1594 + BseMII CTCAG 1604 1602 . . 1591 1595 + Hpy188I TCNGA 1593 1592 . . 1595 1599 + TspDTI ATGAA 1610 1608 . . 1597 1601 + TfiI GAWTC 1597 1600 . . 1597 1601 + HinfI GANTC 1597 1600 . . 1601 1604 + FatI CATG 1600 1604 . . 1601 1604 + NlaIII CATG 1604 1600 . . 1601 1604 + CviAII CATG 1601 1603 . . 1604 1608 + BmgT120I GGNCC 1605 1606 . . 1604 1608 + AsuI* GGNCC 1604 1607 . . 1605 1608 + CviJI RGCY 1606 1606 . . 1605 1608 + HaeIII GGCC 1606 1606 . . 1607 1610 + MnlI CCTC 1617 1616 . . 1628 1631 + MseI TTAA 1628 1630 . . 1636 1639 + AciI CCGC 1636 1638 . . 1640 1643 + MnlI CCTC 1650 1649 . . 1646 1650 + TspDTI ATGAA 1661 1659 . . 1656 1661 + AsuII TTCGAA 1657 1659 . . 1657 1660 + TaqI TCGA 1657 1659 . . 1661 1664 + CviJI RGCY 1662 1662 . . 1663 1673 + BsiYI* CCNNNNNNNGG 1669 1666 . . 1667 1671 + TfiI GAWTC 1667 1670 . . 1667 1671 + HinfI GANTC 1667 1670 . . 1670 1674 + Hpy188I TCNGA 1672 1671 . . 1693 1696 + CviJI RGCY 1694 1694 . . 1718 1722 + TspDTI ATGAA 1733 1731 . . 1726 1730 - Hin4II* CCTTC 1720 1719 . . 1748 1752 - BsmAI GTCTC 1742 1746 . . 1761 1766 + StyI CCWWGG 1761 1765 . . 1761 1766 + SecI* CCNNGG 1761 1765 . . 1761 1766 + AvrII CCTAGG 1761 1765 . . 1762 1765 + MaeI CTAG 1762 1764 . . 1764 1767 + SetI ASST 1767 1763 . . 1768 1777 + MslI CAYNNNNRTG 1772 1772 . . 1770 1775 + Hpy178III* TCNNGA 1771 1773 . . 1773 1777 + FokI GGATG 1786 1790 . . 1773 1777 + BseGI GGATG 1779 1777 . . 1800 1803 + TspEI AATT 1799 1803 . . 1811 1816 + ApoI RAATTY 1811 1815 . . 1812 1815 + TspEI AATT 1811 1815 . . 1831 1835 + DdeI CTNAG 1831 1834 . . 1838 1842 + Tsp45I GTSAC 1837 1842 . . 1838 1842 + MaeIII GTNAC 1837 1842 . . 1843 1847 - SfaNI GCATC 1833 1837 . . 1848 1853 + Hpy178III* TCNNGA 1849 1851 . . 1850 1855 + Eco57MI CTGRAG 1871 1869 . . 1850 1855 + Eco57I CTGAAG 1871 1869 . . 1852 1856 + MboII GAAGA 1864 1863 . . 1852 1857 - Ksp632I* CTCTTC 1847 1850 . . 1852 1858 - SapI GCTCTTC 1847 1850 . . 1875 1884 + BsaBI GATNNNNATC 1879 1879 . . 1881 1884 + DpnI GATC 1882 1882 . . 1881 1884 + BstKTI GATC 1883 1881 . . 1881 1884 + MboI GATC 1880 1884 . . 1884 1889 + GsaI CCCAGC 1888 1884 . . 1884 1889 + BseYI CCCAGC 1884 1888 . . 1881 1885 - BinI* GGATC 1875 1876 . . 1894 1897 + MseI TTAA 1894 1896 . . 1909 1919 + BsiYI* CCNNNNNNNGG 1915 1912 . . 1913 1919 + PasI CCCWGGG 1914 1917 . . 1913 1918 + SecI* CCNNGG 1913 1917 . . 1914 1918 + BssKI CCNGG 1913 1918 . . 1914 1918 + BseBI CCWGG 1915 1916 . . 1914 1919 + SecI* CCNNGG 1914 1918 . . 1914 1918 + ScrFI CCNGG 1915 1916 . . 1914 1918 + EcoRII CCWGG 1913 1918 . . 1918 1923 + XhoII RGATCY 1918 1922 . . 1918 1922 + BinI* GGATC 1926 1927 . . 1919 1922 + DpnI GATC 1920 1920 . . 1919 1922 + BstKTI GATC 1921 1919 . . 1919 1922 + MboI GATC 1918 1922 . . 1939 1944 + BmeT110I CYCGRG 1940 1942 . . 1939 1944 + AvaI CYCGRG 1939 1943 . . 1946 1949 + TspEI AATT 1945 1949 . . 1949 1960 + BdaI TGANNNNNNTCA 1938 1936 1972 1970 1950 1959 + BsaBI GATNNNNATC 1954 1954 . . 1952 1957 + BalI TGGCCA 1954 1954 . . 1952 1957 + CfrI YGGCCR 1952 1956 . . 1953 1956 + CviJI RGCY 1954 1954 . . 1953 1956 + HaeIII GGCC 1954 1954 . . 1950 1954 - BccI CCATC 1944 1945 . . 1955 1959 + BccI CCATC 1963 1964 . . 1949 1960 - BdaI TGANNNNNNTCA 1972 1970 1938 1936 1962 1967 + ApoI RAATTY 1962 1966 . . 1963 1966 + TspEI AATT 1962 1966 . . 1976 1980 - TspDTI ATGAA 1966 1964 . . 1990 1993 + CviRI* TGCA 1991 1991 . . 2001 2006 + ApoI RAATTY 2001 2005 . . 2002 2005 + TspEI AATT 2001 2005 . . 2005 2009 + Hpy188I TCNGA 2007 2006 . . 2015 2018 + CviJI RGCY 2016 2016 . . 2017 2022 + DsaI* CCRYGG 2017 2021 . . 2017 2022 + StyI CCWWGG 2017 2021 . . 2017 2022 + NcoI CCATGG 2017 2021 . . 2017 2022 + SecI* CCNNGG 2017 2021 . . 2018 2021 + FatI CATG 2017 2021 . . 2018 2021 + NlaIII CATG 2021 2017 . . 2018 2021 + CviAII CATG 2018 2020 . . 2027 2030 + TaqI TCGA 2027 2029 . . 2032 2035 + TspEI AATT 2031 2035 . . 2037 2041 - BccI CCATC 2031 2032 . . 2051 2054 + TspEI AATT 2050 2054 . . 2053 2056 + MseI TTAA 2053 2055 . . 2058 2061 + SetI ASST 2061 2057 . . 2058 2061 + CviJI RGCY 2059 2059 . . 2058 2061 + AluI AGCT 2059 2059 . . 2081 2086 + Hpy178III* TCNNGA 2082 2084 . . 2090 2093 + FatI CATG 2089 2093 . . 2090 2093 + NlaIII CATG 2093 2089 . . 2090 2093 + CviAII CATG 2090 2092 . . 2088 2092 - TspDTI ATGAA 2078 2076 . . 2093 2098 + BsgI GTGCAG 2114 2112 . . 2094 2097 + CviRI* TGCA 2095 2095 . . 2084 2095 - TstI CACNNNNNNTCC 2108 2103 2076 2071 2095 2100 + Cac8I GCNNGC 2097 2097 . . 2098 2101 + CviJI RGCY 2099 2099 . . 2104 2115 + BstXI CCANNNNNNTGG 2111 2107 . . 2104 2108 + BccI CCATC 2112 2113 . . 2105 2114 + MslI CAYNNNNRTG 2109 2109 . . 2107 2112 + Hpy178III* TCNNGA 2108 2110 . . 2109 2119 + FalI AAGNNNNNCTT 2100 2095 2132 2127 2111 2115 - BccI CCATC 2105 2106 . . 2109 2119 - FalI AAGNNNNNCTT 2132 2127 2100 2095 2125 2130 + EcoRI GAATTC 2125 2129 . . 2125 2134 + XmnI GAANNNNTTC 2129 2129 . . 2125 2130 + ApoI RAATTY 2125 2129 . . 2126 2129 + TspEI AATT 2125 2129 . . 2138 2148 + Hin4I GAYNNNNNVTC 2129 2124 2161 2156 2145 2148 + MnlI CCTC 2155 2154 . . 2145 2155 + BsiYI* CCNNNNNNNGG 2151 2148 . . 2147 2152 + Hpy178III* TCNNGA 2148 2150 . . 2152 2156 + TspGWI ACGGA 2167 2165 . . 2157 2162 + SfeI* CTRYAG 2157 2161 . . 2161 2164 + SetI ASST 2164 2160 . . 2161 2164 + CviJI RGCY 2162 2162 . . 2161 2164 + AluI AGCT 2162 2162 . . 2174 2179 + EcoRI GAATTC 2174 2178 . . 2174 2179 + ApoI RAATTY 2174 2178 . . 2175 2178 + TspEI AATT 2174 2178 . . 2181 2186 + PsiI TTATAA 2183 2183 . . 2187 2192 + Hpy178III* TCNNGA 2188 2190 . . 2192 2195 + SetI ASST 2195 2191 . . 2193 2196 + MnlI CCTC 2203 2202 . . 2196 2206 + BsiYI* CCNNNNNNNGG 2202 2199 . . 2203 2206 - MnlI CCTC 2196 2195 . . 2212 2217 + Hpy166II GTNNAC 2214 2214 . . 2212 2217 + HindII GTYRAC 2214 2214 . . 2232 2237 + XbaI TCTAGA 2232 2236 . . 2232 2237 + Hpy178III* TCNNGA 2233 2235 . . 2233 2236 + MaeI CTAG 2233 2235 . . 2236 2240 - SfaNI GCATC 2226 2230 . . 2248 2252 + Hpy188I TCNGA 2250 2249 . . 2251 2255 + TfiI GAWTC 2251 2254 . . 2251 2255 + HinfI GANTC 2251 2254 . . 2268 2271 + TspEI AATT 2267 2271 . . 2282 2287 + ApoI RAATTY 2282 2286 . . 2283 2286 + TspEI AATT 2282 2286 . . 2290 2295 + AhaIII* TTTAAA 2292 2292 . . 2291 2294 + MseI TTAA 2291 2293 . . 2300 2305 + BsmI GAATGC 2306 2304 . . 2304 2309 + Cac8I GCNNGC 2306 2306 . . 2307 2310 + CviRI* TGCA 2308 2308 . . 2321 2324 + SetI ASST 2324 2320 . . 2321 2324 + CviJI RGCY 2322 2322 . . 2321 2324 + AluI AGCT 2322 2322 . . 2323 2327 + DdeI CTNAG 2323 2326 . . 2322 2328 - Bpu10I CCTNAGC 2323 2326 . . 2338 2342 + TspDTI ATGAA 2353 2351 . . 2342 2346 + TspDTI ATGAA 2357 2355 . . 2349 2354 + EcoT22I ATGCAT 2353 2349 . . 2350 2353 + CviRI* TGCA 2351 2351 . . 2351 2355 + SfaNI GCATC 2360 2364 . . 2385 2390 + SfeI* CTRYAG 2385 2389 . . 2390 2393 + CviJI RGCY 2391 2391 . . 2392 2397 - Eco57MI CTGRAG 2377 2375 . . 2392 2397 - Eco57I CTGAAG 2377 2375 . . 2402 2405 + CviRI* TGCA 2403 2403 . . 2408 2413 + DsaI* CCRYGG 2408 2412 . . 2408 2413 + StyI CCWWGG 2408 2412 . . 2408 2413 + NcoI CCATGG 2408 2412 . . 2408 2413 + SecI* CCNNGG 2408 2412 . . 2409 2412 + FatI CATG 2408 2412 . . 2409 2412 + NlaIII CATG 2412 2408 . . 2409 2412 + CviAII CATG 2409 2411 . . 2413 2417 + TfiI GAWTC 2413 2416 . . 2413 2417 + HinfI GANTC 2413 2416 . . 2434 2437 + CviJI RGCY 2435 2435 . . 2439 2442 + TspEI AATT 2438 2442 . . 2459 2462 + Csp6I GTAC 2459 2461 . . 2459 2462 + RsaI GTAC 2460 2460 . . 2468 2471 + TaqI TCGA 2468 2470 . . 2470 2473 + DpnI GATC 2471 2471 . . 2470 2473 + BstKTI GATC 2472 2470 . . 2470 2473 + MboI GATC 2469 2473 . . 2472 2477 + Hpy178III* TCNNGA 2473 2475 . . 2472 2477 + BspHI TCATGA 2472 2476 . . 2473 2476 + FatI CATG 2472 2476 . . 2473 2476 + NlaIII CATG 2476 2472 . . 2473 2476 + CviAII CATG 2473 2475 . . 2476 2481 + SacI GAGCTC 2480 2476 . . 2476 2481 + Ecl136II GAGCTC 2478 2478 . . 2476 2481 + SduI GDGCHC 2480 2476 . . 2476 2481 + HgiJII* GRGCYC 2480 2476 . . 2476 2481 + HgiAI* GWGCWC 2480 2476 . . 2477 2480 + SetI ASST 2480 2476 . . 2477 2480 + CviJI RGCY 2478 2478 . . 2477 2480 + AluI AGCT 2478 2478 . . 2479 2484 + SmlI CTYRAG 2479 2483 . . 2480 2485 + Hpy178III* TCNNGA 2481 2483 . . 2479 2484 - Bce83I* CTTGAG 2464 2462 . . 2490 2493 + SetI ASST 2493 2489 . . 2490 2493 + CviJI RGCY 2491 2491 . . 2490 2493 + AluI AGCT 2491 2491 . . 2491 2495 + BlsI GCNGC 2493 2492 . . 2491 2495 + BisI GCNGC 2492 2493 . . 2491 2495 + TseI GCWGC 2491 2494 . . 2491 2495 - BbvI GCAGC 2478 2482 . . 2541 2546 + SfeI* CTRYAG 2541 2545 . . 2547 2550 + SetI ASST 2550 2546 . . 2547 2550 + CviJI RGCY 2548 2548 . . 2547 2550 + AluI AGCT 2548 2548 . . 2548 2552 + BlsI GCNGC 2550 2549 . . 2548 2552 + BisI GCNGC 2549 2550 . . 2548 2552 + TseI GCWGC 2548 2551 . . 2548 2552 - BbvI GCAGC 2535 2539 . . 2579 2582 + Csp6I GTAC 2579 2581 . . 2579 2582 + RsaI GTAC 2580 2580 . . 2581 2584 + SetI ASST 2584 2580 . . 2594 2597 + TspEI AATT 2593 2597 . . 2611 2616 + Hpy166II GTNNAC 2613 2613 . . 2611 2616 + HpaI GTTAAC 2613 2613 . . 2611 2616 + HindII GTYRAC 2613 2613 . . 2612 2615 + MseI TTAA 2612 2614 . . 2620 2625 + ApoI RAATTY 2620 2624 . . 2621 2624 + TspEI AATT 2620 2624 . . 2625 2634 + MslI CAYNNNNRTG 2629 2629 . . 2625 2628 + FatI CATG 2624 2628 . . 2625 2628 + NlaIII CATG 2628 2624 . . 2625 2628 + CviAII CATG 2625 2627 . . 2623 2627 - TspDTI ATGAA 2613 2611 . . 2631 2634 + FatI CATG 2630 2634 . . 2631 2634 + NlaIII CATG 2634 2630 . . 2631 2634 + CviAII CATG 2631 2633 . . 2635 2639 + TauI GCSGC 2638 2635 . . 2635 2639 + BlsI GCNGC 2637 2636 . . 2635 2639 + BisI GCNGC 2636 2637 . . 2635 2638 - AciI CCGC 2635 2637 . . 2643 2646 + TspEI AATT 2642 2646 . . 2653 2656 + SetI ASST 2656 2652 . . 2653 2656 + CviJI RGCY 2654 2654 . . 2653 2656 + AluI AGCT 2654 2654 . . 2660 2664 + Hpy188I TCNGA 2662 2661 . . 2663 2666 + DpnI GATC 2664 2664 . . 2663 2666 + BstKTI GATC 2665 2663 . . 2663 2666 + MboI GATC 2662 2666 . . 2666 2671 + NdeI CATATG 2667 2669 . . 2672 2676 + Tsp45I GTSAC 2671 2676 . . 2672 2676 + MaeIII GTNAC 2671 2676 . . 2703 2708 + SacI GAGCTC 2707 2703 . . 2703 2708 + Ecl136II GAGCTC 2705 2705 . . 2703 2708 + SduI GDGCHC 2707 2703 . . 2703 2708 + HgiJII* GRGCYC 2707 2703 . . 2703 2708 + HgiAI* GWGCWC 2707 2703 . . 2704 2707 + SetI ASST 2707 2703 . . 2704 2707 + CviJI RGCY 2705 2705 . . 2704 2707 + AluI AGCT 2705 2705 . . 2715 2720 + TatI WGTACW 2715 2719 . . 2715 2720 + Bsp1407I TGTACA 2715 2719 . . 2716 2719 + Csp6I GTAC 2716 2718 . . 2716 2719 + RsaI GTAC 2717 2717 . . 2722 2727 + HaeII RGCGCY 2726 2722 . . 2723 2726 + Hin6I GCGC 2723 2725 . . 2723 2726 + GlaI GCGC 2724 2724 . . 2723 2726 + HhaI GCGC 2725 2723 . . 2727 2736 + MslI CAYNNNNRTG 2731 2731 . . 2727 2730 + FatI CATG 2726 2730 . . 2727 2730 + NlaIII CATG 2730 2726 . . 2727 2730 + CviAII CATG 2727 2729 . . 2732 2737 + BsmI GAATGC 2738 2736 . . 2735 2738 + CviRI* TGCA 2736 2736 . . 2742 2745 + SetI ASST 2745 2741 . . 2742 2745 + CviJI RGCY 2743 2743 . . 2742 2745 + AluI AGCT 2743 2743 . . 2744 2747 + MaeI CTAG 2744 2746 . . 2752 2755 + SetI ASST 2755 2751 . . 2750 2754 - Hin4II* CCTTC 2744 2743 . . 2765 2768 + CviRI* TGCA 2766 2766 . . 2779 2783 + MboII GAAGA 2791 2790 . . 2784 2788 + TspGWI ACGGA 2799 2797 . . 2788 2793 + SspI AATATT 2790 2790 . . 2796 2799 + CviJI RGCY 2797 2797 . . 2798 2801 + MaeI CTAG 2798 2800 . . 2801 2805 + MaeIII GTNAC 2800 2805 . . 2817 2821 + BsrI ACTGG 2822 2820 . . 2818 2823 + Eco57MI CTGRAG 2839 2837 . . 2818 2823 + GsuI CTGGAG 2839 2837 . . 2821 2824 - MnlI CCTC 2814 2813 . . 2826 2829 + SetI ASST 2829 2825 . . 2826 2829 + CviJI RGCY 2827 2827 . . 2826 2829 + AluI AGCT 2827 2827 . . 2831 2834 + TaqI TCGA 2831 2833 . . 2833 2838 + ApoI RAATTY 2833 2837 . . 2834 2837 + TspEI AATT 2833 2837 . . 2837 2840 + MseI TTAA 2837 2839 . . 2841 2850 + XmnI GAANNNNTTC 2845 2845 . . 2848 2852 - TspDTI ATGAA 2838 2836 . . 2856 2860 + TfiI GAWTC 2856 2859 . . 2856 2860 + HinfI GANTC 2856 2859 . . 2859 2864 + XbaI TCTAGA 2859 2863 . . 2859 2864 + Hpy178III* TCNNGA 2860 2862 . . 2860 2863 + MaeI CTAG 2860 2862 . . 2869 2872 + MseI TTAA 2869 2871 . . 2875 2879 + HinfI GANTC 2875 2878 . . 2875 2879 - PleI GAGTC 2869 2870 . . 2875 2879 - MlyI GAGTC 2869 2869 . . 2890 2893 + MseI TTAA 2890 2892 . . 2895 2899 + Tsp4CI* ACNGT 2897 2896 . . 2903 2906 + CviRI* TGCA 2904 2904 . . 2920 2925 + SspI AATATT 2922 2922 . . 2926 2930 + BccI CCATC 2934 2935 . . 2930 2934 + DdeI CTNAG 2930 2933 . . 2946 2949 + Csp6I GTAC 2946 2948 . . 2946 2949 + RsaI GTAC 2947 2947 . . 2967 2972 + PsiI TTATAA 2969 2969 . . 2964 2969 - TsoI TARCCA 2954 2952 . . 2987 2990 + SetI ASST 2990 2986 . . 2987 2990 + CviJI RGCY 2988 2988 . . 2987 2990 + AluI AGCT 2988 2988 . . 2989 2994 + Eco57MI CTGRAG 3010 3008 . . 2989 2994 + Eco57I CTGAAG 3010 3008 . . 2991 2995 + MboII GAAGA 3003 3002 . . 2991 2996 + BbvII* GAAGAC 2998 3002 . . 2995 2998 + TaiI ACGT 2998 2994 . . 2995 2998 + SetI ASST 2998 2994 . . 2995 2998 + MaeII ACGT 2995 2997 . . 2997 3001 + MaeIII GTNAC 2996 3001 . . 3011 3016 + Hpy178III* TCNNGA 3012 3014 . . 3011 3016 + BspHI TCATGA 3011 3015 . . 3012 3015 + FatI CATG 3011 3015 . . 3012 3015 + NlaIII CATG 3015 3011 . . 3012 3015 + CviAII CATG 3012 3014 . . 3013 3017 + TspDTI ATGAA 3028 3026 . . 3021 3026 + AhaIII* TTTAAA 3023 3023 . . 3022 3025 + MseI TTAA 3022 3024 . . 3030 3034 + MboII GAAGA 3042 3041 . . 3031 3041 + FalI AAGNNNNNCTT 3022 3017 3054 3049 3033 3038 + BsmI GAATGC 3039 3037 . . 3031 3041 - FalI AAGNNNNNCTT 3054 3049 3022 3017 3042 3046 - Hin4II* CCTTC 3036 3035 . . 3064 3068 - MboII GAAGA 3056 3055 . . 3070 3073 + MseI TTAA 3070 3072 . . 3075 3079 + Tsp4CI* ACNGT 3077 3076 . . 3078 3082 + Tsp45I GTSAC 3077 3082 . . 3078 3082 + MaeIII GTNAC 3077 3082 . . 3082 3087 + NdeI CATATG 3083 3085 . . 3086 3089 + CviRI* TGCA 3087 3087 . . 3096 3099 + CviJI RGCY 3097 3097 . . 3097 3101 + BlsI GCNGC 3099 3098 . . 3097 3101 + BisI GCNGC 3098 3099 . . 3097 3101 + TseI GCWGC 3097 3100 . . 3097 3101 - BbvI GCAGC 3084 3088 . . 3108 3111 + SetI ASST 3111 3107 . . 3110 3114 + MaeIII GTNAC 3109 3114 . . 3117 3120 + SetI ASST 3120 3116 . . 3118 3122 + Hin4II* CCTTC 3128 3127 . . 3121 3126 + Hpy178III* TCNNGA 3122 3124 . . 3110 3122 - BarI GAAGNNNNNNTAC 3134 3129 3102 3097 3127 3130 + MseI TTAA 3127 3129 . . 3134 3138 + TspDTI ATGAA 3149 3147 . . 3148 3152 + TspDTI ATGAA 3163 3161 . . 3160 3163 + MaeI CTAG 3160 3162 . . 3176 3179 + TaiI ACGT 3179 3175 . . 3176 3179 + SetI ASST 3179 3175 . . 3176 3179 + MaeII ACGT 3176 3178 . . 3194 3198 - HphI GGTGA 3186 3185 . . 3234 3237 + SetI ASST 3237 3233 . . 3242 3246 + TfiI GAWTC 3242 3245 . . 3242 3246 + HinfI GANTC 3242 3245 . . 3269 3272 + SetI ASST 3272 3268 . . 3269 3272 + CviJI RGCY 3270 3270 . . 3269 3272 + AluI AGCT 3270 3270 . . 3271 3274 + MaeI CTAG 3271 3273 . . 3282 3285 + TspEI AATT 3281 3285 . . 3292 3296 + TfiI GAWTC 3292 3295 . . 3292 3296 + HinfI GANTC 3292 3295 . . 3295 3300 + Hpy178III* TCNNGA 3296 3298 . . 3303 3308 + Hpy178III* TCNNGA 3304 3306 . . 3307 3310 + DpnI GATC 3308 3308 . . 3307 3310 + BstKTI GATC 3309 3307 . . 3307 3310 + MboI GATC 3306 3310 . . 3312 3317 + XbaI TCTAGA 3312 3316 . . 3312 3317 + Hpy178III* TCNNGA 3313 3315 . . 3309 3313 - MboII GAAGA 3301 3300 . . 3313 3316 + MaeI CTAG 3313 3315 . . 3317 3320 + TspEI AATT 3316 3320 . . 3327 3331 + BmgT120I GGNCC 3328 3329 . . 3327 3331 + AvaII GGWCC 3327 3330 . . 3327 3331 + AsuI* GGNCC 3327 3330 . . 3329 3332 + SetI ASST 3332 3328 . . 3334 3337 + TspEI AATT 3333 3337 . . 3338 3342 + MaeIII GTNAC 3337 3342 . . 3352 3356 + Tsp45I GTSAC 3351 3356 . . 3352 3356 + MaeIII GTNAC 3351 3356 . . 3360 3363 + SetI ASST 3363 3359 . . 3362 3366 + MaeIII GTNAC 3361 3366 . . 3358 3362 - Hin4II* CCTTC 3352 3351 . . 3382 3386 + MaeIII GTNAC 3381 3386 . . 3386 3390 + DdeI CTNAG 3386 3389 . . 3389 3392 + SetI ASST 3392 3388 . . 3386 3390 - BspCNI CTCAG 3378 3376 . . 3386 3390 - BseMII CTCAG 3377 3375 . . 3390 3394 + HphI GGTGA 3402 3401 . . 3388 3391 - MnlI CCTC 3381 3380 . . 3398 3402 - BsmAI GTCTC 3392 3396 . . 3397 3407 - BsaXI ACNNNNNCTCC 3419 3416 3389 3386 3398 3409 - PpiI GAACNNNNNCTC 3421 3416 3389 3384 3397 3409 - AloI GAACNNNNNNTCC 3421 3416 3389 3384 3411 3417 + PpuMI RGGWCCY 3412 3415 . . 3411 3417 + DraII RGGNCCY 3412 3415 . . 3411 3416 + NlaIV GGNNCC 3413 3413 . . 3412 3416 + BmgT120I GGNCC 3413 3414 . . 3412 3416 + AvaII GGWCC 3412 3415 . . 3412 3416 + AsuI* GGNCC 3412 3415 . . 3420 3425 + AflIII ACRYGT 3420 3424 . . 3421 3426 + BtrI CACGTC 3423 3423 . . 3422 3425 + TaiI ACGT 3425 3421 . . 3422 3425 + SetI ASST 3425 3421 . . 3422 3425 + MaeII ACGT 3422 3424 . . 3431 3436 + SfeI* CTRYAG 3431 3435 . . 3433 3437 + Tsp4CI* ACNGT 3435 3434 . . 3434 3440 + TspRI CASTGNN 3440 3431 . . 3445 3448 + SetI ASST 3448 3444 . . 3445 3448 + CviJI RGCY 3446 3446 . . 3445 3448 + AluI AGCT 3446 3446 . . 3447 3451 + DdeI CTNAG 3447 3450 . . 3468 3472 + TfiI GAWTC 3468 3471 . . 3468 3472 + HinfI GANTC 3468 3471 . . 3481 3486 + ApoI RAATTY 3481 3485 . . 3482 3485 + TspEI AATT 3481 3485 . . 3484 3488 - TspDTI ATGAA 3474 3472 . . 3488 3493 + Hpy178III* TCNNGA 3489 3491 . . 3490 3493 + TaqI TCGA 3490 3492 . . 3505 3509 + Tsp4CI* ACNGT 3507 3506 . . 3506 3511 + SfeI* CTRYAG 3506 3510 . . 3516 3525 + MslI CAYNNNNRTG 3520 3520 . . 3525 3529 + PleI GAGTC 3533 3534 . . 3525 3529 + MlyI GAGTC 3534 3534 . . 3525 3529 + HinfI GANTC 3525 3528 . . 3527 3532 + Hpy166II GTNNAC 3529 3529 . . 3527 3532 + AccI GTMKAC 3528 3530 . . 3541 3554 + AjuI GAANNNNNNNTTGG 3533 3528 3565 3560 3568 3571 + MseI TTAA 3568 3570 . . 3565 3570 - TsoI TARCCA 3555 3553 . . 3580 3585 + Hpy178III* TCNNGA 3581 3583 . . 3589 3593 + FauI CCCGC 3597 3599 . . 3590 3593 + AciI CCGC 3590 3592 . . 3592 3602 + MwoI GCNNNNNNNGC 3598 3595 . . 3592 3602 + BstAPI GCANNNNNTGC 3598 3595 . . 3598 3602 + BlsI GCNGC 3600 3599 . . 3598 3602 + BisI GCNGC 3599 3600 . . 3598 3602 + TseI GCWGC 3598 3601 . . 3600 3603 + CviRI* TGCA 3601 3601 . . 3598 3602 - BbvI GCAGC 3585 3589 . . 3620 3624 + TspDTI ATGAA 3635 3633 . . 3634 3638 + HphI GGTGA 3646 3645 . . 3638 3642 + TspDTI ATGAA 3653 3651 . . 3651 3656 + Hpy166II GTNNAC 3653 3653 . . 3651 3656 + HpaI GTTAAC 3653 3653 . . 3651 3656 + HindII GTYRAC 3653 3653 . . 3652 3655 + MseI TTAA 3652 3654 . . 3656 3662 + TspRI CASTGNN 3662 3653 . . 3683 3687 + TspDTI ATGAA 3698 3696 . . 3690 3695 + AhaIII* TTTAAA 3692 3692 . . 3691 3694 + MseI TTAA 3691 3693 . . 3701 3704 + TaqI TCGA 3701 3703 . . 3721 3726 - TsoI TARCCA 3711 3709 . . 3730 3735 + ApoI RAATTY 3730 3734 . . 3731 3734 + TspEI AATT 3730 3734 . . 3734 3739 + Hpy178III* TCNNGA 3735 3737 . . 3734 3739 + BspHI TCATGA 3734 3738 . . 3735 3738 + FatI CATG 3734 3738 . . 3735 3738 + NlaIII CATG 3738 3734 . . 3735 3738 + CviAII CATG 3735 3737 . . 3736 3740 + TspDTI ATGAA 3751 3749 . . 3733 3737 - TspDTI ATGAA 3723 3721 . . 3741 3746 + NspI RCATGY 3745 3741 . . 3741 3746 + Cac8I GCNNGC 3743 3743 . . 3741 3746 + SphI GCATGC 3745 3741 . . 3742 3745 + FatI CATG 3741 3745 . . 3742 3745 + NlaIII CATG 3745 3741 . . 3742 3745 + CviAII CATG 3742 3744 . . 3750 3753 + CviJI RGCY 3751 3751 . . 3746 3751 - Eco57MI CTGRAG 3731 3729 . . 3746 3751 - Eco57I CTGAAG 3731 3729 . . 3758 3761 + TaqI TCGA 3758 3760 . . 3758 3763 + Hpy178III* TCNNGA 3759 3761 . . 3761 3766 + XhoII RGATCY 3761 3765 . . 3761 3766 + BglII AGATCT 3761 3765 . . 3762 3765 + DpnI GATC 3763 3763 . . 3762 3765 + BstKTI GATC 3764 3762 . . 3762 3765 + MboI GATC 3761 3765 . . 3767 3770 + TaqI TCGA 3767 3769 . . 3764 3768 - MboII GAAGA 3756 3755 . . 3777 3782 + AhaIII* TTTAAA 3779 3779 . . 3778 3781 + MseI TTAA 3778 3780 . . 3788 3791 + SetI ASST 3791 3787 . . 3788 3791 + CviJI RGCY 3789 3789 . . 3788 3791 + AluI AGCT 3789 3789 . . 3793 3798 + AhaIII* TTTAAA 3795 3795 . . 3794 3797 + MseI TTAA 3794 3796 . . 3798 3803 + ApoI RAATTY 3798 3802 . . 3799 3802 + TspEI AATT 3798 3802 . . 3805 3809 + HphI GGTGA 3817 3816 . . 3824 3827 + TspEI AATT 3823 3827 . . 3838 3841 + MseI TTAA 3838 3840 . . 3842 3846 + TspDTI ATGAA 3857 3855 . . 3844 3849 + ApoI RAATTY 3844 3848 . . 3845 3848 + TspEI AATT 3844 3848 . . 3850 3854 - SfaNI GCATC 3840 3844 . . 3865 3870 + ApoI RAATTY 3865 3869 . . 3866 3869 + TspEI AATT 3865 3869 . . 3877 3882 + Hpy166II GTNNAC 3879 3879 . . 3877 3882 + HindII GTYRAC 3879 3879 . . 3880 3884 + HinfI GANTC 3880 3883 . . 3880 3884 - PleI GAGTC 3874 3875 . . 3880 3884 - MlyI GAGTC 3874 3874 . . 3886 3891 + EcoT22I ATGCAT 3890 3886 . . 3887 3890 + CviRI* TGCA 3888 3888 . . 3896 3901 + ApoI RAATTY 3896 3900 . . 3897 3900 + TspEI AATT 3896 3900 . . 3902 3905 + MseI TTAA 3902 3904 . . 3913 3918 + Eco57MI CTGRAG 3934 3932 . . 3913 3918 + Eco57I CTGAAG 3934 3932 . . 3921 3925 + Hpy188I TCNGA 3923 3922 . . 3926 3929 + TaiI ACGT 3929 3925 . . 3926 3929 + SetI ASST 3929 3925 . . 3926 3929 + MaeII ACGT 3926 3928 . . 3938 3941 + MseI TTAA 3938 3940 . . 3944 3947 + SetI ASST 3947 3943 . . 3950 3955 + TsoI TARCCA 3966 3964 . . 3961 3965 + FauI CCCGC 3969 3971 . . 3962 3965 + AciI CCGC 3962 3964 . . 3959 3963 - BslFI GGGAC 3944 3948 . . 3963 3966 + FnuDII* CGCG 3964 3964 . . 3967 3970 + MseI TTAA 3967 3969 . . 3970 3973 + TspEI AATT 3969 3973 . . 3975 3987 + BarI GAAGNNNNNNTAC 3967 3962 3999 3994 3984 3987 + Csp6I GTAC 3984 3986 . . 3984 3987 + RsaI GTAC 3985 3985 . . 4004 4007 + TaqI TCGA 4004 4006 . . 4008 4011 - AciI CCGC 4008 4010 . . 4006 4011 - BsrBI CCGCTC 4008 4008 . . 4014 4018 + HphI GGTGA 4026 4025 . . 4018 4021 + SetI ASST 4021 4017 . . 4018 4021 + CviJI RGCY 4019 4019 . . 4018 4021 + AluI AGCT 4019 4019 . . 4035 4038 + TspEI AATT 4034 4038 . . 4039 4043 + MboII GAAGA 4051 4050 . . 4039 4044 + BbvII* GAAGAC 4046 4050 . . 4039 4048 + XmnI GAANNNNTTC 4043 4043 . . 4042 4051 + PshAI GACNNNNGTC 4046 4046 . . 4043 4046 + TaiI ACGT 4046 4042 . . 4043 4046 + SetI ASST 4046 4042 . . 4043 4046 + MaeII ACGT 4043 4045 . . 4049 4053 - BslFI GGGAC 4034 4038 . . 4054 4057 + TspEI AATT 4053 4057 . . 4059 4063 + TfiI GAWTC 4059 4062 . . 4059 4063 + HinfI GANTC 4059 4062 . . 4068 4071 + TspEI AATT 4067 4071 . . 4074 4077 + CviJI RGCY 4075 4075 . . 4079 4084 + Hpy178III* TCNNGA 4080 4082 . . 4089 4093 + Tsp4CI* ACNGT 4091 4090 . . 4095 4099 + MboII GAAGA 4107 4106 . . 4109 4120 + BdaI TGANNNNNNTCA 4098 4096 4132 4130 4110 4119 + XmnI GAANNNNTTC 4114 4114 . . 4109 4120 - BdaI TGANNNNNNTCA 4132 4130 4098 4096 4122 4126 + MaeIII GTNAC 4121 4126 . . 4130 4133 + TaqI TCGA 4130 4132 . . 4149 4153 + TfiI GAWTC 4149 4152 . . 4149 4153 + HinfI GANTC 4149 4152 . . 4155 4158 + CviJI RGCY 4156 4156 . . 4170 4173 + TspEI AATT 4169 4173 . . 4187 4190 + CviJI RGCY 4188 4188 . . 4188 4193 + Cac8I GCNNGC 4190 4190 . . 4204 4216 + BarI GAAGNNNNNNTAC 4196 4191 4228 4223 4206 4209 + SetI ASST 4209 4205 . . 4206 4209 + CviJI RGCY 4207 4207 . . 4206 4209 + AluI AGCT 4207 4207 . . 4208 4213 + Eco57MI CTGRAG 4229 4227 . . 4208 4213 + GsuI CTGGAG 4229 4227 . . 4213 4216 + Csp6I GTAC 4213 4215 . . 4213 4216 + RsaI GTAC 4214 4214 . . 4226 4231 + Hpy178III* TCNNGA 4227 4229 . . 4231 4234 + TspEI AATT 4230 4234 . . 4239 4242 + SetI ASST 4242 4238 . . 4239 4242 + CviJI RGCY 4240 4240 . . 4239 4242 + AluI AGCT 4240 4240 . . 4244 4247 + TaiI ACGT 4247 4243 . . 4244 4247 + SetI ASST 4247 4243 . . 4244 4247 + MaeII ACGT 4244 4246 . . 4247 4252 + BclI TGATCA 4247 4251 . . 4248 4251 + DpnI GATC 4249 4249 . . 4248 4251 + BstKTI GATC 4250 4248 . . 4248 4251 + MboI GATC 4247 4251 . . 4273 4277 + BssKI CCNGG 4272 4277 . . 4273 4277 + BseBI CCWGG 4274 4275 . . 4273 4278 + SecI* CCNNGG 4273 4277 . . 4273 4277 + ScrFI CCNGG 4274 4275 . . 4273 4277 + EcoRII CCWGG 4272 4277 . . 4278 4282 + MboII GAAGA 4290 4289 . . 4281 4285 - BccI CCATC 4275 4276 . . 4287 4290 + TspEI AATT 4286 4290 . . 4304 4307 + TspEI AATT 4303 4307 . . 4309 4313 + HinfI GANTC 4309 4312 . . 4309 4313 - PleI GAGTC 4303 4304 . . 4309 4313 - MlyI GAGTC 4303 4303 . . 4323 4327 + BccI CCATC 4331 4332 . . 4339 4344 + SpeI ACTAGT 4339 4343 . . 4340 4343 + MaeI CTAG 4340 4342 . . 4344 4347 + CviRI* TGCA 4345 4345 . . 4369 4372 - MnlI CCTC 4362 4361 . . 4372 4375 + CviJI RGCY 4373 4373 . . 4373 4379 + EspI* GCTNAGC 4374 4377 . . 4374 4378 + DdeI CTNAG 4374 4377 . . 4377 4380 + SetI ASST 4380 4376 . . 4377 4380 + CviJI RGCY 4378 4378 . . 4377 4380 + AluI AGCT 4378 4378 . . 4381 4385 + Tsp4CI* ACNGT 4383 4382 . . 4392 4395 + TspEI AATT 4391 4395 . . 4394 4399 + PsiI TTATAA 4396 4396 . . 4400 4403 + TspEI AATT 4399 4403 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 29 4387 # # AccI: # [GTMKAC] # 136 214 888 918 2260 # # AciI: # [CCGC] # 46 59 86 270 372 408 # 955 999 1221 # # AclI: # [AACGTT] # 94 4322 # # AflIII: # [ACRYGT] # 996 3420 # # AgeI: # [ACCGGT] # 837 3579 # # AhaIII*: # [TTTAAA] # 16 87 621 669 731 2292 # # AjuI: # [GAANNNNNNNTTGG] # 9 23 32 32 32 851 # 3437 # # AlfI: # [GCANNNNNNTGC] # 0 0 0 0 34 34 # 235 406 3707 # # AloI: # [GAACNNNNNNTCC] # 32 995 3389 # # AluI: # [AGCT] # 13 15 33 38 38 51 # 57 62 84 103 106 138 # 152 156 160 161 176 188 # 208 230 256 282 297 312 # 343 757 # # ApoI: # [RAATTY] # 9 21 31 39 46 49 # 68 90 108 117 124 151 # 180 213 230 249 338 520 # 577 608 648 # # AsuI*: # [GGNCC] # 85 275 456 873 1004 1723 # # AsuII: # [TTCGAA] # 155 275 1227 2759 # # AvaI: # [CYCGRG] # 929 1010 2477 # # AvaII: # [GGWCC] # 85 275 456 1004 2596 # # AvrII: # [CCTAGG] # 1761 2655 # # BalI: # [TGGCCA] # 1954 2462 # # BarI: # [GAAGNNNNNNTAC] # 32 32 32 188 197 833 # 3102 # # BbvI: # [GCAGC] # 57 248 501 549 831 2230 # # BbvII*: # [GAAGAC] # 370 1048 2998 # # BccI: # [CCATC] # 7 19 56 68 74 85 # 115 354 615 822 860 1341 # # Bce83I*: # [CTTGAG] # 1952 2464 # # BciVI: # [GTATCC] # 144 4272 # # BclI: # [TGATCA] # 168 169 4079 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 34 34 34 34 # 151 284 616 1103 2126 # # BetI*: # [WCCGGW] # 338 499 3579 # # BglII: # [AGATCT] # 655 3761 # # BinI*: # [GGATC] # 25 51 174 562 1114 2490 # # BisI: # [GCNGC] # 57 60 67 86 87 202 # 462 501 817 2077 # # BlsI: # [GCNGC] # 57 61 67 86 87 202 # 462 501 816 2077 # # BmeT110I: # [CYCGRG] # 930 1010 2476 # # BmgT120I: # [GGNCC] # 85 275 457 873 1003 1723 # # BplI: # [GAGNNNNNCTC] # 0 0 32 1360 3024 # # Bpu10I: # [CCTNAGC] # 1025 1298 2093 # # BsaBI: # [GATNNNNATC] # 3 75 167 298 1411 2462 # # BsaXI: # [ACNNNNNCTCC] # 30 30 116 997 3243 # # BseBI: # [CCWGG] # 142 765 1150 2359 # # BseGI: # [GGATG] # 45 126 1608 2637 # # BseMII: # [CTCAG] # 289 315 437 563 1039 1773 # # BseRI: # [GAGGAG] # 451 3965 # # BseYI: # [CCCAGC] # 1884 2532 # # BsgI: # [GTGCAG] # 2114 2302 # # BsiI*: # [CACGAG] # 979 3437 # # BsiYI*: # [CCNNNNNNNGG] # 51 91 98 165 236 246 # 465 850 2214 # # BslFI: # [GGGAC] # 90 382 1234 2710 # # BsmAI: # [GTCTC] # 74 182 198 522 766 1024 # 1650 # # BsmI: # [GAATGC] # 301 432 1377 2306 # # Bsp1407I: # [TGTACA] # 1701 2715 # # BspCNI: # [CTCAG] # 291 313 437 562 1038 1775 # # BspHI: # [TCATGA] # 539 682 723 2472 # # BspMI: # [ACCTGC] # 1097 3319 # # BsrBI: # [CCGCTC] # 408 4008 # # BsrI: # [ACTGG] # 1594 2822 # # BssKI: # [CCNGG] # 144 763 1150 2359 # # BstAPI: # [GCANNNNNTGC] # 818 1089 2509 # # BstKTI: # [GATC] # 9 12 38 166 171 174 # 193 455 458 486 493 551 # 566 644 # # BstXI: # [CCANNNNNNTGG] # 2111 2305 # # BtrI: # [CACGTC] # 993 3423 # # BtsI: # [GCAGTG] # 263 4153 # # Cac8I: # [GCNNGC] # 4 209 226 266 447 748 # 1079 1437 # # Cfr10I: # [RCCGGY] # 252 585 3579 # # CfrI: # [YGGCCR] # 1952 2464 # # Csp6I: # [GTAC] # 30 84 120 137 157 173 # 203 229 230 1038 2015 # # CspCI: # [CAANNNNNGTGG] # 35 1236 3145 # # CviAII: # [CATG] # 6 7 7 20 64 72 # 96 152 168 170 218 265 # 283 285 319 417 470 674 # 723 # # CviJI: # [RGCY] # 5 6 10 11 13 15 # 17 19 21 26 30 32 # 32 33 38 38 38 39 # 40 43 43 44 50 51 # 54 55 56 56 57 62 # 62 63 69 81 102 106 # 109 123 133 136 143 160 # 161 173 176 230 247 256 # 260 287 305 # # CviRI*: # [TGCA] # 30 43 43 52 71 80 # 104 138 157 159 183 213 # 264 287 333 385 432 457 # 471 514 # # DdeI: # [CTNAG] # 20 42 61 82 241 292 # 312 437 447 456 492 607 # 927 # # DpnI: # [GATC] # 9 12 38 167 170 174 # 193 455 458 486 493 551 # 566 644 # # DraII: # [RGGNCCY] # 1004 3412 # # DsaI*: # [CCRYGG] # 391 2008 2017 # # Ecl136II: # [GAGCTC] # 227 1711 2478 # # Eco47III: # [AGCGCT] # 1208 3208 # # Eco57I: # [CTGAAG] # 203 482 506 633 721 1871 # # Eco57MI: # [CTGRAG] # 171 187 203 295 430 462 # 506 721 1441 # # EcoRI: # [GAATTC] # 49 2125 2242 # # EcoRII: # [CCWGG] # 144 763 1150 2359 # # EcoRV: # [GATATC] # 1347 3069 # # EcoT22I: # [ATGCAT] # 526 1537 2353 # # Esp3I: # [CGTCTC] # 522 3894 # # EspI*: # [GCTNAGC] # 42 4374 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 32 32 32 64 890 1362 # 2004 # # FatI: # [CATG] # 6 7 7 20 64 72 # 96 152 168 170 218 264 # 283 285 319 417 470 675 # 723 # # FauI: # [CCCGC] # 372 447 3597 # # FnuDII*: # [CGCG] # 368 452 3596 # # FokI: # [GGATG] # 25 133 1628 2630 # # GlaI: # [GCGC] # 1208 1516 1692 # # GsaI: # [CCCAGC] # 1888 2528 # # GsuI: # [CTGGAG] # 187 430 1390 2409 # # HaeII: # [RGCGCY] # 1210 1516 1690 # # HaeIII: # [GGCC] # 348 1606 2462 # # HgaI: # [GACGC] # 1069 3347 # # HgiAI*: # [GWGCWC] # 227 1709 2480 # # HgiCI*: # [GGYRCC] # 29 4387 # # HgiJII*: # [GRGCYC] # 227 1709 2480 # # HhaI: # [GCGC] # 1209 1516 1691 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 32 253 # 658 1154 2255 # # Hin4II*: # [CCTTC] # 82 92 224 249 292 317 # 486 586 1024 1064 # # Hin6I: # [GCGC] # 1207 1516 1693 # # HindII: # [GTYRAC] # 226 351 399 537 1040 1863 # # HinfI: # [GANTC] # 19 50 57 70 90 107 # 160 162 176 179 211 238 # 285 355 367 375 443 488 # 584 # # HpaI: # [GTTAAC] # 763 1040 2613 # # HpaII: # [CCGG] # 247 253 338 3578 # # HphI: # [GGTGA] # 60 171 209 216 244 390 # 445 2681 # # Hpy166II: # [GTNNAC] # 124 136 215 226 371 399 # 537 547 916 945 # # Hpy178III*: # [TCNNGA] # 8 8 9 24 26 29 # 40 40 45 78 92 107 # 110 111 147 148 152 154 # 174 176 189 204 233 240 # 321 331 367 379 474 # # Hpy188I: # [TCNGA] # 79 96 243 245 335 412 # 420 493 832 1261 # # Hpy99I: # [CGWCG] # 1063 3353 # # KpnI: # [GGTACC] # 33 4383 # # Ksp632I*: # [CTCTTC] # 774 1073 2569 # # MaeI: # [CTAG] # 42 47 54 62 76 111 # 165 300 389 471 498 511 # 663 1027 # # MaeII: # [ACGT] # 94 117 172 181 201 246 # 504 1345 1556 # # MaeIII: # [GTNAC] # 10 14 20 21 32 66 # 81 129 196 228 295 518 # 577 655 740 834 # # MboI: # [GATC] # 9 12 38 168 169 174 # 193 455 458 486 493 551 # 566 644 # # MboII: # [GAAGA] # 14 39 56 108 126 183 # 212 245 285 295 381 455 # 927 1090 # # MlyI: # [GAGTC] # 113 220 238 340 429 665 # 758 1653 # # MnlI: # [CCTC] # 3 7 33 41 44 54 # 67 126 126 150 318 505 # 567 611 783 981 # # MseI: # [TTAA] # 16 21 29 32 36 39 # 44 48 57 64 84 87 # 132 159 225 226 238 266 # 321 351 441 449 1051 # # MslI: # [CAYNNNNRTG] # 102 337 520 789 896 1772 # # MwoI: # [GCNNNNNNNGC] # 25 56 124 358 650 818 # 2385 # # NcoI: # [CCATGG] # 391 2008 2017 # # NdeI: # [CATATG] # 416 1333 2667 # # NlaIII: # [CATG] # 6 7 7 20 64 72 # 96 152 168 170 218 268 # 283 285 319 417 470 671 # 723 # # NlaIV: # [GGNNCC] # 31 319 382 1003 2681 # # NruI: # [TCGCGA] # 368 4048 # # NspI: # [RCATGY] # 268 671 835 2642 # # PasI: # [CCCWGGG] # 1914 2502 # # PflMI: # [CCANNNNNTGG] # 819 3597 # # PleI: # [GAGTC] # 113 219 238 341 429 664 # 759 1653 # # PpiI: # [GAACNNNNNCTC] # 32 995 3389 # # PpuMI: # [RGGWCCY] # 1004 3412 # # PshAI: # [GACNNNNGTC] # 370 4046 # # PsiI: # [TTATAA] # 20 786 1427 2183 # # RsaI: # [GTAC] # 31 84 120 137 157 173 # 202 229 230 1038 2015 # # SacI: # [GAGCTC] # 227 1709 2480 # # SalI: # [GTCGAC] # 349 4067 # # SapI: # [GCTCTTC] # 774 1073 2569 # # ScrFI: # [CCNGG] # 142 765 1150 2359 # # SduI: # [GDGCHC] # 227 1709 2480 # # SecI*: # [CCNNGG] # 1 22 103 143 152 391 # 869 870 1865 # # SetI: # [ASST] # 5 8 9 10 12 13 # 15 18 18 18 20 21 # 23 25 29 29 31 31 # 32 33 33 34 35 36 # 38 40 42 51 57 57 # 58 59 59 60 72 74 # 74 76 80 97 103 113 # 128 129 130 133 133 138 # 138 156 161 163 167 206 # 249 294 343 # # SfaNI: # [GCATC] # 130 134 393 576 761 942 # 1480 # # SfeI*: # [CTRYAG] # 75 156 228 890 910 2157 # # SmlI: # [CTYRAG] # 929 1550 1937 # # SpeI: # [ACTAGT] # 77 4339 # # SphI: # [GCATGC] # 268 671 3477 # # SspI: # [AATATT] # 132 1494 2790 # # StyI: # [CCWWGG] # 22 256 391 869 870 2008 # # TaiI: # [ACGT] # 97 117 169 181 201 246 # 504 1345 1556 # # TaqI: # [TCGA] # 9 57 107 126 155 195 # 211 237 249 275 286 331 # 345 363 370 441 659 # # TatI: # [WGTACW] # 173 186 1701 2356 # # TauI: # [GCSGC] # 62 86 269 1778 2221 # # TfiI: # [GAWTC] # 50 70 90 162 176 267 # 386 443 496 584 591 1101 # # TseI: # [GCWGC] # 57 261 501 549 818 2230 # # TsoI: # [TARCCA] # 56 156 243 244 255 291 # 417 450 601 1703 # # Tsp45I: # [GTSAC] # 21 66 274 406 655 834 # 1065 1095 # # Tsp4CI*: # [ACNGT] # 33 36 72 132 180 292 # 348 358 584 2381 # # TspDTI: # [ATGAA] # 4 14 18 25 28 30 # 35 45 51 58 72 98 # 106 112 119 121 140 161 # 190 225 233 256 275 301 # 311 367 462 559 # # TspEI: # [AATT] # 6 8 9 10 12 14 # 15 17 17 17 17 17 # 19 19 20 21 21 22 # 23 23 25 27 27 29 # 30 31 31 33 35 39 # 39 49 50 56 58 61 # 61 65 68 73 75 88 # 93 101 102 112 134 148 # 153 155 156 180 191 227 # 249 269 321 448 # # TspGWI: # [ACGGA] # 632 1617 2167 # # TspRI: # [CASTGNN] # 222 232 263 459 754 2486 # # TstI: # [CACNNNNNNTCC] # 32 32 576 1468 2308 # # XbaI: # [TCTAGA] # 453 627 1104 2232 # # XhoI: # [CTCGAG] # 929 3487 # # XhoII: # [RGATCY] # 655 1843 1918 # # XmnI: # [GAANNNNTTC] # 71 79 302 578 716 1198 # 1472 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4416 # Reported_sequences: 1 # Reported_hitcount: 992 #---------------------------------------