Restriction Fragments for VPS8/YAL002W



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The currently selected gene/sequence is :

VPS8/YAL002W on chromosome I from coordinates 143707 to 147531.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19623.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 16 + CviJI RGCY 14 14 . . 13 16 + HaeIII GGCC 14 14 . . 25 35 + Hin4I GAYNNNNNVTC 16 11 48 43 27 32 + EcoP15I CAGCAG 57 59 . . 29 39 + MwoI GCNNNNNNNGC 35 32 . . 31 36 + XhoII RGATCY 31 35 . . 31 36 + BglII AGATCT 31 35 . . 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 35 + DpnI GATC 33 33 . . 32 35 + BstKTI GATC 34 32 . . 32 35 + MboI GATC 31 35 . . 25 35 - Hin4I GAYNNNNNVTC 48 43 16 11 35 38 + MaeI CTAG 35 37 . . 38 42 + SfaNI GCATC 47 51 . . 40 45 + ClaI ATCGAT 41 43 . . 41 44 + TaqI TCGA 41 43 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 52 57 + VspI ATTAAT 53 55 . . 53 56 + MseI TTAA 53 55 . . 76 79 + MaeI CTAG 76 78 . . 79 84 + ApoI RAATTY 79 83 . . 80 83 + TspEI AATT 79 83 . . 86 89 + DpnI GATC 87 87 . . 86 89 + BstKTI GATC 88 86 . . 86 89 + MboI GATC 85 89 . . 90 95 + Hpy166II GTNNAC 92 92 . . 90 95 + BssNAI GTATAC 92 92 . . 90 95 + AccI GTMKAC 91 93 . . 98 101 + TspEI AATT 97 101 . . 100 103 + MseI TTAA 100 102 . . 111 115 + BsrI ACTGG 116 114 . . 115 119 + SfaNI GCATC 124 128 . . 119 122 + MaeI CTAG 119 121 . . 124 128 - MboII GAAGA 116 115 . . 126 130 - TspDTI ATGAA 116 114 . . 133 143 + BsaXI ACNNNNNCTCC 123 120 153 150 133 137 + BceAI ACGGC 149 151 . . 135 140 + NlaIV GGNNCC 137 137 . . 135 140 + HgiCI* GGYRCC 135 139 . . 138 141 + SetI ASST 141 137 . . 139 142 + MnlI CCTC 149 148 . . 151 155 + MboII GAAGA 163 162 . . 157 161 + BmgT120I GGNCC 158 159 . . 157 161 + AvaII GGWCC 157 160 . . 157 161 + AsuI* GGNCC 157 160 . . 154 158 - BccI CCATC 148 149 . . 171 174 + SetI ASST 174 170 . . 171 174 + CviJI RGCY 172 172 . . 171 174 + AluI AGCT 172 172 . . 176 181 + PstI CTGCAG 180 176 . . 176 181 + SfeI* CTRYAG 176 180 . . 177 180 + CviRI* TGCA 178 178 . . 178 183 + BtsI GCAGTG 185 183 . . 179 185 + TspRI CASTGNN 185 176 . . 183 187 + Tsp45I GTSAC 182 187 . . 183 187 + MaeIII GTNAC 182 187 . . 190 193 + CviJI RGCY 191 191 . . 190 195 + NlaIV GGNNCC 192 192 . . 193 197 + Hpy188I TCNGA 195 194 . . 196 200 + TfiI GAWTC 196 199 . . 196 200 + HinfI GANTC 196 199 . . 199 203 - TspGWI ACGGA 189 187 . . 205 209 + BsmAI GTCTC 210 214 . . 209 212 + FatI CATG 208 212 . . 209 212 + NlaIII CATG 212 208 . . 209 212 + CviAII CATG 209 211 . . 220 223 + MseI TTAA 220 222 . . 225 230 + AflIII ACRYGT 225 229 . . 226 237 + TstI CACNNNNNNTCC 217 212 249 244 226 231 + BsaAI YACGTR 228 228 . . 227 230 + TaiI ACGT 230 226 . . 227 230 + SetI ASST 230 226 . . 227 230 + MaeII ACGT 227 229 . . 236 240 + BccI CCATC 244 245 . . 240 244 + BssKI CCNGG 239 244 . . 240 244 + BseBI CCWGG 241 242 . . 240 245 + SecI* CCNNGG 240 244 . . 240 244 + ScrFI CCNGG 241 242 . . 240 244 + EcoRII CCWGG 239 244 . . 237 241 - FokI GGATG 223 227 . . 237 241 - BseGI GGATG 236 234 . . 242 247 - TaqII CACCCA 232 230 . . 255 261 + DraII RGGNCCY 256 259 . . 256 261 + SduI GDGCHC 260 256 . . 256 261 + Bsp120I GGGCCC 256 260 . . 256 261 + NlaIV GGNNCC 258 258 . . 256 260 + BmgT120I GGNCC 257 258 . . 256 261 + HgiJII* GRGCYC 260 256 . . 256 261 + BseSI GKGCMC 260 256 . . 256 260 + AsuI* GGNCC 256 259 . . 256 261 + ApaI GGGCCC 260 256 . . 254 257 - MnlI CCTC 247 246 . . 257 260 + CviJI RGCY 258 258 . . 257 260 + HaeIII GGCC 258 258 . . 257 261 + BmgT120I GGNCC 258 259 . . 257 261 + AsuI* GGNCC 257 260 . . 261 266 + BsaAI YACGTR 263 263 . . 261 266 + PmaCI CACGTG 263 263 . . 262 265 + TaiI ACGT 265 261 . . 262 265 + SetI ASST 265 261 . . 262 265 + MaeII ACGT 262 264 . . 270 275 + BciVI GTATCC 281 280 . . 273 278 + MmeI TCCRAC 298 296 . . 273 277 + Hpy188I TCNGA 275 274 . . 279 282 + CviJI RGCY 280 280 . . 282 287 + BsaAI YACGTR 284 284 . . 283 286 + TaiI ACGT 286 282 . . 283 286 + SetI ASST 286 282 . . 283 286 + MaeII ACGT 283 285 . . 297 302 + SduI GDGCHC 301 297 . . 297 302 + BseSI GKGCMC 301 297 . . 294 298 - BccI CCATC 288 289 . . 310 314 + FokI GGATG 323 327 . . 310 314 + BseGI GGATG 316 314 . . 311 321 + Hin4I GAYNNNNNVTC 302 297 334 329 311 315 - SfaNI GCATC 301 305 . . 316 319 + Csp6I GTAC 316 318 . . 316 319 + RsaI GTAC 317 317 . . 326 329 + MseI TTAA 326 328 . . 328 331 + TspEI AATT 327 331 . . 330 335 + PsiI TTATAA 332 332 . . 335 339 + TspDTI ATGAA 350 348 . . 344 347 + CviRI* TGCA 345 345 . . 355 358 + MaeI CTAG 355 357 . . 364 367 + SetI ASST 367 363 . . 370 374 + Hpy188I TCNGA 372 371 . . 371 375 + DdeI CTNAG 371 374 . . 374 380 + PpuMI RGGWCCY 375 378 . . 374 380 + DraII RGGNCCY 375 378 . . 371 375 - BspCNI CTCAG 363 361 . . 371 375 - BseMII CTCAG 362 360 . . 375 380 + NlaIV GGNNCC 377 377 . . 375 379 + BmgT120I GGNCC 376 377 . . 375 379 + AvaII GGWCC 375 378 . . 375 379 + AsuI* GGNCC 375 378 . . 373 376 - MnlI CCTC 366 365 . . 379 383 + Hin4II* CCTTC 389 388 . . 404 410 + TspRI CASTGNN 410 401 . . 403 407 - BsrI ACTGG 403 401 . . 413 418 + MfeI CAATTG 413 417 . . 414 417 + TspEI AATT 413 417 . . 420 425 + NdeI CATATG 421 423 . . 427 431 + Hpy188I TCNGA 429 428 . . 434 437 + Csp6I GTAC 434 436 . . 430 434 - BccI CCATC 424 425 . . 434 437 + RsaI GTAC 435 435 . . 439 442 + FatI CATG 438 442 . . 439 442 + NlaIII CATG 442 438 . . 439 442 + CviAII CATG 439 441 . . 433 443 - BaeI ACNNNNGTAYC 458 453 425 420 444 447 + SetI ASST 447 443 . . 444 447 + CviJI RGCY 445 445 . . 444 447 + AluI AGCT 445 445 . . 445 449 + BlsI GCNGC 447 446 . . 445 449 + BisI GCNGC 446 447 . . 445 449 + TseI GCWGC 445 448 . . 449 452 + MnlI CCTC 459 458 . . 445 449 - BbvI GCAGC 432 436 . . 460 465 + BetI* WCCGGW 460 464 . . 461 464 + HpaII CCGG 461 463 . . 457 461 - BsmAI GTCTC 451 455 . . 457 462 - Eco31I GGTCTC 451 455 . . 471 476 + EcoT22I ATGCAT 475 471 . . 472 475 + CviRI* TGCA 473 473 . . 488 491 + TaiI ACGT 491 487 . . 488 491 + SetI ASST 491 487 . . 488 491 + MaeII ACGT 488 490 . . 514 518 + Hpy188I TCNGA 516 515 . . 520 530 + BsiYI* CCNNNNNNNGG 526 523 . . 520 530 + PflMI CCANNNNNTGG 526 523 . . 543 548 + Cac8I GCNNGC 545 545 . . 547 557 + MwoI GCNNNNNNNGC 553 550 . . 547 557 + BglI GCCNNNNNGGC 553 550 . . 552 557 + Cfr10I RCCGGY 552 556 . . 553 556 + HpaII CCGG 553 555 . . 565 574 + MslI CAYNNNNRTG 569 569 . . 568 573 + ClaI ATCGAT 569 571 . . 569 572 + TaqI TCGA 569 571 . . 577 580 + FatI CATG 576 580 . . 577 580 + NlaIII CATG 580 576 . . 577 580 + CviAII CATG 577 579 . . 580 585 + Hpy166II GTNNAC 582 582 . . 614 619 + SduI GDGCHC 618 614 . . 614 619 + HgiAI* GWGCWC 618 614 . . 617 622 - NmeAIII GCCGAG 597 595 . . 627 630 + CviJI RGCY 628 628 . . 651 654 + SetI ASST 654 650 . . 671 675 + Tsp4CI* ACNGT 673 672 . . 674 678 + MaeIII GTNAC 673 678 . . 680 684 + MboII GAAGA 692 691 . . 680 693 + AjuI GAANNNNNNNTTGG 672 667 704 699 685 688 + CviJI RGCY 686 686 . . 680 685 - Ksp632I* CTCTTC 675 678 . . 683 686 - MnlI CCTC 676 675 . . 706 709 + TspEI AATT 705 709 . . 716 721 + ApoI RAATTY 716 720 . . 717 720 + TspEI AATT 716 720 . . 724 734 + Hin4I GAYNNNNNVTC 715 710 747 742 727 732 + Hpy166II GTNNAC 729 729 . . 732 737 + Ksp632I* CTCTTC 738 741 . . 733 737 - MboII GAAGA 725 724 . . 737 742 + StyI CCWWGG 737 741 . . 737 742 + SecI* CCNNGG 737 741 . . 746 749 + TspEI AATT 745 749 . . 747 752 + VspI ATTAAT 748 750 . . 748 751 + MseI TTAA 748 750 . . 754 757 + SetI ASST 757 753 . . 765 769 + BsmAI GTCTC 770 774 . . 788 793 + ApoI RAATTY 788 792 . . 789 792 + TspEI AATT 788 792 . . 799 802 + TspEI AATT 798 802 . . 806 817 + BdaI TGANNNNNNTCA 795 793 829 827 809 814 + Hpy166II GTNNAC 811 811 . . 810 815 + TatI WGTACW 810 814 . . 811 814 + Csp6I GTAC 811 813 . . 811 814 + RsaI GTAC 812 812 . . 806 817 - BdaI TGANNNNNNTCA 829 827 795 793 852 857 + AflIII ACRYGT 852 856 . . 854 857 + TaiI ACGT 857 853 . . 854 857 + SetI ASST 857 853 . . 854 857 + MaeII ACGT 854 856 . . 870 875 + Hpy178III* TCNNGA 871 873 . . 877 881 + Tsp4CI* ACNGT 879 878 . . 883 895 + PsrI GAACNNNNNNTAC 875 870 907 902 887 890 + MnlI CCTC 897 896 . . 888 892 + DdeI CTNAG 888 891 . . 888 892 + BspCNI CTCAG 901 899 . . 888 892 + BseMII CTCAG 902 900 . . 891 896 + TatI WGTACW 891 895 . . 892 895 + Csp6I GTAC 892 894 . . 892 895 + RsaI GTAC 893 893 . . 897 902 + ApoI RAATTY 897 901 . . 898 901 + TspEI AATT 897 901 . . 904 907 + MaeI CTAG 904 906 . . 908 913 + Hpy178III* TCNNGA 909 911 . . 916 919 + SetI ASST 919 915 . . 916 919 + CviJI RGCY 917 917 . . 916 919 + AluI AGCT 917 917 . . 926 929 + FatI CATG 925 929 . . 926 929 + NlaIII CATG 929 925 . . 926 929 + CviAII CATG 926 928 . . 924 928 - TspDTI ATGAA 914 912 . . 930 934 + HinfI GANTC 930 933 . . 930 934 - PleI GAGTC 924 925 . . 930 934 - MlyI GAGTC 924 924 . . 938 942 + Tsp4CI* ACNGT 940 939 . . 944 948 + BssKI CCNGG 943 948 . . 944 948 + BseBI CCWGG 945 946 . . 944 949 + SecI* CCNNGG 944 948 . . 944 948 + ScrFI CCNGG 945 946 . . 944 948 + EcoRII CCWGG 943 948 . . 958 962 - TspGWI ACGGA 948 946 . . 971 976 + ApoI RAATTY 971 975 . . 972 975 + TspEI AATT 971 975 . . 991 995 - MboII GAAGA 983 982 . . 993 997 - TspDTI ATGAA 983 981 . . 997 1001 + Hpy188I TCNGA 999 998 . . 1016 1019 + AciI CCGC 1016 1018 . . 1018 1023 + Cac8I GCNNGC 1020 1020 . . 1018 1023 + BmtI GCTAGC 1022 1018 . . 1018 1023 + NheI GCTAGC 1018 1022 . . 1019 1022 + MaeI CTAG 1019 1021 . . 1020 1025 + TsoI TARCCA 1036 1034 . . 1021 1024 + CviJI RGCY 1022 1022 . . 1029 1034 + Hpy178III* TCNNGA 1030 1032 . . 1033 1038 + ApoI RAATTY 1033 1037 . . 1034 1037 + TspEI AATT 1033 1037 . . 1038 1041 + CviRI* TGCA 1039 1039 . . 1042 1046 + TfiI GAWTC 1042 1045 . . 1042 1046 + HinfI GANTC 1042 1045 . . 1055 1065 + BsiYI* CCNNNNNNNGG 1061 1058 . . 1055 1065 + PflMI CCANNNNNTGG 1061 1058 . . 1071 1085 + XcmI CCANNNNNNNNNTGG 1078 1077 . . 1073 1076 + SetI ASST 1076 1072 . . 1073 1076 + CviJI RGCY 1074 1074 . . 1073 1076 + AluI AGCT 1074 1074 . . 1090 1093 + MseI TTAA 1090 1092 . . 1091 1096 + TsoI TARCCA 1107 1105 . . 1104 1114 + BsiYI* CCNNNNNNNGG 1110 1107 . . 1104 1114 + PflMI CCANNNNNTGG 1110 1107 . . 1106 1109 + TspEI AATT 1105 1109 . . 1118 1121 + CviRI* TGCA 1119 1119 . . 1129 1132 + FatI CATG 1128 1132 . . 1129 1132 + NlaIII CATG 1132 1128 . . 1129 1132 + CviAII CATG 1129 1131 . . 1136 1141 + Hpy178III* TCNNGA 1137 1139 . . 1139 1144 + XhoII RGATCY 1139 1143 . . 1140 1143 + DpnI GATC 1141 1141 . . 1140 1143 + BstKTI GATC 1142 1140 . . 1140 1143 + MboI GATC 1139 1143 . . 1140 1144 - BinI* GGATC 1134 1135 . . 1147 1150 + MseI TTAA 1147 1149 . . 1161 1165 + Hpy188I TCNGA 1163 1162 . . 1164 1168 + TfiI GAWTC 1164 1167 . . 1164 1168 + HinfI GANTC 1164 1167 . . 1167 1172 + Hpy178III* TCNNGA 1168 1170 . . 1179 1182 + DpnI GATC 1180 1180 . . 1179 1182 + BstKTI GATC 1181 1179 . . 1179 1182 + MboI GATC 1178 1182 . . 1179 1183 - BinI* GGATC 1173 1174 . . 1185 1188 + SetI ASST 1188 1184 . . 1193 1198 + SspI AATATT 1195 1195 . . 1211 1215 + MboII GAAGA 1223 1222 . . 1223 1226 + SetI ASST 1226 1222 . . 1227 1232 + Hpy166II GTNNAC 1229 1229 . . 1227 1232 + HpaI GTTAAC 1229 1229 . . 1227 1232 + HindII GTYRAC 1229 1229 . . 1228 1231 + MseI TTAA 1228 1230 . . 1243 1248 + AhaIII* TTTAAA 1245 1245 . . 1244 1247 + MseI TTAA 1244 1246 . . 1248 1251 + TspEI AATT 1247 1251 . . 1255 1260 + ApoI RAATTY 1255 1259 . . 1256 1259 + TspEI AATT 1255 1259 . . 1270 1274 + Hpy188I TCNGA 1272 1271 . . 1282 1285 + MseI TTAA 1282 1284 . . 1314 1317 + CviRI* TGCA 1315 1315 . . 1310 1315 - TaqII CACCCA 1300 1298 . . 1324 1328 - TspDTI ATGAA 1314 1312 . . 1330 1334 + TfiI GAWTC 1330 1333 . . 1330 1334 + HinfI GANTC 1330 1333 . . 1340 1343 + CviRI* TGCA 1341 1341 . . 1344 1347 + SetI ASST 1347 1343 . . 1349 1353 + Tsp4CI* ACNGT 1351 1350 . . 1352 1357 + Hpy166II GTNNAC 1354 1354 . . 1355 1361 + TspRI CASTGNN 1361 1352 . . 1356 1360 + BsrI ACTGG 1361 1359 . . 1373 1376 + SetI ASST 1376 1372 . . 1373 1376 + CviJI RGCY 1374 1374 . . 1373 1376 + AluI AGCT 1374 1374 . . 1375 1378 + MaeI CTAG 1375 1377 . . 1387 1391 + TspGWI ACGGA 1402 1400 . . 1390 1394 + MboII GAAGA 1402 1401 . . 1390 1395 - Ksp632I* CTCTTC 1385 1388 . . 1395 1398 - MnlI CCTC 1388 1387 . . 1400 1404 + DdeI CTNAG 1400 1403 . . 1413 1418 + NlaIV GGNNCC 1415 1415 . . 1433 1438 + StyI CCWWGG 1433 1437 . . 1433 1438 + SecI* CCNNGG 1433 1437 . . 1437 1440 + CviJI RGCY 1438 1438 . . 1438 1442 + BlsI GCNGC 1440 1439 . . 1438 1442 + BisI GCNGC 1439 1440 . . 1438 1442 + TseI GCWGC 1438 1441 . . 1438 1442 - BbvI GCAGC 1425 1429 . . 1443 1449 + SauI* CCTNAGG 1444 1447 . . 1444 1448 + DdeI CTNAG 1444 1447 . . 1447 1450 + SetI ASST 1450 1446 . . 1454 1459 + PsiI TTATAA 1456 1456 . . 1486 1491 + Hpy166II GTNNAC 1488 1488 . . 1493 1496 + TspEI AATT 1492 1496 . . 1497 1502 + VspI ATTAAT 1498 1500 . . 1498 1501 + MseI TTAA 1498 1500 . . 1520 1523 + CviRI* TGCA 1521 1521 . . 1521 1525 + BlsI GCNGC 1523 1522 . . 1521 1525 + BisI GCNGC 1522 1523 . . 1521 1525 + BbvI GCAGC 1533 1537 . . 1521 1525 + TseI GCWGC 1521 1524 . . 1528 1532 - MboII GAAGA 1520 1519 . . 1540 1543 + MaeI CTAG 1540 1542 . . 1543 1547 + HinfI GANTC 1543 1546 . . 1543 1547 - PleI GAGTC 1537 1538 . . 1543 1547 - MlyI GAGTC 1537 1537 . . 1548 1551 + TspEI AATT 1547 1551 . . 1552 1556 + Hin4II* CCTTC 1562 1561 . . 1557 1562 + XbaI TCTAGA 1557 1561 . . 1557 1562 + Hpy178III* TCNNGA 1558 1560 . . 1558 1561 + MaeI CTAG 1558 1560 . . 1561 1566 + ZraI GACGTC 1563 1563 . . 1561 1566 + AcyI GRCGYC 1562 1564 . . 1561 1566 + AatII GACGTC 1565 1561 . . 1562 1565 + TaiI ACGT 1565 1561 . . 1562 1565 + SetI ASST 1565 1561 . . 1562 1565 + MaeII ACGT 1562 1564 . . 1576 1584 + AlwNI CAGNNNCTG 1581 1578 . . 1581 1586 + Hpy178III* TCNNGA 1582 1584 . . 1582 1587 + Eco57MI CTGRAG 1603 1601 . . 1582 1587 + GsuI CTGGAG 1603 1601 . . 1595 1598 + TspEI AATT 1594 1598 . . 1599 1603 - Hin4II* CCTTC 1593 1592 . . 1608 1611 + FnuDII* CGCG 1609 1609 . . 1609 1612 - AciI CCGC 1609 1611 . . 1633 1638 + SspI AATATT 1635 1635 . . 1644 1649 + StyI CCWWGG 1644 1648 . . 1644 1649 + SecI* CCNNGG 1644 1648 . . 1648 1652 + BinI* GGATC 1656 1657 . . 1649 1652 + DpnI GATC 1650 1650 . . 1649 1652 + BstKTI GATC 1651 1649 . . 1649 1652 + MboI GATC 1648 1652 . . 1654 1658 + MaeIII GTNAC 1653 1658 . . 1657 1660 + TaiI ACGT 1660 1656 . . 1657 1660 + SetI ASST 1660 1656 . . 1657 1660 + MaeII ACGT 1657 1659 . . 1662 1665 + TspEI AATT 1661 1665 . . 1669 1673 - BsrI ACTGG 1669 1667 . . 1668 1673 - BfiI ACTGGG 1663 1662 . . 1681 1684 + SetI ASST 1684 1680 . . 1682 1686 + BmgT120I GGNCC 1683 1684 . . 1682 1686 + AvaII GGWCC 1682 1685 . . 1682 1686 + AsuI* GGNCC 1682 1685 . . 1689 1692 + TspEI AATT 1688 1692 . . 1703 1707 + DdeI CTNAG 1703 1706 . . 1708 1713 + BseRI GAGGAG 1723 1721 . . 1702 1708 - Bpu10I CCTNAGC 1703 1706 . . 1708 1711 - MnlI CCTC 1701 1700 . . 1713 1718 + ApoI RAATTY 1713 1717 . . 1714 1717 + TspEI AATT 1713 1717 . . 1716 1721 + AhaIII* TTTAAA 1718 1718 . . 1717 1720 + MseI TTAA 1717 1719 . . 1725 1728 + TspEI AATT 1724 1728 . . 1729 1733 + MboII GAAGA 1741 1740 . . 1729 1734 + BbvII* GAAGAC 1736 1740 . . 1735 1738 + MseI TTAA 1735 1737 . . 1743 1746 + FatI CATG 1742 1746 . . 1743 1746 + NlaIII CATG 1746 1742 . . 1743 1746 + CviAII CATG 1743 1745 . . 1747 1750 + MseI TTAA 1747 1749 . . 1765 1774 + BsaBI GATNNNNATC 1769 1769 . . 1771 1774 + DpnI GATC 1772 1772 . . 1771 1774 + BstKTI GATC 1773 1771 . . 1771 1774 + MboI GATC 1770 1774 . . 1777 1781 - BceAI ACGGC 1762 1764 . . 1801 1804 + TspEI AATT 1800 1804 . . 1808 1811 + TaqI TCGA 1808 1810 . . 1797 1809 - PsrI GAACNNNNNNTAC 1821 1816 1789 1784 1815 1820 + VspI ATTAAT 1816 1818 . . 1816 1819 + MseI TTAA 1816 1818 . . 1835 1840 + XhoII RGATCY 1835 1839 . . 1835 1840 + BglII AGATCT 1835 1839 . . 1836 1839 + DpnI GATC 1837 1837 . . 1836 1839 + BstKTI GATC 1838 1836 . . 1836 1839 + MboI GATC 1835 1839 . . 1842 1853 + BdaI TGANNNNNNTCA 1831 1829 1865 1863 1843 1852 + BsaBI GATNNNNATC 1847 1847 . . 1842 1853 - BdaI TGANNNNNNTCA 1865 1863 1831 1829 1857 1867 + BsiYI* CCNNNNNNNGG 1863 1860 . . 1859 1865 + TspRI CASTGNN 1865 1856 . . 1858 1862 - BsrI ACTGG 1858 1856 . . 1857 1862 - BfiI ACTGGG 1852 1851 . . 1864 1869 + Hpy166II GTNNAC 1866 1866 . . 1887 1892 + TsoI TARCCA 1903 1901 . . 1908 1911 + DpnI GATC 1909 1909 . . 1908 1911 + BstKTI GATC 1910 1908 . . 1908 1911 + MboI GATC 1907 1911 . . 1910 1914 - MboII GAAGA 1902 1901 . . 1918 1922 + BmgT120I GGNCC 1919 1920 . . 1918 1922 + AvaII GGWCC 1918 1921 . . 1918 1922 + AsuI* GGNCC 1918 1921 . . 1921 1924 + MnlI CCTC 1931 1930 . . 1922 1927 + SmlI CTYRAG 1922 1926 . . 1927 1930 + Csp6I GTAC 1927 1929 . . 1922 1927 - Bce83I* CTTGAG 1907 1905 . . 1927 1930 + RsaI GTAC 1928 1928 . . 1929 1932 + SetI ASST 1932 1928 . . 1930 1933 + MnlI CCTC 1940 1939 . . 1932 1937 + Hpy178III* TCNNGA 1933 1935 . . 1954 1959 + SnaBI TACGTA 1956 1956 . . 1954 1959 + BsaAI YACGTR 1956 1956 . . 1955 1958 + TaiI ACGT 1958 1954 . . 1955 1958 + SetI ASST 1958 1954 . . 1955 1958 + MaeII ACGT 1955 1957 . . 1957 1961 + MaeIII GTNAC 1956 1961 . . 1960 1963 + TaiI ACGT 1963 1959 . . 1960 1963 + SetI ASST 1963 1959 . . 1960 1963 + MaeII ACGT 1960 1962 . . 1972 1976 + BsrI ACTGG 1977 1975 . . 1976 1981 + Cac8I GCNNGC 1978 1978 . . 1987 1998 - CspCI CAANNNNNGTGG 2011 2009 1976 1974 2006 2014 + DraIII CACNNNGTG 2011 2008 . . 2005 2009 - HphI GGTGA 1997 1996 . . 2014 2024 + Hin4I GAYNNNNNVTC 2005 2000 2037 2032 2062 2065 + CviJI RGCY 2063 2063 . . 2069 2079 + BsiYI* CCNNNNNNNGG 2075 2072 . . 2069 2079 + PflMI CCANNNNNTGG 2075 2072 . . 2077 2082 + CfrI YGGCCR 2077 2081 . . 2078 2081 + CviJI RGCY 2079 2079 . . 2078 2081 + HaeIII GGCC 2079 2079 . . 2081 2085 + Hpy99I CGWCG 2085 2080 . . 2083 2086 + TaqI TCGA 2083 2085 . . 2079 2083 - BceAI ACGGC 2064 2066 . . 2106 2111 + NdeI CATATG 2107 2109 . . 2118 2123 + Hpy178III* TCNNGA 2119 2121 . . 2122 2126 + MboII GAAGA 2134 2133 . . 2125 2129 + MboII GAAGA 2137 2136 . . 2129 2132 + CviJI RGCY 2130 2130 . . 2130 2135 + Cac8I GCNNGC 2132 2132 . . 2125 2130 - Ksp632I* CTCTTC 2120 2123 . . 2125 2131 - SapI GCTCTTC 2120 2123 . . 2150 2153 + SetI ASST 2153 2149 . . 2148 2152 - HphI GGTGA 2140 2139 . . 2164 2167 + TaqI TCGA 2164 2166 . . 2166 2170 + TfiI GAWTC 2166 2169 . . 2166 2170 + HinfI GANTC 2166 2169 . . 2169 2173 + Hpy188I TCNGA 2171 2170 . . 2176 2179 + SetI ASST 2179 2175 . . 2181 2185 + DdeI CTNAG 2181 2184 . . 2185 2190 + BsrDI GCAATG 2192 2190 . . 2195 2199 + TspDTI ATGAA 2210 2208 . . 2209 2213 - HgaI GACGC 2198 2203 . . 2219 2222 + MseI TTAA 2219 2221 . . 2235 2238 + FatI CATG 2234 2238 . . 2235 2238 + NlaIII CATG 2238 2234 . . 2235 2238 + CviAII CATG 2235 2237 . . 2241 2244 + CviRI* TGCA 2242 2242 . . 2242 2246 + SfaNI GCATC 2251 2255 . . 2248 2253 - MmeI TCCRAC 2229 2227 . . 2261 2264 + TspEI AATT 2260 2264 . . 2287 2290 + DpnI GATC 2288 2288 . . 2287 2290 + BstKTI GATC 2289 2287 . . 2287 2290 + MboI GATC 2286 2290 . . 2298 2302 + FokI GGATG 2311 2315 . . 2298 2302 + BseGI GGATG 2304 2302 . . 2299 2303 - SfaNI GCATC 2289 2293 . . 2305 2309 + TspDTI ATGAA 2320 2318 . . 2319 2324 + ApoI RAATTY 2319 2323 . . 2320 2323 + TspEI AATT 2319 2323 . . 2323 2327 + Hpy188I TCNGA 2325 2324 . . 2338 2341 + Csp6I GTAC 2338 2340 . . 2338 2341 + RsaI GTAC 2339 2339 . . 2346 2349 + CviRI* TGCA 2347 2347 . . 2349 2352 + TspEI AATT 2348 2352 . . 2353 2357 - TspDTI ATGAA 2343 2341 . . 2358 2361 + CviRI* TGCA 2359 2359 . . 2355 2360 - BsrDI GCAATG 2354 2352 . . 2362 2367 + SpeI ACTAGT 2362 2366 . . 2363 2366 + MaeI CTAG 2363 2365 . . 2380 2383 + MnlI CCTC 2390 2389 . . 2384 2387 + TspEI AATT 2383 2387 . . 2390 2393 + MseI TTAA 2390 2392 . . 2400 2405 + TsoI TARCCA 2416 2414 . . 2406 2409 + CviJI RGCY 2407 2407 . . 2409 2412 + MnlI CCTC 2419 2418 . . 2411 2414 + TaqI TCGA 2411 2413 . . 2423 2426 + MseI TTAA 2423 2425 . . 2430 2435 + NdeI CATATG 2431 2433 . . 2438 2441 + MnlI CCTC 2448 2447 . . 2436 2441 - BseRI GAGGAG 2427 2425 . . 2441 2444 + MaeI CTAG 2441 2443 . . 2451 2454 + SetI ASST 2454 2450 . . 2459 2463 + TspDTI ATGAA 2474 2472 . . 2460 2471 + BdaI TGANNNNNNTCA 2449 2447 2483 2481 2462 2467 + ApoI RAATTY 2462 2466 . . 2463 2466 + TspEI AATT 2462 2466 . . 2460 2471 - BdaI TGANNNNNNTCA 2483 2481 2449 2447 2475 2478 + SetI ASST 2478 2474 . . 2475 2478 + CviJI RGCY 2476 2476 . . 2475 2478 + AluI AGCT 2476 2476 . . 2478 2483 + Hpy178III* TCNNGA 2479 2481 . . 2479 2484 + Eco57MI CTGRAG 2500 2498 . . 2479 2484 + GsuI CTGGAG 2500 2498 . . 2482 2486 + PleI GAGTC 2490 2491 . . 2482 2486 + MlyI GAGTC 2491 2491 . . 2482 2486 + HinfI GANTC 2482 2485 . . 2506 2511 + Hpy178III* TCNNGA 2507 2509 . . 2520 2525 + ApoI RAATTY 2520 2524 . . 2521 2524 + TspEI AATT 2520 2524 . . 2529 2533 + BsrI ACTGG 2534 2532 . . 2547 2552 + ApoI RAATTY 2547 2551 . . 2548 2551 + TspEI AATT 2547 2551 . . 2592 2595 + Csp6I GTAC 2592 2594 . . 2592 2595 + RsaI GTAC 2593 2593 . . 2598 2604 + TspRI CASTGNN 2604 2595 . . 2597 2601 - BsrI ACTGG 2597 2595 . . 2602 2605 + Hin6I GCGC 2602 2604 . . 2602 2605 + GlaI GCGC 2603 2603 . . 2602 2605 + HhaI GCGC 2604 2602 . . 2624 2628 + DdeI CTNAG 2624 2627 . . 2633 2636 + TaqI TCGA 2633 2635 . . 2650 2654 + TspGWI ACGGA 2665 2663 . . 2653 2663 + Hin4I GAYNNNNNVTC 2644 2639 2676 2671 2659 2664 + BciVI GTATCC 2670 2669 . . 2653 2663 - Hin4I GAYNNNNNVTC 2676 2671 2644 2639 2694 2697 + SetI ASST 2697 2693 . . 2695 2699 + BssKI CCNGG 2694 2699 . . 2695 2699 + BseBI CCWGG 2696 2697 . . 2695 2699 + ScrFI CCNGG 2696 2697 . . 2695 2699 + EcoRII CCWGG 2694 2699 . . 2719 2723 + MaeIII GTNAC 2718 2723 . . 2723 2728 + Eco57MI CTGRAG 2744 2742 . . 2723 2728 + Eco57I CTGAAG 2744 2742 . . 2732 2735 + TaqI TCGA 2732 2734 . . 2732 2737 + Hpy178III* TCNNGA 2733 2735 . . 2734 2738 - BsmAI GTCTC 2728 2732 . . 2734 2739 - Esp3I CGTCTC 2728 2732 . . 2746 2749 + DpnI GATC 2747 2747 . . 2746 2749 + BstKTI GATC 2748 2746 . . 2746 2749 + MboI GATC 2745 2749 . . 2748 2752 - MboII GAAGA 2740 2739 . . 2751 2755 - MboII GAAGA 2743 2742 . . 2757 2762 + SmlI CTYRAG 2757 2761 . . 2757 2762 - Bce83I* CTTGAG 2742 2740 . . 2762 2766 + BinI* GGATC 2770 2771 . . 2763 2766 + DpnI GATC 2764 2764 . . 2763 2766 + BstKTI GATC 2765 2763 . . 2763 2772 + BsaBI GATNNNNATC 2767 2767 . . 2763 2766 + MboI GATC 2762 2766 . . 2771 2774 + TaqI TCGA 2771 2773 . . 2782 2786 + Tsp45I GTSAC 2781 2786 . . 2782 2786 + MaeIII GTNAC 2781 2786 . . 2780 2784 - HgaI GACGC 2769 2774 . . 2787 2790 + TspEI AATT 2786 2790 . . 2794 2798 + Hpy188I TCNGA 2796 2795 . . 2811 2815 - MboII GAAGA 2803 2802 . . 2813 2817 - TspDTI ATGAA 2803 2801 . . 2817 2822 + Hpy178III* TCNNGA 2818 2820 . . 2825 2831 + PfoI TCCNGGA 2825 2830 . . 2826 2830 + BssKI CCNGG 2825 2830 . . 2826 2830 + BseBI CCWGG 2827 2828 . . 2826 2830 + ScrFI CCNGG 2827 2828 . . 2826 2830 + EcoRII CCWGG 2825 2830 . . 2841 2852 + BdaI TGANNNNNNTCA 2830 2828 2864 2862 2842 2847 + BseRI GAGGAG 2857 2855 . . 2842 2845 - MnlI CCTC 2835 2834 . . 2847 2851 + HinfI GANTC 2847 2850 . . 2849 2853 + DdeI CTNAG 2849 2852 . . 2849 2853 + BspCNI CTCAG 2862 2860 . . 2849 2853 + BseMII CTCAG 2863 2861 . . 2845 2849 - BsmAI GTCTC 2839 2843 . . 2847 2851 - PleI GAGTC 2841 2842 . . 2847 2851 - MlyI GAGTC 2841 2841 . . 2841 2852 - BdaI TGANNNNNNTCA 2864 2862 2830 2828 2869 2874 + HindIII AAGCTT 2869 2873 . . 2870 2873 + SetI ASST 2873 2869 . . 2870 2873 + CviJI RGCY 2871 2871 . . 2870 2873 + AluI AGCT 2871 2871 . . 2900 2903 + SetI ASST 2903 2899 . . 2903 2906 + TaqI TCGA 2903 2905 . . 2914 2917 + MseI TTAA 2914 2916 . . 2919 2924 + ApoI RAATTY 2919 2923 . . 2920 2923 + TspEI AATT 2919 2923 . . 2930 2933 + TaqI TCGA 2930 2932 . . 2933 2936 + TspEI AATT 2932 2936 . . 2937 2949 + PsrI GAACNNNNNNTAC 2929 2924 2961 2956 2939 2943 + Tsp4CI* ACNGT 2941 2940 . . 2962 2971 + XmnI GAANNNNTTC 2966 2966 . . 2967 2971 + TfiI GAWTC 2967 2970 . . 2967 2971 + HinfI GANTC 2967 2970 . . 2975 2978 + CviJI RGCY 2976 2976 . . 2978 2981 + MseI TTAA 2978 2980 . . 2984 2987 + Csp6I GTAC 2984 2986 . . 2984 2987 + RsaI GTAC 2985 2985 . . 2986 2990 + Tsp4CI* ACNGT 2988 2987 . . 2992 2996 + DdeI CTNAG 2992 2995 . . 2992 2996 + BspCNI CTCAG 3005 3003 . . 2992 2996 + BseMII CTCAG 3006 3004 . . 3002 3006 + DdeI CTNAG 3002 3005 . . 3002 3006 - BspCNI CTCAG 2994 2992 . . 3002 3006 - BseMII CTCAG 2993 2991 . . 3007 3010 + SetI ASST 3010 3006 . . 3007 3010 + CviJI RGCY 3008 3008 . . 3007 3010 + AluI AGCT 3008 3008 . . 3014 3019 + ApoI RAATTY 3014 3018 . . 3015 3018 + TspEI AATT 3014 3018 . . 3018 3023 + Hpy178III* TCNNGA 3019 3021 . . 3030 3034 + TfiI GAWTC 3030 3033 . . 3030 3034 + HinfI GANTC 3030 3033 . . 3033 3038 + Hpy178III* TCNNGA 3034 3036 . . 3037 3041 + HinfI GANTC 3037 3040 . . 3037 3041 - PleI GAGTC 3031 3032 . . 3037 3041 - MlyI GAGTC 3031 3031 . . 3043 3046 + TspEI AATT 3042 3046 . . 3051 3054 + SetI ASST 3054 3050 . . 3051 3054 + CviJI RGCY 3052 3052 . . 3051 3054 + AluI AGCT 3052 3052 . . 3052 3056 + BlsI GCNGC 3054 3053 . . 3052 3056 + BisI GCNGC 3053 3054 . . 3052 3056 + TseI GCWGC 3052 3055 . . 3053 3058 + PstI CTGCAG 3057 3053 . . 3053 3058 + SfeI* CTRYAG 3053 3057 . . 3054 3057 + CviRI* TGCA 3055 3055 . . 3055 3059 + BlsI GCNGC 3057 3056 . . 3055 3059 + BisI GCNGC 3056 3057 . . 3055 3059 + BbvI GCAGC 3067 3071 . . 3055 3059 + TseI GCWGC 3055 3058 . . 3052 3056 - BbvI GCAGC 3039 3043 . . 3057 3060 + SetI ASST 3060 3056 . . 3057 3060 + CviJI RGCY 3058 3058 . . 3057 3060 + AluI AGCT 3058 3058 . . 3063 3066 + TspEI AATT 3062 3066 . . 3066 3071 + Hpy178III* TCNNGA 3067 3069 . . 3086 3089 + MseI TTAA 3086 3088 . . 3089 3092 + SetI ASST 3092 3088 . . 3091 3094 + MaeI CTAG 3091 3093 . . 3109 3112 + MseI TTAA 3109 3111 . . 3134 3138 + TspDTI ATGAA 3149 3147 . . 3136 3140 - Hin4II* CCTTC 3130 3129 . . 3166 3170 + TfiI GAWTC 3166 3169 . . 3166 3170 + HinfI GANTC 3166 3169 . . 3174 3179 + BseRI GAGGAG 3189 3187 . . 3174 3177 - MnlI CCTC 3167 3166 . . 3178 3184 + DraII RGGNCCY 3179 3182 . . 3179 3183 + BmgT120I GGNCC 3180 3181 . . 3179 3183 + AsuI* GGNCC 3179 3182 . . 3177 3180 - MnlI CCTC 3170 3169 . . 3180 3183 + CviJI RGCY 3181 3181 . . 3180 3183 + HaeIII GGCC 3181 3181 . . 3196 3199 + TspEI AATT 3195 3199 . . 3205 3210 + KpnI GGTACC 3209 3205 . . 3200 3205 - EcoP15I CAGCAG 3172 3174 . . 3205 3210 + NlaIV GGNNCC 3207 3207 . . 3205 3210 + HgiCI* GGYRCC 3205 3209 . . 3205 3210 + Acc65I GGTACC 3205 3209 . . 3206 3209 + Csp6I GTAC 3206 3208 . . 3206 3209 + RsaI GTAC 3207 3207 . . 3211 3221 - Hin4I GAYNNNNNVTC 3234 3229 3202 3197 3224 3229 - MmeI TCCRAC 3205 3203 . . 3245 3250 + Hpy178III* TCNNGA 3246 3248 . . 3248 3253 + BclI TGATCA 3248 3252 . . 3249 3252 + DpnI GATC 3250 3250 . . 3249 3252 + BstKTI GATC 3251 3249 . . 3249 3252 + MboI GATC 3248 3252 . . 3271 3275 + Hin4II* CCTTC 3281 3280 . . 3281 3285 + TspDTI ATGAA 3296 3294 . . 3312 3317 + Hpy178III* TCNNGA 3313 3315 . . 3332 3337 - TsoI TARCCA 3322 3320 . . 3337 3340 + SetI ASST 3340 3336 . . 3338 3342 + BmgT120I GGNCC 3339 3340 . . 3338 3342 + AvaII GGWCC 3338 3341 . . 3338 3342 + AsuI* GGNCC 3338 3341 . . 3345 3355 + BplI GAGNNNNNCTC 3336 3331 3368 3363 3350 3353 + MnlI CCTC 3360 3359 . . 3353 3357 + DdeI CTNAG 3353 3356 . . 3353 3357 + BspCNI CTCAG 3366 3364 . . 3353 3357 + BseMII CTCAG 3367 3365 . . 3354 3358 + Hpy188I TCNGA 3356 3355 . . 3345 3355 - BplI GAGNNNNNCTC 3368 3363 3336 3331 3361 3365 + TfiI GAWTC 3361 3364 . . 3357 3361 - Hin4II* CCTTC 3351 3350 . . 3361 3365 + HinfI GANTC 3361 3364 . . 3366 3369 + SetI ASST 3369 3365 . . 3373 3378 + EcoRI GAATTC 3373 3377 . . 3373 3378 + ApoI RAATTY 3373 3377 . . 3374 3377 + TspEI AATT 3373 3377 . . 3391 3395 - MboII GAAGA 3383 3382 . . 3390 3395 - BbvII* GAAGAC 3383 3387 . . 3399 3404 + Hpy178III* TCNNGA 3400 3402 . . 3400 3405 + SmlI CTYRAG 3400 3404 . . 3400 3405 + Bce83I* CTTGAG 3421 3419 . . 3403 3408 + SduI GDGCHC 3407 3403 . . 3403 3408 + HgiAI* GWGCWC 3407 3403 . . 3413 3416 + TaiI ACGT 3416 3412 . . 3413 3416 + SetI ASST 3416 3412 . . 3413 3416 + MaeII ACGT 3413 3415 . . 3421 3424 + MseI TTAA 3421 3423 . . 3424 3428 + TspDTI ATGAA 3439 3437 . . 3432 3437 + Hpy178III* TCNNGA 3433 3435 . . 3438 3443 + AhaIII* TTTAAA 3440 3440 . . 3439 3442 + MseI TTAA 3439 3441 . . 3464 3467 + MseI TTAA 3464 3466 . . 3471 3476 + PsiI TTATAA 3473 3473 . . 3476 3479 + TspEI AATT 3475 3479 . . 3484 3489 + XhoII RGATCY 3484 3488 . . 3484 3489 + BglII AGATCT 3484 3488 . . 3485 3488 + DpnI GATC 3486 3486 . . 3485 3488 + BstKTI GATC 3487 3485 . . 3485 3488 + MboI GATC 3484 3488 . . 3493 3497 + Hpy188I TCNGA 3495 3494 . . 3494 3498 + DdeI CTNAG 3494 3497 . . 3495 3506 + BdaI TGANNNNNNTCA 3484 3482 3518 3516 3494 3498 - BspCNI CTCAG 3486 3484 . . 3494 3498 - BseMII CTCAG 3485 3483 . . 3501 3505 + TfiI GAWTC 3501 3504 . . 3501 3505 + HinfI GANTC 3501 3504 . . 3495 3506 - BdaI TGANNNNNNTCA 3518 3516 3484 3482 3520 3524 + TfiI GAWTC 3520 3523 . . 3517 3520 - MnlI CCTC 3510 3509 . . 3520 3524 + HinfI GANTC 3520 3523 . . 3523 3527 - MboII GAAGA 3515 3514 . . 3531 3536 + XhoII RGATCY 3531 3535 . . 3531 3536 + BglII AGATCT 3531 3535 . . 3532 3535 + DpnI GATC 3533 3533 . . 3532 3535 + BstKTI GATC 3534 3532 . . 3532 3535 + MboI GATC 3531 3535 . . 3543 3547 + Tsp4CI* ACNGT 3545 3544 . . 3553 3558 + ApoI RAATTY 3553 3557 . . 3554 3557 + TspEI AATT 3553 3557 . . 3558 3562 + Hpy188I TCNGA 3560 3559 . . 3559 3563 + DdeI CTNAG 3559 3562 . . 3559 3563 - BspCNI CTCAG 3551 3549 . . 3559 3563 - BseMII CTCAG 3550 3548 . . 3565 3569 - Hin4II* CCTTC 3559 3558 . . 3585 3589 + Hpy99I CGWCG 3589 3584 . . 3593 3603 + MwoI GCNNNNNNNGC 3599 3596 . . 3602 3605 - AciI CCGC 3602 3604 . . 3602 3606 - FauI CCCGC 3595 3597 . . 3621 3624 + SetI ASST 3624 3620 . . 3621 3624 + CviJI RGCY 3622 3622 . . 3621 3624 + AluI AGCT 3622 3622 . . 3627 3632 + Hpy178III* TCNNGA 3628 3630 . . 3630 3635 + NlaIV GGNNCC 3632 3632 . . 3630 3634 + BmgT120I GGNCC 3631 3632 . . 3630 3634 + AvaII GGWCC 3630 3633 . . 3630 3634 + AsuI* GGNCC 3630 3633 . . 3646 3649 + MaeI CTAG 3646 3648 . . 3648 3651 + SetI ASST 3651 3647 . . 3648 3651 + CviJI RGCY 3649 3649 . . 3648 3651 + AluI AGCT 3649 3649 . . 3660 3665 + TatI WGTACW 3660 3664 . . 3660 3665 + Bsp1407I TGTACA 3660 3664 . . 3661 3664 + Csp6I GTAC 3661 3663 . . 3661 3664 + RsaI GTAC 3662 3662 . . 3665 3670 + HaeII RGCGCY 3669 3665 . . 3666 3669 + Hin6I GCGC 3666 3668 . . 3666 3669 + GlaI GCGC 3667 3667 . . 3666 3669 + HhaI GCGC 3668 3666 . . 3690 3695 + XhoII RGATCY 3690 3694 . . 3690 3695 + BglII AGATCT 3690 3694 . . 3691 3694 + DpnI GATC 3692 3692 . . 3691 3694 + BstKTI GATC 3693 3691 . . 3691 3694 + MboI GATC 3690 3694 . . 3722 3726 + Tsp45I GTSAC 3721 3726 . . 3722 3726 + MaeIII GTNAC 3721 3726 . . 3726 3731 + DsaI* CCRYGG 3726 3730 . . 3726 3731 + StyI CCWWGG 3726 3730 . . 3726 3731 + NcoI CCATGG 3726 3730 . . 3726 3731 + SecI* CCNNGG 3726 3730 . . 3727 3730 + FatI CATG 3726 3730 . . 3727 3730 + NlaIII CATG 3730 3726 . . 3727 3730 + CviAII CATG 3727 3729 . . 3723 3727 - HphI GGTGA 3715 3714 . . 3730 3733 + CviJI RGCY 3731 3731 . . 3735 3739 - HphI GGTGA 3727 3726 . . 3747 3750 + MnlI CCTC 3757 3756 . . 3749 3752 + TaqI TCGA 3749 3751 . . 3759 3762 + CviJI RGCY 3760 3760 . . 3759 3762 + HaeIII GGCC 3760 3760 . . 3759 3763 + BmgT120I GGNCC 3760 3761 . . 3759 3763 + AsuI* GGNCC 3759 3762 . . 3773 3777 + TspDTI ATGAA 3788 3786 . . 3776 3781 + SspI AATATT 3778 3778 . . 3787 3790 + MseI TTAA 3787 3789 . . 3789 3792 + TspEI AATT 3788 3792 . . 3799 3803 + TspGWI ACGGA 3814 3812 . . 3802 3806 + TfiI GAWTC 3802 3805 . . 3802 3806 + HinfI GANTC 3802 3805 . . #--------------------------------------- # # # AatII: # [GACGTC] # 1565 2260 # # Acc65I: # [GGTACC] # 620 3205 # # AccI: # [GTMKAC] # 91 3734 # # AciI: # [CCGC] # 223 593 1016 1993 # # AcyI: # [GRCGYC] # 1562 2263 # # AflIII: # [ACRYGT] # 225 627 2973 # # AhaIII*: # [TTTAAA] # 385 473 1245 1722 # # AjuI: # [GAANNNNNNNTTGG] # 32 672 3121 # # AluI: # [AGCT] # 6 27 44 137 157 172 # 176 273 300 395 472 564 # 1102 # # AlwNI: # [CAGNNNCTG] # 1581 2244 # # ApaI: # [GGGCCC] # 260 3565 # # ApoI: # [RAATTY] # 27 58 62 72 74 79 # 95 109 143 180 222 272 # 359 372 458 606 637 # # AsuI*: # [GGNCC] # 1 66 99 118 129 157 # 159 236 292 1261 1307 # # AvaII: # [GGWCC] # 157 195 218 236 292 1307 # 1420 # # BaeI: # [ACNNNNGTAYC] # 33 425 3367 # # BbvI: # [GCAGC] # 28 108 432 758 993 1506 # # BbvII*: # [GAAGAC] # 442 1647 1736 # # BccI: # [CCATC] # 44 96 136 148 3401 # # Bce83I*: # [CTTGAG] # 404 679 835 1907 # # BceAI: # [ACGGC] # 149 302 1613 1761 # # BciVI: # [GTATCC] # 281 1155 2389 # # BclI: # [TGATCA] # 577 3248 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 0 0 0 0 34 34 # 34 34 34 307 347 584 # 620 795 1002 # # BetI*: # [WCCGGW] # 460 3365 # # BfiI: # [ACTGGG] # 189 1663 1973 # # BglI: # [GCCNNNNNGGC] # 553 3272 # # BglII: # [AGATCT] # 31 47 135 159 1649 1804 # # BinI*: # [GGATC] # 39 483 1055 1114 1134 # # BisI: # [GCNGC] # 3 83 446 769 993 1531 # # BlsI: # [GCNGC] # 3 83 447 768 993 1531 # # BmgT120I: # [GGNCC] # 1 65 99 118 129 158 # 159 236 292 1261 1307 # # BmtI: # [GCTAGC] # 1022 2803 # # BplI: # [GAGNNNNNCTC] # 0 0 32 457 3336 # # Bpu10I: # [CCTNAGC] # 1703 2122 # # BsaAI: # [YACGTR] # 21 35 228 1672 1869 # # BsaBI: # [GATNNNNATC] # 78 920 1058 1769 # # BsaXI: # [ACNNNNNCTCC] # 30 123 3672 # # BseBI: # [CCWGG] # 131 241 704 998 1751 # # BseGI: # [GGATG] # 80 236 1521 1988 # # BseMII: # [CTCAG] # 13 65 118 130 275 361 # 362 540 1961 # # BseRI: # [GAGGAG] # 332 430 636 704 1723 # # BseSI: # [GKGCMC] # 41 260 3524 # # BsiYI*: # [CCNNNNNNNGG] # 49 212 526 535 753 1750 # # BsmAI: # [GTCTC] # 111 210 241 319 986 1958 # # Bsp120I: # [GGGCCC] # 256 3569 # # Bsp1407I: # [TGTACA] # 165 3660 # # BspCNI: # [CTCAG] # 11 65 120 132 274 361 # 363 538 1961 # # BsrDI: # [GCAATG] # 162 1471 2192 # # BsrI: # [ACTGG] # 63 116 119 189 287 308 # 557 958 1228 # # BssKI: # [CCNGG] # 131 239 704 1000 1751 # # BssNAI: # [GTATAC] # 92 3733 # # BstKTI: # [GATC] # 17 34 39 47 54 65 # 72 122 132 159 236 379 # 459 470 486 1054 # # BtsI: # [GCAGTG] # 185 3640 # # Cac8I: # [GCNNGC] # 154 475 545 958 1693 # # Cfr10I: # [RCCGGY] # 552 3273 # # CfrI: # [YGGCCR] # 1748 2077 # # ClaI: # [ATCGAT] # 41 528 3256 # # Csp6I: # [GTAC] # 81 118 164 222 254 316 # 377 392 411 455 1035 # # CspCI: # [CAANNNNNGTGG] # 35 1814 1976 # # CviAII: # [CATG] # 98 138 203 209 230 349 # 492 614 1492 # # CviJI: # [RGCY] # 6 14 16 19 22 27 # 29 32 44 51 52 58 # 64 65 67 69 82 105 # 105 123 158 165 183 231 # 277 300 395 441 625 # # CviRI*: # [TGCA] # 12 26 80 105 128 167 # 178 180 196 566 696 721 # 770 # # DdeI: # [CTNAG] # 10 44 65 141 143 225 # 259 266 351 371 443 478 # 512 517 # # DpnI: # [GATC] # 17 33 39 47 54 65 # 72 122 133 159 236 379 # 459 470 486 1054 # # DraII: # [RGGNCCY] # 119 256 646 2804 # # DraIII: # [CACNNNGTG] # 1814 2011 # # DsaI*: # [CCRYGG] # 99 3726 # # Eco31I: # [GGTCTC] # 451 3374 # # Eco57I: # [CTGAAG] # 1081 2744 # # Eco57MI: # [CTGRAG] # 244 897 1081 1603 # # EcoP15I: # [CAGCAG] # 57 653 3115 # # EcoRI: # [GAATTC] # 452 3373 # # EcoRII: # [CCWGG] # 131 239 704 1000 1751 # # EcoT22I: # [ATGCAT] # 475 3350 # # Esp3I: # [CGTCTC] # 1097 2728 # # FatI: # [CATG] # 99 138 203 208 230 349 # 492 614 1492 # # FauI: # [CCCGC] # 230 3595 # # FnuDII*: # [CGCG] # 1609 2216 # # FokI: # [GGATG] # 100 223 1514 1988 # # GlaI: # [GCGC] # 158 1064 2603 # # GsuI: # [CTGGAG] # 897 1325 1603 # # HaeII: # [RGCGCY] # 156 3669 # # HaeIII: # [GGCC] # 14 65 244 579 1102 1821 # # HgaI: # [GACGC] # 571 1056 2198 # # HgiAI*: # [GWGCWC] # 418 618 2789 # # HgiCI*: # [GGYRCC] # 135 620 3070 # # HgiJII*: # [GRGCYC] # 260 3565 # # HhaI: # [GCGC] # 157 1064 2604 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 7 7 16 25 32 32 # 32 32 32 247 381 526 # 591 607 1258 # # Hin4II*: # [CCTTC] # 31 70 151 208 266 389 # 1173 1537 # # Hin6I: # [GCGC] # 159 1064 2602 # # HindII: # [GTYRAC] # 1229 2596 # # HindIII: # [AAGCTT] # 956 2869 # # HinfI: # [GANTC] # 7 19 23 63 112 120 # 122 129 140 166 195 196 # 213 282 316 365 623 734 # # HpaI: # [GTTAAC] # 1229 2596 # # HpaII: # [CCGG] # 92 461 3272 # # HphI: # [GGTGA] # 12 98 143 1575 1997 # # Hpy166II: # [GTNNAC] # 82 92 125 134 147 378 # 418 490 1959 # # Hpy178III*: # [TCNNGA] # 15 24 28 31 33 33 # 38 67 85 87 107 121 # 179 186 195 197 201 226 # 351 360 390 871 # # Hpy188I: # [TCNGA] # 57 65 80 87 97 109 # 139 154 164 195 265 471 # 483 560 899 # # Hpy99I: # [CGWCG] # 236 1504 2085 # # KpnI: # [GGTACC] # 616 3209 # # Ksp632I*: # [CTCTTC] # 63 647 675 735 1705 # # MaeI: # [CTAG] # 18 35 41 43 78 115 # 165 179 236 356 549 555 # 650 805 # # MaeII: # [ACGT] # 5 21 35 95 205 227 # 298 366 412 708 1453 # # MaeIII: # [GTNAC] # 63 104 182 303 491 762 # 940 980 # # MboI: # [GATC] # 17 31 39 47 54 65 # 72 122 135 159 236 379 # 459 470 486 1054 # # MboII: # [GAAGA] # 3 3 33 47 60 116 # 118 132 161 179 221 232 # 240 258 310 529 580 603 # # MfeI: # [CAATTG] # 413 3412 # # MlyI: # [GAGTC] # 190 350 613 794 924 954 # # MmeI: # [TCCRAC] # 298 620 976 1931 # # MnlI: # [CCTC] # 3 9 29 29 68 93 # 98 119 149 150 190 217 # 221 230 247 313 332 387 # 450 491 # # MseI: # [TTAA] # 12 16 18 18 23 25 # 33 38 38 47 53 57 # 64 69 81 106 108 120 # 171 216 219 312 323 342 # 403 422 491 # # MslI: # [CAYNNNNRTG] # 569 3256 # # MwoI: # [GCNNNNNNNGC] # 35 226 518 3046 # # NcoI: # [CCATGG] # 99 3726 # # NdeI: # [CATATG] # 324 421 1394 1686 # # NheI: # [GCTAGC] # 1018 2807 # # NlaIII: # [CATG] # 95 138 203 212 230 349 # 492 614 1492 # # NlaIV: # [GGNNCC] # 55 66 119 137 193 425 # 1038 1792 # # NmeAIII: # [GCCGAG] # 597 3228 # # PflMI: # [CCANNNNNTGG] # 49 526 535 965 1750 # # PfoI: # [TCCNGGA] # 1000 2825 # # PleI: # [GAGTC] # 190 351 613 794 924 953 # # PmaCI: # [CACGTG] # 263 3562 # # PpuMI: # [RGGWCCY] # 375 3450 # # PsiI: # [TTATAA] # 332 352 1124 2017 # # PsrI: # [GAACNNNNNNTAC] # 32 32 32 864 875 882 # 1108 # # PstI: # [CTGCAG] # 180 768 2877 # # RsaI: # [GTAC] # 81 118 163 222 254 317 # 377 392 411 455 1035 # # SapI: # [GCTCTTC] # 1705 2120 # # SauI*: # [CCTNAGG] # 1444 2381 # # ScrFI: # [CCNGG] # 131 241 704 998 1751 # # SduI: # [GDGCHC] # 41 260 317 418 2789 # # SecI*: # [CCNNGG] # 99 207 211 240 489 497 # 2082 # # SetI: # [ASST] # 5 6 21 24 24 26 # 26 27 29 29 30 32 # 33 35 38 44 44 47 # 56 62 74 80 81 95 # 100 103 107 112 115 121 # 141 157 163 174 176 190 # 208 219 248 248 275 # # SfaNI: # [GCATC] # 38 47 77 177 1536 1950 # # SfeI*: # [CTRYAG] # 176 772 2877 # # SmlI: # [CTYRAG] # 425 643 835 1922 # # SnaBI: # [TACGTA] # 1869 1956 # # SpeI: # [ACTAGT] # 1463 2362 # # SspI: # [AATATT] # 47 440 1195 2143 # # StyI: # [CCWWGG] # 99 211 696 737 2082 # # TaiI: # [ACGT] # 5 21 35 95 205 230 # 298 366 409 708 1453 # # TaqI: # [TCGA] # 27 39 41 76 81 99 # 132 222 247 275 528 819 # 1239 # # TaqII: # [CACCCA] # 232 1068 2525 # # TatI: # [WGTACW] # 81 165 810 2769 # # TfiI: # [GAWTC] # 19 23 63 122 136 140 # 166 195 196 282 801 836 # 846 # # TseI: # [GCWGC] # 3 83 445 770 993 1531 # # TsoI: # [TARCCA] # 71 503 513 796 906 1036 # # Tsp45I: # [GTSAC] # 104 182 940 2599 # # Tsp4CI*: # [ACNGT] # 47 61 206 280 411 557 # 673 1590 # # TspDTI: # [ATGAA] # 23 37 69 110 116 131 # 143 147 234 329 331 346 # 349 564 896 # # TspEI: # [AATT] # 8 10 11 11 13 18 # 20 25 27 27 28 29 # 29 35 37 43 47 55 # 58 59 62 67 72 74 # 76 78 79 79 82 86 # 99 102 133 133 142 178 # 230 235 237 239 292 460 # # TspGWI: # [ACGGA] # 11 189 454 759 1149 1263 # # TspRI: # [CASTGNN] # 185 225 504 739 951 1221 # # TstI: # [CACNNNNNNTCC] # 32 217 3576 # # VspI: # [ATTAAT] # 53 318 695 750 2009 # # XbaI: # [TCTAGA] # 1557 2268 # # XcmI: # [CCANNNNNNNNNTGG] # 1078 2747 # # XhoII: # [RGATCY] # 31 47 135 159 696 1108 # 1649 # # XmnI: # [GAANNNNTTC] # 859 2966 # # ZraI: # [GACGTC] # 1563 2262 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3825 # Reported_sequences: 1 # Reported_hitcount: 833 #---------------------------------------