Restriction Fragments for TEL01L



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The currently selected gene/sequence is :

TEL01L on chromosome I from coordinates 801 to 1.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.16115.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 34 39 + SspI AATATT 36 36 . . 49 52 + SetI ASST 52 48 . . 50 54 + HphI GGTGA 62 61 . . 76 81 + SspI AATATT 78 78 . . 119 122 + CviRI* TGCA 120 120 . . 134 137 + TaiI ACGT 137 133 . . 134 137 + SetI ASST 137 133 . . 134 137 + MaeII ACGT 134 136 . . 143 146 + MseI TTAA 143 145 . . 159 164 + SspI AATATT 161 161 . . 166 170 + BslFI GGGAC 180 184 . . 166 171 + NlaIV GGNNCC 168 168 . . 167 171 + BmgT120I GGNCC 168 169 . . 167 171 + AvaII GGWCC 167 170 . . 167 171 + AsuI* GGNCC 167 170 . . 170 173 + AciI CCGC 170 172 . . 187 192 + EcoRV GATATC 189 189 . . 207 211 - BccI CCATC 201 202 . . 242 247 + NspBII* CMGCKG 244 244 . . 242 245 + AciI CCGC 242 244 . . 245 249 + DdeI CTNAG 245 248 . . 245 249 - BspCNI CTCAG 237 235 . . 245 249 - BseMII CTCAG 236 234 . . 247 250 - MnlI CCTC 240 239 . . 244 250 - Bpu10I CCTNAGC 245 248 . . 244 250 - BbvCI CCTCAGC 245 248 . . 260 263 + CviRI* TGCA 261 261 . . 256 260 - BceAI ACGGC 241 243 . . 262 271 + MslI CAYNNNNRTG 266 266 . . 276 279 + CviRI* TGCA 277 277 . . 284 287 + Csp6I GTAC 284 286 . . 284 287 + RsaI GTAC 285 285 . . 289 293 + TfiI GAWTC 289 292 . . 289 293 + HinfI GANTC 289 292 . . 293 299 + TspRI CASTGNN 299 290 . . 304 308 + BmgT120I GGNCC 305 306 . . 304 308 + AsuI* GGNCC 304 307 . . 305 308 + CviJI RGCY 306 306 . . 305 308 + HaeIII GGCC 306 306 . . 301 305 - BccI CCATC 295 296 . . 307 312 + DsaI* CCRYGG 307 311 . . 307 312 + StyI CCWWGG 307 311 . . 307 312 + NcoI CCATGG 307 311 . . 307 312 + SecI* CCNNGG 307 311 . . 308 311 + FatI CATG 307 311 . . 308 311 + NlaIII CATG 311 307 . . 308 311 + CviAII CATG 308 310 . . 322 326 + TfiI GAWTC 322 325 . . 322 326 + HinfI GANTC 322 325 . . 325 329 + Hpy188I TCNGA 327 326 . . 326 330 + DdeI CTNAG 326 329 . . 326 330 - BspCNI CTCAG 318 316 . . 326 330 - BseMII CTCAG 317 315 . . 347 351 - BccI CCATC 341 342 . . 357 361 + Tsp4CI* ACNGT 359 358 . . 358 363 + SfeI* CTRYAG 358 362 . . 363 367 + SfaNI GCATC 372 376 . . 363 374 + AlfI GCANNNNNNTGC 352 350 386 384 364 373 + MslI CAYNNNNRTG 368 368 . . 364 368 - FokI GGATG 350 354 . . 364 368 - BseGI GGATG 363 361 . . 366 370 - TspGWI ACGGA 356 354 . . 374 379 + SplI* CGTACG 374 378 . . 363 374 - AlfI GCANNNNNNTGC 386 384 352 350 375 378 + Csp6I GTAC 375 377 . . 375 378 + RsaI GTAC 376 376 . . 376 381 + SnaBI TACGTA 378 378 . . 376 381 + BsaAI YACGTR 378 378 . . 377 380 + TaiI ACGT 380 376 . . 377 380 + SetI ASST 380 376 . . 377 380 + MaeII ACGT 377 379 . . 388 393 + Hpy166II GTNNAC 390 390 . . 388 393 + BssNAI GTATAC 390 390 . . 388 393 + AccI GTMKAC 389 391 . . 398 401 - MnlI CCTC 391 390 . . 401 405 + HphI GGTGA 413 412 . . 411 416 + NspI RCATGY 415 411 . . 412 415 + FatI CATG 411 415 . . 412 415 + NlaIII CATG 415 411 . . 412 415 + CviAII CATG 412 414 . . 444 453 + MslI CAYNNNNRTG 448 448 . . 444 449 + BsaAI YACGTR 446 446 . . 444 453 + OliI CACNNNNGTG 448 448 . . 444 449 + PmaCI CACGTG 446 446 . . 445 448 + TaiI ACGT 448 444 . . 445 448 + SetI ASST 448 444 . . 445 448 + MaeII ACGT 445 447 . . 445 450 + AflIII ACRYGT 445 449 . . 462 465 + DpnI GATC 463 463 . . 462 465 + BstKTI GATC 464 462 . . 462 465 + MboI GATC 461 465 . . 494 498 + MboII GAAGA 506 505 . . 499 503 + Tsp4CI* ACNGT 501 500 . . 506 510 + HphI GGTGA 518 517 . . 513 516 + SetI ASST 516 512 . . 517 520 + FatI CATG 516 520 . . 517 520 + NlaIII CATG 520 516 . . 517 520 + CviAII CATG 517 519 . . 523 527 + FokI GGATG 536 540 . . 523 527 + BseGI GGATG 529 527 . . 524 528 - BccI CCATC 518 519 . . 551 556 + BtsI GCAGTG 558 556 . . 552 558 + TspRI CASTGNN 558 549 . . 558 563 - MmeI TCCRAC 539 537 . . 571 574 + TspEI AATT 570 574 . . 576 579 - MnlI CCTC 569 568 . . 580 584 + MaeIII GTNAC 579 584 . . 583 587 + Tsp4CI* ACNGT 585 584 . . 589 593 + HphI GGTGA 601 600 . . 587 592 - TaqII CACCCA 577 575 . . 607 610 - MnlI CCTC 600 599 . . 610 614 + FokI GGATG 623 627 . . 610 614 + BseGI GGATG 616 614 . . 614 618 + FokI GGATG 627 631 . . 614 618 + BseGI GGATG 620 618 . . 611 615 - BccI CCATC 605 606 . . 615 619 - BccI CCATC 609 610 . . 610 621 - TstI CACNNNNNNTCC 634 629 602 597 624 628 + Hpy188I TCNGA 626 625 . . 644 648 + BslFI GGGAC 658 662 . . 652 656 + MaeIII GTNAC 651 656 . . 662 665 - MnlI CCTC 655 654 . . 661 671 - BsaXI ACNNNNNCTCC 683 680 653 650 676 679 - MnlI CCTC 669 668 . . 675 685 - BsaXI ACNNNNNCTCC 697 694 667 664 689 693 - BsmAI GTCTC 683 687 . . 694 697 + SetI ASST 697 693 . . 698 703 + BalI TGGCCA 700 700 . . 698 703 + CfrI YGGCCR 698 702 . . 699 702 + CviJI RGCY 700 700 . . 699 702 + HaeIII GGCC 700 700 . . 701 705 + BssKI CCNGG 700 705 . . 701 705 + BseBI CCWGG 702 703 . . 701 706 + SecI* CCNNGG 701 705 . . 701 705 + ScrFI CCNGG 702 703 . . 701 705 + EcoRII CCWGG 700 705 . . 716 719 + CviJI RGCY 717 717 . . 737 741 + FokI GGATG 750 754 . . 737 741 + BseGI GGATG 743 741 . . 750 755 - TaqII CACCCA 740 738 . . 780 785 - TaqII CACCCA 770 768 . . 788 793 - TaqII CACCCA 778 776 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 389 412 # # AciI: # [CCGC] # 72 170 559 # # AflIII: # [ACRYGT] # 356 445 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 352 415 # # AsuI*: # [GGNCC] # 137 167 497 # # AvaII: # [GGWCC] # 167 634 # # BalI: # [TGGCCA] # 101 700 # # BbvCI: # [CCTCAGC] # 245 556 # # BccI: # [CCATC] # 4 46 87 94 177 192 # 201 # # BceAI: # [ACGGC] # 241 560 # # BmgT120I: # [GGNCC] # 137 168 496 # # Bpu10I: # [CCTNAGC] # 245 556 # # BsaAI: # [YACGTR] # 68 355 378 # # BsaXI: # [ACNNNNNCTCC] # 14 14 16 104 653 # # BseBI: # [CCWGG] # 99 702 # # BseGI: # [GGATG] # 4 58 87 123 166 363 # # BseMII: # [CTCAG] # 81 236 484 # # BslFI: # [GGGAC] # 143 180 478 # # BsmAI: # [GTCTC] # 118 683 # # BspCNI: # [CTCAG] # 81 237 483 # # BssKI: # [CCNGG] # 101 700 # # BssNAI: # [GTATAC] # 390 411 # # BstKTI: # [GATC] # 337 464 # # BtsI: # [GCAGTG] # 243 558 # # CfrI: # [YGGCCR] # 103 698 # # Csp6I: # [GTAC] # 91 284 426 # # CviAII: # [CATG] # 104 105 284 308 # # CviJI: # [RGCY] # 17 84 306 394 # # CviRI*: # [TGCA] # 16 120 141 524 # # DdeI: # [CTNAG] # 81 245 475 # # DpnI: # [GATC] # 338 463 # # DsaI*: # [CCRYGG] # 307 494 # # EcoRII: # [CCWGG] # 101 700 # # EcoRV: # [GATATC] # 189 612 # # FatI: # [CATG] # 104 105 285 307 # # FokI: # [GGATG] # 4 51 87 123 186 350 # # HaeIII: # [GGCC] # 101 306 394 # # HinfI: # [GANTC] # 33 289 479 # # HphI: # [GGTGA] # 62 83 105 200 351 # # Hpy166II: # [GTNNAC] # 390 411 # # Hpy188I: # [TCNGA] # 175 299 327 # # MaeII: # [ACGT] # 68 134 243 356 # # MaeIII: # [GTNAC] # 72 150 579 # # MboI: # [GATC] # 340 461 # # MboII: # [GAAGA] # 295 506 # # MmeI: # [TCCRAC] # 262 539 # # MnlI: # [CCTC] # 14 31 55 132 151 178 # 240 # # MseI: # [TTAA] # 143 658 # # MslI: # [CAYNNNNRTG] # 80 102 266 353 # # NcoI: # [CCATGG] # 307 494 # # NlaIII: # [CATG] # 104 105 281 311 # # NlaIV: # [GGNNCC] # 168 633 # # NspBII*: # [CMGCKG] # 244 557 # # NspI: # [RCATGY] # 386 415 # # OliI: # [CACNNNNGTG] # 353 448 # # PmaCI: # [CACGTG] # 355 446 # # RsaI: # [GTAC] # 91 285 425 # # ScrFI: # [CCNGG] # 99 702 # # SecI*: # [CCNNGG] # 100 307 394 # # SetI: # [ASST] # 52 68 68 85 104 181 # 243 # # SfaNI: # [GCATC] # 372 429 # # SfeI*: # [CTRYAG] # 358 443 # # SnaBI: # [TACGTA] # 378 423 # # SplI*: # [CGTACG] # 374 427 # # SspI: # [AATATT] # 36 42 83 640 # # StyI: # [CCWWGG] # 307 494 # # TaiI: # [ACGT] # 68 137 243 353 # # TaqII: # [CACCCA] # 8 23 30 163 577 # # TfiI: # [GAWTC] # 33 289 479 # # Tsp4CI*: # [ACNGT] # 84 142 216 359 # # TspEI: # [AATT] # 231 570 # # TspGWI: # [ACGGA] # 356 445 # # TspRI: # [CASTGNN] # 243 259 299 # # TstI: # [CACNNNNNNTCC] # 32 167 602 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 801 # Reported_sequences: 1 # Reported_hitcount: 142 #---------------------------------------