Restriction Fragments for RUF22/RUF22



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The currently selected gene/sequence is :

RUF22/RUF22 on chromosome VI from coordinates 199813 to 199299.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26618.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + TspEI AATT 4 8 . . 6 11 + VspI ATTAAT 7 9 . . 7 10 + MseI TTAA 7 9 . . 10 14 + TspDTI ATGAA 25 23 . . 16 19 + TspEI AATT 15 19 . . 34 37 + MnlI CCTC 44 43 . . 46 49 + SetI ASST 49 45 . . 47 53 + SauI* CCTNAGG 48 51 . . 48 52 + DdeI CTNAG 48 51 . . 55 59 + Tsp4CI* ACNGT 57 56 . . 62 65 + MseI TTAA 62 64 . . 65 68 + SetI ASST 68 64 . . 67 72 + Hpy166II GTNNAC 69 69 . . 67 72 + BssNAI GTATAC 69 69 . . 67 72 + AccI GTMKAC 68 70 . . 73 77 + Hpy188I TCNGA 75 74 . . 80 83 + CviRI* TGCA 81 81 . . 83 88 + ApoI RAATTY 83 87 . . 84 87 + TspEI AATT 83 87 . . 112 115 + CviJI RGCY 113 113 . . 114 117 + MaeI CTAG 114 116 . . 120 125 + Hpy166II GTNNAC 122 122 . . 121 126 + TatI WGTACW 121 125 . . 122 125 + Csp6I GTAC 122 124 . . 122 125 + RsaI GTAC 123 123 . . 139 142 + CviRI* TGCA 140 140 . . 138 143 - BsgI GTGCAG 123 121 . . 146 149 + CviJI RGCY 147 147 . . 153 157 - MboII GAAGA 145 144 . . 159 162 + Csp6I GTAC 159 161 . . 159 162 + RsaI GTAC 160 160 . . 196 200 - Hin4II* CCTTC 190 189 . . 211 221 + FalI AAGNNNNNCTT 202 197 234 229 220 225 + AhaIII* TTTAAA 222 222 . . 221 224 + MseI TTAA 221 223 . . 211 221 - FalI AAGNNNNNCTT 234 229 202 197 235 239 + TfiI GAWTC 235 238 . . 235 239 + HinfI GANTC 235 238 . . 248 251 + MaeI CTAG 248 250 . . 251 254 + Csp6I GTAC 251 253 . . 251 254 + RsaI GTAC 252 252 . . 253 256 + SetI ASST 256 252 . . 257 261 + Tsp4CI* ACNGT 259 258 . . 278 282 + BsmAI GTCTC 283 287 . . 284 289 + ApoI RAATTY 284 288 . . 285 288 + TspEI AATT 284 288 . . 293 297 - MboII GAAGA 285 284 . . 312 317 + SspI AATATT 314 314 . . 319 322 + SetI ASST 322 318 . . 320 330 + BsiYI* CCNNNNNNNGG 326 323 . . 326 331 - GsaI CCCAGC 330 326 . . 326 331 - BseYI CCCAGC 326 330 . . 345 349 + Tsp45I GTSAC 344 349 . . 345 349 + MaeIII GTNAC 344 349 . . 350 355 + Hpy178III* TCNNGA 351 353 . . 353 358 + XhoII RGATCY 353 357 . . 353 358 + BglII AGATCT 353 357 . . 349 354 - Eco57MI CTGRAG 334 332 . . 354 357 + DpnI GATC 355 355 . . 354 357 + BstKTI GATC 356 354 . . 349 354 - GsuI CTGGAG 334 332 . . 354 357 + MboI GATC 353 357 . . 357 360 + MaeI CTAG 357 359 . . 364 369 + AhaIII* TTTAAA 366 366 . . 365 368 + MseI TTAA 365 367 . . 374 377 + DpnI GATC 375 375 . . 374 377 + BstKTI GATC 376 374 . . 374 377 + MboI GATC 373 377 . . 374 378 - BinI* GGATC 368 369 . . 381 384 + TaiI ACGT 384 380 . . 381 384 + SetI ASST 384 380 . . 381 384 + MaeII ACGT 381 383 . . 383 387 + MaeIII GTNAC 382 387 . . 406 409 + SetI ASST 409 405 . . 406 409 + CviJI RGCY 407 407 . . 406 409 + AluI AGCT 407 407 . . 409 412 + TaqI TCGA 409 411 . . 426 429 + TspEI AATT 425 429 . . 450 454 - HphI GGTGA 442 441 . . 467 472 + TatI WGTACW 467 471 . . 467 472 + Bsp1407I TGTACA 467 471 . . 468 471 + Csp6I GTAC 468 470 . . 468 471 + RsaI GTAC 469 469 . . 473 477 + TspDTI ATGAA 488 486 . . 475 484 + XmnI GAANNNNTTC 479 479 . . 488 492 + BsmAI GTCTC 493 497 . . 504 509 + ApoI RAATTY 504 508 . . 505 508 + TspEI AATT 504 508 . . 508 511 + MseI TTAA 508 510 . . 510 513 + TspEI AATT 509 513 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 68 447 # # AhaIII*: # [TTTAAA] # 144 149 222 # # AluI: # [AGCT] # 108 407 # # ApoI: # [RAATTY] # 11 83 201 220 # # BglII: # [AGATCT] # 162 353 # # BinI*: # [GGATC] # 147 368 # # BseYI: # [CCCAGC] # 189 326 # # BsgI: # [GTGCAG] # 123 392 # # BsiYI*: # [CCNNNNNNNGG] # 189 326 # # BsmAI: # [GTCTC] # 22 210 283 # # Bsp1407I: # [TGTACA] # 48 467 # # BssNAI: # [GTATAC] # 69 446 # # BstKTI: # [GATC] # 20 139 356 # # Csp6I: # [GTAC] # 37 47 92 122 217 # # CviJI: # [RGCY] # 34 108 113 260 # # CviRI*: # [TGCA] # 59 81 375 # # DdeI: # [CTNAG] # 48 467 # # DpnI: # [GATC] # 20 140 355 # # Eco57MI: # [CTGRAG] # 181 334 # # FalI: # [AAGNNNNNCTT] # 0 0 32 202 281 # # GsaI: # [CCCAGC] # 185 330 # # GsuI: # [CTGGAG] # 181 334 # # Hin4II*: # [CCTTC] # 190 325 # # HinfI: # [GANTC] # 235 280 # # HphI: # [GGTGA] # 73 442 # # Hpy166II: # [GTNNAC] # 53 69 393 # # Hpy178III*: # [TCNNGA] # 164 351 # # Hpy188I: # [TCNGA] # 75 440 # # MaeI: # [CTAG] # 109 114 134 158 # # MaeII: # [ACGT] # 134 381 # # MaeIII: # [GTNAC] # 38 133 344 # # MboI: # [GATC] # 20 142 353 # # MboII: # [GAAGA] # 140 145 230 # # MnlI: # [CCTC] # 44 471 # # MseI: # [TTAA] # 7 7 55 143 144 159 # # RsaI: # [GTAC] # 37 46 92 123 217 # # SauI*: # [CCTNAGG] # 48 467 # # SetI: # [ASST] # 19 25 49 62 66 106 # 188 # # SspI: # [AATATT] # 201 314 # # TaiI: # [ACGT] # 131 384 # # TaqI: # [TCGA] # 106 409 # # TatI: # [WGTACW] # 48 121 346 # # TfiI: # [GAWTC] # 235 280 # # Tsp45I: # [GTSAC] # 171 344 # # Tsp4CI*: # [ACNGT] # 57 202 256 # # TspDTI: # [ATGAA] # 25 27 463 # # TspEI: # [AATT] # 4 5 6 11 68 79 # 141 201 # # VspI: # [ATTAAT] # 7 508 # # XhoII: # [RGATCY] # 162 353 # # XmnI: # [GAANNNNTTC] # 36 479 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 515 # Reported_sequences: 1 # Reported_hitcount: 90 #---------------------------------------