Restriction Fragments for RDN58-2/RDN58-2



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RDN58-2/RDN58-2 on chromosome XII from coordinates 464708 to 464551.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.32734.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 13 17 + TspGWI ACGGA 28 26 . . 15 20 + XhoII RGATCY 15 19 . . 15 19 + BinI* GGATC 23 24 . . 16 19 + DpnI GATC 17 17 . . 16 19 + BstKTI GATC 18 16 . . 16 19 + MboI GATC 15 19 . . 31 35 + SfaNI GCATC 40 44 . . 33 38 + ClaI ATCGAT 34 36 . . 34 37 + TaqI TCGA 34 36 . . 37 41 + TspDTI ATGAA 52 50 . . 39 43 + MboII GAAGA 51 50 . . 46 50 + BlsI GCNGC 48 47 . . 46 50 + BisI GCNGC 47 48 . . 46 50 + BbvI GCAGC 58 62 . . 46 50 + TseI GCWGC 46 49 . . 46 57 + AlfI GCANNNNNNTGC 35 33 69 67 46 57 - AlfI GCANNNNNNTGC 69 67 35 33 60 65 + SnaBI TACGTA 62 62 . . 60 65 + BsaAI YACGTR 62 62 . . 61 64 + TaiI ACGT 64 60 . . 61 64 + SetI ASST 64 60 . . 61 64 + MaeII ACGT 61 63 . . 71 74 + TspEI AATT 70 74 . . 74 77 + CviRI* TGCA 75 75 . . 78 83 + EcoRI GAATTC 78 82 . . 78 83 + ApoI RAATTY 78 82 . . 79 82 + TspEI AATT 78 82 . . 82 86 - TspGWI ACGGA 72 70 . . 87 91 + TfiI GAWTC 87 90 . . 87 91 + HinfI GANTC 87 90 . . 93 96 + TaqI TCGA 93 95 . . 95 99 + TfiI GAWTC 95 98 . . 95 99 + HinfI GANTC 95 98 . . 107 117 + MwoI GCNNNNNNNGC 113 110 . . 114 117 + Hin6I GCGC 114 116 . . 114 117 + GlaI GCGC 115 115 . . 114 117 + HhaI GCGC 116 114 . . 110 115 - BsrDI GCAATG 109 107 . . 119 124 + StyI CCWWGG 119 123 . . 119 124 + SecI* CCNNGG 119 123 . . 129 133 + BssKI CCNGG 128 133 . . 129 133 + BseBI CCWGG 130 131 . . 129 134 + SecI* CCNNGG 129 133 . . 129 133 + ScrFI CCNGG 130 131 . . 129 133 + EcoRII CCWGG 128 133 . . 136 141 + NspI RCATGY 140 136 . . 136 141 + Cac8I GCNNGC 138 138 . . 136 141 + SphI GCATGC 140 136 . . 137 140 + FatI CATG 136 140 . . 137 140 + NlaIII CATG 140 136 . . 137 140 + CviAII CATG 137 139 . . 150 154 - HgaI GACGC 139 144 . . #--------------------------------------- # # # AlfI: # [GCANNNNNNTGC] # 0 0 34 35 89 # # ApoI: # [RAATTY] # 78 80 # # BbvI: # [GCAGC] # 58 100 # # BinI*: # [GGATC] # 23 135 # # BisI: # [GCNGC] # 47 111 # # BlsI: # [GCNGC] # 48 110 # # BsaAI: # [YACGTR] # 62 96 # # BseBI: # [CCWGG] # 28 130 # # BsrDI: # [GCAATG] # 49 109 # # BssKI: # [CCNGG] # 30 128 # # BstKTI: # [GATC] # 18 140 # # Cac8I: # [GCNNGC] # 20 138 # # ClaI: # [ATCGAT] # 34 124 # # CviAII: # [CATG] # 21 137 # # CviRI*: # [TGCA] # 75 83 # # DpnI: # [GATC] # 17 141 # # EcoRI: # [GAATTC] # 78 80 # # EcoRII: # [CCWGG] # 30 128 # # FatI: # [CATG] # 22 136 # # GlaI: # [GCGC] # 43 115 # # HgaI: # [GACGC] # 19 139 # # HhaI: # [GCGC] # 42 116 # # Hin6I: # [GCGC] # 44 114 # # HinfI: # [GANTC] # 8 63 87 # # MaeII: # [ACGT] # 61 97 # # MboI: # [GATC] # 15 143 # # MboII: # [GAAGA] # 51 107 # # MwoI: # [GCNNNNNNNGC] # 45 113 # # NlaIII: # [CATG] # 18 140 # # NspI: # [RCATGY] # 18 140 # # ScrFI: # [CCNGG] # 28 130 # # SecI*: # [CCNNGG] # 10 29 119 # # SetI: # [ASST] # 64 94 # # SfaNI: # [GCATC] # 40 118 # # SnaBI: # [TACGTA] # 62 96 # # SphI: # [GCATGC] # 18 140 # # StyI: # [CCWWGG] # 39 119 # # TaiI: # [ACGT] # 64 94 # # TaqI: # [TCGA] # 34 59 65 # # TfiI: # [GAWTC] # 8 63 87 # # TseI: # [GCWGC] # 46 112 # # TspDTI: # [ATGAA] # 52 106 # # TspEI: # [AATT] # 8 70 80 # # TspGWI: # [ACGGA] # 28 44 86 # # XhoII: # [RGATCY] # 15 143 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 158 # Reported_sequences: 1 # Reported_hitcount: 52 #---------------------------------------