Restriction Fragments for FLP1/R0010W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

FLP1/R0010W on 2-micron plasmid from coordinates 252 to 1523.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5701.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 14 + BsiYI* CCNNNNNNNGG 10 7 . . 4 14 + PflMI CCANNNNNTGG 10 7 . . 8 11 + TspEI AATT 7 11 . . 33 36 + SetI ASST 36 32 . . 34 40 + SauI* CCTNAGG 35 38 . . 35 39 + DdeI CTNAG 35 38 . . 38 41 + SetI ASST 41 37 . . 64 67 + SetI ASST 67 63 . . 75 78 + SetI ASST 78 74 . . 76 80 + Hin4II* CCTTC 86 85 . . 81 84 + SetI ASST 84 80 . . 77 82 - Eco57MI CTGRAG 62 60 . . 82 86 + HphI GGTGA 94 93 . . 77 82 - Eco57I CTGAAG 62 60 . . 94 104 + MwoI GCNNNNNNNGC 100 97 . . 94 104 + BstAPI GCANNNNNTGC 100 97 . . 103 107 + BlsI GCNGC 105 104 . . 103 107 + BisI GCNGC 104 105 . . 103 107 + TseI GCWGC 103 106 . . 103 107 - BbvI GCAGC 90 94 . . 104 109 - EcoP15I CAGCAG 76 78 . . 115 118 + SetI ASST 118 114 . . 128 132 + FokI GGATG 141 145 . . 128 132 + BseGI GGATG 134 132 . . 125 130 - MmeI TCCRAC 106 104 . . 143 147 + TspGWI ACGGA 158 156 . . 155 160 + Hpy178III* TCNNGA 156 158 . . 162 165 + CviJI RGCY 163 163 . . 165 174 + MslI CAYNNNNRTG 169 169 . . 170 175 + Hpy178III* TCNNGA 171 173 . . 170 175 + BspHI TCATGA 170 174 . . 171 174 + FatI CATG 170 174 . . 171 174 + NlaIII CATG 174 170 . . 171 174 + CviAII CATG 171 173 . . 169 173 - TspDTI ATGAA 159 157 . . 175 178 + SetI ASST 178 174 . . 175 178 + CviJI RGCY 176 176 . . 175 178 + AluI AGCT 176 176 . . 196 199 + TspEI AATT 195 199 . . 202 206 + DdeI CTNAG 202 205 . . 202 206 - BspCNI CTCAG 194 192 . . 202 206 - BseMII CTCAG 193 191 . . 209 212 + TaqI TCGA 209 211 . . 229 234 - Eco57MI CTGRAG 214 212 . . 229 234 - GsuI CTGGAG 214 212 . . 235 240 + AhaIII* TTTAAA 237 237 . . 231 235 - BsrI ACTGG 231 229 . . 236 239 + MseI TTAA 236 238 . . 246 250 + HgaI GACGC 255 260 . . 261 264 + TspEI AATT 260 264 . . 264 269 + Hpy178III* TCNNGA 265 267 . . 270 273 + CviJI RGCY 271 271 . . 272 275 + MnlI CCTC 282 281 . . 277 280 + MseI TTAA 277 279 . . 284 287 + TspEI AATT 283 287 . . 288 292 + TfiI GAWTC 288 291 . . 288 292 + HinfI GANTC 288 291 . . 301 306 + ApoI RAATTY 301 305 . . 302 305 + TspEI AATT 301 305 . . 309 312 + TspEI AATT 308 312 . . 316 326 + BsiYI* CCNNNNNNNGG 322 319 . . 340 344 + Hpy188I TCNGA 342 341 . . 343 348 + EcoRV GATATC 345 345 . . 348 354 + TspRI CASTGNN 354 345 . . 368 371 + CviRI* TGCA 369 369 . . 371 374 + TspEI AATT 370 374 . . 375 379 + Tsp4CI* ACNGT 377 376 . . 379 384 + AsuII TTCGAA 380 382 . . 380 383 + TaqI TCGA 380 382 . . 382 386 + TfiI GAWTC 382 385 . . 382 386 + HinfI GANTC 382 385 . . 388 392 + Hpy188I TCNGA 390 389 . . 391 395 + MboII GAAGA 403 402 . . 411 416 + TsoI TARCCA 427 425 . . 412 415 + CviJI RGCY 413 413 . . 416 420 + Tsp4CI* ACNGT 418 417 . . 431 434 + MseI TTAA 431 433 . . 442 446 + DdeI CTNAG 442 445 . . 448 451 - MnlI CCTC 441 440 . . 451 455 + HphI GGTGA 463 462 . . 458 462 + SfaNI GCATC 467 471 . . 468 471 + DpnI GATC 469 469 . . 468 471 + BstKTI GATC 470 468 . . 468 471 + MboI GATC 467 471 . . 471 477 + TspRI CASTGNN 477 468 . . 473 477 + DdeI CTNAG 473 476 . . 473 477 - BspCNI CTCAG 465 463 . . 473 477 - BseMII CTCAG 464 462 . . 486 491 + ApoI RAATTY 486 490 . . 487 490 + TspEI AATT 486 490 . . 498 503 + Hpy166II GTNNAC 500 500 . . 498 503 + BssNAI GTATAC 500 500 . . 498 503 + AccI GTMKAC 499 501 . . 505 508 + TaqI TCGA 505 507 . . 505 510 + Hpy178III* TCNNGA 506 508 . . 536 539 + TspEI AATT 535 539 . . 540 543 + MnlI CCTC 550 549 . . 541 546 + Ksp632I* CTCTTC 547 550 . . 542 546 - MboII GAAGA 534 533 . . 547 550 + MaeI CTAG 547 549 . . 549 552 + SetI ASST 552 548 . . 549 552 + CviJI RGCY 550 550 . . 549 552 + AluI AGCT 550 550 . . 556 560 - TspDTI ATGAA 546 544 . . 561 566 + MfeI CAATTG 561 565 . . 562 565 + TspEI AATT 561 565 . . 569 573 + MboII GAAGA 581 580 . . 572 576 + TfiI GAWTC 572 575 . . 572 576 + HinfI GANTC 572 575 . . 584 587 + MseI TTAA 584 586 . . 589 594 + AclI AACGTT 590 592 . . 590 593 + TaiI ACGT 593 589 . . 590 593 + SetI ASST 593 589 . . 590 593 + MaeII ACGT 590 592 . . 595 605 + Hin4I GAYNNNNNVTC 586 581 618 613 595 598 + DpnI GATC 596 596 . . 595 598 + BstKTI GATC 597 595 . . 595 598 + MboI GATC 594 598 . . 597 601 + Hpy188I TCNGA 599 598 . . 595 599 - BinI* GGATC 589 590 . . 595 605 - Hin4I GAYNNNNNVTC 618 613 586 581 606 613 + SwaI ATTTAAAT 609 609 . . 607 612 + AhaIII* TTTAAA 609 609 . . 608 611 + MseI TTAA 608 610 . . 611 614 + TspEI AATT 610 614 . . 635 645 - Hin4I GAYNNNNNVTC 658 653 626 621 646 652 + TspRI CASTGNN 652 643 . . 645 649 - BsrI ACTGG 645 643 . . 655 659 + Tsp45I GTSAC 654 659 . . 655 659 + MaeIII GTNAC 654 659 . . 661 665 - BsmAI GTCTC 655 659 . . 701 704 + Hin6I GCGC 701 703 . . 701 704 + GlaI GCGC 702 702 . . 701 704 + HhaI GCGC 703 701 . . 713 717 + BinI* GGATC 721 722 . . 714 717 + DpnI GATC 715 715 . . 714 717 + BstKTI GATC 716 714 . . 714 717 + MboI GATC 713 717 . . 715 720 + ClaI ATCGAT 716 718 . . 716 719 + TaqI TCGA 716 718 . . 718 721 + DpnI GATC 719 719 . . 718 721 + BstKTI GATC 720 718 . . 718 721 + MboI GATC 717 721 . . 718 722 - BinI* GGATC 712 713 . . 735 739 + FokI GGATG 748 752 . . 735 739 + BseGI GGATG 741 739 . . 737 741 + TspDTI ATGAA 752 750 . . 739 744 + ApoI RAATTY 739 743 . . 740 743 + TspEI AATT 739 743 . . 747 750 - MnlI CCTC 740 739 . . 750 755 + EcoRI GAATTC 750 754 . . 750 755 + ApoI RAATTY 750 754 . . 751 754 + TspEI AATT 750 754 . . 754 758 + Hpy188I TCNGA 756 755 . . 760 764 - BsrI ACTGG 760 758 . . 782 786 + BmgT120I GGNCC 783 784 . . 782 786 + AvaII GGWCC 782 785 . . 782 786 + AsuI* GGNCC 782 785 . . 784 789 + Cfr10I RCCGGY 784 788 . . 785 788 + HpaII CCGG 785 787 . . 790 793 + TspEI AATT 789 793 . . 793 797 - MboII GAAGA 785 784 . . 818 821 + TspEI AATT 817 821 . . 823 826 + MseI TTAA 823 825 . . 834 838 + DdeI CTNAG 834 837 . . 839 843 + Hpy188I TCNGA 841 840 . . 842 845 + DpnI GATC 843 843 . . 842 845 + BstKTI GATC 844 842 . . 842 845 + MboI GATC 841 845 . . 846 849 + Csp6I GTAC 846 848 . . 846 849 + RsaI GTAC 847 847 . . 854 859 + HindIII AAGCTT 854 858 . . 855 858 + SetI ASST 858 854 . . 855 858 + CviJI RGCY 856 856 . . 855 858 + AluI AGCT 856 856 . . 861 865 + MboII GAAGA 873 872 . . 871 874 + Hin6I GCGC 871 873 . . 871 874 + GlaI GCGC 872 872 . . 871 874 + HhaI GCGC 873 871 . . 901 904 + CviJI RGCY 902 902 . . 901 904 + HaeIII GGCC 902 902 . . 901 905 + BmgT120I GGNCC 902 903 . . 901 905 + AsuI* GGNCC 901 904 . . 920 924 + MboII GAAGA 932 931 . . 920 925 + BbvII* GAAGAC 927 931 . . 934 937 + SetI ASST 937 933 . . 935 938 + MnlI CCTC 945 944 . . 949 953 + TspDTI ATGAA 964 962 . . 953 959 + DraII RGGNCCY 954 957 . . 954 958 + BmgT120I GGNCC 955 956 . . 954 958 + AsuI* GGNCC 954 957 . . 951 955 - Hin4II* CCTTC 945 944 . . 955 958 + CviJI RGCY 956 956 . . 955 958 + HaeIII GGCC 956 956 . . 961 965 + TspGWI ACGGA 976 974 . . 966 971 + Hpy166II GTNNAC 968 968 . . 966 971 + HindII GTYRAC 968 968 . . 985 988 + TspEI AATT 984 988 . . 993 1004 + BcgI CGANNNNNNTGC 982 980 1016 1014 999 1004 + Cac8I GCNNGC 1001 1001 . . 1010 1015 + DsaI* CCRYGG 1010 1014 . . 1010 1015 + SecI* CCNNGG 1010 1014 . . 1010 1020 + BsiYI* CCNNNNNNNGG 1016 1013 . . 1009 1013 - BceAI ACGGC 994 996 . . 1013 1018 + BalI TGGCCA 1015 1015 . . 1013 1018 + CfrI YGGCCR 1013 1017 . . 1014 1017 + CviJI RGCY 1015 1015 . . 1014 1017 + HaeIII GGCC 1015 1015 . . 1016 1020 + BssKI CCNGG 1015 1020 . . 1016 1020 + BseBI CCWGG 1017 1018 . . 1016 1020 + ScrFI CCNGG 1017 1018 . . 1016 1020 + EcoRII CCWGG 1015 1020 . . 1024 1027 + TaiI ACGT 1027 1023 . . 1024 1027 + SetI ASST 1027 1023 . . 1024 1027 + MaeII ACGT 1024 1026 . . 1026 1031 + Hpy166II GTNNAC 1028 1028 . . 1026 1031 + BssNAI GTATAC 1028 1028 . . 1026 1031 + AccI GTMKAC 1027 1029 . . 1035 1039 + Hpy188I TCNGA 1037 1036 . . 1050 1053 + SetI ASST 1053 1049 . . 1053 1058 + BclI TGATCA 1053 1057 . . 1054 1057 + DpnI GATC 1055 1055 . . 1054 1057 + BstKTI GATC 1056 1054 . . 1054 1057 + MboI GATC 1053 1057 . . 1068 1073 + SpeI ACTAGT 1068 1072 . . 1069 1072 + MaeI CTAG 1069 1071 . . 1080 1083 + Csp6I GTAC 1080 1082 . . 1080 1083 + RsaI GTAC 1081 1081 . . 1085 1090 + EcoT22I ATGCAT 1089 1085 . . 1086 1089 + CviRI* TGCA 1087 1087 . . 1088 1093 + NdeI CATATG 1089 1091 . . 1093 1103 + Hin4I GAYNNNNNVTC 1084 1079 1116 1111 1093 1096 + DpnI GATC 1094 1094 . . 1093 1096 + BstKTI GATC 1095 1093 . . 1093 1096 + MboI GATC 1092 1096 . . 1093 1097 - BinI* GGATC 1087 1088 . . 1093 1103 - Hin4I GAYNNNNNVTC 1116 1111 1084 1079 1125 1129 + FokI GGATG 1138 1142 . . 1125 1129 + BseGI GGATG 1131 1129 . . 1122 1126 - Hin4II* CCTTC 1116 1115 . . 1129 1133 - BsmAI GTCTC 1123 1127 . . 1138 1152 + XcmI CCANNNNNNNNNTGG 1145 1144 . . 1139 1144 + MfeI CAATTG 1139 1143 . . 1140 1143 + TspEI AATT 1139 1143 . . 1144 1149 + BseRI GAGGAG 1159 1157 . . 1144 1147 - MnlI CCTC 1137 1136 . . 1152 1156 + BlsI GCNGC 1154 1153 . . 1152 1156 + BisI GCNGC 1153 1154 . . 1152 1156 + BbvI GCAGC 1164 1168 . . 1152 1156 + TseI GCWGC 1152 1155 . . 1166 1169 + SetI ASST 1169 1165 . . 1166 1169 + CviJI RGCY 1167 1167 . . 1166 1169 + AluI AGCT 1167 1167 . . 1181 1186 + Eco57MI CTGRAG 1202 1200 . . 1181 1186 + Eco57I CTGAAG 1202 1200 . . 1183 1195 + BarI GAAGNNNNNNTAC 1175 1170 1207 1202 1183 1187 - Hin4II* CCTTC 1177 1176 . . 1201 1205 + FauI CCCGC 1209 1211 . . 1202 1205 + AciI CCGC 1202 1204 . . 1205 1208 + FatI CATG 1204 1208 . . 1205 1208 + NlaIII CATG 1208 1204 . . 1205 1208 + CviAII CATG 1205 1207 . . 1229 1232 + SetI ASST 1232 1228 . . 1228 1231 - MnlI CCTC 1221 1220 . . 1231 1234 + Csp6I GTAC 1231 1233 . . 1231 1234 + RsaI GTAC 1232 1232 . . 1234 1237 + MaeI CTAG 1234 1236 . . 1241 1244 + SetI ASST 1244 1240 . . 1245 1249 - TspDTI ATGAA 1235 1233 . . 1247 1251 - FokI GGATG 1233 1237 . . 1247 1251 - BseGI GGATG 1246 1244 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 245 499 528 # # AciI: # [CCGC] # 70 1202 # # AclI: # [AACGTT] # 590 682 # # AhaIII*: # [TTTAAA] # 237 372 663 # # AluI: # [AGCT] # 105 176 306 311 374 # # ApoI: # [RAATTY] # 11 185 253 301 522 # # AsuI*: # [GGNCC] # 53 119 318 782 # # AsuII: # [TTCGAA] # 380 892 # # AvaII: # [GGWCC] # 490 782 # # BalI: # [TGGCCA] # 257 1015 # # BarI: # [GAAGNNNNNNTAC] # 32 65 1175 # # BbvI: # [GCAGC] # 90 108 1074 # # BbvII*: # [GAAGAC] # 345 927 # # BceAI: # [ACGGC] # 278 994 # # BcgI: # [CGANNNNNNTGC] # 34 256 982 # # BclI: # [TGATCA] # 219 1053 # # BinI*: # [GGATC] # 9 123 185 366 589 # # BisI: # [GCNGC] # 104 119 1049 # # BlsI: # [GCNGC] # 105 118 1049 # # BmgT120I: # [GGNCC] # 53 119 317 783 # # BseBI: # [CCWGG] # 255 1017 # # BseGI: # [GGATG] # 26 115 134 390 607 # # BseMII: # [CTCAG] # 193 271 808 # # BseRI: # [GAGGAG] # 113 1159 # # BsiYI*: # [CCNNNNNNNGG] # 10 256 312 694 # # BsmAI: # [GTCTC] # 149 468 655 # # BspCNI: # [CTCAG] # 194 271 807 # # BspHI: # [TCATGA] # 170 1102 # # BsrI: # [ACTGG] # 115 231 414 512 # # BssKI: # [CCNGG] # 257 1015 # # BssNAI: # [GTATAC] # 244 500 528 # # BstAPI: # [GCANNNNNTGC] # 100 1172 # # BstKTI: # [GATC] # 4 39 119 124 127 177 # 212 470 # # Cac8I: # [GCNNGC] # 271 1001 # # Cfr10I: # [RCCGGY] # 488 784 # # CfrI: # [YGGCCR] # 259 1013 # # ClaI: # [ATCGAT] # 556 716 # # Csp6I: # [GTAC] # 41 151 234 846 # # CviAII: # [CATG] # 67 171 1034 # # CviJI: # [RGCY] # 13 46 54 59 95 105 # 137 142 152 163 306 # # CviRI*: # [TGCA] # 185 369 718 # # DdeI: # [CTNAG] # 31 35 167 240 361 438 # # DpnI: # [GATC] # 4 39 119 124 127 178 # 212 469 # # DraII: # [RGGNCCY] # 318 954 # # DsaI*: # [CCRYGG] # 262 1010 # # Eco57I: # [CTGAAG] # 62 70 1140 # # Eco57MI: # [CTGRAG] # 62 70 152 988 # # EcoP15I: # [CAGCAG] # 76 1196 # # EcoRI: # [GAATTC] # 522 750 # # EcoRII: # [CCWGG] # 257 1015 # # EcoRV: # [GATATC] # 345 927 # # EcoT22I: # [ATGCAT] # 183 1089 # # FatI: # [CATG] # 68 170 1034 # # FauI: # [CCCGC] # 63 1209 # # FokI: # [GGATG] # 39 95 141 390 607 # # GlaI: # [GCGC] # 170 400 702 # # GsuI: # [CTGGAG] # 214 1058 # # HaeIII: # [GGCC] # 54 59 257 902 # # HgaI: # [GACGC] # 255 1017 # # HhaI: # [GCGC] # 170 399 703 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 8 32 # 32 32 156 426 586 # # Hin4II*: # [CCTTC] # 61 86 95 171 859 # # Hin6I: # [GCGC] # 170 401 701 # # HindII: # [GTYRAC] # 304 968 # # HindIII: # [AAGCTT] # 418 854 # # HinfI: # [GANTC] # 94 190 288 700 # # HpaII: # [CCGG] # 487 785 # # HphI: # [GGTGA] # 94 369 809 # # Hpy166II: # [GTNNAC] # 60 244 468 500 # # Hpy178III*: # [TCNNGA] # 15 94 156 241 766 # # Hpy188I: # [TCNGA] # 48 85 157 196 209 235 # 342 # # Ksp632I*: # [CTCTTC] # 547 725 # # MaeI: # [CTAG] # 38 165 522 547 # # MaeII: # [ACGT] # 248 434 590 # # MaeIII: # [GTNAC] # 618 654 # # MboI: # [GATC] # 4 39 119 124 127 180 # 212 467 # # MboII: # [GAAGA] # 47 59 88 131 204 340 # 403 # # MfeI: # [CAATTG] # 133 561 578 # # MmeI: # [TCCRAC] # 106 1166 # # MnlI: # [CCTC] # 51 84 109 159 190 192 # 205 282 # # MseI: # [TTAA] # 24 41 153 154 215 236 # 449 # # MslI: # [CAYNNNNRTG] # 169 1103 # # MwoI: # [GCNNNNNNNGC] # 100 1172 # # NdeI: # [CATATG] # 183 1089 # # NlaIII: # [CATG] # 64 174 1034 # # PflMI: # [CCANNNNNTGG] # 10 1262 # # RsaI: # [GTAC] # 40 151 234 847 # # SauI*: # [CCTNAGG] # 35 1237 # # ScrFI: # [CCNGG] # 255 1017 # # SecI*: # [CCNNGG] # 262 1010 # # SetI: # [ASST] # 5 6 11 12 26 26 # 28 34 36 41 60 63 # 79 90 116 265 374 # # SfaNI: # [GCATC] # 467 805 # # SpeI: # [ACTAGT] # 204 1068 # # SwaI: # [ATTTAAAT] # 609 663 # # TaiI: # [ACGT] # 245 434 593 # # TaqI: # [TCGA] # 125 171 209 211 556 # # TfiI: # [GAWTC] # 94 190 288 700 # # TseI: # [GCWGC] # 103 120 1049 # # TsoI: # [TARCCA] # 427 845 # # Tsp45I: # [GTSAC] # 618 654 # # Tsp4CI*: # [ACNGT] # 41 377 854 # # TspDTI: # [ATGAA] # 37 159 206 212 271 387 # # TspEI: # [AATT] # 7 7 11 18 23 26 # 28 39 49 49 62 65 # 116 129 133 155 167 188 # # TspGWI: # [ACGGA] # 158 296 818 # # TspRI: # [CASTGNN] # 123 175 354 620 # # XcmI: # [CCANNNNNNNNNTGG] # 127 1145 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1272 # Reported_sequences: 1 # Reported_hitcount: 271 #---------------------------------------