Restriction Fragments for NME1/NME1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

NME1/NME1 on chromosome XIV from coordinates 585587 to 585926.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19661.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + FatI CATG 4 8 . . 5 8 + NlaIII CATG 8 4 . . 5 8 + CviAII CATG 5 7 . . 15 19 + TfiI GAWTC 15 18 . . 15 19 + HinfI GANTC 15 18 . . 20 24 + Tsp45I GTSAC 19 24 . . 20 24 + MaeIII GTNAC 19 24 . . 28 31 + TaqI TCGA 28 30 . . 31 36 + HindIII AAGCTT 31 35 . . 32 35 + SetI ASST 35 31 . . 32 35 + CviJI RGCY 33 33 . . 32 35 + AluI AGCT 33 33 . . 50 55 + ApoI RAATTY 50 54 . . 51 54 + TspEI AATT 50 54 . . 62 66 + DdeI CTNAG 62 65 . . 62 66 + BspCNI CTCAG 75 73 . . 62 66 + BseMII CTCAG 76 74 . . 87 91 + BsmAI GTCTC 92 96 . . 97 100 + TspEI AATT 96 100 . . 106 109 - AciI CCGC 106 108 . . 113 116 + TaiI ACGT 116 112 . . 113 116 + SetI ASST 116 112 . . 113 116 + MaeII ACGT 113 115 . . 124 129 + AhaIII* TTTAAA 126 126 . . 125 128 + MseI TTAA 125 127 . . 129 134 + ApoI RAATTY 129 133 . . 130 133 + TspEI AATT 129 133 . . 133 138 + AhaIII* TTTAAA 135 135 . . 134 137 + MseI TTAA 134 136 . . 137 140 + TspEI AATT 136 140 . . 142 147 + AhaIII* TTTAAA 144 144 . . 143 146 + MseI TTAA 143 145 . . 156 159 + MseI TTAA 156 158 . . 163 167 + FokI GGATG 176 180 . . 163 167 + BseGI GGATG 169 167 . . 161 164 - MnlI CCTC 154 153 . . 164 168 - SfaNI GCATC 154 158 . . 169 174 + StyI CCWWGG 169 173 . . 169 174 + SecI* CCNNGG 169 173 . . 185 190 + Hpy178III* TCNNGA 186 188 . . 190 193 + SetI ASST 193 189 . . 190 196 + SexAI ACCWGGT 190 195 . . 191 195 + BssKI CCNGG 190 195 . . 191 195 + BseBI CCWGG 192 193 . . 191 195 + ScrFI CCNGG 192 193 . . 191 195 + EcoRII CCWGG 190 195 . . 194 199 + KpnI GGTACC 198 194 . . 194 199 + NlaIV GGNNCC 196 196 . . 194 199 + HgiCI* GGYRCC 194 198 . . 194 199 + Acc65I GGTACC 194 198 . . 195 198 + Csp6I GTAC 195 197 . . 195 198 + RsaI GTAC 196 196 . . 197 200 + SetI ASST 200 196 . . 198 201 + MnlI CCTC 208 207 . . 205 208 + CviRI* TGCA 206 206 . . 211 214 + Csp6I GTAC 211 213 . . 211 214 + RsaI GTAC 212 212 . . 213 217 + BsrI ACTGG 218 216 . . 210 220 - BaeI ACNNNNGTAYC 235 230 202 197 223 227 - MboII GAAGA 215 214 . . 229 232 + Csp6I GTAC 229 231 . . 229 232 + RsaI GTAC 230 230 . . 231 235 + BsrI ACTGG 236 234 . . 235 239 + TfiI GAWTC 235 238 . . 235 239 + HinfI GANTC 235 238 . . 238 242 - TspGWI ACGGA 228 226 . . 250 254 + TfiI GAWTC 250 253 . . 250 254 + HinfI GANTC 250 253 . . 260 269 + MslI CAYNNNNRTG 264 264 . . 269 279 + Hin4I GAYNNNNNVTC 260 255 292 287 291 295 + BinI* GGATC 299 300 . . 292 295 + DpnI GATC 293 293 . . 292 295 + BstKTI GATC 294 292 . . 292 295 + MboI GATC 291 295 . . 294 297 + TaqI TCGA 294 296 . . 298 302 + MaeIII GTNAC 297 302 . . 310 313 + SetI ASST 313 309 . . 310 313 + CviJI RGCY 311 311 . . 310 313 + AluI AGCT 311 311 . . 324 328 + BmgT120I GGNCC 325 326 . . 324 328 + AvaII GGWCC 324 327 . . 324 328 + AsuI* GGNCC 324 327 . . 327 332 + DsaI* CCRYGG 327 331 . . 327 332 + StyI CCWWGG 327 331 . . 327 332 + NcoI CCATGG 327 331 . . 327 332 + SecI* CCNNGG 327 331 . . 328 331 + FatI CATG 327 331 . . 328 331 + NlaIII CATG 331 327 . . 328 331 + CviAII CATG 328 330 . . 332 336 + TfiI GAWTC 332 335 . . 332 336 + HinfI GANTC 332 335 . . 335 339 - HphI GGTGA 327 326 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 146 194 # # AciI: # [CCGC] # 106 234 # # AhaIII*: # [TTTAAA] # 9 9 126 196 # # AluI: # [AGCT] # 29 33 278 # # ApoI: # [RAATTY] # 50 79 211 # # AsuI*: # [GGNCC] # 16 324 # # AvaII: # [GGWCC] # 16 324 # # BaeI: # [ACNNNNGTAYC] # 33 105 202 # # BinI*: # [GGATC] # 41 299 # # BmgT120I: # [GGNCC] # 15 325 # # BseBI: # [CCWGG] # 148 192 # # BseGI: # [GGATG] # 169 171 # # BseMII: # [CTCAG] # 76 264 # # BsmAI: # [GTCTC] # 92 248 # # BspCNI: # [CTCAG] # 75 265 # # BsrI: # [ACTGG] # 18 104 218 # # BssKI: # [CCNGG] # 150 190 # # BstKTI: # [GATC] # 46 294 # # Csp6I: # [GTAC] # 16 18 111 195 # # CviAII: # [CATG] # 5 12 323 # # CviJI: # [RGCY] # 29 33 278 # # CviRI*: # [TGCA] # 134 206 # # DdeI: # [CTNAG] # 62 278 # # DpnI: # [GATC] # 47 293 # # DsaI*: # [CCRYGG] # 13 327 # # EcoRII: # [CCWGG] # 150 190 # # FatI: # [CATG] # 4 13 323 # # FokI: # [GGATG] # 164 176 # # HgiCI*: # [GGYRCC] # 146 194 # # Hin4I: # [GAYNNNNNVTC] # 32 48 260 # # HindIII: # [AAGCTT] # 31 309 # # HinfI: # [GANTC] # 8 15 15 82 220 # # HphI: # [GGTGA] # 13 327 # # Hpy178III*: # [TCNNGA] # 154 186 # # KpnI: # [GGTACC] # 142 198 # # MaeII: # [ACGT] # 113 227 # # MaeIII: # [GTNAC] # 19 43 278 # # MboI: # [GATC] # 49 291 # # MboII: # [GAAGA] # 125 215 # # MnlI: # [CCTC] # 54 132 154 # # MseI: # [TTAA] # 9 9 13 125 184 # # MslI: # [CAYNNNNRTG] # 76 264 # # NcoI: # [CCATGG] # 13 327 # # NlaIII: # [CATG] # 8 9 323 # # NlaIV: # [GGNNCC] # 144 196 # # RsaI: # [GTAC] # 16 18 110 196 # # ScrFI: # [CCNGG] # 148 192 # # SecI*: # [CCNNGG] # 13 158 169 # # SetI: # [ASST] # 7 27 35 77 81 113 # # SexAI: # [ACCWGGT] # 150 190 # # SfaNI: # [GCATC] # 154 186 # # StyI: # [CCWWGG] # 13 158 169 # # TaiI: # [ACGT] # 116 224 # # TaqI: # [TCGA] # 28 46 266 # # TfiI: # [GAWTC] # 8 15 15 82 220 # # Tsp45I: # [GTSAC] # 19 321 # # TspEI: # [AATT] # 7 33 46 50 204 # # TspGWI: # [ACGGA] # 112 228 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 340 # Reported_sequences: 1 # Reported_hitcount: 92 #---------------------------------------