Restriction Fragments for CEN7/CEN7



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The currently selected gene/sequence is :

CEN7/CEN7 on chromosome VII from coordinates 497038 to 496920.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.23656.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + BsaAI YACGTR 5 5 . . 3 8 + PmaCI CACGTG 5 5 . . 4 7 + TaiI ACGT 7 3 . . 4 7 + SetI ASST 7 3 . . 4 7 + MaeII ACGT 4 6 . . 4 9 + AflIII ACRYGT 4 8 . . 26 31 + ApoI RAATTY 26 30 . . 27 30 + TspEI AATT 26 30 . . 48 52 + MboII GAAGA 60 59 . . 57 60 + TspEI AATT 56 60 . . 85 90 + PsiI TTATAA 87 87 . . 89 92 + TspEI AATT 88 92 . . 103 107 + Hin4II* CCTTC 113 112 . . 106 110 + Hpy188I TCNGA 108 107 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 4 115 # # ApoI: # [RAATTY] # 26 93 # # BsaAI: # [YACGTR] # 5 114 # # Hin4II*: # [CCTTC] # 6 113 # # Hpy188I: # [TCNGA] # 11 108 # # MaeII: # [ACGT] # 4 115 # # MboII: # [GAAGA] # 59 60 # # PmaCI: # [CACGTG] # 5 114 # # PsiI: # [TTATAA] # 32 87 # # SetI: # [ASST] # 7 112 # # TaiI: # [ACGT] # 7 112 # # TspEI: # [AATT] # 26 30 31 32 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 119 # Reported_sequences: 1 # Reported_hitcount: 14 #---------------------------------------