Restriction Fragments for ARS923



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS923 on chromosome IX from coordinates 245756 to 245925.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.8556.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 17 20 + SetI ASST 20 16 . . 18 28 + EcoNI CCTNNNNNAGG 22 23 . . 18 28 + BsiYI* CCNNNNNNNGG 24 21 . . 20 23 + MseI TTAA 20 22 . . 26 29 + SetI ASST 29 25 . . 27 32 + Eco31I GGTCTC 33 37 . . 28 32 + BsmAI GTCTC 33 37 . . 32 38 + TspRI CASTGNN 38 29 . . 68 71 + CviRI* TGCA 69 69 . . 70 73 + FatI CATG 69 73 . . 70 73 + NlaIII CATG 73 69 . . 70 73 + CviAII CATG 70 72 . . 73 77 + BslFI GGGAC 87 91 . . 101 104 + FnuDII* CGCG 102 102 . . 105 110 + PsiI TTATAA 107 107 . . 121 124 + MseI TTAA 121 123 . . 140 144 + TfiI GAWTC 140 143 . . 140 144 + HinfI GANTC 140 143 . . 144 150 + TspRI CASTGNN 150 141 . . #--------------------------------------- # # # BsiYI*: # [CCNNNNNNNGG] # 24 146 # # BslFI: # [GGGAC] # 83 87 # # BsmAI: # [GTCTC] # 33 137 # # CviAII: # [CATG] # 70 100 # # CviRI*: # [TGCA] # 69 101 # # Eco31I: # [GGTCTC] # 33 137 # # EcoNI: # [CCTNNNNNAGG] # 22 148 # # FatI: # [CATG] # 69 101 # # FnuDII*: # [CGCG] # 68 102 # # HinfI: # [GANTC] # 30 140 # # MseI: # [TTAA] # 20 49 101 # # NlaIII: # [CATG] # 73 97 # # PsiI: # [TTATAA] # 63 107 # # SetI: # [ASST] # 9 20 141 # # TfiI: # [GAWTC] # 30 140 # # TspRI: # [CASTGNN] # 20 38 112 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 170 # Reported_sequences: 1 # Reported_hitcount: 19 #---------------------------------------