Restriction Fragments for ARS909



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS909 on chromosome IX from coordinates 105870 to 106053.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30111.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + MaeI CTAG 3 5 . . 22 25 + TspEI AATT 21 25 . . 36 39 + CviJI RGCY 37 37 . . 38 41 + MnlI CCTC 48 47 . . 54 57 + SetI ASST 57 53 . . 55 58 + MnlI CCTC 65 64 . . 59 62 + SetI ASST 62 58 . . 60 64 + Hin4II* CCTTC 70 69 . . 57 61 - HphI GGTGA 49 48 . . 68 71 + SetI ASST 71 67 . . 68 71 + CviJI RGCY 69 69 . . 68 71 + AluI AGCT 69 69 . . 71 74 + TaqI TCGA 71 73 . . 76 79 + TspEI AATT 75 79 . . 78 81 + MseI TTAA 78 80 . . 89 92 + FatI CATG 88 92 . . 89 92 + NlaIII CATG 92 88 . . 89 92 + CviAII CATG 89 91 . . 90 94 + TspDTI ATGAA 105 103 . . 102 107 + Hpy166II GTNNAC 104 104 . . 138 141 + TspEI AATT 137 141 . . 142 147 + AsuII TTCGAA 143 145 . . 143 146 + TaqI TCGA 143 145 . . 147 151 + Tsp4CI* ACNGT 149 148 . . 155 158 + SetI ASST 158 154 . . 155 158 + CviJI RGCY 156 156 . . 155 158 + AluI AGCT 156 156 . . 171 175 - MboII GAAGA 163 162 . . 173 177 - TspDTI ATGAA 163 161 . . #--------------------------------------- # # # AluI: # [AGCT] # 28 69 87 # # AsuII: # [TTCGAA] # 41 143 # # CviAII: # [CATG] # 89 95 # # CviJI: # [RGCY] # 28 32 37 87 # # FatI: # [CATG] # 88 96 # # Hin4II*: # [CCTTC] # 70 114 # # HphI: # [GGTGA] # 49 135 # # Hpy166II: # [GTNNAC] # 80 104 # # MaeI: # [CTAG] # 3 181 # # MboII: # [GAAGA] # 21 163 # # MnlI: # [CCTC] # 17 48 119 # # MseI: # [TTAA] # 78 106 # # NlaIII: # [CATG] # 92 92 # # SetI: # [ASST] # 5 9 26 57 87 # # TaqI: # [TCGA] # 41 71 72 # # Tsp4CI*: # [ACNGT] # 35 149 # # TspDTI: # [ATGAA] # 21 58 105 # # TspEI: # [AATT] # 21 47 54 62 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 184 # Reported_sequences: 1 # Reported_hitcount: 29 #---------------------------------------