Restriction Fragments for ARS818



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS818 on chromosome VIII from coordinates 392180 to 392395.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25045.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 21 26 + Hpy166II GTNNAC 23 23 . . 35 40 + AhaIII* TTTAAA 37 37 . . 36 39 + MseI TTAA 36 38 . . 42 47 + SduI GDGCHC 46 42 . . 42 47 + HgiJII* GRGCYC 46 42 . . 43 46 + CviJI RGCY 44 44 . . 60 63 + FatI CATG 59 63 . . 60 63 + NlaIII CATG 63 59 . . 60 63 + CviAII CATG 60 62 . . 61 65 + TspDTI ATGAA 76 74 . . 67 72 + TatI WGTACW 67 71 . . 67 72 + ScaI AGTACT 69 69 . . 68 71 + Csp6I GTAC 68 70 . . 68 71 + RsaI GTAC 69 69 . . 71 76 + SmlI CTYRAG 71 75 . . 72 77 + Hpy178III* TCNNGA 73 75 . . 71 76 - Bce83I* CTTGAG 56 54 . . 100 104 + TfiI GAWTC 100 103 . . 100 104 + HinfI GANTC 100 103 . . 96 101 - MmeI TCCRAC 77 75 . . 127 132 + AclI AACGTT 128 130 . . 128 131 + TaiI ACGT 131 127 . . 128 131 + SetI ASST 131 127 . . 128 131 + MaeII ACGT 128 130 . . 131 134 + MseI TTAA 131 133 . . 136 139 - MnlI CCTC 129 128 . . 151 154 + MaeI CTAG 151 153 . . 173 178 + AsuII TTCGAA 174 176 . . 174 177 + TaqI TCGA 174 176 . . 179 183 + Tsp4CI* ACNGT 181 180 . . 189 193 + TspDTI ATGAA 204 202 . . 194 197 + SetI ASST 197 193 . . 199 202 + SetI ASST 202 198 . . 202 207 + AsuII TTCGAA 203 205 . . 203 206 + TaqI TCGA 203 205 . . #--------------------------------------- # # # AclI: # [AACGTT] # 88 128 # # AhaIII*: # [TTTAAA] # 37 179 # # AsuII: # [TTCGAA] # 13 29 174 # # Bce83I*: # [CTTGAG] # 56 160 # # Csp6I: # [GTAC] # 68 148 # # CviAII: # [CATG] # 60 156 # # CviJI: # [RGCY] # 44 172 # # FatI: # [CATG] # 59 157 # # HgiJII*: # [GRGCYC] # 46 170 # # HinfI: # [GANTC] # 100 116 # # Hpy166II: # [GTNNAC] # 23 193 # # Hpy178III*: # [TCNNGA] # 73 143 # # MaeI: # [CTAG] # 65 151 # # MaeII: # [ACGT] # 88 128 # # MmeI: # [TCCRAC] # 77 139 # # MnlI: # [CCTC] # 87 129 # # MseI: # [TTAA] # 36 85 95 # # NlaIII: # [CATG] # 63 153 # # RsaI: # [GTAC] # 69 147 # # ScaI: # [AGTACT] # 69 147 # # SduI: # [GDGCHC] # 46 170 # # SetI: # [ASST] # 5 14 66 131 # # SmlI: # [CTYRAG] # 71 145 # # TaiI: # [ACGT] # 85 131 # # TaqI: # [TCGA] # 13 29 174 # # TatI: # [WGTACW] # 67 149 # # TfiI: # [GAWTC] # 100 116 # # Tsp4CI*: # [ACNGT] # 35 181 # # TspDTI: # [ATGAA] # 12 76 128 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 216 # Reported_sequences: 1 # Reported_hitcount: 35 #---------------------------------------