Restriction Fragments for ARS735



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS735 on chromosome VII from coordinates 1002239 to 1002522.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.29876.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + HindIII AAGCTT 2 6 . . 3 6 + SetI ASST 6 2 . . 3 6 + CviJI RGCY 4 4 . . 3 6 + AluI AGCT 4 4 . . 12 15 + MseI TTAA 12 14 . . 15 18 + CviJI RGCY 16 16 . . 17 21 + Hin4II* CCTTC 27 26 . . 21 24 + MaeI CTAG 21 23 . . 33 38 + AflIII ACRYGT 33 37 . . 35 38 + TaiI ACGT 38 34 . . 35 38 + SetI ASST 38 34 . . 35 38 + MaeII ACGT 35 37 . . 54 58 - TspDTI ATGAA 44 42 . . 62 66 + DdeI CTNAG 62 65 . . 81 85 - TspDTI ATGAA 71 69 . . 92 96 + Tsp45I GTSAC 91 96 . . 92 96 + MaeIII GTNAC 91 96 . . 93 98 + Hpy178III* TCNNGA 94 96 . . 108 112 + TfiI GAWTC 108 111 . . 108 112 + HinfI GANTC 108 111 . . 123 126 + CviRI* TGCA 124 124 . . 138 142 - TspDTI ATGAA 128 126 . . 149 152 + MseI TTAA 149 151 . . 156 159 + TspEI AATT 155 159 . . 162 165 + SetI ASST 165 161 . . 164 169 + Hpy166II GTNNAC 166 166 . . 168 171 + SetI ASST 171 167 . . 186 190 + HphI GGTGA 198 197 . . 187 191 + Tsp45I GTSAC 186 191 . . 187 191 + MaeIII GTNAC 186 191 . . 189 193 + HgaI GACGC 198 203 . . 190 195 + MluI ACGCGT 190 194 . . 190 200 + BaeI ACNNNNGTAYC 179 174 212 207 190 195 + AflIII ACRYGT 190 194 . . 191 194 + FnuDII* CGCG 192 192 . . 196 201 + BciVI GTATCC 207 206 . . 200 203 + AciI CCGC 200 202 . . 202 207 + Cac8I GCNNGC 204 204 . . 203 207 + FauI CCCGC 211 213 . . 199 204 - EciI GGCGGA 189 187 . . 204 207 + AciI CCGC 204 206 . . 204 209 + BsrBI CCGCTC 206 206 . . 214 220 + BstEII GGTNACC 214 219 . . 215 219 + Tsp45I GTSAC 214 219 . . 215 219 + MaeIII GTNAC 214 219 . . 217 222 + TaqII CACCCA 233 231 . . 216 220 - HphI GGTGA 208 207 . . 221 224 + FatI CATG 220 224 . . 221 224 + NlaIII CATG 224 220 . . 221 224 + CviAII CATG 221 223 . . 228 231 + MseI TTAA 228 230 . . 230 233 + TspEI AATT 229 233 . . 233 236 + CviRI* TGCA 234 234 . . 242 245 + TspEI AATT 241 245 . . 247 250 + MseI TTAA 247 249 . . 257 260 + SetI ASST 260 256 . . 257 260 + CviJI RGCY 258 258 . . 257 260 + AluI AGCT 258 258 . . 259 262 + MaeI CTAG 259 261 . . 280 283 + SetI ASST 283 279 . . #--------------------------------------- # # # AciI: # [CCGC] # 4 80 200 # # AflIII: # [ACRYGT] # 33 94 157 # # AluI: # [AGCT] # 4 26 254 # # BaeI: # [ACNNNNGTAYC] # 33 72 179 # # BciVI: # [GTATCC] # 77 207 # # BsrBI: # [CCGCTC] # 78 206 # # BstEII: # [GGTNACC] # 70 214 # # Cac8I: # [GCNNGC] # 80 204 # # CviAII: # [CATG] # 63 221 # # CviJI: # [RGCY] # 4 12 26 242 # # CviRI*: # [TGCA] # 50 110 124 # # DdeI: # [CTNAG] # 62 222 # # EciI: # [GGCGGA] # 95 189 # # FatI: # [CATG] # 64 220 # # FauI: # [CCCGC] # 73 211 # # FnuDII*: # [CGCG] # 92 192 # # HgaI: # [GACGC] # 86 198 # # Hin4II*: # [CCTTC] # 27 257 # # HindIII: # [AAGCTT] # 2 282 # # HinfI: # [GANTC] # 108 176 # # HphI: # [GGTGA] # 10 76 198 # # Hpy166II: # [GTNNAC] # 118 166 # # Hpy178III*: # [TCNNGA] # 94 190 # # MaeI: # [CTAG] # 21 25 238 # # MaeII: # [ACGT] # 35 249 # # MaeIII: # [GTNAC] # 28 70 91 95 # # MluI: # [ACGCGT] # 94 190 # # MseI: # [TTAA] # 12 19 37 79 137 # # NlaIII: # [CATG] # 60 224 # # SetI: # [ASST] # 1 6 6 23 32 89 # 127 # # TaiI: # [ACGT] # 38 246 # # TaqII: # [CACCCA] # 51 233 # # TfiI: # [GAWTC] # 108 176 # # Tsp45I: # [GTSAC] # 28 70 91 95 # # TspDTI: # [ATGAA] # 27 44 57 156 # # TspEI: # [AATT] # 12 43 74 155 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 284 # Reported_sequences: 1 # Reported_hitcount: 60 #---------------------------------------