Restriction Fragments for ARS721



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS721 on chromosome VII from coordinates 568490 to 568737.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.17277.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 14 + Ksp632I* CTCTTC 15 18 . . 10 14 - MboII GAAGA 2 1 . . 25 30 + Hpy178III* TCNNGA 26 28 . . 25 30 + BspHI TCATGA 25 29 . . 26 29 + FatI CATG 25 29 . . 26 29 + NlaIII CATG 29 25 . . 26 29 + CviAII CATG 26 28 . . 24 28 - TspDTI ATGAA 14 12 . . 35 40 + PsiI TTATAA 37 37 . . 48 52 - TspDTI ATGAA 38 36 . . 55 60 + Hpy166II GTNNAC 57 57 . . 73 77 + HphI GGTGA 85 84 . . 81 86 + SfeI* CTRYAG 81 85 . . 95 100 + AsuII TTCGAA 96 98 . . 96 99 + TaqI TCGA 96 98 . . 103 107 + Tsp45I GTSAC 102 107 . . 103 107 + MaeIII GTNAC 102 107 . . 114 117 + SetI ASST 117 113 . . 114 117 + CviJI RGCY 115 115 . . 114 117 + AluI AGCT 115 115 . . 126 129 + TaiI ACGT 129 125 . . 126 129 + SetI ASST 129 125 . . 126 129 + MaeII ACGT 126 128 . . 147 151 + DdeI CTNAG 147 150 . . 149 154 + BseRI GAGGAG 164 162 . . 147 151 - BspCNI CTCAG 139 137 . . 147 151 - BseMII CTCAG 138 136 . . 149 152 - MnlI CCTC 142 141 . . 152 155 - MnlI CCTC 145 144 . . 156 159 + CviJI RGCY 157 157 . . 173 177 + TfiI GAWTC 173 176 . . 173 177 + HinfI GANTC 173 176 . . 175 179 - TspDTI ATGAA 165 163 . . 221 226 + VspI ATTAAT 222 224 . . 222 225 + MseI TTAA 222 224 . . 224 229 + SspI AATATT 226 226 . . 234 237 + TspEI AATT 233 237 . . 237 240 + CviRI* TGCA 238 238 . . #--------------------------------------- # # # AluI: # [AGCT] # 115 133 # # AsuII: # [TTCGAA] # 96 152 # # BseMII: # [CTCAG] # 110 138 # # BseRI: # [GAGGAG] # 84 164 # # BspCNI: # [CTCAG] # 109 139 # # BspHI: # [TCATGA] # 25 223 # # CviAII: # [CATG] # 26 222 # # CviJI: # [RGCY] # 42 91 115 # # CviRI*: # [TGCA] # 10 238 # # DdeI: # [CTNAG] # 101 147 # # FatI: # [CATG] # 25 223 # # HinfI: # [GANTC] # 75 173 # # HphI: # [GGTGA] # 85 163 # # Hpy166II: # [GTNNAC] # 57 191 # # Hpy178III*: # [TCNNGA] # 26 222 # # Ksp632I*: # [CTCTTC] # 15 233 # # MaeII: # [ACGT] # 122 126 # # MaeIII: # [GTNAC] # 102 146 # # MboII: # [GAAGA] # 2 246 # # MnlI: # [CCTC] # 3 103 142 # # MseI: # [TTAA] # 26 222 # # NlaIII: # [CATG] # 29 219 # # PsiI: # [TTATAA] # 37 211 # # SetI: # [ASST] # 12 117 119 # # SfeI*: # [CTRYAG] # 81 167 # # SspI: # [AATATT] # 22 226 # # TaiI: # [ACGT] # 119 129 # # TaqI: # [TCGA] # 96 152 # # TfiI: # [GAWTC] # 75 173 # # Tsp45I: # [GTSAC] # 102 146 # # TspDTI: # [ATGAA] # 14 24 83 127 # # TspEI: # [AATT] # 15 233 # # VspI: # [ATTAAT] # 26 222 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 248 # Reported_sequences: 1 # Reported_hitcount: 38 #---------------------------------------