Restriction Fragments for ARS719



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS719 on chromosome VII from coordinates 484930 to 485157.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.21954.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + FatI CATG 4 8 . . 5 8 + NlaIII CATG 8 4 . . 5 8 + CviAII CATG 5 7 . . 6 10 + TspDTI ATGAA 21 19 . . 23 28 + SspI AATATT 25 25 . . 31 34 + CviJI RGCY 32 32 . . 40 44 + FokI GGATG 53 57 . . 40 44 + BseGI GGATG 46 44 . . 47 50 + MseI TTAA 47 49 . . 49 52 + TspEI AATT 48 52 . . 56 66 + FalI AAGNNNNNCTT 47 42 79 74 56 66 - FalI AAGNNNNNCTT 79 74 47 42 67 71 - TspDTI ATGAA 57 55 . . 78 83 + Hpy166II GTNNAC 80 80 . . 91 96 + Hpy166II GTNNAC 93 93 . . 95 99 + BceAI ACGGC 111 113 . . 97 100 + CviJI RGCY 98 98 . . 98 104 + SapI GCTCTTC 105 108 . . 99 104 + Ksp632I* CTCTTC 105 108 . . 100 104 - MboII GAAGA 92 91 . . 109 113 + DdeI CTNAG 109 112 . . 133 138 + Hpy178III* TCNNGA 134 136 . . 155 160 + ClaI ATCGAT 156 158 . . 156 159 + TaqI TCGA 156 158 . . 158 161 + DpnI GATC 159 159 . . 158 161 + BstKTI GATC 160 158 . . 158 161 + MboI GATC 157 161 . . 164 168 + BceAI ACGGC 180 182 . . 216 220 + BmgT120I GGNCC 217 218 . . 216 220 + AvaII GGWCC 216 219 . . 216 220 + AsuI* GGNCC 216 219 . . 220 225 + SfeI* CTRYAG 220 224 . . 224 227 + SetI ASST 227 223 . . 224 227 + CviJI RGCY 225 225 . . 224 227 + AluI AGCT 225 225 . . #--------------------------------------- # # # AluI: # [AGCT] # 3 225 # # AsuI*: # [GGNCC] # 12 216 # # AvaII: # [GGWCC] # 12 216 # # BceAI: # [ACGGC] # 48 69 111 # # BmgT120I: # [GGNCC] # 11 217 # # BseGI: # [GGATG] # 46 182 # # BstKTI: # [GATC] # 68 160 # # ClaI: # [ATCGAT] # 72 156 # # CviAII: # [CATG] # 5 223 # # CviJI: # [RGCY] # 3 32 66 127 # # DdeI: # [CTNAG] # 109 119 # # DpnI: # [GATC] # 69 159 # # FalI: # [AAGNNNNNCTT] # 0 0 32 47 149 # # FatI: # [CATG] # 4 224 # # FokI: # [GGATG] # 53 175 # # Hpy166II: # [GTNNAC] # 13 80 135 # # Hpy178III*: # [TCNNGA] # 94 134 # # Ksp632I*: # [CTCTTC] # 105 123 # # MboI: # [GATC] # 71 157 # # MboII: # [GAAGA] # 92 136 # # MseI: # [TTAA] # 47 181 # # NlaIII: # [CATG] # 8 220 # # SapI: # [GCTCTTC] # 105 123 # # SetI: # [ASST] # 1 227 # # SfeI*: # [CTRYAG] # 8 220 # # SspI: # [AATATT] # 25 203 # # TaqI: # [TCGA] # 72 156 # # TspDTI: # [ATGAA] # 21 36 171 # # TspEI: # [AATT] # 48 180 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 228 # Reported_sequences: 1 # Reported_hitcount: 35 #---------------------------------------