Restriction Fragments for ARS716



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS716 on chromosome VII from coordinates 352760 to 352906.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.7925.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 12 + TfiI GAWTC 8 11 . . 8 12 + HinfI GANTC 8 11 . . 29 33 - MboII GAAGA 21 20 . . 28 33 - BbvII* GAAGAC 21 25 . . 31 35 - TspDTI ATGAA 21 19 . . 39 44 + Hpy178III* TCNNGA 40 42 . . 40 45 + SmlI CTYRAG 40 44 . . 40 45 + XhoI CTCGAG 40 44 . . 40 45 + BmeT110I CYCGRG 41 43 . . 40 45 + AvaI CYCGRG 40 44 . . 41 44 + TaqI TCGA 41 43 . . 41 46 + Hpy178III* TCNNGA 42 44 . . 46 50 + TspDTI ATGAA 61 59 . . 49 54 + ApoI RAATTY 49 53 . . 50 53 + TspEI AATT 49 53 . . 54 58 + DdeI CTNAG 54 57 . . 54 58 + BspCNI CTCAG 67 65 . . 54 58 + BseMII CTCAG 68 66 . . 64 69 + AhaIII* TTTAAA 66 66 . . 65 68 + MseI TTAA 65 67 . . 95 98 + TaiI ACGT 98 94 . . 95 98 + SetI ASST 98 94 . . 95 98 + MaeII ACGT 95 97 . . 99 102 + TspEI AATT 98 102 . . 101 104 + MseI TTAA 101 103 . . 110 114 - MboII GAAGA 102 101 . . 117 122 + AsuII TTCGAA 118 120 . . 118 121 + TaqI TCGA 118 120 . . 122 125 + SetI ASST 125 121 . . 124 129 + Eco57MI CTGRAG 145 143 . . 124 129 + Eco57I CTGAAG 145 143 . . #--------------------------------------- # # # AhaIII*: # [TTTAAA] # 66 81 # # ApoI: # [RAATTY] # 49 98 # # AsuII: # [TTCGAA] # 29 118 # # AvaI: # [CYCGRG] # 40 107 # # BbvII*: # [GAAGAC] # 21 126 # # BmeT110I: # [CYCGRG] # 41 106 # # BseMII: # [CTCAG] # 68 79 # # BspCNI: # [CTCAG] # 67 80 # # DdeI: # [CTNAG] # 54 93 # # Eco57I: # [CTGAAG] # 2 145 # # Eco57MI: # [CTGRAG] # 2 145 # # HinfI: # [GANTC] # 8 139 # # Hpy178III*: # [TCNNGA] # 2 40 105 # # MaeII: # [ACGT] # 52 95 # # MboII: # [GAAGA] # 21 45 81 # # MseI: # [TTAA] # 36 46 65 # # SetI: # [ASST] # 22 27 98 # # SmlI: # [CTYRAG] # 40 107 # # TaiI: # [ACGT] # 49 98 # # TaqI: # [TCGA] # 29 41 77 # # TfiI: # [GAWTC] # 8 139 # # TspDTI: # [ATGAA] # 21 40 86 # # TspEI: # [AATT] # 49 49 49 # # XhoI: # [CTCGAG] # 40 107 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 147 # Reported_sequences: 1 # Reported_hitcount: 31 #---------------------------------------