Restriction Fragments for ARS710



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS710 on chromosome VII from coordinates 203914 to 204155.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26605.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 6 + DpnI GATC 4 4 . . 3 6 + BstKTI GATC 5 3 . . 3 6 + MboI GATC 2 6 . . 5 10 + Hpy178III* TCNNGA 6 8 . . 5 10 + BetI* WCCGGW 5 9 . . 5 10 + BspMII* TCCGGA 5 9 . . 6 9 + HpaII CCGG 6 8 . . 9 13 + MboII GAAGA 21 20 . . 9 14 - Ksp632I* CTCTTC 4 7 . . 24 29 + Hpy166II GTNNAC 26 26 . . 29 34 + NdeI CATATG 30 32 . . 48 53 + ApoI RAATTY 48 52 . . 49 52 + TspEI AATT 48 52 . . 54 57 + CviRI* TGCA 55 55 . . 57 60 + TspEI AATT 56 60 . . 72 75 + MseI TTAA 72 74 . . 91 96 + BsgI GTGCAG 112 110 . . 92 95 + CviRI* TGCA 93 93 . . 119 123 + TspDTI ATGAA 134 132 . . 136 141 + BsrDI GCAATG 143 141 . . 150 154 + TspDTI ATGAA 165 163 . . 152 156 - Hin4II* CCTTC 146 145 . . 164 167 + TspEI AATT 163 167 . . 168 172 + Tsp4CI* ACNGT 170 169 . . 171 176 + Hpy166II GTNNAC 173 173 . . 171 176 + BssNAI GTATAC 173 173 . . 171 176 + AccI GTMKAC 172 174 . . 175 179 + Tsp4CI* ACNGT 177 176 . . 181 184 + CviRI* TGCA 182 182 . . 184 187 + SetI ASST 187 183 . . 182 187 - BspMI ACCTGC 173 177 . . 182 188 - AarI CACCTGC 173 177 . . 194 197 + MnlI CCTC 204 203 . . 197 202 + DsaI* CCRYGG 197 201 . . 197 202 + SecI* CCNNGG 197 201 . . 199 203 + Tsp4CI* ACNGT 201 200 . . 209 212 + TaiI ACGT 212 208 . . 209 212 + SetI ASST 212 208 . . 209 212 + MaeII ACGT 209 211 . . 215 219 + BmgT120I GGNCC 216 217 . . 215 219 + AvaII GGWCC 215 218 . . 215 219 + AsuI* GGNCC 215 218 . . 227 230 + FnuDII* CGCG 228 228 . . 228 231 + Hin6I GCGC 228 230 . . 228 231 + GlaI GCGC 229 229 . . 228 231 + HhaI GCGC 230 228 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 69 173 # # AccI: # [GTMKAC] # 70 172 # # ApoI: # [RAATTY] # 48 194 # # AsuI*: # [GGNCC] # 27 215 # # AvaII: # [GGWCC] # 27 215 # # BetI*: # [WCCGGW] # 5 237 # # BmgT120I: # [GGNCC] # 26 216 # # BsgI: # [GTGCAG] # 112 130 # # BspMI: # [ACCTGC] # 69 173 # # BspMII*: # [TCCGGA] # 5 237 # # BsrDI: # [GCAATG] # 99 143 # # BssNAI: # [GTATAC] # 69 173 # # BstKTI: # [GATC] # 5 237 # # CviRI*: # [TGCA] # 38 55 60 89 # # DpnI: # [GATC] # 4 238 # # DsaI*: # [CCRYGG] # 45 197 # # FnuDII*: # [CGCG] # 14 228 # # GlaI: # [GCGC] # 13 229 # # HhaI: # [GCGC] # 12 230 # # Hin4II*: # [CCTTC] # 96 146 # # Hin6I: # [GCGC] # 14 228 # # HpaII: # [CCGG] # 6 236 # # Hpy166II: # [GTNNAC] # 26 69 147 # # Hpy178III*: # [TCNNGA] # 6 236 # # Ksp632I*: # [CTCTTC] # 4 238 # # MaeII: # [ACGT] # 33 209 # # MboI: # [GATC] # 2 240 # # MboII: # [GAAGA] # 21 221 # # MnlI: # [CCTC] # 38 204 # # MseI: # [TTAA] # 72 170 # # NdeI: # [CATATG] # 30 212 # # SecI*: # [CCNNGG] # 45 197 # # SetI: # [ASST] # 25 30 187 # # TaiI: # [ACGT] # 30 212 # # Tsp4CI*: # [ACNGT] # 7 24 41 170 # # TspDTI: # [ATGAA] # 31 77 134 # # TspEI: # [AATT] # 8 48 79 107 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 242 # Reported_sequences: 1 # Reported_hitcount: 46 #---------------------------------------