Restriction Fragments for ARS709



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS709 on chromosome VII from coordinates 187464 to 187796.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5806.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 13 + DdeI CTNAG 9 12 . . 13 18 + BciVI GTATCC 24 23 . . 16 20 - TspGWI ACGGA 6 4 . . 31 36 + TatI WGTACW 31 35 . . 31 36 + Bsp1407I TGTACA 31 35 . . 32 35 + Csp6I GTAC 32 34 . . 32 35 + RsaI GTAC 33 33 . . 49 54 + NdeI CATATG 50 52 . . 60 65 + VspI ATTAAT 61 63 . . 61 64 + MseI TTAA 61 63 . . 63 66 + TspEI AATT 62 66 . . 67 72 + TatI WGTACW 67 71 . . 68 71 + Csp6I GTAC 68 70 . . 68 71 + RsaI GTAC 69 69 . . 76 79 + SetI ASST 79 75 . . 77 80 + MnlI CCTC 87 86 . . 77 87 + BsiYI* CCNNNNNNNGG 83 80 . . 91 96 + AcyI GRCGYC 92 94 . . 96 99 + MnlI CCTC 106 105 . . 92 96 - HgaI GACGC 81 86 . . 98 103 + Hpy178III* TCNNGA 99 101 . . 98 103 + BspHI TCATGA 98 102 . . 99 102 + FatI CATG 98 102 . . 99 102 + NlaIII CATG 102 98 . . 99 102 + CviAII CATG 99 101 . . 100 104 + TspDTI ATGAA 115 113 . . 114 118 - Hin4II* CCTTC 108 107 . . 118 122 - Hin4II* CCTTC 112 111 . . 145 149 + Tsp45I GTSAC 144 149 . . 145 149 + MaeIII GTNAC 144 149 . . 170 173 + TaiI ACGT 173 169 . . 170 173 + SetI ASST 173 169 . . 170 173 + MaeII ACGT 170 172 . . 175 179 + PleI GAGTC 183 184 . . 175 179 + MlyI GAGTC 184 184 . . 175 179 + HinfI GANTC 175 178 . . 178 182 - MboII GAAGA 170 169 . . 177 182 - BbvII* GAAGAC 170 174 . . 181 185 - MboII GAAGA 173 172 . . 183 187 - TspDTI ATGAA 173 171 . . 195 198 + MnlI CCTC 205 204 . . 199 204 + Hpy166II GTNNAC 201 201 . . 205 208 + TspEI AATT 204 208 . . 214 217 + TspEI AATT 213 217 . . 216 220 - TspDTI ATGAA 206 204 . . 226 230 + TspDTI ATGAA 241 239 . . 238 243 - EcoP15I CAGCAG 210 212 . . 265 269 + Hpy188I TCNGA 267 266 . . 282 286 + DdeI CTNAG 282 285 . . 282 286 + BspCNI CTCAG 295 293 . . 282 286 + BseMII CTCAG 296 294 . . 288 293 + AhaIII* TTTAAA 290 290 . . 289 292 + MseI TTAA 289 291 . . 322 325 + Hin6I GCGC 322 324 . . 322 325 + GlaI GCGC 323 323 . . 322 325 + HhaI GCGC 324 322 . . #--------------------------------------- # # # AcyI: # [GRCGYC] # 92 241 # # AhaIII*: # [TTTAAA] # 43 290 # # BbvII*: # [GAAGAC] # 163 170 # # BciVI: # [GTATCC] # 24 309 # # BseMII: # [CTCAG] # 37 296 # # BsiYI*: # [CCNNNNNNNGG] # 83 250 # # Bsp1407I: # [TGTACA] # 31 302 # # BspCNI: # [CTCAG] # 38 295 # # BspHI: # [TCATGA] # 98 235 # # Csp6I: # [GTAC] # 32 36 265 # # CviAII: # [CATG] # 99 234 # # DdeI: # [CTNAG] # 9 51 273 # # EcoP15I: # [CAGCAG] # 123 210 # # FatI: # [CATG] # 98 235 # # GlaI: # [GCGC] # 10 323 # # HgaI: # [GACGC] # 81 252 # # HhaI: # [GCGC] # 9 324 # # Hin4II*: # [CCTTC] # 4 108 221 # # Hin6I: # [GCGC] # 11 322 # # HinfI: # [GANTC] # 158 175 # # Hpy166II: # [GTNNAC] # 132 201 # # Hpy178III*: # [TCNNGA] # 99 234 # # Hpy188I: # [TCNGA] # 66 267 # # MaeII: # [ACGT] # 163 170 # # MaeIII: # [GTNAC] # 144 189 # # MboII: # [GAAGA] # 3 160 170 # # MlyI: # [GAGTC] # 149 184 # # MnlI: # [CCTC] # 19 87 99 128 # # MseI: # [TTAA] # 44 61 228 # # NdeI: # [CATATG] # 50 283 # # NlaIII: # [CATG] # 102 231 # # PleI: # [GAGTC] # 150 183 # # RsaI: # [GTAC] # 33 36 264 # # SetI: # [ASST] # 79 94 160 # # TaiI: # [ACGT] # 160 173 # # TatI: # [WGTACW] # 31 36 266 # # Tsp45I: # [GTSAC] # 144 189 # # TspDTI: # [ATGAA] # 33 35 58 92 115 # # TspEI: # [AATT] # 9 62 120 142 # # TspGWI: # [ACGGA] # 6 327 # # VspI: # [ATTAAT] # 61 272 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 333 # Reported_sequences: 1 # Reported_hitcount: 56 #---------------------------------------