Restriction Fragments for ARS522



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS522 on chromosome V from coordinates 549566 to 549814.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.1260.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 20 + MaeIII GTNAC 15 20 . . 22 27 + SspI AATATT 24 24 . . 28 33 + VspI ATTAAT 29 31 . . 29 32 + MseI TTAA 29 31 . . 44 48 + TfiI GAWTC 44 47 . . 44 48 + HinfI GANTC 44 47 . . 58 62 + BlsI GCNGC 60 59 . . 58 62 + BisI GCNGC 59 60 . . 58 62 + BbvI GCAGC 70 74 . . 58 62 + TseI GCWGC 58 61 . . 60 63 + SetI ASST 63 59 . . 60 63 + CviJI RGCY 61 61 . . 60 63 + AluI AGCT 61 61 . . 76 81 + SspI AATATT 78 78 . . 80 85 + PsiI TTATAA 82 82 . . 89 92 + TspEI AATT 88 92 . . 98 101 + TspEI AATT 97 101 . . 101 105 + Hpy188I TCNGA 103 102 . . 111 116 + SspI AATATT 113 113 . . 115 118 + MseI TTAA 115 117 . . 118 121 + TspEI AATT 117 121 . . 129 132 + CviRI* TGCA 130 130 . . 131 136 + NdeI CATATG 132 134 . . 146 149 + TspEI AATT 145 149 . . 157 160 + SetI ASST 160 156 . . 186 189 + MnlI CCTC 196 195 . . 217 227 + Hin4I GAYNNNNNVTC 208 203 240 235 222 227 + SacI GAGCTC 226 222 . . 222 227 + Ecl136II GAGCTC 224 224 . . 222 227 + SduI GDGCHC 226 222 . . 222 227 + HgiJII* GRGCYC 226 222 . . 222 227 + HgiAI* GWGCWC 226 222 . . 223 226 + SetI ASST 226 222 . . 223 226 + CviJI RGCY 224 224 . . 223 226 + AluI AGCT 224 224 . . 227 230 + MnlI CCTC 237 236 . . 225 230 - BseRI GAGGAG 216 214 . . 239 244 + SecI* CCNNGG 239 243 . . 236 240 - FokI GGATG 222 226 . . 240 244 + BssKI CCNGG 239 244 . . 236 240 - BseGI GGATG 235 233 . . 240 244 + ScrFI CCNGG 241 242 . . 240 244 + CauII* CCSGG 241 242 . . 241 244 + HpaII CCGG 241 243 . . 244 249 + Hpy166II GTNNAC 246 246 . . #--------------------------------------- # # # AluI: # [AGCT] # 25 61 163 # # BbvI: # [GCAGC] # 70 179 # # BisI: # [GCNGC] # 59 190 # # BlsI: # [GCNGC] # 60 189 # # BseGI: # [GGATG] # 14 235 # # BseRI: # [GAGGAG] # 33 216 # # BssKI: # [CCNGG] # 10 239 # # CauII*: # [CCSGG] # 8 241 # # CviJI: # [RGCY] # 25 61 163 # # CviRI*: # [TGCA] # 119 130 # # Ecl136II: # [GAGCTC] # 25 224 # # FokI: # [GGATG] # 27 222 # # HgiAI*: # [GWGCWC] # 23 226 # # HgiJII*: # [GRGCYC] # 23 226 # # Hin4I: # [GAYNNNNNVTC] # 9 32 208 # # HinfI: # [GANTC] # 44 205 # # HpaII: # [CCGG] # 8 241 # # Hpy166II: # [GTNNAC] # 3 246 # # Hpy188I: # [TCNGA] # 103 146 # # MaeIII: # [GTNAC] # 15 234 # # MnlI: # [CCTC] # 12 41 196 # # MseI: # [TTAA] # 29 86 134 # # NdeI: # [CATATG] # 117 132 # # PsiI: # [TTATAA] # 82 167 # # SacI: # [GAGCTC] # 23 226 # # ScrFI: # [CCNGG] # 8 241 # # SduI: # [GDGCHC] # 23 226 # # SecI*: # [CCNNGG] # 10 239 # # SetI: # [ASST] # 23 63 66 97 # # SspI: # [AATATT] # 24 35 54 136 # # TfiI: # [GAWTC] # 44 205 # # TseI: # [GCWGC] # 58 191 # # TspEI: # [AATT] # 9 20 28 88 104 # # VspI: # [ATTAAT] # 29 220 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 249 # Reported_sequences: 1 # Reported_hitcount: 45 #---------------------------------------