Restriction Fragments for ARS519



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS519 on chromosome V from coordinates 442416 to 442735.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.24817.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 10 - AciI CCGC 7 9 . . 12 15 + MseI TTAA 12 14 . . 14 17 + TspEI AATT 13 17 . . 22 27 + NlaIV GGNNCC 24 24 . . 22 27 + HgiCI* GGYRCC 22 26 . . 20 23 - AciI CCGC 20 22 . . 26 30 + BccI CCATC 34 35 . . 29 34 + Hpy178III* TCNNGA 30 32 . . 43 51 + DraIII CACNNNGTG 48 45 . . 50 55 + CfrI YGGCCR 50 54 . . 51 54 + CviJI RGCY 52 52 . . 51 54 + HaeIII GGCC 52 52 . . 52 56 - BceAI ACGGC 37 39 . . 59 62 + MnlI CCTC 69 68 . . 59 64 + SecI* CCNNGG 59 63 . . 60 65 + BmeT110I CYCGRG 61 63 . . 60 65 + AvaI CYCGRG 60 64 . . 65 69 + FokI GGATG 78 82 . . 65 69 + BseGI GGATG 71 69 . . 66 70 - BccI CCATC 60 61 . . 65 76 - TstI CACNNNNNNTCC 89 84 57 52 77 82 + Hpy178III* TCNNGA 78 80 . . 93 96 + MseI TTAA 93 95 . . 111 114 + TspEI AATT 110 114 . . 113 116 + MseI TTAA 113 115 . . 116 120 + Tsp4CI* ACNGT 118 117 . . 121 131 + BsiYI* CCNNNNNNNGG 127 124 . . 119 123 - BslFI GGGAC 104 108 . . 127 130 - MnlI CCTC 120 119 . . 130 134 + FokI GGATG 143 147 . . 130 134 + BseGI GGATG 136 134 . . 138 142 + MboII GAAGA 150 149 . . 134 139 - MmeI TCCRAC 115 113 . . 138 143 - Ksp632I* CTCTTC 133 136 . . 150 160 + Hin4I GAYNNNNNVTC 141 136 173 168 153 156 + MseI TTAA 153 155 . . 150 160 - Hin4I GAYNNNNNVTC 173 168 141 136 175 180 + AsuII TTCGAA 176 178 . . 176 179 + TaqI TCGA 176 178 . . 198 201 + SetI ASST 201 197 . . 196 200 - Hin4II* CCTTC 190 189 . . 201 204 + CviRI* TGCA 202 202 . . 202 207 + NspI RCATGY 206 202 . . 203 212 + MslI CAYNNNNRTG 207 207 . . 203 206 + FatI CATG 202 206 . . 203 206 + NlaIII CATG 206 202 . . 203 206 + CviAII CATG 203 205 . . 221 224 + FatI CATG 220 224 . . 221 224 + NlaIII CATG 224 220 . . 221 224 + CviAII CATG 221 223 . . 235 238 + SetI ASST 238 234 . . 242 252 + BsiYI* CCNNNNNNNGG 248 245 . . 246 249 + SetI ASST 249 245 . . 247 252 + StyI CCWWGG 247 251 . . 247 252 + SecI* CCNNGG 247 251 . . 274 278 + TauI GCSGC 277 274 . . 274 278 + BlsI GCNGC 276 275 . . 274 278 + BisI GCNGC 275 276 . . 277 281 + BlsI GCNGC 279 278 . . 277 281 + BisI GCNGC 278 279 . . 277 281 + BbvI GCAGC 289 293 . . 277 281 + TseI GCWGC 277 280 . . 274 277 - AciI CCGC 274 276 . . 278 283 + NspBII* CMGCKG 280 280 . . 280 283 - AciI CCGC 280 282 . . 291 295 - Hin4II* CCTTC 285 284 . . 310 314 + DdeI CTNAG 310 313 . . 316 319 + TaiI ACGT 319 315 . . 316 319 + SetI ASST 319 315 . . 316 319 + MaeII ACGT 316 318 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 7 13 40 254 # # AsuII: # [TTCGAA] # 144 176 # # AvaI: # [CYCGRG] # 60 260 # # BbvI: # [GCAGC] # 31 289 # # BccI: # [CCATC] # 26 34 260 # # BceAI: # [ACGGC] # 37 283 # # BisI: # [GCNGC] # 3 42 275 # # BlsI: # [GCNGC] # 3 41 276 # # BmeT110I: # [CYCGRG] # 61 259 # # BseGI: # [GGATG] # 65 71 184 # # BsiYI*: # [CCNNNNNNNGG] # 72 121 127 # # BslFI: # [GGGAC] # 104 216 # # CfrI: # [YGGCCR] # 50 270 # # CviAII: # [CATG] # 18 99 203 # # CviJI: # [RGCY] # 52 268 # # CviRI*: # [TGCA] # 118 202 # # DdeI: # [CTNAG] # 10 310 # # DraIII: # [CACNNNGTG] # 48 272 # # FatI: # [CATG] # 18 100 202 # # FokI: # [GGATG] # 65 78 177 # # HaeIII: # [GGCC] # 52 268 # # HgiCI*: # [GGYRCC] # 22 298 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 141 147 # # Hin4II*: # [CCTTC] # 35 95 190 # # Hpy178III*: # [TCNNGA] # 30 48 242 # # Ksp632I*: # [CTCTTC] # 133 187 # # MaeII: # [ACGT] # 4 316 # # MboII: # [GAAGA] # 150 170 # # MmeI: # [TCCRAC] # 115 205 # # MnlI: # [CCTC] # 51 69 200 # # MseI: # [TTAA] # 12 20 40 81 167 # # MslI: # [CAYNNNNRTG] # 113 207 # # NlaIII: # [CATG] # 18 96 206 # # NlaIV: # [GGNNCC] # 24 296 # # NspBII*: # [CMGCKG] # 40 280 # # NspI: # [RCATGY] # 114 206 # # SecI*: # [CCNNGG] # 59 73 188 # # SetI: # [ASST] # 1 11 37 70 201 # # StyI: # [CCWWGG] # 73 247 # # TaiI: # [ACGT] # 1 319 # # TaqI: # [TCGA] # 144 176 # # TauI: # [GCSGC] # 43 277 # # TseI: # [GCWGC] # 43 277 # # Tsp4CI*: # [ACNGT] # 118 202 # # TspEI: # [AATT] # 13 97 210 # # TstI: # [CACNNNNNNTCC] # 32 57 231 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 320 # Reported_sequences: 1 # Reported_hitcount: 70 #---------------------------------------