Restriction Fragments for ARS517



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS517 on chromosome V from coordinates 406752 to 406953.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.13509.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 10 + Hpy178III* TCNNGA 6 8 . . 10 13 + TspEI AATT 9 13 . . 20 24 + Tsp4CI* ACNGT 22 21 . . 14 24 - BsaXI ACNNNNNCTCC 36 33 6 3 14 25 - TstI CACNNNNNNTCC 38 33 6 1 45 49 + FokI GGATG 58 62 . . 45 49 + BseGI GGATG 51 49 . . 51 56 + HindIII AAGCTT 51 55 . . 52 55 + SetI ASST 55 51 . . 52 55 + CviJI RGCY 53 53 . . 52 55 + AluI AGCT 53 53 . . 106 111 + Hpy166II GTNNAC 108 108 . . 106 111 + BssNAI GTATAC 108 108 . . 106 111 + AccI GTMKAC 107 109 . . 123 126 + TspEI AATT 122 126 . . 129 134 + SmlI CTYRAG 129 133 . . 129 134 + Bce83I* CTTGAG 150 148 . . 138 142 + BslFI GGGAC 152 156 . . 136 139 - AciI CCGC 136 138 . . 134 139 - BsrBI CCGCTC 136 136 . . 136 140 - FauI CCCGC 129 131 . . 157 160 + CviRI* TGCA 158 158 . . 162 165 + TspEI AATT 161 165 . . 171 176 + SmlI CTYRAG 171 175 . . 171 176 + Bce83I* CTTGAG 192 190 . . 178 182 + TspDTI ATGAA 193 191 . . 180 185 + BsmI GAATGC 186 184 . . 182 187 + EcoT22I ATGCAT 186 182 . . 183 186 + CviRI* TGCA 184 184 . . 189 194 + Hpy178III* TCNNGA 190 192 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 95 107 # # AciI: # [CCGC] # 66 136 # # AluI: # [AGCT] # 53 149 # # Bce83I*: # [CTTGAG] # 10 42 150 # # BsaXI: # [ACNNNNNCTCC] # 6 30 166 # # BseGI: # [GGATG] # 51 151 # # BslFI: # [GGGAC] # 50 152 # # BsmI: # [GAATGC] # 16 186 # # BsrBI: # [CCGCTC] # 66 136 # # BssNAI: # [GTATAC] # 94 108 # # CviJI: # [RGCY] # 53 149 # # CviRI*: # [TGCA] # 18 26 158 # # EcoT22I: # [ATGCAT] # 16 186 # # FauI: # [CCCGC] # 73 129 # # FokI: # [GGATG] # 58 144 # # HindIII: # [AAGCTT] # 51 151 # # Hpy166II: # [GTNNAC] # 94 108 # # Hpy178III*: # [TCNNGA] # 6 12 184 # # SetI: # [ASST] # 55 147 # # SmlI: # [CTYRAG] # 31 42 129 # # Tsp4CI*: # [ACNGT] # 22 180 # # TspDTI: # [ATGAA] # 9 193 # # TspEI: # [AATT] # 9 39 41 113 # # TstI: # [CACNNNNNNTCC] # 6 32 164 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 202 # Reported_sequences: 1 # Reported_hitcount: 30 #---------------------------------------