Restriction Fragments for ARS502



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS502 on chromosome V from coordinates 4097 to 4185.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.17007.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + Tsp4CI* ACNGT 5 4 . . 6 10 + MaeIII GTNAC 5 10 . . 14 17 + Hin6I GCGC 14 16 . . 14 17 + GlaI GCGC 15 15 . . 14 17 + HhaI GCGC 16 14 . . 16 27 + AlfI GCANNNNNNTGC 5 3 39 37 25 28 + CviRI* TGCA 26 26 . . 16 27 - AlfI GCANNNNNNTGC 39 37 5 3 33 39 + Bpu10I CCTNAGC 34 37 . . 34 38 + DdeI CTNAG 34 37 . . 40 44 + Tsp4CI* ACNGT 42 41 . . 41 47 + TspRI CASTGNN 47 38 . . 43 47 + Tsp45I GTSAC 42 47 . . 43 47 + MaeIII GTNAC 42 47 . . 56 61 + AflIII ACRYGT 56 60 . . 57 62 + BsaAI YACGTR 59 59 . . 58 61 + TaiI ACGT 61 57 . . 58 61 + SetI ASST 61 57 . . 58 61 + MaeII ACGT 58 60 . . 64 68 + Hpy188I TCNGA 66 65 . . 65 70 + Eco57MI CTGRAG 86 84 . . 65 70 + Eco57I CTGAAG 86 84 . . 72 76 + PleI GAGTC 80 81 . . 72 76 + MlyI GAGTC 81 81 . . 72 76 + HinfI GANTC 72 75 . . 75 79 - TspGWI ACGGA 65 63 . . 83 87 - HgaI GACGC 72 77 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 33 56 # # AlfI: # [GCANNNNNNTGC] # 0 0 5 34 50 # # Bpu10I: # [CCTNAGC] # 34 55 # # BsaAI: # [YACGTR] # 30 59 # # CviRI*: # [TGCA] # 26 63 # # DdeI: # [CTNAG] # 34 55 # # Eco57I: # [CTGAAG] # 3 86 # # Eco57MI: # [CTGRAG] # 3 86 # # GlaI: # [GCGC] # 15 74 # # HgaI: # [GACGC] # 17 72 # # HhaI: # [GCGC] # 16 73 # # Hin6I: # [GCGC] # 14 75 # # HinfI: # [GANTC] # 17 72 # # Hpy188I: # [TCNGA] # 23 66 # # MaeII: # [ACGT] # 31 58 # # MaeIII: # [GTNAC] # 5 37 47 # # MlyI: # [GAGTC] # 8 81 # # PleI: # [GAGTC] # 9 80 # # SetI: # [ASST] # 28 61 # # TaiI: # [ACGT] # 28 61 # # Tsp45I: # [GTSAC] # 42 47 # # Tsp4CI*: # [ACNGT] # 5 37 47 # # TspGWI: # [ACGGA] # 24 65 # # TspRI: # [CASTGNN] # 42 47 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 89 # Reported_sequences: 1 # Reported_hitcount: 27 #---------------------------------------