Restriction Fragments for ARS439



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The currently selected gene/sequence is :

ARS439 on chromosome IV from coordinates 1379641 to 1380100.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28694.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + TaiI ACGT 7 3 . . 4 7 + SetI ASST 7 3 . . 4 7 + MaeII ACGT 4 6 . . 14 19 + AhaIII* TTTAAA 16 16 . . 15 18 + MseI TTAA 15 17 . . 19 22 + SetI ASST 22 18 . . 23 26 + MseI TTAA 23 25 . . 24 29 + TsoI TARCCA 40 38 . . 42 47 + StyI CCWWGG 42 46 . . 42 47 + SecI* CCNNGG 42 46 . . 47 51 + MboII GAAGA 59 58 . . 47 52 - Ksp632I* CTCTTC 42 45 . . 50 53 - MnlI CCTC 43 42 . . 58 62 - Hin4II* CCTTC 52 51 . . 68 71 + MseI TTAA 68 70 . . 70 73 + TspEI AATT 69 73 . . 87 90 + MseI TTAA 87 89 . . 93 96 + MseI TTAA 93 95 . . 116 119 + FatI CATG 115 119 . . 116 119 + NlaIII CATG 119 115 . . 116 119 + CviAII CATG 116 118 . . 114 118 - TspDTI ATGAA 104 102 . . 120 123 + CviRI* TGCA 121 121 . . 154 158 - MboII GAAGA 146 145 . . 174 177 + SetI ASST 177 173 . . 190 194 + Tsp4CI* ACNGT 192 191 . . 212 217 + TatI WGTACW 212 216 . . 213 216 + Csp6I GTAC 213 215 . . 213 216 + RsaI GTAC 214 214 . . 226 231 + PsiI TTATAA 228 228 . . 255 260 + PsiI TTATAA 257 257 . . 252 257 - TsoI TARCCA 242 240 . . 259 264 + SspI AATATT 261 261 . . 275 280 + VspI ATTAAT 276 278 . . 276 279 + MseI TTAA 276 278 . . 292 295 + SetI ASST 295 291 . . 294 298 + DdeI CTNAG 294 297 . . 306 310 - TspGWI ACGGA 296 294 . . 318 321 + TspEI AATT 317 321 . . 326 329 + TspEI AATT 325 329 . . 328 331 + MseI TTAA 328 330 . . 334 338 + Tsp45I GTSAC 333 338 . . 334 338 + MaeIII GTNAC 333 338 . . 382 386 + MaeIII GTNAC 381 386 . . 399 403 + MboII GAAGA 411 410 . . 399 404 + BbvII* GAAGAC 406 410 . . 403 407 + TspGWI ACGGA 418 416 . . 413 416 + DpnI GATC 414 414 . . 413 416 + BstKTI GATC 415 413 . . 413 416 + MboI GATC 412 416 . . 433 438 + BspLU11I* ACATGT 433 437 . . 433 438 + NspI RCATGY 437 433 . . 433 438 + AflIII ACRYGT 433 437 . . 434 437 + FatI CATG 433 437 . . 434 437 + NlaIII CATG 437 433 . . 434 437 + CviAII CATG 434 436 . . 438 443 + Hpy178III* TCNNGA 439 441 . . 438 443 + BspHI TCATGA 438 442 . . 439 442 + FatI CATG 438 442 . . 439 442 + NlaIII CATG 442 438 . . 439 442 + CviAII CATG 439 441 . . 442 445 + DpnI GATC 443 443 . . 442 445 + BstKTI GATC 444 442 . . 442 445 + MboI GATC 441 445 . . 455 459 - MboII GAAGA 447 446 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 27 433 # # AhaIII*: # [TTTAAA] # 16 444 # # BbvII*: # [GAAGAC] # 54 406 # # BspHI: # [TCATGA] # 22 438 # # BspLU11I*: # [ACATGT] # 27 433 # # BstKTI: # [GATC] # 16 29 415 # # Csp6I: # [GTAC] # 213 247 # # CviAII: # [CATG] # 5 21 116 318 # # CviRI*: # [TGCA] # 121 339 # # DdeI: # [CTNAG] # 166 294 # # DpnI: # [GATC] # 17 29 414 # # FatI: # [CATG] # 5 22 115 318 # # Hin4II*: # [CCTTC] # 52 408 # # Hpy178III*: # [TCNNGA] # 21 439 # # Ksp632I*: # [CTCTTC] # 42 418 # # MaeII: # [ACGT] # 4 456 # # MaeIII: # [GTNAC] # 48 79 333 # # MboI: # [GATC] # 19 29 412 # # MboII: # [GAAGA] # 13 36 59 87 265 # # MnlI: # [CCTC] # 43 417 # # MseI: # [TTAA] # 6 8 15 19 45 52 # 132 183 # # NlaIII: # [CATG] # 5 18 119 318 # # NspI: # [RCATGY] # 23 437 # # PsiI: # [TTATAA] # 29 203 228 # # RsaI: # [GTAC] # 214 246 # # SecI*: # [CCNNGG] # 42 418 # # SetI: # [ASST] # 7 15 118 155 165 # # SspI: # [AATATT] # 199 261 # # StyI: # [CCWWGG] # 42 418 # # TaiI: # [ACGT] # 7 453 # # TatI: # [WGTACW] # 212 248 # # TsoI: # [TARCCA] # 40 202 218 # # Tsp45I: # [GTSAC] # 127 333 # # Tsp4CI*: # [ACNGT] # 192 268 # # TspDTI: # [ATGAA] # 104 356 # # TspEI: # [AATT] # 8 69 135 248 # # TspGWI: # [ACGGA] # 42 122 296 # # VspI: # [ATTAAT] # 184 276 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 460 # Reported_sequences: 1 # Reported_hitcount: 65 #---------------------------------------