Restriction Fragments for ARS412



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS412 on chromosome IV from coordinates 316771 to 317230.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5838.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 19 + SetI ASST 19 15 . . 17 20 + MnlI CCTC 27 26 . . 18 23 + Ksp632I* CTCTTC 24 27 . . 19 23 - MboII GAAGA 11 10 . . 47 52 + TatI WGTACW 47 51 . . 47 52 + Bsp1407I TGTACA 47 51 . . 48 51 + Csp6I GTAC 48 50 . . 48 51 + RsaI GTAC 49 49 . . 51 56 + NdeI CATATG 52 54 . . 59 64 + Cac8I GCNNGC 61 61 . . 63 66 + Hin6I GCGC 63 65 . . 63 66 + GlaI GCGC 64 64 . . 63 66 + HhaI GCGC 65 63 . . 64 67 + FnuDII* CGCG 65 65 . . 70 75 + BsaAI YACGTR 72 72 . . 71 74 + TaiI ACGT 74 70 . . 71 74 + SetI ASST 74 70 . . 71 74 + MaeII ACGT 71 73 . . 86 89 + SetI ASST 89 85 . . 86 89 + CviJI RGCY 87 87 . . 86 89 + AluI AGCT 87 87 . . 92 95 + MseI TTAA 92 94 . . 94 97 + TspEI AATT 93 97 . . 97 102 + AhaIII* TTTAAA 99 99 . . 98 101 + MseI TTAA 98 100 . . 100 105 + ApoI RAATTY 100 104 . . 101 104 + TspEI AATT 100 104 . . 122 125 + TspEI AATT 121 125 . . 127 131 + TspGWI ACGGA 142 140 . . 139 143 + DdeI CTNAG 139 142 . . 139 143 - BspCNI CTCAG 131 129 . . 139 143 - BseMII CTCAG 130 128 . . 150 153 + MseI TTAA 150 152 . . 182 187 + EcoP15I CAGCAG 212 214 . . 182 190 + AlwNI CAGNNNCTG 187 184 . . 184 188 + BlsI GCNGC 186 185 . . 184 188 + BisI GCNGC 185 186 . . 184 188 + BbvI GCAGC 196 200 . . 184 188 + TseI GCWGC 184 187 . . 185 190 + PvuII CAGCTG 187 187 . . 185 190 + NspBII* CMGCKG 187 187 . . 186 189 + SetI ASST 189 185 . . 186 189 + CviJI RGCY 187 187 . . 186 189 + AluI AGCT 187 187 . . 191 194 + MseI TTAA 191 193 . . 196 201 + XhoII RGATCY 196 200 . . 196 200 + BinI* GGATC 204 205 . . 197 200 + DpnI GATC 198 198 . . 197 200 + BstKTI GATC 199 197 . . 197 200 + MboI GATC 196 200 . . 214 217 + SetI ASST 217 213 . . 214 217 + CviJI RGCY 215 215 . . 214 217 + AluI AGCT 215 215 . . 221 226 + PsiI TTATAA 223 223 . . 239 243 - MboII GAAGA 231 230 . . 238 243 - BbvII* GAAGAC 231 235 . . 247 250 + MseI TTAA 247 249 . . 249 252 + TspEI AATT 248 252 . . 252 257 + Hpy178III* TCNNGA 253 255 . . 253 258 + SmlI CTYRAG 253 257 . . 253 258 + XhoI CTCGAG 253 257 . . 253 258 + BmeT110I CYCGRG 254 256 . . 253 258 + AvaI CYCGRG 253 257 . . 254 257 + TaqI TCGA 254 256 . . 256 259 - MnlI CCTC 249 248 . . 264 268 - TspDTI ATGAA 254 252 . . 277 280 + MseI TTAA 277 279 . . 282 285 + AciI CCGC 282 284 . . 282 287 + BsrBI CCGCTC 284 284 . . 288 293 + SfeI* CTRYAG 288 292 . . 292 297 + TatI WGTACW 292 296 . . 292 297 + ScaI AGTACT 294 294 . . 293 296 + Csp6I GTAC 293 295 . . 293 296 + RsaI GTAC 294 294 . . 301 315 + XcmI CCANNNNNNNNNTGG 308 307 . . 303 307 + Tsp4CI* ACNGT 305 304 . . 307 312 + PsiI TTATAA 309 309 . . 315 319 + HphI GGTGA 327 326 . . 313 318 - TaqII CACCCA 303 301 . . 331 335 + Hin4II* CCTTC 341 340 . . 335 340 + SecI* CCNNGG 335 339 . . 336 340 + BssKI CCNGG 335 340 . . 336 340 + BseBI CCWGG 337 338 . . 336 340 + ScrFI CCNGG 337 338 . . 336 340 + EcoRII CCWGG 335 340 . . 339 343 + HphI GGTGA 351 350 . . 340 345 + Hpy166II GTNNAC 342 342 . . 345 349 + BssKI CCNGG 344 349 . . 345 349 + BseBI CCWGG 346 347 . . 345 349 + ScrFI CCNGG 346 347 . . 345 349 + EcoRII CCWGG 344 349 . . 353 356 + TaqI TCGA 353 355 . . 355 359 + TfiI GAWTC 355 358 . . 355 359 + HinfI GANTC 355 358 . . 355 364 + XmnI GAANNNNTTC 359 359 . . 367 372 + ApoI RAATTY 367 371 . . 368 371 + TspEI AATT 367 371 . . 375 378 + TspEI AATT 374 378 . . 386 389 + CviRI* TGCA 387 387 . . 404 407 + CviJI RGCY 405 405 . . 403 408 - TsoI TARCCA 393 391 . . 409 413 + Hpy188I TCNGA 411 410 . . 417 421 + HinfI GANTC 417 420 . . 417 421 - PleI GAGTC 411 412 . . 417 421 - MlyI GAGTC 411 411 . . 431 436 + NspI RCATGY 435 431 . . 432 435 + FatI CATG 431 435 . . 432 435 + NlaIII CATG 435 431 . . 432 435 + CviAII CATG 432 434 . . 438 442 - TspDTI ATGAA 428 426 . . #--------------------------------------- # # # AciI: # [CCGC] # 178 282 # # AhaIII*: # [TTTAAA] # 99 361 # # AluI: # [AGCT] # 28 87 100 245 # # AlwNI: # [CAGNNNCTG] # 187 273 # # ApoI: # [RAATTY] # 93 100 267 # # AvaI: # [CYCGRG] # 207 253 # # BbvI: # [GCAGC] # 196 264 # # BbvII*: # [GAAGAC] # 229 231 # # BinI*: # [GGATC] # 204 256 # # BisI: # [GCNGC] # 185 275 # # BlsI: # [GCNGC] # 186 274 # # BmeT110I: # [CYCGRG] # 206 254 # # BsaAI: # [YACGTR] # 72 388 # # BseBI: # [CCWGG] # 9 114 337 # # BseMII: # [CTCAG] # 130 330 # # Bsp1407I: # [TGTACA] # 47 413 # # BspCNI: # [CTCAG] # 131 329 # # BsrBI: # [CCGCTC] # 176 284 # # BssKI: # [CCNGG] # 9 116 335 # # BstKTI: # [GATC] # 199 261 # # Cac8I: # [GCNNGC] # 61 399 # # Csp6I: # [GTAC] # 48 167 245 # # CviAII: # [CATG] # 28 432 # # CviJI: # [RGCY] # 28 55 87 100 190 # # CviRI*: # [TGCA] # 73 387 # # DdeI: # [CTNAG] # 139 321 # # DpnI: # [GATC] # 198 262 # # EcoP15I: # [CAGCAG] # 212 248 # # EcoRII: # [CCWGG] # 9 116 335 # # FatI: # [CATG] # 29 431 # # FnuDII*: # [CGCG] # 65 395 # # GlaI: # [GCGC] # 64 396 # # HhaI: # [GCGC] # 65 395 # # Hin4II*: # [CCTTC] # 119 341 # # Hin6I: # [GCGC] # 63 397 # # HinfI: # [GANTC] # 43 62 355 # # HphI: # [GGTGA] # 24 109 327 # # Hpy166II: # [GTNNAC] # 118 342 # # Hpy178III*: # [TCNNGA] # 207 253 # # Hpy188I: # [TCNGA] # 49 411 # # Ksp632I*: # [CTCTTC] # 24 436 # # MaeII: # [ACGT] # 71 389 # # MboI: # [GATC] # 196 264 # # MboII: # [GAAGA] # 11 220 229 # # MlyI: # [GAGTC] # 49 411 # # MnlI: # [CCTC] # 27 211 222 # # MseI: # [TTAA] # 6 30 41 52 56 92 # 183 # # NdeI: # [CATATG] # 52 408 # # NlaIII: # [CATG] # 25 435 # # NspBII*: # [CMGCKG] # 187 273 # # NspI: # [RCATGY] # 25 435 # # PleI: # [GAGTC] # 49 411 # # PsiI: # [TTATAA] # 86 151 223 # # PvuII: # [CAGCTG] # 187 273 # # RsaI: # [GTAC] # 49 166 245 # # ScaI: # [AGTACT] # 166 294 # # ScrFI: # [CCNGG] # 9 114 337 # # SecI*: # [CCNNGG] # 125 335 # # SetI: # [ASST] # 15 19 28 55 100 243 # # SfeI*: # [CTRYAG] # 172 288 # # SmlI: # [CTYRAG] # 207 253 # # TaiI: # [ACGT] # 74 386 # # TaqI: # [TCGA] # 99 107 254 # # TaqII: # [CACCCA] # 157 303 # # TatI: # [WGTACW] # 47 168 245 # # TfiI: # [GAWTC] # 105 355 # # TseI: # [GCWGC] # 184 276 # # TsoI: # [TARCCA] # 67 393 # # Tsp4CI*: # [ACNGT] # 155 305 # # TspDTI: # [ATGAA] # 32 174 254 # # TspEI: # [AATT] # 7 7 21 86 93 119 # 127 # # TspGWI: # [ACGGA] # 142 318 # # XcmI: # [CCANNNNNNNNNTGG] # 152 308 # # XhoI: # [CTCGAG] # 207 253 # # XhoII: # [RGATCY] # 196 264 # # XmnI: # [GAANNNNTTC] # 101 359 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 460 # Reported_sequences: 1 # Reported_hitcount: 110 #---------------------------------------