Restriction Fragments for ARS410



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS410 on chromosome IV from coordinates 253791 to 254039.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.20209.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 8 + TspEI AATT 4 8 . . 11 16 + EcoT22I ATGCAT 15 11 . . 12 15 + CviRI* TGCA 13 13 . . 18 22 - MboII GAAGA 10 9 . . 31 35 + Hpy188I TCNGA 33 32 . . 39 42 + TspEI AATT 38 42 . . 44 47 + TaiI ACGT 47 43 . . 44 47 + SetI ASST 47 43 . . 44 47 + MaeII ACGT 44 46 . . 43 48 - BtrI CACGTC 45 45 . . 69 73 + TfiI GAWTC 69 72 . . 69 73 + HinfI GANTC 69 72 . . 87 92 + BetI* WCCGGW 87 91 . . 88 91 + HpaII CCGG 88 90 . . 104 109 + Hpy178III* TCNNGA 105 107 . . 104 109 + BspHI TCATGA 104 108 . . 105 108 + FatI CATG 104 108 . . 105 108 + NlaIII CATG 108 104 . . 105 108 + CviAII CATG 105 107 . . 106 110 + TspDTI ATGAA 121 119 . . 103 107 - TspDTI ATGAA 93 91 . . 119 122 - AciI CCGC 119 121 . . 123 126 + MseI TTAA 123 125 . . 128 131 + FatI CATG 127 131 . . 128 131 + NlaIII CATG 131 127 . . 128 131 + CviAII CATG 128 130 . . 129 133 + TspDTI ATGAA 144 142 . . 131 136 + ApoI RAATTY 131 135 . . 132 135 + TspEI AATT 131 135 . . 155 158 + TspEI AATT 154 158 . . 164 167 + CviRI* TGCA 165 165 . . 162 166 - SfaNI GCATC 152 156 . . 168 172 + MaeIII GTNAC 167 172 . . 184 193 + XmnI GAANNNNTTC 188 188 . . 186 190 + Tsp4CI* ACNGT 188 187 . . 187 195 + AlwNI CAGNNNCTG 192 189 . . 192 196 + Hpy188I TCNGA 194 193 . . 196 200 + BceAI ACGGC 212 214 . . 199 209 + MwoI GCNNNNNNNGC 205 202 . . 201 206 + ApoI RAATTY 201 205 . . 202 205 + TspEI AATT 201 205 . . 207 210 + SetI ASST 210 206 . . 207 210 + CviJI RGCY 208 208 . . 207 210 + AluI AGCT 208 208 . . 214 219 + VspI ATTAAT 215 217 . . 215 218 + MseI TTAA 215 217 . . 217 220 + TspEI AATT 216 220 . . 226 231 + SfeI* CTRYAG 226 230 . . 230 233 + SetI ASST 233 229 . . 230 233 + CviJI RGCY 231 231 . . 230 233 + AluI AGCT 231 231 . . 240 245 + ApoI RAATTY 240 244 . . 241 244 + TspEI AATT 240 244 . . #--------------------------------------- # # # AciI: # [CCGC] # 119 130 # # AluI: # [AGCT] # 18 23 208 # # AlwNI: # [CAGNNNCTG] # 57 192 # # ApoI: # [RAATTY] # 9 39 70 131 # # BceAI: # [ACGGC] # 37 212 # # BetI*: # [WCCGGW] # 87 162 # # BspHI: # [TCATGA] # 104 145 # # BtrI: # [CACGTC] # 45 204 # # CviAII: # [CATG] # 23 105 121 # # CviJI: # [RGCY] # 18 23 208 # # CviRI*: # [TGCA] # 13 84 152 # # EcoT22I: # [ATGCAT] # 15 234 # # FatI: # [CATG] # 23 104 122 # # HinfI: # [GANTC] # 69 180 # # HpaII: # [CCGG] # 88 161 # # Hpy178III*: # [TCNNGA] # 105 144 # # Hpy188I: # [TCNGA] # 33 55 161 # # MaeII: # [ACGT] # 44 205 # # MaeIII: # [GTNAC] # 82 167 # # MboII: # [GAAGA] # 10 239 # # MseI: # [TTAA] # 34 92 123 # # MwoI: # [GCNNNNNNNGC] # 44 205 # # NlaIII: # [CATG] # 23 108 118 # # SetI: # [ASST] # 16 23 47 163 # # SfaNI: # [GCATC] # 97 152 # # SfeI*: # [CTRYAG] # 23 226 # # TaiI: # [ACGT] # 47 202 # # TfiI: # [GAWTC] # 69 180 # # Tsp4CI*: # [ACNGT] # 61 188 # # TspDTI: # [ATGAA] # 23 28 93 105 # # TspEI: # [AATT] # 4 9 15 23 24 34 # 47 93 # # VspI: # [ATTAAT] # 34 215 # # XmnI: # [GAANNNNTTC] # 61 188 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 249 # Reported_sequences: 1 # Reported_hitcount: 53 #---------------------------------------