Restriction Fragments for ARS310/ARS310



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS310/ARS310 on chromosome III from coordinates 166503 to 167348.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.1547.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 10 + BsmAI GTCTC 11 15 . . 28 37 + BsaBI GATNNNNATC 32 32 . . 31 34 + SetI ASST 34 30 . . 33 38 + BciVI GTATCC 44 43 . . 41 44 + TspEI AATT 40 44 . . 42 47 + VspI ATTAAT 43 45 . . 43 46 + MseI TTAA 43 45 . . 50 60 - Hin4I GAYNNNNNVTC 73 68 41 36 68 73 + Hpy166II GTNNAC 70 70 . . 74 77 + TspEI AATT 73 77 . . 79 82 + SetI ASST 82 78 . . 77 81 - HphI GGTGA 69 68 . . 92 97 + SpeI ACTAGT 92 96 . . 93 96 + MaeI CTAG 93 95 . . 97 102 + MmeI TCCRAC 122 120 . . 98 108 + BsiYI* CCNNNNNNNGG 104 101 . . 98 108 + PflMI CCANNNNNTGG 104 101 . . 103 108 + SecI* CCNNGG 103 107 . . 104 108 + BssKI CCNGG 103 108 . . 104 108 + BseBI CCWGG 105 106 . . 104 108 + ScrFI CCNGG 105 106 . . 104 108 + EcoRII CCWGG 103 108 . . 112 115 + TspEI AATT 111 115 . . 116 120 + Tsp45I GTSAC 115 120 . . 116 120 + MaeIII GTNAC 115 120 . . 121 124 + MseI TTAA 121 123 . . 131 135 + DdeI CTNAG 131 134 . . 134 137 + SetI ASST 137 133 . . 134 137 + CviJI RGCY 135 135 . . 134 137 + AluI AGCT 135 135 . . 139 143 + BssKI CCNGG 138 143 . . 139 143 + BseBI CCWGG 140 141 . . 139 143 + ScrFI CCNGG 140 141 . . 139 143 + EcoRII CCWGG 138 143 . . 141 144 + SetI ASST 144 140 . . 142 146 + HphI GGTGA 154 153 . . 184 188 + MaeIII GTNAC 183 188 . . 190 194 + DdeI CTNAG 190 193 . . 213 216 + SetI ASST 216 212 . . 218 221 + Csp6I GTAC 218 220 . . 218 221 + RsaI GTAC 219 219 . . 225 230 + SecI* CCNNGG 225 229 . . 227 230 - MnlI CCTC 220 219 . . 243 248 + ApoI RAATTY 243 247 . . 244 247 + TspEI AATT 243 247 . . 265 268 + TspEI AATT 264 268 . . 266 271 + VspI ATTAAT 267 269 . . 267 270 + MseI TTAA 267 269 . . 269 272 + TspEI AATT 268 272 . . 280 283 + SetI ASST 283 279 . . 280 283 + CviJI RGCY 281 281 . . 280 283 + AluI AGCT 281 281 . . 301 306 + ApoI RAATTY 301 305 . . 302 305 + TspEI AATT 301 305 . . 327 331 + Hpy188I TCNGA 329 328 . . 330 333 + DpnI GATC 331 331 . . 330 333 + BstKTI GATC 332 330 . . 330 333 + MboI GATC 329 333 . . 347 350 + TspEI AATT 346 350 . . 366 371 + SspI AATATT 368 368 . . 372 377 + BtgZI GCGATG 387 391 . . 387 392 + TatI WGTACW 387 391 . . 388 391 + Csp6I GTAC 388 390 . . 388 391 + RsaI GTAC 389 389 . . 399 402 + DpnI GATC 400 400 . . 399 402 + BstKTI GATC 401 399 . . 399 402 + MboI GATC 398 402 . . 401 406 + MmeI TCCRAC 426 424 . . 401 405 + Hpy188I TCNGA 403 402 . . 399 403 - BinI* GGATC 393 394 . . 404 412 + Tth111I GACNNNGTC 407 408 . . 410 414 - BslFI GGGAC 395 399 . . 413 417 - BsrI ACTGG 413 411 . . 412 417 - BfiI ACTGGG 407 406 . . 426 430 + FokI GGATG 439 443 . . 426 430 + BseGI GGATG 432 430 . . 428 432 + TspDTI ATGAA 443 441 . . 432 437 + SspI AATATT 434 434 . . 442 447 + TatI WGTACW 442 446 . . 443 446 + Csp6I GTAC 443 445 . . 443 446 + RsaI GTAC 444 444 . . 451 455 + BsrI ACTGG 456 454 . . 461 465 + BsrI ACTGG 466 464 . . 464 468 + FokI GGATG 477 481 . . 464 468 + BseGI GGATG 470 468 . . 465 475 + Hin4I GAYNNNNNVTC 456 451 488 483 474 478 + Hpy188I TCNGA 476 475 . . 465 475 - Hin4I GAYNNNNNVTC 488 483 456 451 477 481 + TfiI GAWTC 477 480 . . 477 481 + HinfI GANTC 477 480 . . 480 484 - MboII GAAGA 472 471 . . 484 488 + BccI CCATC 492 493 . . 488 492 + DdeI CTNAG 488 491 . . 491 494 + SetI ASST 494 490 . . 493 496 + Csp6I GTAC 493 495 . . 493 496 + RsaI GTAC 494 494 . . 504 507 + MseI TTAA 504 506 . . 514 519 + MfeI CAATTG 514 518 . . 515 518 + TspEI AATT 514 518 . . 522 526 + DdeI CTNAG 522 525 . . 528 533 + AhaIII* TTTAAA 530 530 . . 529 532 + MseI TTAA 529 531 . . 536 540 + Tsp4CI* ACNGT 538 537 . . 539 543 + MaeIII GTNAC 538 543 . . 546 549 + FatI CATG 545 549 . . 546 549 + NlaIII CATG 549 545 . . 546 549 + CviAII CATG 546 548 . . 544 548 - TspDTI ATGAA 534 532 . . 553 556 + MseI TTAA 553 555 . . 562 566 + BceAI ACGGC 578 580 . . 568 571 + TspEI AATT 567 571 . . 584 587 + CviRI* TGCA 585 585 . . 594 599 + Hpy166II GTNNAC 596 596 . . 594 599 + HpaI GTTAAC 596 596 . . 594 599 + HindII GTYRAC 596 596 . . 595 598 + MseI TTAA 595 597 . . 600 605 + BspLU11I* ACATGT 600 604 . . 600 605 + NspI RCATGY 604 600 . . 600 605 + AflIII ACRYGT 600 604 . . 601 604 + FatI CATG 600 604 . . 601 604 + NlaIII CATG 604 600 . . 601 604 + CviAII CATG 601 603 . . 610 615 - BsmI GAATGC 610 608 . . 620 623 + FatI CATG 619 623 . . 620 623 + NlaIII CATG 623 619 . . 620 623 + CviAII CATG 620 622 . . 621 625 + TspDTI ATGAA 636 634 . . 624 629 + ApoI RAATTY 624 628 . . 625 628 + TspEI AATT 624 628 . . 630 633 + MseI TTAA 630 632 . . 636 639 + Csp6I GTAC 636 638 . . 636 639 + RsaI GTAC 637 637 . . 639 643 + DdeI CTNAG 639 642 . . 642 645 + SetI ASST 645 641 . . 635 645 - BaeI ACNNNNGTAYC 660 655 627 622 654 658 + SfaNI GCATC 663 667 . . 657 662 + Hpy178III* TCNNGA 658 660 . . 666 669 + MaeI CTAG 666 668 . . 682 685 + TspEI AATT 681 685 . . 684 687 + MseI TTAA 684 686 . . 688 693 + SspI AATATT 690 690 . . 702 705 + MseI TTAA 702 704 . . 710 715 + AcyI GRCGYC 711 713 . . 708 711 - MnlI CCTC 701 700 . . 711 715 - HgaI GACGC 700 705 . . 718 721 + CviJI RGCY 719 719 . . 720 730 + BsiYI* CCNNNNNNNGG 726 723 . . 728 731 + SetI ASST 731 727 . . 738 743 + SspI AATATT 740 740 . . 746 749 + SetI ASST 749 745 . . 747 750 + MnlI CCTC 757 756 . . 761 764 + CviRI* TGCA 762 762 . . 762 766 + BlsI GCNGC 764 763 . . 762 766 + BisI GCNGC 763 764 . . 762 766 + BbvI GCAGC 774 778 . . 762 766 + TseI GCWGC 762 765 . . 769 773 + Tsp4CI* ACNGT 771 770 . . 772 776 + MaeIII GTNAC 771 776 . . 777 780 + SetI ASST 780 776 . . 808 811 + TspEI AATT 807 811 . . 811 816 + TatI WGTACW 811 815 . . 812 815 + Csp6I GTAC 812 814 . . 812 815 + RsaI GTAC 813 813 . . 819 822 + AciI CCGC 819 821 . . #--------------------------------------- # # # AciI: # [CCGC] # 27 819 # # AcyI: # [GRCGYC] # 135 711 # # AflIII: # [ACRYGT] # 246 600 # # AhaIII*: # [TTTAAA] # 316 530 # # AluI: # [AGCT] # 135 146 565 # # ApoI: # [RAATTY] # 58 222 243 323 # # BaeI: # [ACNNNNGTAYC] # 33 186 627 # # BbvI: # [GCAGC] # 72 774 # # BccI: # [CCATC] # 354 492 # # BceAI: # [ACGGC] # 268 578 # # BciVI: # [GTATCC] # 44 802 # # BfiI: # [ACTGGG] # 407 439 # # BinI*: # [GGATC] # 393 453 # # BisI: # [GCNGC] # 83 763 # # BlsI: # [GCNGC] # 82 764 # # BsaBI: # [GATNNNNATC] # 32 814 # # BseBI: # [CCWGG] # 35 105 706 # # BseGI: # [GGATG] # 38 376 432 # # BsiYI*: # [CCNNNNNNNGG] # 104 120 622 # # BslFI: # [GGGAC] # 395 451 # # BsmAI: # [GTCTC] # 11 835 # # BsmI: # [GAATGC] # 236 610 # # BspLU11I*: # [ACATGT] # 246 600 # # BsrI: # [ACTGG] # 10 43 380 413 # # BssKI: # [CCNGG] # 35 103 708 # # BstKTI: # [GATC] # 69 332 445 # # BtgZI: # [GCGATG] # 387 459 # # Csp6I: # [GTAC] # 34 50 55 143 170 176 # 218 # # CviAII: # [CATG] # 19 55 226 546 # # CviJI: # [RGCY] # 127 135 146 438 # # CviRI*: # [TGCA] # 84 177 585 # # DdeI: # [CTNAG] # 34 59 117 131 207 298 # # DpnI: # [GATC] # 69 331 446 # # EcoRII: # [CCWGG] # 35 103 708 # # FatI: # [CATG] # 19 55 227 545 # # FokI: # [GGATG] # 38 369 439 # # HgaI: # [GACGC] # 146 700 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 41 358 # 383 # # HindII: # [GTYRAC] # 250 596 # # HinfI: # [GANTC] # 369 477 # # HpaI: # [GTTAAC] # 250 596 # # HphI: # [GGTGA] # 69 85 692 # # Hpy166II: # [GTNNAC] # 70 250 526 # # Hpy178III*: # [TCNNGA] # 188 658 # # Hpy188I: # [TCNGA] # 73 74 329 370 # # MaeI: # [CTAG] # 93 180 573 # # MaeIII: # [GTNAC] # 68 75 115 233 355 # # MboI: # [GATC] # 69 329 448 # # MboII: # [GAAGA] # 374 472 # # MfeI: # [CAATTG] # 332 514 # # MmeI: # [TCCRAC] # 122 304 420 # # MnlI: # [CCTC] # 56 89 220 481 # # MseI: # [TTAA] # 18 24 25 35 42 43 # 54 78 144 146 237 # # NlaIII: # [CATG] # 19 55 223 549 # # NspI: # [RCATGY] # 242 604 # # PflMI: # [CCANNNNNTGG] # 104 742 # # RsaI: # [GTAC] # 33 50 55 143 170 176 # 219 # # ScrFI: # [CCNGG] # 35 105 706 # # SecI*: # [CCNNGG] # 103 122 621 # # SetI: # [ASST] # 7 18 31 34 48 55 # 66 67 72 86 151 211 # # SfaNI: # [GCATC] # 183 663 # # SpeI: # [ACTAGT] # 92 754 # # SspI: # [AATATT] # 50 66 106 256 368 # # TatI: # [WGTACW] # 35 55 369 387 # # TfiI: # [GAWTC] # 369 477 # # TseI: # [GCWGC] # 84 762 # # Tsp45I: # [GTSAC] # 115 731 # # Tsp4CI*: # [ACNGT] # 75 233 538 # # TspDTI: # [ATGAA] # 91 102 210 443 # # TspEI: # [AATT] # 4 21 33 33 38 39 # 40 45 53 57 57 126 # 132 168 # # Tth111I: # [GACNNNGTC] # 407 439 # # VspI: # [ATTAAT] # 43 224 579 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 846 # Reported_sequences: 1 # Reported_hitcount: 164 #---------------------------------------