Restriction Fragments for ARS222



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS222 on chromosome II from coordinates 704296 to 704566.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14374.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 5 8 + Csp6I GTAC 5 7 . . 5 8 + RsaI GTAC 6 6 . . 10 15 + SduI GDGCHC 14 10 . . 10 15 + HgiAI* GWGCWC 14 10 . . 13 19 + TspRI CASTGNN 19 10 . . 14 18 + Tsp4CI* ACNGT 16 15 . . 17 22 + SduI GDGCHC 21 17 . . 17 22 + BseSI GKGCMC 21 17 . . 25 35 + FalI AAGNNNNNCTT 16 11 48 43 26 29 + SetI ASST 29 25 . . 26 29 + CviJI RGCY 27 27 . . 26 29 + AluI AGCT 27 27 . . 27 33 + EspI* GCTNAGC 28 31 . . 28 32 + DdeI CTNAG 28 31 . . 30 35 + HindIII AAGCTT 30 34 . . 31 34 + SetI ASST 34 30 . . 31 34 + CviJI RGCY 32 32 . . 31 34 + AluI AGCT 32 32 . . 32 42 + MwoI GCNNNNNNNGC 38 35 . . 25 35 - FalI AAGNNNNNCTT 48 43 16 11 61 64 + SetI ASST 64 60 . . 82 86 + Hpy99I CGWCG 86 81 . . 84 87 + TaqI TCGA 84 86 . . 86 89 + DpnI GATC 87 87 . . 86 89 + BstKTI GATC 88 86 . . 86 89 + MboI GATC 85 89 . . 94 97 + SetI ASST 97 93 . . 94 97 + CviJI RGCY 95 95 . . 94 97 + AluI AGCT 95 95 . . 98 103 - BtgZI GCGATG 83 87 . . 112 116 + BmgT120I GGNCC 113 114 . . 112 116 + AvaII GGWCC 112 115 . . 112 116 + AsuI* GGNCC 112 115 . . 114 119 + Hpy178III* TCNNGA 115 117 . . 110 115 - TaqII GACCGA 100 98 . . 122 125 + MseI TTAA 122 124 . . 126 131 + ApoI RAATTY 126 130 . . 127 130 + TspEI AATT 126 130 . . 142 145 + MseI TTAA 142 144 . . 168 171 + TaiI ACGT 171 167 . . 168 171 + SetI ASST 171 167 . . 168 171 + MaeII ACGT 168 170 . . 186 189 + MseI TTAA 186 188 . . 212 215 + TspEI AATT 211 215 . . 218 222 + Tsp4CI* ACNGT 220 219 . . 243 248 + PsiI TTATAA 245 245 . . 249 252 + CviJI RGCY 250 250 . . 263 269 + TspRI CASTGNN 269 260 . . #--------------------------------------- # # # AluI: # [AGCT] # 5 27 63 176 # # ApoI: # [RAATTY] # 126 145 # # AsuI*: # [GGNCC] # 112 159 # # AvaII: # [GGWCC] # 112 159 # # BmgT120I: # [GGNCC] # 113 158 # # BseSI: # [GKGCMC] # 21 250 # # BstKTI: # [GATC] # 88 183 # # BtgZI: # [GCGATG] # 83 188 # # Csp6I: # [GTAC] # 5 266 # # CviJI: # [RGCY] # 5 21 27 63 155 # # DdeI: # [CTNAG] # 28 243 # # DpnI: # [GATC] # 87 184 # # EspI*: # [GCTNAGC] # 28 243 # # FalI: # [AAGNNNNNCTT] # 0 0 16 32 223 # # HgiAI*: # [GWGCWC] # 14 257 # # HindIII: # [AAGCTT] # 30 241 # # Hpy166II: # [GTNNAC] # 5 266 # # Hpy178III*: # [TCNNGA] # 115 156 # # Hpy99I: # [CGWCG] # 86 185 # # MaeII: # [ACGT] # 103 168 # # MboI: # [GATC] # 85 186 # # MseI: # [TTAA] # 20 44 85 122 # # MwoI: # [GCNNNNNNNGC] # 38 233 # # PsiI: # [TTATAA] # 26 245 # # RsaI: # [GTAC] # 6 265 # # SduI: # [GDGCHC] # 7 14 250 # # SetI: # [ASST] # 5 29 30 33 74 100 # # TaiI: # [ACGT] # 100 171 # # TaqI: # [TCGA] # 84 187 # # TaqII: # [GACCGA] # 100 171 # # Tsp4CI*: # [ACNGT] # 16 51 204 # # TspEI: # [AATT] # 60 85 126 # # TspRI: # [CASTGNN] # 2 19 250 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 271 # Reported_sequences: 1 # Reported_hitcount: 49 #---------------------------------------