Restriction Fragments for ARS1322



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS1322 on chromosome XIII from coordinates 553422 to 554331.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.11455.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 11 + BstEII GGTNACC 5 10 . . 6 10 + Tsp45I GTSAC 5 10 . . 6 10 + MaeIII GTNAC 5 10 . . 30 33 + CviRI* TGCA 31 31 . . 48 53 + TatI WGTACW 48 52 . . 48 53 + Bsp1407I TGTACA 48 52 . . 49 52 + Csp6I GTAC 49 51 . . 49 52 + RsaI GTAC 50 50 . . 63 67 + Hpy188I TCNGA 65 64 . . 68 73 + HindIII AAGCTT 68 72 . . 69 72 + SetI ASST 72 68 . . 69 72 + CviJI RGCY 70 70 . . 69 72 + AluI AGCT 70 70 . . 76 79 + CviJI RGCY 77 77 . . 81 85 + TfiI GAWTC 81 84 . . 81 85 + HinfI GANTC 81 84 . . 84 87 + TaqI TCGA 84 86 . . 87 91 + Tsp4CI* ACNGT 89 88 . . 104 107 + CviJI RGCY 105 105 . . 111 115 + PleI GAGTC 119 120 . . 111 115 + MlyI GAGTC 120 120 . . 111 115 + HinfI GANTC 111 114 . . 114 118 + Hpy188I TCNGA 116 115 . . 121 125 + MboII GAAGA 133 132 . . 117 121 - BccI CCATC 111 112 . . 124 128 + MboII GAAGA 136 135 . . 124 129 - Ksp632I* CTCTTC 119 122 . . 124 130 - SapI GCTCTTC 119 122 . . 129 134 - BsmI GAATGC 129 127 . . 138 141 + SetI ASST 141 137 . . 139 143 + Hin4II* CCTTC 149 148 . . 141 145 - TspDTI ATGAA 131 129 . . 145 149 - MboII GAAGA 137 136 . . 152 162 + FalI AAGNNNNNCTT 143 138 175 170 154 158 - BsmAI GTCTC 148 152 . . 158 166 + DraIII CACNNNGTG 163 160 . . 160 164 + DdeI CTNAG 160 163 . . 152 162 - FalI AAGNNNNNCTT 175 170 143 138 167 170 + TaqI TCGA 167 169 . . 178 183 + VspI ATTAAT 179 181 . . 179 182 + MseI TTAA 179 181 . . 182 186 + TspDTI ATGAA 197 195 . . 189 193 + BmgT120I GGNCC 190 191 . . 189 193 + AsuI* GGNCC 189 192 . . 190 193 + CviJI RGCY 191 191 . . 190 193 + HaeIII GGCC 191 191 . . 191 195 - BceAI ACGGC 176 178 . . 198 202 - HgaI GACGC 187 192 . . 201 205 - MboII GAAGA 193 192 . . 200 205 - BbvII* GAAGAC 193 197 . . 209 212 + CviRI* TGCA 210 210 . . 210 215 + Cac8I GCNNGC 212 212 . . 230 233 + FnuDII* CGCG 231 231 . . 231 234 - AciI CCGC 231 233 . . 234 238 - Hin4II* CCTTC 228 227 . . 254 257 + MaeI CTAG 254 256 . . 258 262 + Hpy188I TCNGA 260 259 . . 265 269 + TfiI GAWTC 265 268 . . 265 269 + HinfI GANTC 265 268 . . 261 265 - BccI CCATC 255 256 . . 272 277 + SplI* CGTACG 272 276 . . 273 276 + Csp6I GTAC 273 275 . . 273 276 + RsaI GTAC 274 274 . . 278 282 + BssKI CCNGG 277 282 . . 278 282 + BseBI CCWGG 279 280 . . 278 283 + SecI* CCNNGG 278 282 . . 278 282 + ScrFI CCNGG 279 280 . . 278 282 + EcoRII CCWGG 277 282 . . 292 295 + MseI TTAA 292 294 . . 295 300 + AclI AACGTT 296 298 . . 296 299 + TaiI ACGT 299 295 . . 296 299 + SetI ASST 299 295 . . 296 299 + MaeII ACGT 296 298 . . 302 305 + CviRI* TGCA 303 303 . . 313 317 + MaeIII GTNAC 312 317 . . 318 322 + SfaNI GCATC 327 331 . . 321 325 + Hpy188I TCNGA 323 322 . . 330 333 + SetI ASST 333 329 . . 330 333 + CviJI RGCY 331 331 . . 330 333 + AluI AGCT 331 331 . . 337 340 + CviJI RGCY 338 338 . . 350 353 + TspEI AATT 349 353 . . 352 355 + MseI TTAA 352 354 . . 355 360 + ApoI RAATTY 355 359 . . 356 359 + TspEI AATT 355 359 . . 359 364 + Hpy178III* TCNNGA 360 362 . . 366 369 + FatI CATG 365 369 . . 366 369 + NlaIII CATG 369 365 . . 366 369 + CviAII CATG 366 368 . . 369 373 + TfiI GAWTC 369 372 . . 369 373 + HinfI GANTC 369 372 . . 383 387 + TspDTI ATGAA 398 396 . . 391 396 + SspI AATATT 393 393 . . 400 403 + TaqI TCGA 400 402 . . 402 405 - MnlI CCTC 395 394 . . 406 409 + FatI CATG 405 409 . . 406 409 + NlaIII CATG 409 405 . . 406 409 + CviAII CATG 406 408 . . 407 411 + TspDTI ATGAA 422 420 . . 415 419 + MboII GAAGA 427 426 . . 425 430 + Hpy178III* TCNNGA 426 428 . . 424 429 - Eco57MI CTGRAG 409 407 . . 424 429 - GsuI CTGGAG 409 407 . . 437 440 + TspEI AATT 436 440 . . 446 450 + FokI GGATG 459 463 . . 446 450 + BseGI GGATG 452 450 . . 447 457 + Hin4I GAYNNNNNVTC 438 433 470 465 448 452 + TspDTI ATGAA 463 461 . . 447 457 - Hin4I GAYNNNNNVTC 470 465 438 433 471 474 + TaiI ACGT 474 470 . . 471 474 + SetI ASST 474 470 . . 471 474 + MaeII ACGT 471 473 . . 486 490 + BinI* GGATC 494 495 . . 483 487 - Hin4II* CCTTC 477 476 . . 487 490 + DpnI GATC 488 488 . . 487 490 + BstKTI GATC 489 487 . . 487 490 + MboI GATC 486 490 . . 490 495 + MfeI CAATTG 490 494 . . 491 494 + TspEI AATT 490 494 . . 513 516 + TspEI AATT 512 516 . . 518 521 + MseI TTAA 518 520 . . 534 537 + TspEI AATT 533 537 . . 554 557 + TspEI AATT 553 557 . . 556 559 + MseI TTAA 556 558 . . 565 568 + MaeI CTAG 565 567 . . 573 578 + TatI WGTACW 573 577 . . 573 578 + ScaI AGTACT 575 575 . . 574 577 + Csp6I GTAC 574 576 . . 574 577 + RsaI GTAC 575 575 . . 578 581 + MseI TTAA 578 580 . . 585 590 + NspI RCATGY 589 585 . . 586 589 + FatI CATG 585 589 . . 586 589 + NlaIII CATG 589 585 . . 586 589 + CviAII CATG 586 588 . . 588 591 + CviRI* TGCA 589 589 . . 595 599 + Hpy188I TCNGA 597 596 . . 597 608 + BdaI TGANNNNNNTCA 586 584 620 618 601 604 + SetI ASST 604 600 . . 602 605 + MnlI CCTC 612 611 . . 597 608 - BdaI TGANNNNNNTCA 620 618 586 584 625 628 + SetI ASST 628 624 . . 625 628 + CviJI RGCY 626 626 . . 625 628 + AluI AGCT 626 626 . . 643 647 + Hin4II* CCTTC 653 652 . . 647 651 + Hpy99I CGWCG 651 646 . . 648 653 + SalI GTCGAC 648 652 . . 648 653 + Hpy166II GTNNAC 650 650 . . 648 653 + HindII GTYRAC 650 650 . . 648 653 + AccI GTMKAC 649 651 . . 649 652 + TaqI TCGA 649 651 . . 670 674 + TspDTI ATGAA 685 683 . . 676 680 + Hpy188I TCNGA 678 677 . . 680 685 + BspLU11I* ACATGT 680 684 . . 675 680 - MmeI TCCRAC 656 654 . . 680 685 + NspI RCATGY 684 680 . . 680 685 + AflIII ACRYGT 680 684 . . 681 684 + FatI CATG 680 684 . . 681 684 + NlaIII CATG 684 680 . . 681 684 + CviAII CATG 681 683 . . 691 694 + SetI ASST 694 690 . . 692 697 + StyI CCWWGG 692 696 . . 692 697 + SecI* CCNNGG 692 696 . . 699 704 + SspI AATATT 701 701 . . 704 709 + BalI TGGCCA 706 706 . . 704 709 + CfrI YGGCCR 704 708 . . 705 708 + CviJI RGCY 706 706 . . 705 708 + HaeIII GGCC 706 706 . . 711 716 + BspLU11I* ACATGT 711 715 . . 711 716 + NspI RCATGY 715 711 . . 711 716 + AflIII ACRYGT 711 715 . . 712 715 + FatI CATG 711 715 . . 712 715 + NlaIII CATG 715 711 . . 712 715 + CviAII CATG 712 714 . . 716 721 + Hpy178III* TCNNGA 717 719 . . 725 729 + TspDTI ATGAA 740 738 . . 729 732 + TspEI AATT 728 732 . . 739 743 + TfiI GAWTC 739 742 . . 739 743 + HinfI GANTC 739 742 . . 742 747 + Hpy178III* TCNNGA 743 745 . . 756 760 + TfiI GAWTC 756 759 . . 756 760 + HinfI GANTC 756 759 . . 761 764 + CviRI* TGCA 762 762 . . 762 773 + AlfI GCANNNNNNTGC 751 749 785 783 765 769 + BsrI ACTGG 770 768 . . 768 771 + CviJI RGCY 769 769 . . 769 773 + BlsI GCNGC 771 770 . . 769 773 + BisI GCNGC 770 771 . . 769 773 + TseI GCWGC 769 772 . . 770 775 + PstI CTGCAG 774 770 . . 770 775 + SfeI* CTRYAG 770 774 . . 771 774 + CviRI* TGCA 772 772 . . 769 773 - BbvI GCAGC 756 760 . . 762 773 - AlfI GCANNNNNNTGC 785 783 751 749 794 798 + MaeIII GTNAC 793 798 . . 802 806 + TspGWI ACGGA 817 815 . . 807 812 - MmeI TCCRAC 788 786 . . 813 816 + TspEI AATT 812 816 . . 816 821 + BsaAI YACGTR 818 818 . . 817 820 + TaiI ACGT 820 816 . . 817 820 + SetI ASST 820 816 . . 817 820 + MaeII ACGT 817 819 . . 819 824 + BsgI GTGCAG 840 838 . . 820 823 + CviRI* TGCA 821 821 . . 810 821 - TstI CACNNNNNNTCC 834 829 802 797 825 830 + Hpy178III* TCNNGA 826 828 . . 826 836 + BsiYI* CCNNNNNNNGG 832 829 . . 836 848 + BarI GAAGNNNNNNTAC 828 823 860 855 836 840 - Hin4II* CCTTC 830 829 . . 855 858 + TaqI TCGA 855 857 . . 873 884 + BdaI TGANNNNNNTCA 862 860 896 894 874 878 + PleI GAGTC 882 883 . . 874 878 + MlyI GAGTC 883 883 . . 874 878 + HinfI GANTC 874 877 . . 879 882 + TspEI AATT 878 882 . . 882 887 + Hpy178III* TCNNGA 883 885 . . 873 884 - BdaI TGANNNNNNTCA 896 894 862 860 886 890 + TfiI GAWTC 886 889 . . 886 890 + HinfI GANTC 886 889 . . 892 895 + FatI CATG 891 895 . . 892 895 + NlaIII CATG 895 891 . . 892 895 + CviAII CATG 892 894 . . 901 907 + PpuMI RGGWCCY 902 905 . . 901 907 + DraII RGGNCCY 902 905 . . 902 907 + NlaIV GGNNCC 904 904 . . 902 906 + BmgT120I GGNCC 903 904 . . 902 906 + AvaII GGWCC 902 905 . . 902 906 + AsuI* GGNCC 902 905 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 261 649 # # AciI: # [CCGC] # 231 679 # # AclI: # [AACGTT] # 296 614 # # AflIII: # [ACRYGT] # 31 199 680 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 125 751 # # AluI: # [AGCT] # 70 261 284 295 # # ApoI: # [RAATTY] # 355 555 # # AsuI*: # [GGNCC] # 8 189 713 # # AvaII: # [GGWCC] # 8 902 # # BalI: # [TGGCCA] # 204 706 # # BarI: # [GAAGNNNNNNTAC] # 32 50 828 # # BbvI: # [GCAGC] # 154 756 # # BbvII*: # [GAAGAC] # 193 717 # # BccI: # [CCATC] # 111 144 655 # # BceAI: # [ACGGC] # 176 734 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 14 34 # 34 242 586 # # BinI*: # [GGATC] # 416 494 # # BisI: # [GCNGC] # 140 770 # # BlsI: # [GCNGC] # 139 771 # # BmgT120I: # [GGNCC] # 7 190 713 # # BsaAI: # [YACGTR] # 92 818 # # BseBI: # [CCWGG] # 279 631 # # BseGI: # [GGATG] # 452 458 # # BsgI: # [GTGCAG] # 70 840 # # BsiYI*: # [CCNNNNNNNGG] # 78 832 # # BsmAI: # [GTCTC] # 148 762 # # BsmI: # [GAATGC] # 129 781 # # Bsp1407I: # [TGTACA] # 48 862 # # BspLU11I*: # [ACATGT] # 31 199 680 # # BsrI: # [ACTGG] # 140 770 # # BssKI: # [CCNGG] # 277 633 # # BstEII: # [GGTNACC] # 5 905 # # BstKTI: # [GATC] # 421 489 # # Cac8I: # [GCNNGC] # 212 698 # # CfrI: # [YGGCCR] # 206 704 # # Csp6I: # [GTAC] # 49 224 301 336 # # CviAII: # [CATG] # 18 31 40 95 180 180 # 366 # # CviJI: # [RGCY] # 7 7 28 63 70 80 # 86 140 141 288 # # CviRI*: # [TGCA] # 10 31 49 89 93 173 # 179 286 # # DdeI: # [CTNAG] # 160 750 # # DpnI: # [GATC] # 422 488 # # DraII: # [RGGNCCY] # 8 902 # # DraIII: # [CACNNNGTG] # 163 747 # # Eco57MI: # [CTGRAG] # 409 501 # # EcoRII: # [CCWGG] # 277 633 # # FalI: # [AAGNNNNNCTT] # 0 0 32 143 735 # # FatI: # [CATG] # 19 31 40 95 180 180 # 365 # # FnuDII*: # [CGCG] # 231 679 # # FokI: # [GGATG] # 451 459 # # GsuI: # [CTGGAG] # 409 501 # # HaeIII: # [GGCC] # 191 204 515 # # HgaI: # [GACGC] # 187 723 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 438 440 # # Hin4II*: # [CCTTC] # 79 80 149 176 177 249 # # HindII: # [GTYRAC] # 260 650 # # HindIII: # [AAGCTT] # 68 842 # # HinfI: # [GANTC] # 12 17 24 30 81 104 # 118 154 370 # # Hpy166II: # [GTNNAC] # 260 650 # # Hpy178III*: # [TCNNGA] # 26 27 57 66 83 291 # 360 # # Hpy188I: # [TCNGA] # 51 63 65 81 144 232 # 274 # # Hpy99I: # [CGWCG] # 259 651 # # Ksp632I*: # [CTCTTC] # 119 791 # # MaeI: # [CTAG] # 254 311 345 # # MaeII: # [ACGT] # 93 175 296 346 # # MaeIII: # [GTNAC] # 5 117 307 481 # # MboI: # [GATC] # 424 486 # # MboII: # [GAAGA] # 1 3 56 133 234 483 # # MfeI: # [CAATTG] # 420 490 # # MlyI: # [GAGTC] # 27 120 763 # # MmeI: # [TCCRAC] # 122 132 656 # # MnlI: # [CCTC] # 217 298 395 # # MseI: # [TTAA] # 22 38 60 113 166 179 # 332 # # NlaIII: # [CATG] # 15 31 40 95 180 180 # 369 # # NlaIV: # [GGNNCC] # 6 904 # # NspI: # [RCATGY] # 31 95 195 589 # # PleI: # [GAGTC] # 28 119 763 # # PpuMI: # [RGGWCCY] # 8 902 # # PstI: # [CTGCAG] # 136 774 # # RsaI: # [GTAC] # 50 224 301 335 # # SalI: # [GTCGAC] # 262 648 # # SapI: # [GCTCTTC] # 119 791 # # ScaI: # [AGTACT] # 335 575 # # ScrFI: # [CCNGG] # 279 631 # # SecI*: # [CCNNGG] # 218 278 414 # # SetI: # [ASST] # 24 34 66 69 72 90 # 126 130 141 158 # # SfaNI: # [GCATC] # 327 583 # # SfeI*: # [CTRYAG] # 140 770 # # SplI*: # [CGTACG] # 272 638 # # SspI: # [AATATT] # 209 308 393 # # StyI: # [CCWWGG] # 218 692 # # TaiI: # [ACGT] # 90 175 299 346 # # TaqI: # [TCGA] # 55 83 84 206 233 249 # # TatI: # [WGTACW] # 48 337 525 # # TfiI: # [GAWTC] # 17 24 81 104 130 184 # 370 # # TseI: # [GCWGC] # 141 769 # # Tsp45I: # [GTSAC] # 5 905 # # Tsp4CI*: # [ACNGT] # 89 821 # # TspDTI: # [ATGAA] # 24 41 55 66 131 170 # 201 222 # # TspEI: # [AATT] # 6 20 21 22 32 54 # 66 81 84 175 349 # # TspGWI: # [ACGGA] # 93 817 # # TstI: # [CACNNNNNNTCC] # 32 76 802 # # VspI: # [ATTAAT] # 179 731 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 910 # Reported_sequences: 1 # Reported_hitcount: 227 #---------------------------------------