Restriction Fragments for ARS1317



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS1317 on chromosome XIII from coordinates 477606 to 478301.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19314.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + Hpy166II GTNNAC 9 9 . . 7 12 + HpaI GTTAAC 9 9 . . 7 12 + HindII GTYRAC 9 9 . . 8 11 + MseI TTAA 8 10 . . 17 21 + MaeIII GTNAC 16 21 . . 30 34 + DdeI CTNAG 30 33 . . 30 34 - BspCNI CTCAG 22 20 . . 30 34 - BseMII CTCAG 21 19 . . 46 49 + DpnI GATC 47 47 . . 46 49 + BstKTI GATC 48 46 . . 46 49 + MboI GATC 45 49 . . 48 52 - MboII GAAGA 40 39 . . 51 55 - MboII GAAGA 43 42 . . 81 86 + Eco57MI CTGRAG 102 100 . . 81 86 + Eco57I CTGAAG 102 100 . . 83 87 + MboII GAAGA 95 94 . . 92 95 + FatI CATG 91 95 . . 92 95 + NlaIII CATG 95 91 . . 92 95 + CviAII CATG 92 94 . . 106 111 + AsuII TTCGAA 107 109 . . 107 110 + TaqI TCGA 107 109 . . 104 108 - MboII GAAGA 96 95 . . 113 117 + TspDTI ATGAA 128 126 . . 120 125 + SspI AATATT 122 122 . . 124 127 + MseI TTAA 124 126 . . 127 131 + Tsp4CI* ACNGT 129 128 . . 129 134 + KpnI GGTACC 133 129 . . 129 134 + NlaIV GGNNCC 131 131 . . 129 134 + HgiCI* GGYRCC 129 133 . . 129 134 + Acc65I GGTACC 129 133 . . 130 133 + Csp6I GTAC 130 132 . . 130 133 + RsaI GTAC 131 131 . . 132 135 + SetI ASST 135 131 . . 160 164 + TauI GCSGC 163 160 . . 160 164 + BlsI GCNGC 162 161 . . 160 164 + BisI GCNGC 161 162 . . 160 163 - AciI CCGC 160 162 . . 170 175 + Hpy178III* TCNNGA 171 173 . . 168 172 - FokI GGATG 154 158 . . 168 172 - BseGI GGATG 167 165 . . 172 180 + AlwNI CAGNNNCTG 177 174 . . 174 178 + TfiI GAWTC 174 177 . . 174 178 + HinfI GANTC 174 177 . . 177 181 + Hpy188I TCNGA 179 178 . . 184 188 + TfiI GAWTC 184 187 . . 184 188 + HinfI GANTC 184 187 . . 224 230 + PfoI TCCNGGA 224 229 . . 225 229 + BssKI CCNGG 224 229 . . 225 229 + BseBI CCWGG 226 227 . . 225 229 + ScrFI CCNGG 226 227 . . 225 229 + EcoRII CCWGG 224 229 . . 233 236 + CviRI* TGCA 234 234 . . 232 237 - BsgI GTGCAG 217 215 . . 252 255 + TaiI ACGT 255 251 . . 252 255 + SetI ASST 255 251 . . 252 255 + MaeII ACGT 252 254 . . 254 258 - BslFI GGGAC 239 243 . . 263 274 + TstI CACNNNNNNTCC 254 249 286 281 263 268 + BsaAI YACGTR 265 265 . . 264 267 + TaiI ACGT 267 263 . . 264 267 + SetI ASST 267 263 . . 264 267 + MaeII ACGT 264 266 . . 291 294 + Hin6I GCGC 291 293 . . 291 294 + GlaI GCGC 292 292 . . 291 294 + HhaI GCGC 293 291 . . 292 295 + FnuDII* CGCG 293 293 . . 309 314 + Hpy178III* TCNNGA 310 312 . . 313 318 + SacI GAGCTC 317 313 . . 313 318 + Ecl136II GAGCTC 315 315 . . 313 318 + SduI GDGCHC 317 313 . . 313 318 + HgiJII* GRGCYC 317 313 . . 313 318 + HgiAI* GWGCWC 317 313 . . 314 317 + SetI ASST 317 313 . . 314 317 + CviJI RGCY 315 315 . . 314 317 + AluI AGCT 315 315 . . 318 321 + AciI CCGC 318 320 . . 319 322 + FnuDII* CGCG 320 320 . . 320 323 + Hin6I GCGC 320 322 . . 320 323 + GlaI GCGC 321 321 . . 320 323 + HhaI GCGC 322 320 . . 326 331 + Ksp632I* CTCTTC 332 335 . . 327 331 - MboII GAAGA 319 318 . . 330 334 - HphI GGTGA 322 321 . . 336 339 + CviJI RGCY 337 337 . . 338 348 + BsiYI* CCNNNNNNNGG 344 341 . . 339 343 + DdeI CTNAG 339 342 . . 346 350 + BmgT120I GGNCC 347 348 . . 346 350 + AsuI* GGNCC 346 349 . . 347 350 + CviJI RGCY 348 348 . . 347 350 + HaeIII GGCC 348 348 . . 349 352 + MnlI CCTC 359 358 . . 359 362 + MaeI CTAG 359 361 . . 375 378 + CviJI RGCY 376 376 . . 375 378 + HaeIII GGCC 376 376 . . 375 379 + BmgT120I GGNCC 376 377 . . 375 379 + AsuI* GGNCC 375 378 . . 386 392 + Bpu10I CCTNAGC 387 390 . . 387 391 + DdeI CTNAG 387 390 . . 390 393 + SetI ASST 393 389 . . 390 393 + CviJI RGCY 391 391 . . 390 393 + AluI AGCT 391 391 . . 391 396 + Cac8I GCNNGC 393 393 . . 395 400 + Cac8I GCNNGC 397 397 . . 398 401 + SetI ASST 401 397 . . 398 401 + CviJI RGCY 399 399 . . 398 401 + AluI AGCT 399 399 . . 400 404 + DdeI CTNAG 400 403 . . 400 404 + BspCNI CTCAG 413 411 . . 400 404 + BseMII CTCAG 414 412 . . 399 405 - Bpu10I CCTNAGC 400 403 . . 407 411 + BlsI GCNGC 409 408 . . 407 411 + BisI GCNGC 408 409 . . 407 411 + BbvI GCAGC 419 423 . . 407 411 + TseI GCWGC 407 410 . . 408 413 + EcoP15I CAGCAG 438 440 . . 410 414 + BlsI GCNGC 412 411 . . 410 414 + BisI GCNGC 411 412 . . 410 414 + BbvI GCAGC 422 426 . . 410 414 + TseI GCWGC 410 413 . . 429 439 + BplI GAGNNNNNCTC 420 415 452 447 434 437 + AciI CCGC 434 436 . . 434 439 + BsrBI CCGCTC 436 436 . . 438 441 + TaqI TCGA 438 440 . . 429 439 - BplI GAGNNNNNCTC 452 447 420 415 441 444 + SetI ASST 444 440 . . 442 445 + MnlI CCTC 452 451 . . 447 451 + TspGWI ACGGA 462 460 . . 449 453 + FokI GGATG 462 466 . . 449 453 + BseGI GGATG 455 453 . . 467 472 + TatI WGTACW 467 471 . . 468 471 + Csp6I GTAC 468 470 . . 468 471 + RsaI GTAC 469 469 . . 479 491 + PsrI GAACNNNNNNTAC 471 466 503 498 481 484 + TaiI ACGT 484 480 . . 481 484 + SetI ASST 484 480 . . 481 484 + MaeII ACGT 481 483 . . 485 488 + MnlI CCTC 495 494 . . 494 497 + TaiI ACGT 497 493 . . 494 497 + SetI ASST 497 493 . . 494 497 + MaeII ACGT 494 496 . . 509 512 + CviRI* TGCA 510 510 . . 540 543 + SetI ASST 543 539 . . 538 542 - Hin4II* CCTTC 532 531 . . 550 553 - MnlI CCTC 543 542 . . 561 564 + SetI ASST 564 560 . . 561 566 + BspMI ACCTGC 570 574 . . 564 567 + CviRI* TGCA 565 565 . . 566 569 + FatI CATG 565 569 . . 566 569 + NlaIII CATG 569 565 . . 566 569 + CviAII CATG 566 568 . . 569 572 + CviJI RGCY 570 570 . . 571 574 + MaeI CTAG 571 573 . . 568 573 - TsoI TARCCA 558 556 . . 583 587 + MaeIII GTNAC 582 587 . . 589 592 + SetI ASST 592 588 . . 591 595 + MaeIII GTNAC 590 595 . . 598 601 + CviRI* TGCA 599 599 . . 617 620 + TspEI AATT 616 620 . . 626 630 + FauI CCCGC 634 636 . . 627 630 + AciI CCGC 627 629 . . 650 653 + MseI TTAA 650 652 . . 653 657 + Tsp4CI* ACNGT 655 654 . . 658 662 + HphI GGTGA 670 669 . . 659 663 + Tsp45I GTSAC 658 663 . . 659 663 + MaeIII GTNAC 658 663 . . 680 684 + BseGI GGATG 686 684 . . 689 692 + SetI ASST 692 688 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 129 567 # # AciI: # [CCGC] # 69 116 158 160 193 # # AluI: # [AGCT] # 8 76 297 315 # # AlwNI: # [CAGNNNCTG] # 177 519 # # AsuI*: # [GGNCC] # 29 321 346 # # AsuII: # [TTCGAA] # 107 589 # # BbvI: # [GCAGC] # 3 274 419 # # BisI: # [GCNGC] # 3 161 247 285 # # BlsI: # [GCNGC] # 3 162 247 284 # # BmgT120I: # [GGNCC] # 29 320 347 # # BplI: # [GAGNNNNNCTC] # 0 0 32 244 420 # # Bpu10I: # [CCTNAGC] # 13 296 387 # # BsaAI: # [YACGTR] # 265 431 # # BseBI: # [CCWGG] # 226 470 # # BseGI: # [GGATG] # 10 167 231 288 # # BseMII: # [CTCAG] # 21 282 393 # # BsgI: # [GTGCAG] # 217 479 # # BsiYI*: # [CCNNNNNNNGG] # 344 352 # # BslFI: # [GGGAC] # 239 457 # # BspCNI: # [CTCAG] # 22 283 391 # # BspMI: # [ACCTGC] # 126 570 # # BsrBI: # [CCGCTC] # 260 436 # # BssKI: # [CCNGG] # 224 472 # # BstKTI: # [GATC] # 48 648 # # Cac8I: # [GCNNGC] # 4 299 393 # # Csp6I: # [GTAC] # 130 228 338 # # CviAII: # [CATG] # 92 130 474 # # CviJI: # [RGCY] # 8 11 15 22 28 126 # 171 315 # # CviRI*: # [TGCA] # 34 55 97 234 276 # # DdeI: # [CTNAG] # 13 30 48 296 309 # # DpnI: # [GATC] # 47 649 # # Ecl136II: # [GAGCTC] # 315 381 # # Eco57I: # [CTGAAG] # 102 594 # # Eco57MI: # [CTGRAG] # 102 594 # # EcoP15I: # [CAGCAG] # 258 438 # # EcoRII: # [CCWGG] # 224 472 # # FatI: # [CATG] # 91 131 474 # # FauI: # [CCCGC] # 62 634 # # FnuDII*: # [CGCG] # 27 293 376 # # FokI: # [GGATG] # 154 234 308 # # GlaI: # [GCGC] # 29 292 375 # # HaeIII: # [GGCC] # 28 320 348 # # HgiAI*: # [GWGCWC] # 317 379 # # HgiCI*: # [GGYRCC] # 129 567 # # HgiJII*: # [GRGCYC] # 317 379 # # HhaI: # [GCGC] # 29 293 374 # # Hin4II*: # [CCTTC] # 164 532 # # Hin6I: # [GCGC] # 29 291 376 # # HindII: # [GTYRAC] # 9 687 # # HinfI: # [GANTC] # 10 174 512 # # HpaI: # [GTTAAC] # 9 687 # # HphI: # [GGTGA] # 26 322 348 # # Hpy166II: # [GTNNAC] # 9 687 # # Hpy178III*: # [TCNNGA] # 139 171 386 # # Hpy188I: # [TCNGA] # 179 517 # # KpnI: # [GGTACC] # 133 563 # # Ksp632I*: # [CTCTTC] # 332 364 # # MaeI: # [CTAG] # 125 212 359 # # MaeII: # [ACGT] # 12 13 202 217 252 # # MaeIII: # [GTNAC] # 8 16 38 68 566 # # MboI: # [GATC] # 45 651 # # MboII: # [GAAGA] # 1 3 40 52 223 377 # # MnlI: # [CCTC] # 43 48 93 153 359 # # MseI: # [TTAA] # 8 46 116 526 # # NlaIII: # [CATG] # 95 127 474 # # NlaIV: # [GGNNCC] # 131 565 # # PfoI: # [TCCNGGA] # 224 472 # # PsrI: # [GAACNNNNNNTAC] # 32 193 471 # # RsaI: # [GTAC] # 131 227 338 # # SacI: # [GAGCTC] # 317 379 # # ScrFI: # [CCNGG] # 226 470 # # SduI: # [GDGCHC] # 317 379 # # SetI: # [ASST] # 4 8 12 13 21 28 # 40 43 46 50 76 100 # 120 135 # # SspI: # [AATATT] # 122 574 # # TaiI: # [ACGT] # 12 13 199 217 255 # # TaqI: # [TCGA] # 107 258 331 # # TatI: # [WGTACW] # 229 467 # # TauI: # [GCSGC] # 163 533 # # TfiI: # [GAWTC] # 10 174 512 # # TseI: # [GCWGC] # 3 286 407 # # TsoI: # [TARCCA] # 138 558 # # Tsp45I: # [GTSAC] # 38 658 # # Tsp4CI*: # [ACNGT] # 41 129 526 # # TspDTI: # [ATGAA] # 128 568 # # TspEI: # [AATT] # 80 616 # # TspGWI: # [ACGGA] # 234 462 # # TstI: # [CACNNNNNNTCC] # 32 254 410 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 696 # Reported_sequences: 1 # Reported_hitcount: 167 #---------------------------------------