Restriction Fragments for ARS1232



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS1232 on chromosome XII from coordinates 1007186 to 1007475.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.20954.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + BsaAI YACGTR 8 8 . . 7 10 + TaiI ACGT 10 6 . . 7 10 + SetI ASST 10 6 . . 7 10 + MaeII ACGT 7 9 . . 11 15 + MboII GAAGA 23 22 . . 26 30 - MboII GAAGA 18 17 . . 32 37 + BspLU11I* ACATGT 32 36 . . 32 37 + NspI RCATGY 36 32 . . 32 37 + AflIII ACRYGT 32 36 . . 33 36 + FatI CATG 32 36 . . 33 36 + NlaIII CATG 36 32 . . 33 36 + CviAII CATG 33 35 . . 51 55 - TspDTI ATGAA 41 39 . . 76 80 + DdeI CTNAG 76 79 . . 79 82 + SetI ASST 82 78 . . 75 81 - Bpu10I CCTNAGC 76 79 . . 93 96 + SetI ASST 96 92 . . 93 96 + CviJI RGCY 94 94 . . 93 96 + AluI AGCT 94 94 . . 95 99 + DdeI CTNAG 95 98 . . 100 103 + MseI TTAA 100 102 . . 104 107 + SetI ASST 107 103 . . 104 107 + CviJI RGCY 105 105 . . 104 107 + AluI AGCT 105 105 . . 106 110 + DdeI CTNAG 106 109 . . 132 135 + TspEI AATT 131 135 . . 142 145 + TspEI AATT 141 145 . . 144 149 + PsiI TTATAA 146 146 . . 148 153 + ApoI RAATTY 148 152 . . 149 152 + TspEI AATT 148 152 . . 156 159 + Csp6I GTAC 156 158 . . 156 159 + RsaI GTAC 157 157 . . 168 173 + Hpy178III* TCNNGA 169 171 . . 168 173 + BspHI TCATGA 168 172 . . 169 172 + FatI CATG 168 172 . . 169 172 + NlaIII CATG 172 168 . . 169 172 + CviAII CATG 169 171 . . 173 176 + SetI ASST 176 172 . . 172 175 - MnlI CCTC 165 164 . . 182 187 + AhaIII* TTTAAA 184 184 . . 183 186 + MseI TTAA 183 185 . . 208 212 - MboII GAAGA 200 199 . . 226 231 + ApoI RAATTY 226 230 . . 227 230 + TspEI AATT 226 230 . . 248 258 + BsiYI* CCNNNNNNNGG 254 251 . . 249 252 + MnlI CCTC 259 258 . . 251 256 + XbaI TCTAGA 251 255 . . 251 256 + Hpy178III* TCNNGA 252 254 . . 252 255 + MaeI CTAG 252 254 . . 256 259 + SetI ASST 259 255 . . 255 258 - MnlI CCTC 248 247 . . 265 268 + MseI TTAA 265 267 . . 268 272 + Tsp4CI* ACNGT 270 269 . . 284 289 + ApoI RAATTY 284 288 . . 285 288 + TspEI AATT 284 288 . . #--------------------------------------- # # # AflIII: # [ACRYGT] # 32 258 # # AhaIII*: # [TTTAAA] # 106 184 # # AluI: # [AGCT] # 11 94 185 # # ApoI: # [RAATTY] # 6 58 78 148 # # Bpu10I: # [CCTNAGC] # 76 214 # # BsaAI: # [YACGTR] # 8 282 # # BsiYI*: # [CCNNNNNNNGG] # 36 254 # # BspHI: # [TCATGA] # 122 168 # # BspLU11I*: # [ACATGT] # 32 258 # # Csp6I: # [GTAC] # 134 156 # # CviAII: # [CATG] # 33 121 136 # # CviJI: # [RGCY] # 11 94 185 # # DdeI: # [CTNAG] # 11 19 76 184 # # FatI: # [CATG] # 32 122 136 # # Hpy178III*: # [TCNNGA] # 38 83 169 # # MaeI: # [CTAG] # 38 252 # # MaeII: # [ACGT] # 7 283 # # MboII: # [GAAGA] # 5 18 90 177 # # MnlI: # [CCTC] # 11 31 83 165 # # MseI: # [TTAA] # 25 82 83 100 # # NlaIII: # [CATG] # 36 118 136 # # NspI: # [RCATGY] # 36 254 # # PsiI: # [TTATAA] # 144 146 # # RsaI: # [GTAC] # 133 157 # # SetI: # [ASST] # 10 11 14 31 69 72 # 83 # # TaiI: # [ACGT] # 10 280 # # Tsp4CI*: # [ACNGT] # 20 270 # # TspDTI: # [ATGAA] # 41 249 # # TspEI: # [AATT] # 6 7 10 58 78 131 # # XbaI: # [TCTAGA] # 39 251 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 290 # Reported_sequences: 1 # Reported_hitcount: 55 #---------------------------------------