Restriction Fragments for ARS1112



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS1112 on chromosome XI from coordinates 388970 to 389264.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30128.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 7 + Hpy188I TCNGA 5 4 . . 6 10 + HinfI GANTC 6 9 . . 39 43 - MboII GAAGA 31 30 . . 41 45 - TspDTI ATGAA 31 29 . . 76 79 + TspEI AATT 75 79 . . 87 90 + MseI TTAA 87 89 . . 89 92 + TspEI AATT 88 92 . . 93 98 + Hpy178III* TCNNGA 94 96 . . 112 117 + ApoI RAATTY 112 116 . . 113 116 + TspEI AATT 112 116 . . 117 121 + Hpy188I TCNGA 119 118 . . 134 137 + MaeI CTAG 134 136 . . 143 146 + TspEI AATT 142 146 . . 145 148 + MseI TTAA 145 147 . . 153 156 - MnlI CCTC 146 145 . . 156 161 + NspI RCATGY 160 156 . . 156 161 + Cac8I GCNNGC 158 158 . . 156 161 + SphI GCATGC 160 156 . . 157 160 + FatI CATG 156 160 . . 157 160 + NlaIII CATG 160 156 . . 157 160 + CviAII CATG 157 159 . . 161 165 + Hpy99I CGWCG 165 160 . . 163 167 + TspGWI ACGGA 178 176 . . 172 177 + SspI AATATT 174 174 . . 177 188 + BdaI TGANNNNNNTCA 166 164 200 198 177 188 - BdaI TGANNNNNNTCA 200 198 166 164 190 193 + SetI ASST 193 189 . . 190 193 + CviJI RGCY 191 191 . . 190 193 + AluI AGCT 191 191 . . 197 207 + BsiYI* CCNNNNNNNGG 203 200 . . 198 204 + TspRI CASTGNN 204 195 . . 202 211 + XmnI GAANNNNTTC 206 206 . . 220 223 + TspEI AATT 219 223 . . 224 228 - TspDTI ATGAA 214 212 . . 233 236 + MseI TTAA 233 235 . . 256 259 + SetI ASST 259 255 . . 266 270 - MboII GAAGA 258 257 . . 270 274 + DdeI CTNAG 270 273 . . #--------------------------------------- # # # AluI: # [AGCT] # 104 191 # # ApoI: # [RAATTY] # 112 183 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 95 166 # # BsiYI*: # [CCNNNNNNNGG] # 92 203 # # Cac8I: # [GCNNGC] # 137 158 # # CviAII: # [CATG] # 138 157 # # CviJI: # [RGCY] # 104 191 # # DdeI: # [CTNAG] # 25 270 # # FatI: # [CATG] # 139 156 # # HinfI: # [GANTC] # 6 289 # # Hpy178III*: # [TCNNGA] # 94 201 # # Hpy188I: # [TCNGA] # 5 114 176 # # Hpy99I: # [CGWCG] # 130 165 # # MaeI: # [CTAG] # 134 161 # # MboII: # [GAAGA] # 31 37 227 # # MnlI: # [CCTC] # 146 149 # # MseI: # [TTAA] # 58 62 87 88 # # NlaIII: # [CATG] # 135 160 # # NspI: # [RCATGY] # 135 160 # # SetI: # [ASST] # 36 66 193 # # SphI: # [GCATGC] # 135 160 # # SspI: # [AATATT] # 121 174 # # TspDTI: # [ATGAA] # 31 81 183 # # TspEI: # [AATT] # 13 24 30 75 76 77 # # TspGWI: # [ACGGA] # 117 178 # # TspRI: # [CASTGNN] # 91 204 # # XmnI: # [GAANNNNTTC] # 89 206 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 295 # Reported_sequences: 1 # Reported_hitcount: 38 #---------------------------------------