Restriction Fragments for ARS101/ARS109



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS101/ARS109 on chromosome I from coordinates 159907 to 160127.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22989.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + NspBII* CMGCKG 6 6 . . 6 9 - AciI CCGC 6 8 . . 28 31 + MaeI CTAG 28 30 . . 38 41 + TspEI AATT 37 41 . . 70 74 + Tsp4CI* ACNGT 72 71 . . 84 89 + Hpy166II GTNNAC 86 86 . . 105 108 + SetI ASST 108 104 . . 105 108 + CviJI RGCY 106 106 . . 105 108 + AluI AGCT 106 106 . . 106 112 + SapI GCTCTTC 113 116 . . 107 112 + Ksp632I* CTCTTC 113 116 . . 108 112 - MboII GAAGA 100 99 . . 116 120 + Tsp4CI* ACNGT 118 117 . . 119 124 + Hpy166II GTNNAC 121 121 . . 119 124 + HindII GTYRAC 121 121 . . 124 128 - FokI GGATG 110 114 . . 124 128 - BseGI GGATG 123 121 . . 127 131 - BsrI ACTGG 127 125 . . 131 135 - TspDTI ATGAA 121 119 . . 143 148 + BsaAI YACGTR 145 145 . . 143 148 + PmaCI CACGTG 145 145 . . 144 147 + TaiI ACGT 147 143 . . 144 147 + SetI ASST 147 143 . . 144 147 + MaeII ACGT 144 146 . . 144 149 + AflIII ACRYGT 144 148 . . 156 160 - Hin4II* CCTTC 150 149 . . 165 170 + SspI AATATT 167 167 . . 174 178 + SfaNI GCATC 183 187 . . 180 184 - TspDTI ATGAA 170 168 . . 191 194 + TspEI AATT 190 194 . . 205 210 + ClaI ATCGAT 206 208 . . 206 209 + TaqI TCGA 206 208 . . 208 211 + DpnI GATC 209 209 . . 208 211 + BstKTI GATC 210 208 . . 208 211 + MboI GATC 207 211 . . 211 216 + SecI* CCNNGG 211 215 . . 208 212 - BinI* GGATC 202 203 . . 213 217 + Hpy188I TCNGA 215 214 . . #--------------------------------------- # # # AciI: # [CCGC] # 6 215 # # AflIII: # [ACRYGT] # 77 144 # # AluI: # [AGCT] # 106 115 # # BinI*: # [GGATC] # 19 202 # # BsaAI: # [YACGTR] # 76 145 # # BseGI: # [GGATG] # 98 123 # # BsrI: # [ACTGG] # 94 127 # # BstKTI: # [GATC] # 11 210 # # ClaI: # [ATCGAT] # 15 206 # # CviJI: # [RGCY] # 106 115 # # DpnI: # [GATC] # 12 209 # # FokI: # [GGATG] # 110 111 # # Hin4II*: # [CCTTC] # 71 150 # # HindII: # [GTYRAC] # 100 121 # # Hpy166II: # [GTNNAC] # 35 86 100 # # Hpy188I: # [TCNGA] # 6 215 # # Ksp632I*: # [CTCTTC] # 108 113 # # MaeI: # [CTAG] # 28 193 # # MaeII: # [ACGT] # 77 144 # # MboI: # [GATC] # 14 207 # # MboII: # [GAAGA] # 100 121 # # NspBII*: # [CMGCKG] # 6 215 # # PmaCI: # [CACGTG] # 76 145 # # SapI: # [GCTCTTC] # 108 113 # # SecI*: # [CCNNGG] # 10 211 # # SetI: # [ASST] # 39 74 108 # # SfaNI: # [GCATC] # 38 183 # # SspI: # [AATATT] # 54 167 # # TaiI: # [ACGT] # 74 147 # # TaqI: # [TCGA] # 15 206 # # Tsp4CI*: # [ACNGT] # 46 72 103 # # TspDTI: # [ATGAA] # 49 51 121 # # TspEI: # [AATT] # 31 37 153 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 221 # Reported_sequences: 1 # Reported_hitcount: 38 #---------------------------------------