Restriction Fragments for ARS104



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ARS104 on chromosome I from coordinates 30946 to 31183.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.12081.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 15 19 - MboII GAAGA 7 6 . . 26 35 + MslI CAYNNNNRTG 30 30 . . 36 40 + BlsI GCNGC 38 37 . . 36 40 + BisI GCNGC 37 38 . . 36 40 + BbvI GCAGC 48 52 . . 36 40 + TseI GCWGC 36 39 . . 38 41 + SetI ASST 41 37 . . 38 41 + CviJI RGCY 39 39 . . 38 41 + AluI AGCT 39 39 . . 61 64 + MseI TTAA 61 63 . . 74 79 + Eco31I GGTCTC 80 84 . . 75 79 + BsmAI GTCTC 80 84 . . 78 82 + Hpy188I TCNGA 80 79 . . 79 83 + DdeI CTNAG 79 82 . . 80 91 + BdaI TGANNNNNNTCA 69 67 103 101 79 83 - BspCNI CTCAG 71 69 . . 79 83 - BseMII CTCAG 70 68 . . 80 91 - BdaI TGANNNNNNTCA 103 101 69 67 119 123 + TspGWI ACGGA 134 132 . . 122 127 + SduI GDGCHC 126 122 . . 122 127 + HgiAI* GWGCWC 126 122 . . 138 141 + TspEI AATT 137 141 . . 140 143 + MseI TTAA 140 142 . . 150 153 + MseI TTAA 150 152 . . 157 161 - Hin4II* CCTTC 151 150 . . 161 171 + MwoI GCNNNNNNNGC 167 164 . . 167 170 - MnlI CCTC 160 159 . . 202 205 + CviJI RGCY 203 203 . . 203 207 + BlsI GCNGC 205 204 . . 203 207 + BisI GCNGC 204 205 . . 203 207 + TseI GCWGC 203 206 . . 203 207 - BbvI GCAGC 190 194 . . 204 209 - EcoP15I CAGCAG 176 178 . . 220 223 + CviRI* TGCA 221 221 . . 219 224 - BsgI GTGCAG 204 202 . . 225 228 + MseI TTAA 225 227 . . #--------------------------------------- # # # AluI: # [AGCT] # 39 199 # # BbvI: # [GCAGC] # 48 48 142 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 69 135 # # BisI: # [GCNGC] # 34 37 167 # # BlsI: # [GCNGC] # 33 38 167 # # BseMII: # [CTCAG] # 70 168 # # BsgI: # [GTGCAG] # 34 204 # # BsmAI: # [GTCTC] # 80 158 # # BspCNI: # [CTCAG] # 71 167 # # CviJI: # [RGCY] # 35 39 164 # # CviRI*: # [TGCA] # 17 221 # # DdeI: # [CTNAG] # 79 159 # # Eco31I: # [GGTCTC] # 80 158 # # EcoP15I: # [CAGCAG] # 62 176 # # HgiAI*: # [GWGCWC] # 112 126 # # Hin4II*: # [CCTTC] # 87 151 # # Hpy188I: # [TCNGA] # 80 158 # # MboII: # [GAAGA] # 7 231 # # MnlI: # [CCTC] # 78 160 # # MseI: # [TTAA] # 10 13 61 75 79 # # MslI: # [CAYNNNNRTG] # 30 208 # # MwoI: # [GCNNNNNNNGC] # 71 167 # # SduI: # [GDGCHC] # 112 126 # # SetI: # [ASST] # 41 197 # # TseI: # [GCWGC] # 35 36 167 # # TspEI: # [AATT] # 101 137 # # TspGWI: # [ACGGA] # 104 134 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 238 # Reported_sequences: 1 # Reported_hitcount: 36 #---------------------------------------