Lecinski S, et al. (2022) Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes: in vitro and in vivo. Interface Focus 12(6):20220042 PMID:36330320
Payne-Dwyer AL and Leake MC (2022) Single-Molecular Quantification of Flowering Control Proteins Within Nuclear Condensates in Live Whole Arabidopsis Root. Methods Mol Biol 2476:311-328 PMID:35635712
Schnitzer B, et al. (2022) The effect of stress on biophysical characteristics of misfolded protein aggregates in living Saccharomyces cerevisiae cells. Exp Gerontol 162:111755 PMID:35240259
Shashkova S, et al. (2022) Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells. Methods Mol Biol 2476:183-190 PMID:35635705
Wollman AJM and Leake MC (2022) Single-Molecule Narrow-Field Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells. Methods Mol Biol 2476:5-16 PMID:35635693
Laidlaw KME, et al. (2021) A glucose-starvation response governs endocytic trafficking and eisosomal retention of surface cargoes in budding yeast. J Cell Sci 134(2) PMID:33443082
Lecinski S, et al. (2021) Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET. Curr Top Membr 88:75-118 PMID:34862033
Shashkova S, et al. (2021) Correlating single-molecule characteristics of the yeast aquaglyceroporin Fps1 with environmental perturbations directly in living cells. Methods 193:46-53 PMID:32387484
Shepherd JW, et al. (2021) Molecular crowding in single eukaryotic cells: Using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number. Methods 193:54-61 PMID:33157192
Wollman AJM, et al. (2020) Towards mapping the 3D genome through high speed single-molecule tracking of functional transcription factors in single living cells. Methods 170:82-89 PMID:31252059
Leake MC (2018) Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding. Transcription 9(5):298-306 PMID:29895219
Shashkova S, et al. (2018) Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae. Bio Protoc 8(2):e2710 PMID:29430484
Shashkova S, et al. (2017) The yeast Mig1 transcriptional repressor is dephosphorylated by glucose-dependent and -independent mechanisms. FEMS Microbiol Lett 364(14) PMID:28854669
Wollman AJ and Leake MC (2016) Single-Molecule Narrow-Field Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells. Methods Mol Biol 1431:5-15 PMID:27283298
Wollman AJ and Leake MC (2015) Millisecond single-molecule localization microscopy combined with convolution analysis and automated image segmentation to determine protein concentrations in complexly structured, functional cells, one cell at a time. Faraday Discuss 184:401-24 PMID:26419209