Recombinant overexpression of a protein or a protein complex using any specific heterologous host can be an overwhelming challenge. The reasons may range from low yield and poor solubility of a single-subunit enzyme to the wrong stoichiometry or the incomplete assembly of a multiprotein complex. Whatever the reason, overcoming the difficulties will take the researcher into a journey through the seemingly countless options that exist for protein expression. While some choices stand to reason fairly straightforwardly (e.g., using Escherichia coli for the production of bacterial enzymes), most other choices do not need to be so self-revealing. Here, we attempt to portrait the canvas of available hosts for heterologous expression of many different protein classes and complexes and offer guidance as to which expression host may be more suitable to the problem at hand. The guidance in this chapter must be taken only as a rough indication which will have to be checked against the available literature and corroborated by experiment. It is not only expected but also welcome that, as more knowledge is gathered about the performance of hosts and protein types and new expression systems develop, the information in this chapter will have to be updated and refined.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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