Site-specific recombinases are widely used for selectable marker recycling in molecular-genetic manipulations with eukaryotic cells. This usually involves the use of two genetic constructs, one of which possesses a selectable marker flanked by the recombinase recognition sequences, while the other one bears the recombinase gene. Combining the recombinase gene with its recognition sequences in one plasmid is usually avoided, as it may lead to undesirable recombination due to promoter leakage, while the plasmid is maintained in Escherichia coli cells. Here, we describe yeast vectors possessing Cre recombinase genes under control of regulatable yeast promoters and loxP sequences for the in vivo vector backbone excision. The plasmid stability in E. coli is ensured by the presence of an intron in the recombinase gene. Applicability of these vectors was validated by disruptions of the Hansenula polymorpha PMC1 and Saccharomyces cerevisiae HSP104 and PRB1 genes.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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