Reference: Galván Márquez I, et al. (2013) Disruption of protein synthesis as antifungal mode of action by chitosan. Int J Food Microbiol 164(1):108-12

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Abstract


The antimicrobial activity of chitosan has been acknowledged for more than 30 years and yet its mode-of-action remains ambiguous. We analyzed chemical-genetic interactions of low-molecular weight chitosan using a collection of ≈ 4600 S. cerevisiae deletion mutants and found that 31% of the 107 mutants most sensitive to chitosan had deletions of genes related primarily to functions involving protein synthesis. Disruption of protein synthesis by chitosan was substantiated by an in vivo β-galactosidase expression assay suggesting that this is a primary mode of antifungal action. Analysis of the yeast gene deletion array and secondary assays also indicate that chitosan has a minor membrane disruption effect - a leading model of chitosan antimicrobial activity.

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Journal Article | Research Support, Non-U.S. Gov't
Authors
Galván Márquez I, Akuaku J, Cruz I, Cheetham J, Golshani A, Smith ML
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