Reference: Reitman ZJ, et al. (2012) Enzyme redesign guided by cancer-derived IDH1 mutations. Nat Chem Biol 8(11):887-9

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Abstract


Mutations in an enzyme can result in a neomorphic catalytic activity in cancers. We applied cancer-associated mutations from isocitrate dehydrogenases to homologous residues in the active sites of homoisocitrate dehydrogenases to derive enzymes that catalyze the conversion of 2-oxoadipate to (R)-2-hydroxyadipate, a critical step for adipic acid production. Thus, we provide a prototypic example of how insights from cancer genome sequencing and functional studies can aid in enzyme redesign.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Reitman ZJ, Choi BD, Spasojevic I, Bigner DD, Sampson JH, Yan H
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