Ethanol was produced at good rates by direct saccharification and fermentation of cello-oligosaccharides with pYBGA1 yeast, a recombinant laboratory yeast expressing β-glucosidase. Cellobiose in the concentration of 50 g/L was directly fermented for 60 h with 1×10(8) cells/mL of pYBGA1 yeast at 30 °C to give ethanol at an 80% theoretical conversion rate and a concentration of more than 20 g/L of concentration. Conversion to ethanol increased with increasing cellobiose concentration in the feed. When cellobiose was used at the concentration of 100g/L, ethanol conversion and concentration increased to 85% and 45 g/L, respectively, in 96 h incubation. Other cello-oligosaccharides, cellotriose, cellotetraose, and cellopentaose at the concentration of 50 g/L, respectively, were also fermented directly for 72 h with 1×10(8) cells/mL of pYBGA1 yeast to produce ethanol in the conversion rates and concentrations of 71-73% and 18.0-18.5 g/L, respectively. The direct saccharification and fermentation mechanism of cello-oligosaccharides with pYBGA1 yeast, as revealed by HPLC measurements, suggesting that cellotetraose, for example, was saccharificated to cellotriose, cellobiose, and glucose and then fermented to give ethanol. These results suggest that the direct saccharification and fermentation of cello-oligosaccharides with pYBGA1 has several advantages as a simple procedure and for time, cost, and energy consumptions.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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