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Reference: Ezeokonkwo C, et al. (2012) Novel interactions at the essential N-terminus of poly(A) polymerase that could regulate poly(A) addition in Saccharomyces cerevisiae. FEBS Lett 586(8):1173-8

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Abstract

Addition of poly(A) to the 3' ends of cleaved pre-mRNA is essential for mRNA maturation and is catalyzed by Pap1 in yeast. We have previously shown that a non-viable Pap1 mutant lacking the first 18 amino acids is fully active for polyadenylation of oligoA, but defective for pre-mRNA polyadenylation, suggesting that interactions at the N-terminus are important for enzyme function in the processing complex. We have now identified proteins that interact specifically with this region. Cft1 and Pta1 are subunits of the cleavage/polyadenylation factor, in which Pap1 resides, and Nab6 and Sub1 are nucleic-acid binding proteins with known links to 3' end processing. Our results suggest a novel mechanism for controlling Pap1 activity, and possible models invoking these newly-discovered interactions are discussed.CI - Copyright (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Reference Type
Journal Article | Research Support, N.I.H., Extramural
Authors
Ezeokonkwo C, Ghazy MA, Zhelkovsky A, Yeh PC, Moore C
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