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Reference: Nyathi Y, et al. (2012) Pseudo half-molecules of the ABC transporter, COMATOSE, bind Pex19 and target to peroxisomes independently but are both required for activity. FEBS Lett 586(16):2280-6

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Abstract

Peroxisomal ABC transporters of animals and fungi are "half-size" proteins which dimerise to form a functional transporter. However, peroxisomal ABC transporters of land plants are synthesised as a single polypeptide which represents a fused heterodimer. The N- and C-terminal pseudo-halves of COMATOSE (CTS; AtABCD1) were expressed as separate polypeptides which bound Pex19 in vitro and targeted independently to the peroxisome membrane in yeast, where they were stable but not functional. When co-expressed, the pseudo-halves were fully functional as indicated by ATPase activity and rescue of the pxa1pxa2? mutant for growth on oleate. The functional significance of heterodimer asymmetry is discussed.

Reference Type
Journal Article
Authors
Nyathi Y, Zhang X, Baldwin JM, Bernhardt K, Johnson B, Baldwin SA, Theodoulou FL, Baker A
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